Review
Chemistry, Multidisciplinary
Guocheng Fang, Yu-Cheng Chen, Hongxu Lu, Dayong Jin
Summary: Multicellular spheroids and organoids are promising models in personalized medicine and drug screening, replicating the structural and functional characteristics of human organs. Microfluidic technology and micro-nano fabrication meet the high requirements of engineering approaches in spheroids and organoids research. This review discusses how spheroids- and organoids-on-a-chip technology facilitates their establishment, expansion, and application through spatial-temporal control, mechanical cues modeling, high-throughput analysis, co-culture, multi-tissue interactions, biosensing, and bioimaging integration. The potential opportunities and challenges in developing spheroids- and organoids-on-a-chip technology are also highlighted.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Health Care Sciences & Services
Masahiro Shiihara, Toru Furukawa
Summary: Cell models play a crucial role in the research and development of cancer therapies. Recent advances in patient-derived cancer organoids have provided a valuable platform for studying cancer biology and personalized medicine, particularly in the field of pancreatobiliary cancer.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Oncology
Yuenan Wang, Ye Li, Zonghai Sheng, Weiwei Deng, Hongyan Yuan, Shubin Wang, Yajie Liu
Summary: Patient-derived organoids (PDO) technology provides more relevant cancer models for developing and optimizing therapeutic strategies, especially in combining radiotherapy with other treatments and studying radiation dose escalation. This review introduces the recent advances in PDO technology and personalized radiotherapy, and examines its applications in radiation therapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Materials Science, Biomaterials
Shaohua Ma, Haoran Zhao, Edgar A. Galan
Summary: Organoid technology has advanced rapidly, but its practical applications are still limited. Integrating engineering and artificial intelligence into research practices may accelerate the translation of organoids, with organoids-on-a-chip proposed as an ideal platform for achieving high-throughput, automated manipulation, and intelligent monitoring and control. Once established and adopted by the scientific community, rapid translation of organoids from laboratories to clinics and pharmaceutical industry may be realized.
Article
Engineering, Environmental
Yujuan Zhu, Lingyu Sun, Xiao Fu, Junying Liu, Zhenjiang Liang, Hui Tan, Weiping Li, Yuanjin Zhao
Summary: Our multidisciplinary strategy involves engineering vascularized human induced pluripotent stem cells (hiPSCs)-derived brain organoids system with biomimetic features using microfluidic hydrogel microcapsules. The precise fluids control of microfluidic electrospray technology enables the efficient encapsulation of hiPSCs-derived neural cells and formation of uniform human brain organoids. The resulting vascularized brain organoids exhibit structured organization, reasonable tissue size, in vivo-like neural identity, brain region, layered cortex, and complex vascular networks.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Biomedical
Elisabeth Prince, Sina Kheiri, Yihe Wang, Fei Xu, Jennifer Cruickshank, Valentina Topolskaia, Huachen Tao, Edmond W. K. Young, Alison P. McGuigan, David W. Cescon, Eugenia Kumacheva
Summary: The study introduces a novel microfluidic platform for efficient studies on the effect of drug dose and supply rate on chemosensitivity of breast tumor spheroids, and demonstrates the capability to grow large arrays of tumor spheroids from patient-derived cells of different breast cancer subtypes.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Oncology
Zhen Fang, Peijuan Li, Fengying Du, Liang Shang, Leping Li
Summary: Organoids are established through in vitro 3D culture and have the ability to mimic the structure and physiological functions of organs or tissues in vivo. They have gained significant attention and are considered reliable platforms for cancer research and treatment. Organoids have great potential in areas such as new drug development, drug screening, tumor mechanism research, and precision medicine. This review primarily focuses on recent advances in the application of organoids in cancer research and discusses the opportunities and challenges they face, aiming to provide insights for their future development.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Review
Cell Biology
Junzhe Zhao, Antoinette Fong, See Voon Seow, Han Chong Toh
Summary: Landmark discoveries in cell-mediated immunity since the last century have revolutionized the treatment of various diseases, including cancer, through a greater understanding of the immune system. Precision immuno-oncology involves targeting immune checkpoints and harnessing immune cell therapies. The limited efficacy in some cancers is attributed to the complex tumor microenvironment, which comprises various cell types and contributes to immune evasion. Organoids provide a dynamic study of the interactions between tumor cells and the tumor microenvironment, and hold potential in improving precision immuno-oncology.
Review
Cell Biology
Minakshi Prasad, Rajesh Kumar, Lukumoni Buragohain, Ankur Kumari, Mayukh Ghosh
Summary: Engineered nanomaterials possess unique physicochemical properties that make them suitable for diverse biomedical and pharmaceutical applications. Toxicity assessment of these materials is crucial for ensuring safety, and the use of organoid models shows potential advantages in this regard.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Zilong Zhou, Lele Cong, Xianling Cong
Summary: Organoids are three-dimensional cellular structures that resemble organs, can be generated from healthy or pathological tissues, and retain cellular composition and drug features. Patient-derived organoids are used in clinical trials to predict responses to treatment and potentially improve outcomes.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Haotian Chen, Wentao Zhang, Niraj Maskey, Fuhan Yang, Zongtai Zheng, Cheng Li, Ruiliang Wang, Pengfei Wu, Shiyu Mao, Junfeng Zhang, Yang Yan, Wei Li, Xudong Yao
Summary: Urological cancers are common malignant tumors worldwide. Traditional two-dimensional cell cultures and animal models cannot fully simulate the complexity of tumors and their interactions with the surrounding environment. The emergence of three-dimensional organoid models provides a new solution for studying urological cancers but still faces some challenges.
CELL AND BIOSCIENCE
(2022)
Article
Chemistry, Analytical
Miki Matsumoto, Yuya Morimoto, Toshiro Sato, Shoji Takeuchi
Summary: In this study, a microfluidic organoid-trapping device was proposed to immobilize and stimulate human intestinal organoids. The device successfully trapped and cultured human intestinal organoids, and demonstrated the ability to induce organoid fusion and regulate their surface structures using fluidic shear stress.
Review
Engineering, Biomedical
Woochan Kim, Yonghyun Gwon, Sunho Park, Hyoseong Kim, Jangho Kim
Summary: Three-dimensional stem cell culture systems have the potential to mimic complex interactions between cells and the extracellular matrix, and have unique properties that can be applied in therapeutics.
BIOACTIVE MATERIALS
(2023)
Review
Oncology
Ya-ya Yu, Yan-juan Zhu, Zhen-zhen Xiao, Ya-dong Chen, Xue-song Chang, Yi-hong Liu, Qing Tang, Hai-bo Zhang
Summary: Gastrointestinal cancers (GICs) are a significant part of global cancer incidence and mortality, and the long-term overall survival of GIC patients has not been improved despite advances in treatment strategies. Recent research suggests that patient-derived organoid (PDO) culture technology can recapitulate the characteristics of GICs, providing potential for personalized treatment. Establishing PDO biobanks enables the selection of radiotherapy, chemotherapy, targeted therapy, and immunotherapy for GICs.
BIOMARKER RESEARCH
(2022)
Review
Engineering, Biomedical
Preety Rawal, Dinesh M. Tripathi, Seeram Ramakrishna, Savneet Kaur
Summary: Three-dimensional bioprinting technology is emerging to address limitations in current organoid technologies, showing success in printing stem cells and organoids. Vascularization strategies for bioprinted organoids are critical for achieving complex tissue organization. Harnessing and directing advancements in 3D bioprinting is essential for realizing the translational applications of organoids in disease modeling and regenerative medicine.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Chemistry, Multidisciplinary
Stephen M. Jones, David E. Heppner, Kenny Vu, Daniel J. Kosman, Edward Solomon
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Clinical Neurology
Austin T. K. Hoke, Madison Malfitano, Adam M. Zanation, Charles S. Ebert, Brent A. Senior, Adam J. Kimple, Brian D. Thorp
Summary: This study investigates the prescribing and use of opioid analgesics following endoscopic pituitary surgery, and finds a wide variation in prescribing practices among providers and a lack of standardized protocols.
JOURNAL OF NEUROLOGICAL SURGERY PART B-SKULL BASE
(2022)
Article
Oncology
Austin T. K. Hoke, Michelle R. Padget, Kellsye P. Fabian, Anjali Nandal, Gary L. Gallia, Marijo Bilusic, Patrick Soon-Shiong, James W. Hodge, Nyall R. London Jr
Summary: Research has shown that using anti-PD-L1 and anti-EGFR antibodies can enhance NK cell lysis of chordoma cells, and NK cell toxicity against chordoma cells is increased after treatment with IL15 superagonist complex.
CANCER RESEARCH COMMUNICATIONS
(2021)