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Title
Challenges and opportunities in cryo-EM single-particle analysis
Authors
Keywords
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Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 294, Issue 13, Pages 5181-5197
Publisher
American Society for Biochemistry & Molecular Biology (ASBMB)
Online
2019-02-26
DOI
10.1074/jbc.rev118.005602
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Note: Only part of the references are listed.- The energy dependence of contrast and damage in electron cryomicroscopy of biological molecules
- (2019) Mathew J. Peet et al. ULTRAMICROSCOPY
- From Electron Crystallography to Single Particle CryoEM (Nobel Lecture)
- (2018) Richard Henderson ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
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- (2018) Kazuyoshi Murata et al. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
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- (2018) David Haselbach et al. CELL
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- (2018) Robert M. Glaeser CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
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- (2018) Si-Sun Choi et al. CURRENT OPINION IN MICROBIOLOGY
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- (2018) Eugene Palovcak et al. JOURNAL OF STRUCTURAL BIOLOGY
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- (2018) Jean-Paul Renaud et al. NATURE REVIEWS DRUG DISCOVERY
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- (2018) Yuxi Liu et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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- (2018) Jose Luis Vilas et al. STRUCTURE
- Analysis of Global and Site-Specific Radiation Damage in Cryo-EM
- (2018) Johan Hattne et al. STRUCTURE
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- (2018) Alberto Bartesaghi et al. STRUCTURE
- Classification of Single Particles from Human Cell Extract Reveals Distinct Structures
- (2018) Eric J. Verbeke et al. Cell Reports
- Routine single particle CryoEM sample and grid characterization by tomography
- (2018) Alex J Noble et al. eLife
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- (2018) Takanori Nakane et al. eLife
- cisTEM, user-friendly software for single-particle image processing
- (2018) Timothy Grant et al. eLife
- Model validation: local diagnosis, correction and when to quit
- (2018) Jane S. Richardson et al. Acta Crystallographica Section D-Structural Biology
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- (2018) Thomas C. Terwilliger et al. Acta Crystallographica Section D-Structural Biology
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- (2018) Ieva Drulyte et al. Acta Crystallographica Section D-Structural Biology
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- (2018) Robert A. Nicholls et al. Acta Crystallographica Section D-Structural Biology
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- (2018) David Balchin et al. CELL
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- (2018) Anchi Cheng et al. JOURNAL OF STRUCTURAL BIOLOGY
- Structural insights into G-protein-coupled receptor allostery
- (2018) David M. Thal et al. NATURE
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- (2018) Chi-Min Ho et al. NATURE
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- (2018) Giovanna Scapin et al. NATURE
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- (2018) Alex J. Noble et al. NATURE METHODS
- Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
- (2018) Yong Zi Tan et al. Nature Communications
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- (2018) Claudio Schmidli et al. Jove-Journal of Visualized Experiments
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- (2018) Pavel V. Afonine et al. Acta Crystallographica Section D-Structural Biology
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- (2018) Bertrand Beckert et al. Nature Microbiology
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- (2018) Stefano Stella et al. CELL
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- (2018) Stefan Pfeffer et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
- A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps
- (2018) Thomas C. Terwilliger et al. NATURE METHODS
- Structural basis of the nucleosome transition during RNA polymerase II passage
- (2018) Tomoya Kujirai et al. SCIENCE
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- (2018) Avinash B. Patel et al. SCIENCE
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- (2018) Jasenko Zivanov et al. eLife
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- (2018) Maximilian Beckers et al. IUCrJ
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- (2017) Nikhil Biyani et al. JOURNAL OF STRUCTURAL BIOLOGY
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- (2017) Joost Snijder et al. JOURNAL OF STRUCTURAL BIOLOGY
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- (2017) Maryam Khoshouei et al. JOURNAL OF STRUCTURAL BIOLOGY
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- (2017) Joachim Frank JOURNAL OF STRUCTURAL BIOLOGY
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- (2016) Thomas G. Martin et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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- (2016) Jimin Wang PROTEIN SCIENCE
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- (2015) Benjamin A Barad et al. NATURE METHODS
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- (2015) Koji Yonekura et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Structural basis of pre-mRNA splicing
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- (2015) A. Amunts et al. SCIENCE
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- (2015) Shenping Wu et al. Microscopy
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- (2015) Yong Zi Tan et al. Microscopy
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- (2012) Axel F. Brilot et al. JOURNAL OF STRUCTURAL BIOLOGY
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- (2008) X. Zhang et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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