Approaches to altering particle distributions in cryo-electron microscopy sample preparation

Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure

(2018) Mayuriben Parmar et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Optimizing “self-wicking” nanowire grids

(2018) Hui Wei et al.   JOURNAL OF STRUCTURAL BIOLOGY

Spotiton: New features and applications

(2018) Venkata P. Dandey et al.   JOURNAL OF STRUCTURAL BIOLOGY

Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM

(2018) Shaun Rawson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Deactive State of Mammalian Respiratory Complex I

(2018) James N. Blaza et al.   STRUCTURE

The Structure of an Infectious Human Polyomavirus and Its Interactions with Cellular Receptors

(2018) Daniel L. Hurdiss et al.   STRUCTURE

Large-scale two-photon imaging revealed super-sparse population codes in the V1 superficial layer of awake monkeys

(2018) Shiming Tang et al.   eLife

X-ray and cryo-EM structures of inhibitor-bound cytochrome bc 1 complexes for structure-based drug discovery

(2018) Kangsa Amporndanai et al.   IUCrJ

Revisiting the Structure of Hemoglobin and Myoglobin with Cryo-Electron Microscopy

(2017) Maryam Khoshouei et al.   JOURNAL OF MOLECULAR BIOLOGY

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

(2017) Stefan A. Arnold et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the Insulin Receptor in Complex with Insulin using Single Particle CryoEM Analysis

(2017) Giovanna Scapin et al.   MICROSCOPY AND MICROANALYSIS

Cryo-EM structures of tau filaments from Alzheimer’s disease

(2017) Anthony W. P. Fitzpatrick et al.   NATURE

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Role of enhanced receptor engagement in the evolution of a pandemic acute hemorrhagic conjunctivitis virus

(2017) Jim Baggen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM

(2017) Xiangsong Feng et al.   STRUCTURE

Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex

(2017) Jin Young Kang et al.   eLife

The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide

(2017) Qing Xie et al.   Molecular Therapy-Methods & Clinical Development

Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens

(2016) Robert M. Glaeser et al.   BIOPHYSICAL JOURNAL

Mass spectrometry guided structural biology

(2016) Idlir Liko et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A new method for vitrifying samples for cryoEM

(2016) Ivan Razinkov et al.   JOURNAL OF STRUCTURAL BIOLOGY

Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates

(2016) Christopher J. Benjamin et al.   LANGMUIR

Methods to account for movement and flexibility in cryo-EM data processing

(2016) S. Rawson et al.   METHODS

Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION

(2016) Tanmay A M Bharat et al.   Nature Protocols

Design of a molecular support for cryo-EM structure determination

(2016) Thomas G. Martin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution

(2016) Guimei Yu et al.   STRUCTURE

RNA polymerase I–Rrn3 complex at 4.8 Å resolution

(2016) Christoph Engel et al.   Nature Communications

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

(2015) Thi Hoang Duong Nguyen et al.   NATURE

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

(2015) Ashwin Chari et al.   NATURE METHODS

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM

(2015) Florian Hauer et al.   STRUCTURE

Structure of the Vacuolar H + -ATPase Rotary Motor Reveals New Mechanistic Insights

(2015) Shaun Rawson et al.   STRUCTURE

Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM

(2015) Emma L. Hesketh et al.   Nature Communications

Cryo-EM reveals the conformation of a substrate analogue in the human 20S proteasome core

(2015) Paula C.A. da Fonseca et al.   Nature Communications

Rendering graphene supports hydrophilic with non-covalent aromatic functionalization for transmission electron microscopy

(2014) Radosav S. Pantelic et al.   APPLIED PHYSICS LETTERS

PA1b Inhibitor Binding to Subunitscandeof the Vacuolar ATPase Reveals Its Insecticidal Mechanism

(2014) Stephen P. Muench et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies

(2014) Guimei Yu et al.   JOURNAL OF STRUCTURAL BIOLOGY

Chemically functionalized carbon films for single molecule imaging

(2014) Marc C. Llaguno et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of a type IV secretion system

(2014) Harry H. Low et al.   NATURE

Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter

(2014) JungMin Kim et al.   NATURE

Structure of the AcrAB–TolC multidrug efflux pump

(2014) Dijun Du et al.   NATURE

Architecture of mammalian respiratory complex I

(2014) Kutti R. Vinothkumar et al.   NATURE

Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas

(2014) Christopher J Russo et al.   NATURE METHODS

Ultrastable gold substrates for electron cryomicroscopy

(2014) C. J. Russo et al.   SCIENCE

Visualizing active membrane protein complexes by electron cryotomography

(2014) Vicki A.M. Gold et al.   Nature Communications

Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

(2014) Joel R. Meyerson et al.   Scientific Reports

Beam-induced motion correction for sub-megadalton cryo-EM particles

(2014) Sjors HW Scheres   eLife

Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1

(2013) Vu Q. Nguyen et al.   CELL

Fabrication of carbon films with ∼500nm holes for cryo-EM with a direct detector device

(2013) Chelsea R. Marr et al.   JOURNAL OF STRUCTURAL BIOLOGY

Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM

(2012) Yi-Min Wu et al.   EMBO JOURNAL

Beam-induced motion of vitrified specimen on holey carbon film

(2012) Axel F. Brilot et al.   JOURNAL OF STRUCTURAL BIOLOGY

Spotiton: A prototype for an integrated inkjet dispense and vitrification system for cryo-TEM

(2012) Tilak Jain et al.   JOURNAL OF STRUCTURAL BIOLOGY

ATP-Driven Remodeling of the Linker Domain in the Dynein Motor

(2012) Anthony J. Roberts et al.   STRUCTURE

Radiation damage in single-particle cryo-electron microscopy: effects of dose and dose rate

(2011) Manikandan Karuppasamy et al.   JOURNAL OF SYNCHROTRON RADIATION

Cryomesh™: A New Substrate for Cryo-Electron Microscopy

(2010) Craig Yoshioka et al.   MICROSCOPY AND MICROANALYSIS

Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label

(2010) Dirk Flemming et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural organisation of phycobilisomes from Synechocystis sp. strain PCC6803 and their interaction with the membrane

(2009) Ana A. Arteni et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Graphene oxide: A substrate for optimizing preparations of frozen-hydrated samples

(2009) Radosav S. Pantelic et al.   JOURNAL OF STRUCTURAL BIOLOGY

Adeno-associated virus-2 and its primary cellular receptor—Cryo-EM structure of a heparin complex

(2009) Jason O'Donnell et al.   VIROLOGY

Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on opportunities for the future

(2008) Kenneth A. Taylor et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron cryo-microscopy of biological specimens on conductive titanium–silicon metal glass films

(2007) Daniel Rhinow et al.   ULTRAMICROSCOPY