Cryo-EM in drug discovery: achievements, limitations and prospects

The 3.5-Å CryoEM Structure of Nanodisc-Reconstituted Yeast Vacuolar ATPase V o Proton Channel

(2018) Soung-Hun Roh et al.   MOLECULAR CELL

Structural insights into the voltage and phospholipid activation of the mammalian TPC1 channel

(2018) Ji She et al.   NATURE

Modular assembly of the nucleolar pre-60S ribosomal subunit

(2018) Zahra Assur Sanghai et al.   NATURE

Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å

(2018) Pu Qian et al.   NATURE

Architecture of the human GATOR1 and GATOR1–Rag GTPases complexes

(2018) Kuang Shen et al.   NATURE

Structure of the alternative complex III in a supercomplex with cytochrome oxidase

(2018) Chang Sun et al.   NATURE

Structural basis of small-molecule inhibition of human multidrug transporter ABCG2

(2018) Scott M. Jackson et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex

(2018) Marcello Clerici et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms

(2018) Zengqin Deng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes

(2018) Simon Poepsel et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM

(2018) Taylor E. T. Hughes et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system

(2018) Yuxi Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Binding of ISRIB reveals a regulatory site in the nucleotide exchange factor eIF2B

(2018) Alisa F. Zyryanova et al.   SCIENCE

Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule

(2018) Jordan C. Tsai et al.   SCIENCE

Cryo-EM structure of a herpesvirus capsid at 3.1 Å

(2018) Shuai Yuan et al.   SCIENCE

Cryo-EM structure of 5-HT3A receptor in its resting conformation

(2018) Sandip Basak et al.   Nature Communications

Structural basis of small-molecule inhibition of human multidrug transporter ABCG2

(2018) Scott M. Jackson et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex

(2018) Marcello Clerici et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms

(2018) Zengqin Deng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes

(2018) Simon Poepsel et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM

(2018) Taylor E. T. Hughes et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

cisTEM, user-friendly software for single-particle image processing

(2018) Timothy Grant et al.   eLife

Cryo-EM structure of the gasdermin A3 membrane pore

(2018) Jianbin Ruan et al.   NATURE

Structure of the insulin receptor–insulin complex by single-particle cryo-EM analysis

(2018) Giovanna Scapin et al.   NATURE

Cryo-EM shows how dynactin recruits two dyneins for faster movement

(2018) Linas Urnavicius et al.   NATURE

The atomic structure of a eukaryotic oligosaccharyltransferase complex

(2018) Lin Bai et al.   NATURE

Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor–Gs complex

(2018) Yi-Lynn Liang et al.   NATURE

Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures

(2017) Elizabeth H. Kellogg et al.   JOURNAL OF MOLECULAR BIOLOGY

Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids

(2017) Joost Snijder et al.   JOURNAL OF STRUCTURAL BIOLOGY

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

(2017) Stefan A. Arnold et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure and Dynamics of a 197 bp Nucleosome in Complex with Linker Histone H1

(2017) Jan Bednar et al.   MOLECULAR CELL

Human TRPML1 channel structures in open and closed conformations

(2017) Philip Schmiege et al.   NATURE

Phase-plate cryo-EM structure of a class B GPCR–G-protein complex

(2017) Yi-Lynn Liang et al.   NATURE

Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3

(2017) Marscha Hirschi et al.   NATURE

Structure of mammalian endolysosomal TRPML1 channel in nanodiscs

(2017) Qingfeng Chen et al.   NATURE

Structure of a eukaryotic cyclic-nucleotide-gated channel

(2017) Minghui Li et al.   NATURE

Structure of a spliceosome remodelled for exon ligation

(2017) Sebastian M. Fica et al.   NATURE

Cryo-EM structure of a human spliceosome activated for step 2 of splicing

(2017) Karl Bertram et al.   NATURE

Mediator structure and rearrangements required for holoenzyme formation

(2017) Kuang-Lei Tsai et al.   NATURE

Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein

(2017) Yan Zhang et al.   NATURE

Cryo-EM structures of tau filaments from Alzheimer’s disease

(2017) Anthony W. P. Fitzpatrick et al.   NATURE

Correcting for cell-type heterogeneity in epigenome-wide association studies: revisiting previous analyses

(2017) Shijie C Zheng et al.   NATURE METHODS

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

(2017) Mark A Herzik et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Advances in the field of single-particle cryo-electron microscopy over the last decade

(2017) Joachim Frank   Nature Protocols

Structures of the human mitochondrial ribosome in native states of assembly

(2017) Alan Brown et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage

(2017) Jingdong Cheng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The complete structure of the small-subunit processome

(2017) Jonas Barandun et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer

(2017) Qingbo Liu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Antibody-induced uncoating of human rhinovirus B14

(2017) Yangchao Dong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-particle cryo-EM-Improved ab initio 3D reconstruction with SIMPLE/PRIME

(2017) Cyril F. Reboul et al.   PROTEIN SCIENCE

Architecture of eukaryotic mRNA 3′-end processing machinery

(2017) Ana Casañal et al.   SCIENCE

Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome

(2017) Dario Oliveira Passos et al.   SCIENCE

Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection

(2017) Max E. Wilkinson et al.   SCIENCE

Structure of a eukaryotic voltage-gated sodium channel at near-atomic resolution

(2017) Huaizong Shen et al.   SCIENCE

Cryo-EM structures of the triheteromeric NMDA receptor and its allosteric modulation

(2017) Wei Lü et al.   SCIENCE

Structure of the yeast spliceosomal postcatalytic P complex

(2017) Shiheng Liu et al.   SCIENCE

Fibril structure of amyloid-β(1–42) by cryo–electron microscopy

(2017) Lothar Gremer et al.   SCIENCE

A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM

(2017) Xiangsong Feng et al.   STRUCTURE

Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin

(2017) Moran Shalev-Benami et al.   Nature Communications

Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

(2017) Maryam Khoshouei et al.   Nature Communications

Structures of the human mitochondrial ribosome in native states of assembly

(2017) Alan Brown et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage

(2017) Jingdong Cheng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The complete structure of the small-subunit processome

(2017) Jonas Barandun et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer

(2017) Qingbo Liu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

EMBuilder: A Template Matching-based Automatic Model-building Program for High-resolution Cryo-Electron Microscopy Maps

(2017) Niyun Zhou et al.   Scientific Reports

Using the Volta phase plate with defocus for cryo-EM single particle analysis

(2017) Radostin Danev et al.   eLife

Model-based local density sharpening of cryo-EM maps

(2017) Arjen J Jakobi et al.   eLife

The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide

(2017) Qing Xie et al.   Molecular Therapy-Methods & Clinical Development

Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis

(2017) Wilson Wong et al.   Nature Microbiology

Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens

(2016) Robert M. Glaeser et al.   BIOPHYSICAL JOURNAL

Structural Basis for Gating and Activation of RyR1

(2016) Amédée des Georges et al.   CELL

Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating

(2016) Doreen Matthies et al.   CELL

Atomic Structure of the Cystic Fibrosis Transmembrane Conductance Regulator

(2016) Zhe Zhang et al.   CELL

The Structure of the Polycystic Kidney Disease Channel PKD2 in Lipid Nanodiscs

(2016) Peter S. Shen et al.   CELL

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

Structure of Mammalian Respiratory Supercomplex I 1 III 2 IV 1

(2016) Meng Wu et al.   CELL

Progress towards an optimal specimen support for electron cryomicroscopy

(2016) Christopher J Russo et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A new method for vitrifying samples for cryoEM

(2016) Ivan Razinkov et al.   JOURNAL OF STRUCTURAL BIOLOGY

Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates

(2016) Christopher J. Benjamin et al.   LANGMUIR

Antibody-based affinity cryo-EM grid

(2016) Guimei Yu et al.   METHODS

High resolution single particle refinement in EMAN2.1

(2016) James M. Bell et al.   METHODS

An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

(2016) Rebecca F. Thompson et al.   METHODS

A mechanism of viral immune evasion revealed by cryo-EM analysis of the TAP transporter

(2016) Michael L. Oldham et al.   NATURE

Structural basis of kainate subtype glutamate receptor desensitization

(2016) Joel R. Meyerson et al.   NATURE

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

Structure of the adenosine A2A receptor bound to an engineered G protein

(2016) Byron Carpenter et al.   NATURE

The pathway to GTPase activation of elongation factor SelB on the ribosome

(2016) Niels Fischer et al.   NATURE

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution

(2016) Thi Hoang Duong Nguyen et al.   NATURE

Cryo-EM structure of the spliceosome immediately after branching

(2016) Wojciech P. Galej et al.   NATURE

Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function

(2016) Allison Ballandras-Colas et al.   NATURE

Near-atomic resolution visualization of human transcription promoter opening

(2016) Yuan He et al.   NATURE

Transcription initiation complex structures elucidate DNA opening

(2016) C. Plaschka et al.   NATURE

Structure of the voltage-gated calcium channel Cav1.1 at 3.6 Å resolution

(2016) Jianping Wu et al.   NATURE

Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution

(2016) Julian von der Ecken et al.   NATURE

Cryo-EM structure of the open high-conductance Ca2+-activated K+ channel

(2016) Xiao Tao et al.   NATURE

The democratization of cryo-EM

(2016) David I Stuart et al.   NATURE METHODS

Twenty years on: the impact of fragments on drug discovery

(2016) Daniel A. Erlanson et al.   NATURE REVIEWS DRUG DISCOVERY

A comprehensive map of molecular drug targets

(2016) Rita Santos et al.   NATURE REVIEWS DRUG DISCOVERY

Cryo-electron microscopy structure of the TRPV2 ion channel

(2016) Lejla Zubcevic et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site

(2016) Harry B Gristick et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM

(2016) Eugene Y.D. Chua et al.   NUCLEIC ACIDS RESEARCH

Structure and assembly model for theTrypanosoma cruzi60S ribosomal subunit

(2016) Zheng Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of an endogenous yeast 26S proteasome reveals two major conformational states

(2016) Bai Luan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Determination of the ribosome structure to a resolution of 2.5 Å by single-particle cryo-EM

(2016) Zheng Liu et al.   PROTEIN SCIENCE

Molecular architecture of the human U4/U6.U5 tri-snRNP

(2016) D. E. Agafonov et al.   SCIENCE

Structure of a yeast catalytic step I spliceosome at 3.4 A resolution

(2016) R. Wan et al.   SCIENCE

Structure of a yeast step II catalytically activated spliceosome

(2016) Chuangye Yan et al.   SCIENCE

Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer

(2016) J. H. Lee et al.   SCIENCE

Structure of the voltage-gated K+ channel Eag1 reveals an alternative voltage sensing mechanism

(2016) J. R. Whicher et al.   SCIENCE

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome

(2016) R. Rauhut et al.   SCIENCE

Structure of a yeast activated spliceosome at 3.5 A resolution

(2016) C. Yan et al.   SCIENCE

2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

(2016) S. Banerjee et al.   SCIENCE

The 3.8 A resolution cryo-EM structure of Zika virus

(2016) D. Sirohi et al.   SCIENCE

Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution

(2016) Guimei Yu et al.   STRUCTURE

Volta phase plate cryo-EM of the small protein complex Prx3

(2016) Maryam Khoshouei et al.   Nature Communications

Cryo-electron microscopy structure of the TRPV2 ion channel

(2016) Lejla Zubcevic et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site

(2016) Harry B Gristick et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM grids Modified with NTA-Graphene Oxide

(2016) Christopher J. Benjamin et al.   Scientific Reports

Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps

(2016) Abhishek Singharoy et al.   eLife

Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition

(2016) Corey M Dambacher et al.   eLife

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

The trimeric serine protease HtrA1 forms a cage-like inhibition complex with an anti-HtrA1 antibody

(2015) C. Ciferri et al.   BIOCHEMICAL JOURNAL

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins

(2015) Rui Zhang et al.   CELL

X-ray crystallography over the past decade for novel drug discovery – where are we heading next?

(2015) Heping Zheng et al.   Expert Opinion on Drug Discovery

FEI’s direct electron detector developments: Embarking on a revolution in cryo-TEM

(2015) Maarten Kuijper et al.   JOURNAL OF STRUCTURAL BIOLOGY

Automatic estimation and correction of anisotropic magnification distortion in electron microscopes

(2015) Timothy Grant et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the TRPA1 ion channel suggests regulatory mechanisms

(2015) Candice E. Paulsen et al.   NATURE

Structure of the E. coli ribosome–EF-Tu complex at

(2015) Niels Fischer et al.   NATURE

Atomic structure of anthrax protective antigen pore elucidates toxin translocation

(2015) Jiansen Jiang et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

Glycine receptor mechanism elucidated by electron cryo-microscopy

(2015) Juan Du et al.   NATURE

Architecture of the mammalian mechanosensitive Piezo1 channel

(2015) Jingpeng Ge et al.   NATURE

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

(2015) Ashwin Chari et al.   NATURE METHODS

De novo protein structure determination from near-atomic-resolution cryo-EM maps

(2015) Ray Yu-Ruei Wang et al.   NATURE METHODS

Cryo-EM structures elucidate neutralizing mechanisms of anti-chikungunya human monoclonal antibodies with therapeutic activity

(2015) Feng Long et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

2.2 A resolution cryo-EM structure of  -galactosidase in complex with a cell-permeant inhibitor

(2015) A. Bartesaghi et al.   SCIENCE

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM

(2015) Florian Hauer et al.   STRUCTURE

Structural Dynamics of Ribosome Subunit Association Studied by Mixing-Spraying Time-Resolved Cryogenic Electron Microscopy

(2015) Bo Chen et al.   STRUCTURE

How cryo-EM is revolutionizing structural biology

(2015) Xiao-chen Bai et al.   TRENDS IN BIOCHEMICAL SCIENCES

Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

(2015) Timothy Grant et al.   eLife

Sampling the conformational space of the catalytic subunit of human γ-secretase

(2015) Xiao-chen Bai et al.   eLife

Collaborative Computational Project for Electron cryo-Microscopy

(2015) Chris Wood et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Robust evaluation of 3D electron cryomicroscopy data using tilt-pairs

(2014) Christopher J. Russo et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution

(2014) Zhen Yan et al.   NATURE

Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas

(2014) Christopher J Russo et al.   NATURE METHODS

Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM

(2014) Tanvir R. Shaikh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome

(2014) P. Unverdorben et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Yeast Mitochondrial Large Ribosomal Subunit

(2014) A. Amunts et al.   SCIENCE

Ultrastable gold substrates for electron cryomicroscopy

(2014) C. J. Russo et al.   SCIENCE

The Resolution Revolution

(2014) W. Kuhlbrandt   SCIENCE

Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy

(2014) G. McMullan et al.   ULTRAMICROSCOPY

Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

(2014) Matteo Allegretti et al.   eLife

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

(2014) Wilson Wong et al.   eLife

Likelihood-based classification of cryo-EM images using FREALIGN

(2013) Dmitry Lyumkis et al.   JOURNAL OF STRUCTURAL BIOLOGY

Maximizing the potential of electron cryomicroscopy data collected using direct detectors

(2013) David Veesler et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging

(2013) Kasim Sader et al.   JOURNAL OF STRUCTURAL BIOLOGY

TRPV1 structures in distinct conformations reveal activation mechanisms

(2013) Erhu Cao et al.   NATURE

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Engineered nanostructured β-sheet peptides protect membrane proteins

(2013) Houchao Tao et al.   NATURE METHODS

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Optimization of protein purification and characterization using Thermofluor screens

(2013) Stephane Boivin et al.   PROTEIN EXPRESSION AND PURIFICATION

Molecular Architecture of a Eukaryotic Translational Initiation Complex

(2013) I. S. Fernandez et al.   SCIENCE

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

(2013) Xiao-chen Bai et al.   eLife

Beam-induced motion of vitrified specimen on holey carbon film

(2012) Axel F. Brilot et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Vemurafenib: the first drug approved for BRAF-mutant cancer

(2012) Gideon Bollag et al.   NATURE REVIEWS DRUG DISCOVERY

Near-atomic resolution structural model of the yeast 26S proteasome

(2012) F. Beck et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Linking Crystallographic Model and Data Quality

(2012) P. A. Karplus et al.   SCIENCE

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

Fabs Enable Single Particle cryoEM Studies of Small Proteins

(2012) Shenping Wu et al.   STRUCTURE

Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects

(2011) Robert M. Glaeser et al.   BIOPHYSICAL JOURNAL

Structural Analysis of Macromolecular Assemblies by Electron Microscopy

(2011) E. V. Orlova et al.   CHEMICAL REVIEWS

Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy

(2011) Anna-Clare Milazzo et al.   JOURNAL OF STRUCTURAL BIOLOGY

Computer controlled cryo-electron microscopy – TOM2 a software package for high-throughput applications

(2011) Andreas Korinek et al.   JOURNAL OF STRUCTURAL BIOLOGY

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

The Structure of the Eukaryotic Ribosome at 3.0 A Resolution

(2011) A. Ben-Shem et al.   SCIENCE

An adaptive Expectation–Maximization algorithm with GPU implementation for electron cryomicroscopy

(2010) Hemant D. Tagare et al.   JOURNAL OF STRUCTURAL BIOLOGY

Automated screening of 2D crystallization trials using transmission electron microscopy: A high-throughput tool-chain for sample preparation and microscopic analysis

(2010) Nicolas Coudray et al.   JOURNAL OF STRUCTURAL BIOLOGY

An Approach for De Novo Structure Determination of Dynamic Molecular Assemblies by Electron Cryomicroscopy

(2010) Bjoern Sander et al.   STRUCTURE

Snapshots of the RNA editing machine in trypanosomes captured at different assembly stages in vivo

(2009) Monika M Golas et al.   EMBO JOURNAL

Monolithic microfluidic mixing–spraying devices for time-resolved cryo-electron microscopy

(2009) Zonghuan Lu et al.   JOURNAL OF STRUCTURAL BIOLOGY

Appion: An integrated, database-driven pipeline to facilitate EM image processing

(2009) Gabriel C. Lander et al.   JOURNAL OF STRUCTURAL BIOLOGY

Graphene oxide: A substrate for optimizing preparations of frozen-hydrated samples

(2009) Radosav S. Pantelic et al.   JOURNAL OF STRUCTURAL BIOLOGY

New controlled environment vitrification system for preparing wet samples for cryo-SEM

(2008) H. GE et al.   JOURNAL OF MICROSCOPY

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

(2008) Xuekui Yu et al.   NATURE

Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy

(2008) Wen Jiang et al.   NATURE

Perspectives on NMR in drug discovery: a technique comes of age

(2008) Maurizio Pellecchia et al.   NATURE REVIEWS DRUG DISCOVERY

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction

(2008) X. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De Novo Backbone Trace of GroEL from Single Particle Electron Cryomicroscopy

(2008) Steven J. Ludtke et al.   STRUCTURE

Computational resources for cryo-electron tomography in Bsoft

(2007) J. Bernard Heymann et al.   JOURNAL OF STRUCTURAL BIOLOGY