New tools for automated high-resolution cryo-EM structure determination in RELION-3

Atomic Resolution Cryo-EM Structure of β-Galactosidase

(2018) Alberto Bartesaghi et al.   STRUCTURE

Ewald sphere correction using a single side-band image processing algorithm

(2018) Christopher J. Russo et al.   ULTRAMICROSCOPY

Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

(2018) Dongjie Zhu et al.   Nature Communications

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

(2018) Takanori Nakane et al.   eLife

cisTEM, user-friendly software for single-particle image processing

(2018) Timothy Grant et al.   eLife

Cisplatin Binding Sites in Human H-Chain Ferritin

(2017) Giarita Ferraro et al.   INORGANIC CHEMISTRY

Focus: The interface between data collection and data processing in cryo-EM

(2017) Nikhil Biyani et al.   JOURNAL OF STRUCTURAL BIOLOGY

Robust image alignment for cryogenic transmission electron microscopy

(2017) Robert A. McLeod et al.   JOURNAL OF STRUCTURAL BIOLOGY

Helical reconstruction in RELION

(2017) Shaoda He et al.   JOURNAL OF STRUCTURAL BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Accurate model annotation of a near-atomic resolution cryo-EM map

(2017) Corey F. Hryc et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy

(2017) Katerina Naydenova et al.   Nature Communications

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

(2017) Toshio Moriya et al.   Jove-Journal of Visualized Experiments

Trends in the Electron Microscopy Data Bank (EMDB)

(2017) Ardan Patwardhan   Acta Crystallographica Section D-Structural Biology

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy

(2016) J.M. de la Rosa-Trevín et al.   JOURNAL OF STRUCTURAL BIOLOGY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Unravelling biological macromolecules with cryo-electron microscopy

(2016) Rafael Fernandez-Leiro et al.   NATURE

EMPIAR: a public archive for raw electron microscopy image data

(2016) Andrii Iudin et al.   NATURE METHODS

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

Alignment of direct detection device micrographs using a robust Optical Flow approach

(2015) Vahid Abrishami et al.   JOURNAL OF STRUCTURAL BIOLOGY

Alignment of cryo-EM movies of individual particles by optimization of image translations

(2015) John L. Rubinstein et al.   JOURNAL OF STRUCTURAL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Semi-automated selection of cryo-EM particles in RELION-1.3

(2015) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

2.2 A resolution cryo-EM structure of  -galactosidase in complex with a cell-permeant inhibitor

(2015) A. Bartesaghi et al.   SCIENCE

Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging

(2015) Tanmay A.M. Bharat et al.   STRUCTURE

Sampling the conformational space of the catalytic subunit of human γ-secretase

(2015) Xiao-chen Bai et al.   eLife

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

(2015) Alan Brown et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The Resolution Revolution

(2014) W. Kuhlbrandt   SCIENCE

Quantifying the local resolution of cryo-EM density maps

(2013) Alp Kucukelbir et al.   NATURE METHODS

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Structural analysis of haemin demetallation by L-chain apoferritins

(2012) Natalia de Val et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Beam-induced motion of vitrified specimen on holey carbon film

(2012) Axel F. Brilot et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Cross-neutralization of influenza A viruses mediated by a single antibody loop

(2012) Damian C. Ekiert et al.   NATURE

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM

(2010) Robert M. Glaeser et al.   JOURNAL OF STRUCTURAL BIOLOGY

DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy

(2009) N.R. Voss et al.   JOURNAL OF STRUCTURAL BIOLOGY

Detective quantum efficiency of electron area detectors in electron microscopy

(2009) G. McMullan et al.   ULTRAMICROSCOPY

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

(2008) Xuekui Yu et al.   NATURE

Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy

(2008) Wen Jiang et al.   NATURE

Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction

(2008) X. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA