Journal
TRENDS IN BIOCHEMICAL SCIENCES
Volume 44, Issue 10, Pages 837-848Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibs.2019.04.008
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Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [18H06043]
- Japan Science and Technology Agency (JST) PRESTO [18069571]
- JSPS KAKENHI [16H02502]
- JST CREST [JPMJCR14M1]
- Platform Project for Supporting Drug Discovery and Life Science Research from AMED [JP19am0101115j003]
- Grants-in-Aid for Scientific Research [18H06043, 16H02502] Funding Source: KAKEN
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Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination technique. Its most prolific branch is single particle analysis (SPA), a method being used in a growing number of laboratories worldwide to determine high-resolution protein structures. Cryo-electron tomography (cryo-ET) is another powerful approach that enables visualization of protein complexes in their native cellular environment. Despite the wide-ranging success of cryo-EM, there are many methodological aspects that could be improved. Those include sample preparation, sample screening, data acquisition, image processing, and structure validation. Future developments will increase the reliability and throughput of the technique and reduce the cost and skill level barrier for its adoption.
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