Monitoring and control of E. coli cell integrity

Comparison of Modeling Methods for DoE‐Based Holistic Upstream Process Characterization

(2020) Benjamin Bayer et al.   Biotechnology Journal

Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

(2020) Artur Schuller et al.   Microbial Cell Factories

High-level extracellular production of an alkaline pectate lyase in E. coli BL21 (DE3) and its application in bioscouring of cotton fabric

(2020) Jie Zhen et al.   3 Biotech

Application of dielectric spectroscopy to unravel the physiological state of microorganisms: current state, prospects and limits

(2020) G. Flores-Cosío et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Monitoring yeast fermentations by nonlinear infrared technology and chemometrics—understanding process correlations and indirect predictions

(2020) Katrin Pontius et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant protein production associated growth inhibition results mainly from transcription and not from translation

(2020) Zhaopeng Li et al.   Microbial Cell Factories

Bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli

(2020) Patrick Stargardt et al.   ACS Synthetic Biology

Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring

(2020) Lorenz Theuer et al.   Processes

A Chemometric Tool to Monitor and Predict Cell Viability in Filamentous Fungi Bioprocesses Using UV Chromatogram Fingerprints

(2020) Philipp Doppler et al.   Processes

Online/At‐line Measurement, Analysis and Control of Product Titer & Critical Product Quality Attributes (CQAs) During Process Development

(2020) Letha Chemmalil et al.   BIOTECHNOLOGY AND BIOENGINEERING

Hybrid Modeling and Intensified DoE: An Approach to Accelerate Upstream Process Characterization

(2020) Benjamin Bayer et al.   Biotechnology Journal

Electrochemical impedimetric biosensors for food safety

(2020) Changhoon Chai et al.   FOOD SCIENCE AND BIOTECHNOLOGY

Combined Optimization of Codon Usage and Glycine Supplementation Enhances the Extracellular Production of a β-Cyclodextrin Glycosyltransferase from Bacillus sp. NR5 UPM in Escherichia coli

(2020) Nik Ida Mardiana Nik-Pa et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Development of a generic reversed-phase liquid chromatography method for protein quantification using analytical quality-by-design principles

(2020) Julian Kopp et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Immobilized Enzymes in Biosensor Applications

(2019) Hoang Nguyen et al.   Materials

SPRi-based hemagglutinin quantitative assay for influenza vaccine production monitoring

(2019) Laurent Durous et al.   VACCINE

Enhancing extracellular protein production in Escherichia coli by deleting the d -alanyl-d -alanine carboxypeptidase gene dacC

(2019) Jinyuan Hu et al.   ENGINEERING IN LIFE SCIENCES

Case study: The characterization and implementation of dielectric spectroscopy (biocapacitance) for process control in a commercial GMP CHO manufacturing process

(2019) Brandon Moore et al.   BIOTECHNOLOGY PROGRESS

Application of the Bradford Assay for Cell Lysis Quantification: Residual Protein Content in Cell Culture Supernatants

(2019) Bernhard Sissolak et al.   Biotechnology Journal

Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram‐negative bacteria

(2019) Hosni Sassi et al.   Microbial Biotechnology

Control of specific growth rate for the enhanced production of human interferon α2b in glycoengineered Pichia pastoris : process analytical technology guided approach

(2019) Srikanth Katla et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

New tools for recombinant protein production in Escherichia coli : A 5-year update

(2019) Germán L. Rosano et al.   PROTEIN SCIENCE

Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

(2019) Alexandros Karyolaimos et al.   Frontiers in Microbiology

Improving production of N-glycosylated recombinant proteins by leaky Escherichia coli

(2019) Ning Ding et al.   3 Biotech

Comparison of Raman and Mid-Infrared Spectroscopy for Real-Time Monitoring of Yeast Fermentations: A Proof-of-Concept for Multi-Channel Photometric Sensors

(2019) Robert Schalk et al.   Applied Sciences-Basel

Monitoring online biomass with a capacitance sensor during scale-up of industrially relevant CHO cell culture fed-batch processes in single-use bioreactors

(2019) S. Metze et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

A bootstrap-aggregated hybrid semi-parametric modeling framework for bioprocess development

(2019) José Pinto et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Bioprocessing in the Digital Age: The Role of Process Models

(2019) Harini Narayanan et al.   Biotechnology Journal

Contemporary Tools for Regulating Gene Expression in Bacteria

(2019) Ross Kent et al.   TRENDS IN BIOTECHNOLOGY

The Rocky Road From Fed-Batch to Continuous Processing With E. coli

(2019) Julian Kopp et al.   Frontiers in Bioengineering and Biotechnology

Probeless non-invasive near-infrared spectroscopic bioprocess monitoring using microspectrometer technology

(2019) Robert Zimmerleiter et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Application of Raman spectroscopy in monoclonal antibody producing continuous systems for downstream process intensification

(2019) Denizhan Yilmaz et al.   BIOTECHNOLOGY PROGRESS

Synergistic inactivation of Escherichia coli O157:H7 by plasma-activated water and mild heat

(2019) Qisen Xiang et al.   FOOD CONTROL

A family of Type VI secretion system effector proteins that form ion-selective pores

(2019) Giuseppina Mariano et al.   Nature Communications

High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations

(2018) Mohammad Russel et al.   ANALYTICA CHIMICA ACTA

Parallel comparison of in situ Raman and NIR spectroscopies to simultaneously measure multiple variables toward real-time monitoring of CHO cell bioreactor cultures

(2018) Mengyao Li et al.   BIOCHEMICAL ENGINEERING JOURNAL

A soft sensor based on online biomass measurements for the glucose estimation and control of fed-batch cultures of Bacillus thuringiensis

(2018) Abdi Escalante-Sánchez et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Secretion of recombinant proteins from E. coli

(2018) Gabriele R. M. Kleiner-Grote et al.   ENGINEERING IN LIFE SCIENCES

Engineering Extracellular Expression Systems in Escherichia coli Based on Transcriptome Analysis and Cell Growth State

(2018) Wen Gao et al.   ACS Synthetic Biology

Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress

(2018) Thomas Baumgarten et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Boosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall Perturbation

(2018) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improvement of extracellular secretion efficiency of Bacillus naganoensis pullulanase from recombinant Escherichia coli: Peptide fusion and cell wall modification

(2018) Xinye Wang et al.   PROTEIN EXPRESSION AND PURIFICATION

Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure

(2018) Haiquan Yang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli

(2018) Lei Wang et al.   BMC BIOTECHNOLOGY

Extracellular production of functional single-chain variable fragment against aflatoxin B1 using Escherichia coli

(2018) K.-I. Na et al.   LETTERS IN APPLIED MICROBIOLOGY

Developing Gram-negative bacteria for the secretion of heterologous proteins

(2018) Lisa Ann Burdette et al.   Microbial Cell Factories

Tuning recombinant protein expression to match secretion capacity

(2018) Luminita Gabriela Horga et al.   Microbial Cell Factories

Simple monitoring of cell leakiness and viability in Escherichia coli bioprocesses-A case study

(2017) David J. Wurm et al.   ENGINEERING IN LIFE SCIENCES

A review of control strategies for manipulating the feed rate in fed-batch fermentation processes

(2017) Lisa Mears et al.   JOURNAL OF BIOTECHNOLOGY

Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions

(2017) Anja Lemoine et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced extracellular production of recombinant proteins in Escherichia coli by co-expression with Bacillus cereus phospholipase C

(2017) Lingqia Su et al.   Microbial Cell Factories

Model-Based Methods in the Biopharmaceutical Process Lifecycle

(2017) Paul Kroll et al.   PHARMACEUTICAL RESEARCH

Mechanistic Fermentation Models for Process Design, Monitoring, and Control

(2017) Lisa Mears et al.   TRENDS IN BIOTECHNOLOGY

Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains

(2017) David J. Wurm et al.   Scientific Reports

Tailoring Escherichia coli for the l-Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins

(2017) Anna Hjelm et al.   ACS Synthetic Biology

Quality by control: Towards model predictive control of mammalian cell culture bioprocesses

(2017) Wolfgang Sommeregger et al.   Biotechnology Journal

A novel toolbox for E. coli lysis monitoring

(2016) Vignesh Rajamanickam et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Raman spectroscopy as a process analytical technology for pharmaceutical manufacturing and bioprocessing

(2016) Karen A. Esmonde-White et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy

(2016) Jiao Li et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints

(2016) Lukas Marschall et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparison of Fiber Optic and Conduit Attenuated Total Reflection (ATR) Fourier Transform Infrared (FT-IR) Setup for In-Line Fermentation Monitoring

(2016) Cosima Koch et al.   APPLIED SPECTROSCOPY

Detecting cell lysis using viscosity monitoring inE. colifermentation to prevent product loss

(2016) Joseph M. Newton et al.   BIOTECHNOLOGY PROGRESS

How to trigger periplasmic release in recombinantEscherichia coli: A comparative analysis

(2016) David J. Wurm et al.   ENGINEERING IN LIFE SCIENCES

Generic biomass estimation methods targeting physiologic process control in induced bacterial cultures

(2016) Wieland N. Reichelt et al.   ENGINEERING IN LIFE SCIENCES

Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli ‘leaky mutant’

(2016) Jakob Michael Müller et al.   JOURNAL OF BIOTECHNOLOGY

Bioprocess monitoring: minimizing sample matrix effects for total protein quantification with bicinchoninic acid assay

(2016) Wieland N. Reichelt et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Monitoring the live to dead transition of bacteria during thermal stress by a multi-method approach

(2016) B. Kramer et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Application of a High Cell Density Capacitance Sensor to Different Microorganisms

(2016) Bernadett Kiss et al.   PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING

An efficient protocol to enhance the extracellular production of recombinant protein from Escherichia coli by the synergistic effects of sucrose, glycine, and Triton X-100

(2016) Ru-Meng Bao et al.   PROTEIN EXPRESSION AND PURIFICATION

A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

(2016) Christoph Slouka et al.   SENSORS

Ultrasound-Enhanced Attenuated Total Reflection Mid-infrared Spectroscopy In-Line Probe: Acquisition of Cell Spectra in a Bioreactor

(2015) Cosima Koch et al.   ANALYTICAL CHEMISTRY

Enhancing the selective extracellular location of a recombinant E. coli domain antibody by management of fermentation conditions

(2015) Ioannis Voulgaris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

In Situ Near-Infrared (NIR) versus High-Throughput Mid-Infrared (MIR) Spectroscopy to Monitor Biopharmaceutical Production

(2015) Kevin C. Sales et al.   APPLIED SPECTROSCOPY

Sensor systems for bioprocess monitoring

(2015) Philipp Biechele et al.   ENGINEERING IN LIFE SCIENCES

Quantification of cell lysis during CHO bioprocesses: Impact on cell count, growth kinetics and productivity

(2015) Tobias Klein et al.   JOURNAL OF BIOTECHNOLOGY

At-line mid infrared spectroscopy for monitoring downstream processing unit operations

(2015) Florian Capito et al.   PROCESS BIOCHEMISTRY

Universal Capacitance Model for Real-Time Biomass in Cell Culture

(2015) Viktor Konakovsky et al.   SENSORS

Scale-down effect on the extracellular proteome of Escherichia coli: correlation with membrane permeability and modulation according to substrate heterogeneities

(2014) Alison Brognaux et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Design, development, and validation of an in-situ biosensor array for metabolite monitoring of cell cultures

(2014) Cristina Boero et al.   BIOSENSORS & BIOELECTRONICS

Hybrid modeling for quality by design and PAT-benefits and challenges of applications in biopharmaceutical industry

(2014) Moritz von Stosch et al.   Biotechnology Journal

Low-frequency dielectric dispersion of bacterial cell suspensions

(2014) Koji Asami   COLLOIDS AND SURFACES B-BIOINTERFACES

On-line near infrared bioreactor monitoring of cell density and concentrations of glucose and lactate during insect cell cultivation

(2014) Jiang Qiu et al.   JOURNAL OF BIOTECHNOLOGY

Screen-printed electrodes for biosensing: a review (2008–2013)

(2014) Zahra Taleat et al.   MICROCHIMICA ACTA

The Interrelationship between Promoter Strength, Gene Expression, and Growth Rate

(2014) Matthew S. Bienick et al.   PLoS One

Construction of leaky strains and extracellular production of exogenous proteins in recombinantEscherichia coli

(2014) Zhao-Yuan Chen et al.   Microbial Biotechnology

Multi-analyte quantification in bioprocesses by Fourier-transform-infrared spectroscopy by partial least squares regression and multivariate curve resolution

(2013) Cosima Koch et al.   ANALYTICA CHIMICA ACTA

Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions

(2013) Juergen Mairhofer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A novel strategy for enhancing extracellular secretion of recombinant proteins in Escherichia coli

(2013) Lingqia Su et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Tunable recombinant protein expression with E. coli in a mixed-feed environment

(2013) Patrick Sagmeister et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mid-infrared spectroscopy-based antibody aggregate quantification in cell culture fluids

(2013) Florian Capito et al.   Biotechnology Journal

Development of at-line assay to monitor charge variants of MAbs during production

(2013) M. M. St. Amand et al.   BIOTECHNOLOGY PROGRESS

Comparative study of non-invasive monitoring via infrared spectroscopy for mammalian cell cultivations

(2013) M. Sandor et al.   JOURNAL OF BIOTECHNOLOGY

Real-time monitoring of cell viability and cell density on the basis of a three dimensional optical reflectance method (3D-ORM): investigation of the effect of sub-lethal and lethal injuries

(2013) Alison Brognaux et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

(2013) Kaisa Ukkonen et al.   Microbial Cell Factories

A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors

(2013) Alison Brognaux et al.   Microbial Cell Factories

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Chemometrics and in-line near infrared spectroscopic monitoring of a biopharmaceutical Chinese hamster ovary cell culture: Prediction of multiple cultivation variables

(2013) Matthieu Clavaud et al.   TALANTA

Ultrasonic Manipulation of Yeast Cells in Suspension for Absorption Spectroscopy with an Immersible Mid-Infrared Fiberoptic Probe

(2013) Cosima Koch et al.   ULTRASOUND IN MEDICINE AND BIOLOGY

Pre-Calibrated Biosensors for Single-Use Applications

(2012) Isabella Moser et al.   CHEMIE INGENIEUR TECHNIK

Combining light microscopy, dielectric spectroscopy, MALDI intact cell mass spectrometry, FTIR spectromicroscopy and multivariate data mining for morphological and physiological bioprocess characterization of filamentous organisms

(2012) Andreas E. Posch et al.   FUNGAL GENETICS AND BIOLOGY

On-line multiple component analysis for efficient quantitative bioprocess development

(2012) Christian Dietzsch et al.   JOURNAL OF BIOTECHNOLOGY

A new set up for multi-analyte sensing: At-line bio-process monitoring

(2011) Mehriban Akin et al.   BIOSENSORS & BIOELECTRONICS

Green fluorescent protein (GFP) leakage from microbial biosensors provides useful information for the evaluation of the scale-down effect

(2011) Frank Delvigne et al.   Biotechnology Journal

Capacitive immunosensor for the detection of host cell proteins

(2011) Kosin Teeparuksapun et al.   JOURNAL OF BIOTECHNOLOGY

Linear Correlation between Online Capacitance and Offline Biomass Measurement up to High Cell Densities in Escherichia coli Fermentations in a Pilot-Scale Pressurized Bioreactor

(2011) Ingo Knabben   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant Fab expression and secretion in Escherichia coli continuous culture at medium cell densities: Influence of temperature

(2011) Escarlata Rodríguez-Carmona et al.   PROCESS BIOCHEMISTRY

Process analytical technology (PAT) for biopharmaceutical products

(2010) A. S. Rathore et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

On-line infrared spectroscopy for bioprocess monitoring

(2010) Daniel Landgrebe et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

(2010) Michal Dabros et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Chemical and biological characterisation of a sensor surface for bioprocess monitoring

(2010) Jonathan D. Moore et al.   BIOSENSORS & BIOELECTRONICS

Electrochemical biosensors

(2010) Niina J. Ronkainen et al.   CHEMICAL SOCIETY REVIEWS

Viability states of bacteria-Specific mechanisms of selected probes

(2010) Heike Sträuber et al.   CYTOMETRY PART A

The Bacterial Cell Envelope

(2010) T. J. Silhavy et al.   Cold Spring Harbor Perspectives in Biology

Application of flow cytometry to industrial microbial bioprocesses

(2009) Mario Díaz et al.   BIOCHEMICAL ENGINEERING JOURNAL

Quantifying the membrane potential during E. coli growth stages

(2009) Corina Teodora Bot et al.   BIOPHYSICAL CHEMISTRY

Probing the membrane potential of living cells by dielectric spectroscopy

(2009) Corina Bot et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Induction of membrane permeability inEscherichia coli mediated by lysis protein of the ColE7 operon

(2009) Lung J.R. Lin et al.   FEMS MICROBIOLOGY LETTERS

Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins

(2009) Benjamin Sommer et al.   JOURNAL OF BIOTECHNOLOGY

Correcting the polarization effect in very low frequency dielectric spectroscopy

(2009) Camelia Prodan et al.   JOURNAL OF PHYSICS D-APPLIED PHYSICS

Advances in product release strategies and impact on bioprocess design

(2009) Bangaru Balasundaram et al.   TRENDS IN BIOTECHNOLOGY

At-line quantification of bioactive antibody in bioreactor by surface plasmon resonance using epitope detection

(2008) Natalie Chavane et al.   ANALYTICAL BIOCHEMISTRY

Electrochemical Biosensors for Medical and Food Applications

(2008) Minhaz Uddin Ahmed et al.   ELECTROANALYSIS

Applications of PAT-Process Analytical Technology in Recombinant Protein Processes withEscherichia coli

(2008) C. Kaiser et al.   ENGINEERING IN LIFE SCIENCES

A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device

(2008) Jana Šefčovičová et al.   ENZYME AND MICROBIAL TECHNOLOGY

Fedbatch design for periplasmic product retention in Escherichia coli

(2008) Emma Bäcklund et al.   JOURNAL OF BIOTECHNOLOGY

At-line monitoring of bioreactor protein production by surface plasmon resonance

(2007) R. Jacquemart et al.   BIOTECHNOLOGY AND BIOENGINEERING