Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins

(2018) Federica Angius et al.   Scientific Reports

Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor

(2017) Seong Keun Kim et al.   ACS Synthetic Biology

Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints

(2016) Lukas Marschall et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes

(2015) Csilla Toeroek et al.   SpringerPlus

Preventing T7 RNA Polymerase Read-through Transcription—A Synthetic Termination Signal Capable of Improving Bioprocess Stability

(2014) Juergen Mairhofer et al.   ACS Synthetic Biology

Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

(2014) Daniel Camsund et al.   Journal of Biological Engineering

Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions

(2013) Juergen Mairhofer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The effect of LacI autoregulation on the performance of the lactose utilization system in Escherichia coli

(2013) S. Semsey et al.   NUCLEIC ACIDS RESEARCH

Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli

(2012) Robert C. Brewster et al.   PLoS Computational Biology

Quantitative dissection of the simple repression input-output function

(2011) H. G. Garcia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Promoter knock-in: a novel rational method for the fine tuning of genes

(2010) Marjan De Mey et al.   BMC BIOTECHNOLOGY

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

(2009) Frank Kensy et al.   Microbial Cell Factories

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols