Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

A fed-batch based cultivation mode inEscherichia coliresults in improved specific activity of a novel chimeric-truncated form of tissue plasminogen activator

(2012) F. Mahboudi et al.   JOURNAL OF APPLIED MICROBIOLOGY

Expression of high-affinity human antibody fragments in bacteria

(2012) Romain Rouet et al.   Nature Protocols

Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation

(2011) Julia Glazyrina et al.   Biotechnology Journal

Application of a Fab fragment of monoclonal antibody specific to N-terminal pro-brain natriuretic peptide for the detection based on regeneration-free electrochemical immunosensor

(2011) Weijing Yi et al.   BIOTECHNOLOGY LETTERS

Transcript Profiling and Inference ofEscherichia coliK-12 ArcA Activity across the Range of Physiologically Relevant Oxygen Concentrations

(2011) Matthew D. Rolfe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

(2011) Juozas Šiurkus et al.   Microbial Cell Factories

Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression

(2011) Juozas Šiurkus et al.   Microbial Cell Factories

High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

(2011) Kaisa Ukkonen et al.   Microbial Cell Factories

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system

(2011) Julia Glazyrina et al.   New Biotechnology

Ligand-Induced Formation of a Transient Tryptophan Synthase Complex with αββ Subunit Stoichiometry

(2010) Alexander Ehrmann et al.   BIOCHEMISTRY

Electrooptical monitoring of cell polarizability and cell size in aerobic Escherichia coli batch cultivations

(2010) Stefan Junne et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor

(2010) Julia Glazyrina et al.   Microbial Cell Factories

Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

(2010) Juozas Šiurkus et al.   Microbial Cell Factories

A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

(2010) Mirja Krause et al.   Microbial Cell Factories

Bacterial production and functional characterization of the Fab fragment of the murine IgG1/  monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics

(2010) L. Friedrich et al.   PROTEIN ENGINEERING DESIGN & SELECTION

High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli

(2008) Tingmei Ye et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Fedbatch design for periplasmic product retention in Escherichia coli

(2008) Emma Bäcklund et al.   JOURNAL OF BIOTECHNOLOGY

Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

(2008) Johanna Panula-Perälä et al.   Microbial Cell Factories