Tuning recombinant protein expression to match secretion capacity

Antibodies to watch in 2018

(2018) Hélène Kaplon et al.   mAbs

Signal peptides for recombinant protein secretion in bacterial expression systems

(2018) Roland Freudl   Microbial Cell Factories

Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins

(2018) Rosa Morra et al.   mBio

A Dynamic Model of Resource Allocation in Response to the Presence of a Synthetic Construct

(2018) Axel Nyström et al.   ACS Synthetic Biology

Rationalizing Context-Dependent Performance of Dynamic RNA Regulatory Devices

(2018) Ross Kent et al.   ACS Synthetic Biology

Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress

(2018) Thomas Baumgarten et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv

(2017) Anna Gąciarz et al.   PLoS One

Predicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli

(2017) Jian Tian et al.   Scientific Reports

Tunable recombinant protein expression in E. coli: enabler for continuous processing?

(2016) Lukas Marschall et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metabolomic analysis of riboswitch containing E. coli recombinant expression system

(2016) Howbeer Muhamadali et al.   Molecular BioSystems

Codon influence on protein expression in E. coli correlates with mRNA levels

(2016) Grégory Boël et al.   NATURE

Protein export through the bacterial Sec pathway

(2016) Alexandra Tsirigotaki et al.   NATURE REVIEWS MICROBIOLOGY

Challenges to production of antibodies in bacteria and yeast

(2015) Yong Jae Lee et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Secretion systems in Gram-negative bacteria: structural and mechanistic insights

(2015) Tiago R. D. Costa et al.   NATURE REVIEWS MICROBIOLOGY

Dual transcriptional-translational cascade permits cellular level tuneable expression control

(2015) Rosa Morra et al.   NUCLEIC ACIDS RESEARCH

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo

(2014) Sebastian Pechmann et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Multiple roles of the coding sequence 5′ end in gene expression regulation

(2014) Tamir Tuller et al.   NUCLEIC ACIDS RESEARCH

Co-expression of Dsb proteins enables soluble expression of a single-chain variable fragment (scFv) against human type 1 insulin-like growth factor receptor (IGF-1R) in E. coli

(2014) Xue-Wen Sun et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Optimisation of signal peptide for recombinant protein secretion in bacterial hosts

(2013) Kheng Oon Low et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhanced production of antibody fragment via SRP pathway engineering in Escherichia coli

(2013) Yong Jae Lee et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Enhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm

(2013) Raphael Levy et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Precise and reliable gene expression via standard transcription and translation initiation elements

(2013) Vivek K Mutalik et al.   NATURE METHODS

Molecular crowding limits translation and cell growth

(2013) S. Klumpp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction of an artificial secYEG operon allowing high level secretion of α-amylase

(2013) Kelly C.L. Mulder et al.   PROTEIN EXPRESSION AND PURIFICATION

Causes and Effects of N-Terminal Codon Bias in Bacterial Genes

(2013) D. B. Goodman et al.   SCIENCE

Contextualizing context for synthetic biology - identifying causes of failure of synthetic biological systems

(2012) Stefano Cardinale et al.   Biotechnology Journal

Directed evolution of efficient secretion in the SRP-dependent export of TolB

(2011) Yaramah M. Zalucki et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Reduced mRNA Secondary-Structure Stability Near the Start Codon Indicates Functional Genes in Prokaryotes

(2011) Thomas E. Keller et al.   Genome Biology and Evolution

Effects of cytoplasmic and periplasmic chaperones on secretory production of single-chain Fv antibody in Escherichia coli

(2011) Hiroyuki Sonoda et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Strain engineering for improved expression of recombinant proteins in bacteria

(2011) Tomohiro Makino et al.   Microbial Cell Factories

An Evolutionarily Conserved Mechanism for Controlling the Efficiency of Protein Translation

(2010) Tamir Tuller et al.   CELL

Antibody fragments

(2010) Aaron L. Nelson   mAbs

Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels

(2009) Nathan Scott et al.   BMC BIOTECHNOLOGY

Microbial factories for recombinant pharmaceuticals

(2009) Neus Ferrer-Miralles et al.   Microbial Cell Factories

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Biased codon usage in signal peptides: a role in protein export

(2009) Yaramah M. Zalucki et al.   TRENDS IN MICROBIOLOGY

Studies related to antibody fragment (Fab) production inEscherichia coliW3110 fed-batch fermentation processes using multiparameter flow cytometry

(2008) Andrew Want et al.   CYTOMETRY PART A

Fedbatch design for periplasmic product retention in Escherichia coli

(2008) Emma Bäcklund et al.   JOURNAL OF BIOTECHNOLOGY

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

(2008) Brigitte Gasser et al.   Microbial Cell Factories

SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli

(2008) Holger Thie et al.   New Biotechnology