Repurposing Modular Polyketide Synthases and Non-ribosomal Peptide Synthetases for Novel Chemical Biosynthesis

Adenylation domains in nonribosomal peptide engineering

(2019) Hajo Kries et al.   CHEMBIOCHEM

Engineering the Substrate Specificity of a Modular Polyketide Synthase for Installation of Consecutive Non-Natural Extender Units

(2019) Edward Kalkreuter et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Refining and expanding nonribosomal peptide synthetase function and mechanism

(2019) Matt McErlean et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Engineering of Chimeric Polyketide Synthases Using SYNZIP Docking Domains

(2019) Maja Klaus et al.   ACS Chemical Biology

Synthetic Biology Tools for Novel Secondary Metabolite Discovery in Streptomyces

(2019) Namil Lee et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline

(2019) Kai Blin et al.   NUCLEIC ACIDS RESEARCH

Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform

(2019) Bo Pang et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Modification and de novo design of non-ribosomal peptide synthetases using specific assembly points within condensation domains

(2019) Kenan A. J. Bozhüyük et al.   Nature Chemistry

Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

(2019) Fuchao Xu et al.   Journal of Biological Engineering

Fungal indole alkaloid biogenesis through evolution of a bifunctional reductase/Diels–Alderase

(2019) Qingyun Dan et al.   Nature Chemistry

A computational framework to explore large-scale biosynthetic diversity

(2019) Jorge C. Navarro-Muñoz et al.   Nature Chemical Biology

GenBank

(2019) Eric W Sayers et al.   NUCLEIC ACIDS RESEARCH

Repurposing the GNAT Fold in the Initiation of Polyketide Biosynthesis

(2019) Meredith A. Skiba et al.   STRUCTURE

Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases

(2018) Luyun Zhang et al.   ACS Chemical Biology

Polyketide synthases as a platform for chemical product design

(2018) Amin Zargar et al.   AICHE JOURNAL

Structural Basis of a Broadly Selective Acyltransferase from the Polyketide Synthase of Splenocin

(2018) Yuan Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering strategies for rational polyketide synthase design

(2018) Maja Klaus et al.   NATURAL PRODUCT REPORTS

SWISS-MODEL: homology modelling of protein structures and complexes

(2018) Andrew Waterhouse et al.   NUCLEIC ACIDS RESEARCH

Engineering Bifunctional Enzymes Capable of Adenylating and Selectively Methylating the Side Chain or Core of Amino Acids

(2018) Taylor A. Lundy et al.   ACS Synthetic Biology

Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

(2018) Alistair S. Brown et al.   NATURAL PRODUCT REPORTS

Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

(2018) Satoshi Yuzawa et al.   Nature Communications

Insights into Substrate Modification by Dehydratases from Type I Polyketide Synthases

(2017) Alexandre Faille et al.   JOURNAL OF MOLECULAR BIOLOGY

Towards Precision Engineering of Canonical Polyketide Synthase Domains: Recent Advances and Future Prospects

(2017) Carmen Bayly et al.   MOLECULES

FRET monitoring of a nonribosomal peptide synthetase

(2017) Jonas Alfermann et al.   Nature Chemical Biology

De novo design and engineering of non-ribosomal peptide synthetases

(2017) Kenan A. J. Bozhüyük et al.   Nature Chemistry

ClusterCAD: a computational platform for type I modular polyketide synthase design

(2017) Clara H Eng et al.   NUCLEIC ACIDS RESEARCH

X-Ray Crystallography and Electron Microscopy of Cross- and Multi-Module Nonribosomal Peptide Synthetase Proteins Reveal a Flexible Architecture

(2017) Michael J. Tarry et al.   STRUCTURE

An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli

(2017) Sara Cleto et al.   mBio

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

(2017) Randall A. Hughes et al.   Cold Spring Harbor Perspectives in Biology

Development of Transcription Factor-Based Designer Macrolide Biosensors for Metabolic Engineering and Synthetic Biology

(2017) Christian M. Kasey et al.   ACS Synthetic Biology

Inversion of Extender Unit Selectivity in the Erythromycin Polyketide Synthase by Acyltransferase Domain Engineering

(2016) Irina Koryakina et al.   ACS Chemical Biology

Alteration of Polyketide Stereochemistry from anti to syn by a Ketoreductase Domain Exchange in a Type I Modular Polyketide Synthase Subunit

(2016) Clara H. Eng et al.   BIOCHEMISTRY

Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation

(2016) Annabel C. Murphy et al.   CHEMICAL COMMUNICATIONS

Bio-based production of fuels and industrial chemicals by repurposing antibiotic-producing type I modular polyketide synthases: opportunities and challenges

(2016) Satoshi Yuzawa et al.   JOURNAL OF ANTIBIOTICS

Protein-Protein Interactions, Not Substrate Recognition, Dominate the Turnover of Chimeric Assembly Line Polyketide Synthases

(2016) Maja Klaus et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Thioesterase domain swapping of a linear polyketide tautomycetin with a macrocyclic polyketide pikromycin in Streptomyces sp. CK4412

(2016) Ashootosh Tripathi et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Recent advances in engineering nonribosomal peptide assembly lines

(2016) M. Winn et al.   NATURAL PRODUCT REPORTS

Insights into the chemical logic and enzymatic machinery of NRPS assembly lines

(2016) Christopher T. Walsh   NATURAL PRODUCT REPORTS

Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology

(2016) Kira J. Weissman   NATURAL PRODUCT REPORTS

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis

(2016) Akimasa Miyanaga et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production

(2016) Satoshi Yuzawa et al.   ACS Synthetic Biology

Structural and Functional Analysis of the Loading Acyltransferase from Avermectin Modular Polyketide Synthase

(2015) Fen Wang et al.   ACS Chemical Biology

Structural Elucidation of the Bispecificity of A Domains as a Basis for Activating Non-natural Amino Acids

(2015) Heidi Kaljunen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry

(2015) Thibault Annaval et al.   CHEMBIOCHEM

Comprehensive Structural and Biochemical Analysis of the Terminal Myxalamid Reductase Domain for the Engineered Production of Primary Alcohols

(2015) Jesus F. Barajas et al.   CHEMISTRY & BIOLOGY

Freedom and Constraint in Engineered Noncolinear Polyketide Assembly Lines

(2015) Yuki Sugimoto et al.   CHEMISTRY & BIOLOGY

Harnessing natural product assembly lines: structure, promiscuity, and engineering

(2015) Christopher C. Ladner et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Engineering an iterative polyketide pathway in Escherichia coli results in single-form alkene and alkane overproduction

(2015) Qian Liu et al.   METABOLIC ENGINEERING

Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids

(2015) James M. Clomburg et al.   METABOLIC ENGINEERING

Natural products as biofuels and bio-based chemicals: fatty acids and isoprenoids

(2015) Harry R. Beller et al.   NATURAL PRODUCT REPORTS

Computational approaches to natural product discovery

(2015) Marnix H Medema et al.   Nature Chemical Biology

Discovery of microbial natural products by activation of silent biosynthetic gene clusters

(2015) Peter J. Rutledge et al.   NATURE REVIEWS MICROBIOLOGY

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

(2015) Nicholas C.O. Lee et al.   NUCLEIC ACIDS RESEARCH

Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes

(2015) Tilmann Weber et al.   TRENDS IN BIOTECHNOLOGY

Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

(2015) Wenjun Jiang et al.   Nature Communications

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

(2015) Andrew Hagen et al.   ACS Synthetic Biology

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids

(2014) Hajo Kries et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biosynthesis of Novel Pyoverdines by Domain Substitution in a Nonribosomal Peptide Synthetase of Pseudomonas aeruginosa

(2014) Mark J. Calcott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

In Vitro Analysis of Carboxyacyl Substrate Tolerance in the Loading and First Extension Modules of Borrelidin Polyketide Synthase

(2014) Andrew Hagen et al.   BIOCHEMISTRY

Mapping a Ketosynthase:Acyl Carrier Protein Binding Interface via Unnatural Amino Acid-Mediated Photo-Cross-Linking

(2014) Zhixia Ye et al.   BIOCHEMISTRY

Reprogramming Acyl Carrier Protein Interactions of an Acyl-CoA Promiscuous trans-Acyltransferase

(2014) Zhixia Ye et al.   CHEMISTRY & BIOLOGY

Architectures of Whole-Module and Bimodular Proteins from the 6-Deoxyerythronolide B Synthase

(2014) Andrea L. Edwards et al.   JOURNAL OF MOLECULAR BIOLOGY

NRPS Substrate Promiscuity Leads to More Potent Antitubercular Sansanmycin Analogues

(2014) Yunying Xie et al.   JOURNAL OF NATURAL PRODUCTS

Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange

(2014) Ralf Beer et al.   Molecular BioSystems

Secondary metabolomics: the impact of mass spectrometry-based approaches on the discovery and characterization of microbial natural products

(2014) Daniel Krug et al.   NATURAL PRODUCT REPORTS

Structure of a modular polyketide synthase

(2014) Somnath Dutta et al.   NATURE

Structural rearrangements of a polyketide synthase module during its catalytic cycle

(2014) Jonathan R. Whicher et al.   NATURE

Bioretrosynthetic construction of a didanosine biosynthetic pathway

(2014) William R Birmingham et al.   Nature Chemical Biology

CDD: NCBI's conserved domain database

(2014) Aron Marchler-Bauer et al.   NUCLEIC ACIDS RESEARCH

Analysis of the linker region joining the adenylation and carrier protein domains of the modular nonribosomal peptide synthetases

(2014) Bradley R. Miller et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Biocatalysts for Natural Product Biosynthesis

(2014) Nidhi Tibrewal et al.   Annual Review of Chemical and Biomolecular Engineering

Structural Studies of an A2-Type Modular Polyketide Synthase Ketoreductase Reveal Features Controlling α-Substituent Stereochemistry

(2013) Jianting Zheng et al.   ACS Chemical Biology

Colorimetric Determination of Pyrophosphate Anion and Its Application to Adenylation Enzyme Assay

(2013) Hajime KATANO et al.   ANALYTICAL SCIENCES

Specific enrichment of nonribosomal peptide synthetase module by an affinity probe for adenylation domains

(2013) Fumihiro Ishikawa et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Engineering the Substrate Specificity of the DhbE Adenylation Domain by Yeast Cell Surface Display

(2013) Keya Zhang et al.   CHEMISTRY & BIOLOGY

In Vitro Reconstitution and Analysis of the 6-Deoxyerythronolide B Synthase

(2013) Brian Lowry et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli

(2013) T. P. Howard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Retropath: Automated Pipeline for Embedded Metabolic Circuits

(2013) Pablo Carbonell et al.   ACS Synthetic Biology

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

(2012) Jenny Thirlway et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Functional Modular Dissection of DEBS1-TE Changes Triketide Lactone Ratios and Provides Insight into Acyl Group Loading, Hydrolysis, and ACP Transfer

(2012) John Yan et al.   BIOCHEMISTRY

Role of a Conserved Arginine Residue in Linkers between the Ketosynthase and Acyltransferase Domains of Multimodular Polyketide Synthases

(2012) Satoshi Yuzawa et al.   BIOCHEMISTRY

Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains

(2012) Carter A. Mitchell et al.   BIOCHEMISTRY

Rigidifying Acyl Carrier Protein Domain in Iterative Type I PKS CalE8 Does Not Affect Its Function

(2012) Jackwee Lim et al.   BIOPHYSICAL JOURNAL

Site-directed modification of the adenylation domain of the fusaricidin nonribosomal peptide synthetase for enhanced production of fusaricidin analogs

(2012) Jae Woo Han et al.   BIOTECHNOLOGY LETTERS

Taxol Biosynthesis: Tyrocidine Synthetase A Catalyzes the Production of Phenylisoserinyl CoA and Other Amino Phenylpropanoyl Thioesters

(2012) Ruth Muchiri et al.   CHEMISTRY & BIOLOGY

Polyketide Proofreading by an Acyltransferase-like Enzyme

(2012) Katja Jensen et al.   CHEMISTRY & BIOLOGY

Structural and Functional Investigation of the Intermolecular Interaction between NRPS Adenylation and Carrier Protein Domains

(2012) Jesse A. Sundlov et al.   CHEMISTRY & BIOLOGY

Type III polyketide synthases in natural product biosynthesis

(2012) Dayu Yu et al.   IUBMB LIFE

Explorations of catalytic domains in non-ribosomal peptide synthetase enzymology

(2012) Gene H. Hur et al.   NATURAL PRODUCT REPORTS

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

6-Deoxyerythronolide B Synthase Thioesterase-Catalyzed Macrocyclization Is Highly Stereoselective

(2012) Atahualpa Pinto et al.   ORGANIC LETTERS

Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains

(2012) Max Crüsemann et al.   Chemical Science

Directed Evolution of a Gatekeeper Domain in Nonribosomal Peptide Synthesis

(2011) Benoit Villiers et al.   CHEMISTRY & BIOLOGY

Characterization of the N-Methyltransferase Activities of the Multifunctional Polypeptide Cyclosporin Synthetase

(2011) Tony Velkov et al.   CHEMISTRY & BIOLOGY

Directed Evolution of the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives In Vivo

(2011) Bradley S. Evans et al.   CHEMISTRY & BIOLOGY

Industrial biotechnology—the future of green chemistry?

(2011) Stefanie Wenda et al.   GREEN CHEMISTRY

NRPSpredictor2—a web server for predicting NRPS adenylation domain specificity

(2011) Marc Röttig et al.   NUCLEIC ACIDS RESEARCH

A Fungal Nonribosomal Peptide Synthetase Module that can Synthesize Thiopyrazines

(2011) Kangjian Qiao et al.   ORGANIC LETTERS

NRPS Substrate Promiscuity Diversifies the Xenematides

(2011) Jason M. Crawford et al.   ORGANIC LETTERS

A continuous kinetic assay for adenylation enzyme activity and inhibition

(2010) Daniel J. Wilson et al.   ANALYTICAL BIOCHEMISTRY

Genetically Engineered Lipopeptide Antibiotics Related to A54145 and Daptomycin with Improved Properties

(2010) K. T. Nguyen et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Widespread Head-to-Head Hydrocarbon Biosynthesis in Bacteria and Role of OleA

(2010) D. J. Sukovich et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biosynthesis of the Allylmalonyl-CoA Extender Unit for the FK506 Polyketide Synthase Proceeds through a Dedicated Polyketide Synthase and Facilitates the Mutasynthesis of Analogues

(2010) SangJoon Mo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

I-TASSER: a unified platform for automated protein structure and function prediction

(2010) Ambrish Roy et al.   Nature Protocols

Structural Basis for Binding Specificity between Subclasses of Modular Polyketide Synthase Docking Domains

(2009) Tonia J. Buchholz et al.   ACS Chemical Biology

Genes Involved in Long-Chain Alkene Biosynthesis in Micrococcus luteus

(2009) H. R. Beller et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cyclopiazonic Acid Biosynthesis inAspergillussp.: Characterization of a Reductase-like R* Domain in Cyclopiazonate Synthetase That Forms and Releasescyclo-Acetoacetyl-l-tryptophan

(2009) Xinyu Liu et al.   BIOCHEMISTRY

New, Highly Active Nonbenzoquinone Geldanamycin Derivatives by Using Mutasynthesis

(2009) Simone Eichner et al.   CHEMBIOCHEM

Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA

(2009) Benoit R. M. Villiers et al.   CHEMBIOCHEM

Opportunities for enzyme engineering in natural product biosynthesis

(2009) Peter Bernhardt et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Precursor-directed biosynthesis of fluorinated iturin A in Bacillus spp.

(2009) Stephen Moran et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Mutasynthesis of Fluorosalinosporamide, a Potent and Reversible Inhibitor of the Proteasome

(2008) Alessandra S. Eustáquio et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Module Extension of a Non-Ribosomal Peptide Synthetase of the Glycopeptide Antibiotic Balhimycin Produced byAmycolatopsis balhimycina

(2008) Diane Butz et al.   CHEMBIOCHEM

Incomplete β-Ketone Processing as a Mechanism for Polyene Structural Variation in the FR-008/Candicidin Complex

(2008) Yongjun Zhou et al.   CHEMISTRY & BIOLOGY

Prediction and Manipulation of the Stereochemistry of Enoylreduction in Modular Polyketide Synthases

(2008) David H. Kwan et al.   CHEMISTRY & BIOLOGY

Biosynthesis of polyketide synthase extender units

(2008) Yolande A. Chan et al.   NATURAL PRODUCT REPORTS

A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis

(2008) J. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism

(2008) S. G. Van Lanen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of the Termination Module of a Nonribosomal Peptide Synthetase

(2008) A. Tanovic et al.   SCIENCE

Top-down mass spectrometry on low-resolution instruments: Characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways

(2007) Dario Meluzzi et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

(2007) Hrvoje Petković et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Precursor-Directed Biosynthesis of Novel Triketide Lactones

(2004) Rika Regentin et al.   BIOTECHNOLOGY PROGRESS