Bio-based production of fuels and industrial chemicals by repurposing antibiotic-producing type I modular polyketide synthases: opportunities and challenges

Alteration of Polyketide Stereochemistry from anti to syn by a Ketoreductase Domain Exchange in a Type I Modular Polyketide Synthase Subunit

(2016) Clara H. Eng et al.   BIOCHEMISTRY

Insights into polyketide biosynthesis gained from repurposing antibiotic-producing polyketide synthases to produce fuels and chemicals

(2016) Satoshi Yuzawa et al.   JOURNAL OF ANTIBIOTICS

Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts

(2016) Weishan Wang et al.   ACS Synthetic Biology

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Divergent Mechanistic Routes for the Formation ofgem-Dimethyl Groups in the Biosynthesis of Complex Polyketides

(2015) Sean Poust et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry

(2015) Thibault Annaval et al.   CHEMBIOCHEM

Alkane production from biomass: chemo-, bio- and integrated catalytic approaches

(2015) Aron Deneyer et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Uncovering the Formation and Selection of Benzylmalonyl-CoA from the Biosynthesis of Splenocin and Enterocin Reveals a Versatile Way to Introduce Amino Acids into Polyketide Carbon Scaffolds

(2015) Chenchen Chang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Natural products as biofuels and bio-based chemicals: fatty acids and isoprenoids

(2015) Harry R. Beller et al.   NATURAL PRODUCT REPORTS

Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products inStreptomyces

(2015) Chaoxian Bai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus

(2015) Yunzi Luo et al.   ACS Synthetic Biology

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid

(2015) Andrew Hagen et al.   ACS Synthetic Biology

In Vitro Analysis of Carboxyacyl Substrate Tolerance in the Loading and First Extension Modules of Borrelidin Polyketide Synthase

(2014) Andrew Hagen et al.   BIOCHEMISTRY

Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies

(2014) Ee-Been Goh et al.   METABOLIC ENGINEERING

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Broad Substrate Specificity of the Loading Didomain of the Lipomycin Polyketide Synthase

(2013) Satoshi Yuzawa et al.   BIOCHEMISTRY

Enzyme analysis of the polyketide synthase leads to the discovery of a novel analog of the antibiotic α-lipomycin

(2013) Satoshi Yuzawa et al.   JOURNAL OF ANTIBIOTICS

Engineering the acyltransferase substrate specificity of assembly line polyketide synthases

(2013) B. J. Dunn et al.   Journal of the Royal Society Interface

Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes

(2013) Theresa Siegl et al.   METABOLIC ENGINEERING

Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli

(2012) Satoshi Yuzawa et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels

(2011) Ee-Been Goh et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The construction of a library of synthetic promoters revealed some specific features of strong Streptomyces promoters

(2011) Nicolas Seghezzi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Beyond ethylmalonyl-CoA: The functional role of crotonyl-CoAcarboxylase/reductase homologs in expanding polyketide diversity

(2011) Micheal C. Wilson et al.   NATURAL PRODUCT REPORTS

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters

(2010) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Characterization of Tiacumicin B Biosynthetic Gene Cluster Affording Diversified Tiacumicin Analogues and Revealing a Tailoring Dihalogenase

(2010) Yi Xiao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Polylinker Approach to Reductive Loop Swaps in Modular Polyketide Synthases

(2008) Laurenz Kellenberger et al.   CHEMBIOCHEM