Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

Antisense transcription as a tool to tune gene expression

(2016) J. A. Brophy et al.   Molecular Systems Biology

Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins

(2016) Nicholas C. Tang et al.   NATURE MATERIALS

Design and synthesis of a minimal bacterial genome

(2016) C. A. Hutchison et al.   SCIENCE

Genetic circuit design automation

(2016) A. A. K. Nielsen et al.   SCIENCE

Synthetic mixed-signal computation in living cells

(2016) Jacob R. Rubens et al.   Nature Communications

Advances in synthetic dynamic circuits design: using novel synthetic parts to engineer new generations of gene oscillations

(2015) Steven William Sowa et al.   CURRENT OPINION IN BIOTECHNOLOGY

Multiplex pairwise assembly of array-derived DNA oligonucleotides

(2015) Jason C. Klein et al.   NUCLEIC ACIDS RESEARCH

Large-scale de novo DNA synthesis: technologies and applications

(2014) Sriram Kosuri et al.   NATURE METHODS

Unique nucleotide sequence–guided assembly of repetitive DNA parts for synthetic biology applications

(2014) Joseph P Torella et al.   Nature Protocols

Error removal in microchip-synthesized DNA using immobilized MutS

(2014) Wen Wan et al.   NUCLEIC ACIDS RESEARCH

PaperClip: rapid multi-part DNA assembly from existing libraries

(2014) Maryia Trubitsyna et al.   NUCLEIC ACIDS RESEARCH

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Rapid and Reliable DNA Assembly via Ligase Cycling Reaction

(2014) Stefan de Kok et al.   ACS Synthetic Biology

Synthesis of DNA/RNA and Their Analogs via Phosphoramidite and H-Phosphonate Chemistries

(2013) Subhadeep Roy et al.   MOLECULES

Rapid, modular and reliable construction of complex mammalian gene circuits

(2013) Patrick Guye et al.   NUCLEIC ACIDS RESEARCH

Synthetic Generation of Influenza Vaccine Viruses for Rapid Response to Pandemics

(2013) P. R. Dormitzer et al.   Science Translational Medicine

DNA synthesis, assembly and applications in synthetic biology

(2012) Siying Ma et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Massively parallel functional dissection of mammalian enhancers in vivo

(2012) Rupali P Patwardhan et al.   NATURE BIOTECHNOLOGY

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

(2012) Jerrod J Schwartz et al.   NATURE METHODS

‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules

(2012) Hwangbeom Kim et al.   NUCLEIC ACIDS RESEARCH

Genetic switchboard for synthetic biology applications

(2012) J. M. Callura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

(2012) K. Temme et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The rise of a digital immune system

(2012) Michael C Schatz et al.   GigaScience

A brief review of DNA and RNA chemical synthesis: Figure 1

(2011) Marvin H. Caruthers   BIOCHEMICAL SOCIETY TRANSACTIONS

Parallel on-chip gene synthesis and application to optimization of protein expression

(2011) Jiayuan Quan et al.   NATURE BIOTECHNOLOGY

Error correction of microchip synthesized genes using Surveyor nuclease

(2011) Ishtiaq Saaem et al.   NUCLEIC ACIDS RESEARCH

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

In situ Synthesis of DNA Microarray on Functionalized Cyclic Olefin Copolymer Substrate

(2010) Ishtiaq Saaem et al.   ACS Applied Materials & Interfaces

High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

(2010) Mark Matzas et al.   NATURE BIOTECHNOLOGY

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

(2010) Sriram Kosuri et al.   NATURE BIOTECHNOLOGY

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

Chemical synthesis of the mouse mitochondrial genome

(2010) Daniel G Gibson et al.   NATURE METHODS

Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

(2010) Emily M. LeProust et al.   NUCLEIC ACIDS RESEARCH

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

(2009) Daniel G. Gibson   NUCLEIC ACIDS RESEARCH

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

(2008) D. G. Gibson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE

Gene synthesis demystified

(2008) Michael J. Czar et al.   TRENDS IN BIOTECHNOLOGY

Engineering BioBrick vectors from BioBrick parts

(2008) Reshma P Shetty et al.   Journal of Biological Engineering

Non-polymerase-cycling-assembly-based chemical gene synthesis: Strategies, methods, and progress

(2007) Ai-Sheng Xiong et al.   BIOTECHNOLOGY ADVANCES