Oxidative stress‐alleviating strategies to improve recombinant protein production in CHO cells

Vitamins in cell culture media: Stability and stabilization strategies

(2019) Alisa Schnellbaecher et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic engineering of Chinese hamster ovary cells towards reduced biosynthesis and accumulation of novel growth inhibitors in fed-batch cultures

(2019) Bhanu Chandra Mulukutla et al.   METABOLIC ENGINEERING

Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

(2018) Sara Pereira et al.   Biotechnology Journal

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

(2018) Luhong He et al.   Biotechnology Journal

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

(2018) David Reinhart et al.   Biotechnology Journal

Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy

(2018) Sven Mathias et al.   JOURNAL OF BIOTECHNOLOGY

Improving titer while maintaining quality of final formulated drug substance via optimization of CHO cell culture conditions in low-iron chemically defined media

(2018) Jianlin Xu et al.   mAbs

Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer

(2018) Asokan Devarajan et al.   Cell Death & Disease

Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

(2018) Sara Pereira et al.   Biotechnology Journal

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

(2018) Luhong He et al.   Biotechnology Journal

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

(2018) David Reinhart et al.   Biotechnology Journal

Reduced cytotoxicity in PCB-exposed Chinese Hamster Ovary (CHO) cells pretreated with vitamin E

(2017) Teuta Murati et al.   FOOD AND CHEMICAL TOXICOLOGY

Impact of S-sulfocysteine on fragments and trisulfide bond linkages in monoclonal antibodies

(2017) Ronja Seibel et al.   mAbs

Oxidative stress-induced diseases and tea polyphenols

(2017) Xiangbing Mao et al.   Oncotarget

Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity

(2017) Tae Kwang Ha et al.   Biotechnology Journal

Microheterogeneity of Recombinant Antibodies: Analytics and Functional Impact

(2017) Beate Beyer et al.   Biotechnology Journal

Cell Factory Engineering

(2017) Anne Mathilde Davy et al.   Cell Systems

Advances in recombinant antibody manufacturing

(2016) Renate Kunert et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protein oxidation and peroxidation

(2016) M. J. Davies   BIOCHEMICAL JOURNAL

Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state

(2016) Sunita Maharjan et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis

(2016) Marc Feary et al.   BIOTECHNOLOGY PROGRESS

Stability of folic acid under several parameters

(2016) Amirah Mohd Gazzali et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Investigating mitochondrial redox state using NADH and NADPH autofluorescence

(2016) Thomas S. Blacker et al.   FREE RADICAL BIOLOGY AND MEDICINE

Endoplasmic Reticulum Stress and Associated ROS

(2016) Hafiz Zeeshan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

S-Sulfocysteine simplifies fed-batch processes and increases the CHO specific productivity via anti-oxidant activity

(2016) Caroline Hecklau et al.   JOURNAL OF BIOTECHNOLOGY

Folic acid and its photoproducts, 6-formylpterin and pterin-6-carboxylic acid, as generators of reactive oxygen species in skin cells during UVA exposure

(2016) Asta Juzeniene et al.   JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY

Understanding and Controlling Sialylation in a CHO Fc-Fusion Process

(2016) Amanda M. Lewis et al.   PLoS One

Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype

(2015) Paul S. Kelly et al.   Biotechnology Journal

Cell culture media supplementation of bioflavonoids for the targeted reduction of acidic species charge variants on recombinant therapeutic proteins

(2015) Patrick Hossler et al.   BIOTECHNOLOGY PROGRESS

Factors that determine stability of highly concentrated chemically defined production media

(2015) Rebecca E. McCoy et al.   BIOTECHNOLOGY PROGRESS

Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium

(2015) Iwona Zwolak   BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

(2015) Jennifer Dumont et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Assessment of antioxidant properties of alpha-keto acids in vitro and in vivo

(2015) Maria M. Bayliak et al.   EUROPEAN FOOD RESEARCH AND TECHNOLOGY

High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis

(2015) Camila A. Orellana et al.   JOURNAL OF PROTEOME RESEARCH

Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype

(2015) Paul S. Kelly et al.   Biotechnology Journal

Antioxidant responses and cellular adjustments to oxidative stress

(2015) Cristina Espinosa-Diez et al.   Redox Biology

Altered cytotoxicity of ROS-inducing compounds by sodium pyruvate in cell culture medium depends on the location of ROS generation

(2015) Jessica L Kelts et al.   SpringerPlus

Endoplasmic Reticulum Stress and Oxidative Stress in Cell Fate Decision and Human Disease

(2014) Stewart Siyan Cao et al.   ANTIOXIDANTS & REDOX SIGNALING

Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene withBcl-xL

(2014) Matthew P. Zustiak et al.   Biotechnology Journal

Effect of ambient light on monoclonal antibody product quality during small-scale mammalian cell culture process in clear glass bioreactors

(2014) Mary Mallaney et al.   BIOTECHNOLOGY PROGRESS

Dynamics of unfolded protein response in recombinant CHO cells

(2014) Kamal Prashad et al.   CYTOTECHNOLOGY

Nox family NADPH oxidases: Molecular mechanisms of activation

(2014) Ralf P. Brandes et al.   FREE RADICAL BIOLOGY AND MEDICINE

Tracing Compartmentalized NADPH Metabolism in the Cytosol and Mitochondria of Mammalian Cells

(2014) Caroline A. Lewis et al.   MOLECULAR CELL

Analyzing Clonal Variation of Monoclonal Antibody-Producing CHO Cell Lines Using an In Silico Metabolomic Platform

(2014) Atefeh Ghorbaniaghdam et al.   PLoS One

Site-2 protease responds to oxidative stress and regulates oxidative injury in mammalian cells

(2014) Yong Gu et al.   Scientific Reports

Hydrogen peroxide sensing, signaling and regulation of transcription factors

(2014) H. Susana Marinho et al.   Redox Biology

Arginine Modifications by Methylglyoxal: Discovery in a Recombinant Monoclonal Antibody and Contribution to Acidic Species

(2013) Chris Chumsae et al.   ANALYTICAL CHEMISTRY

Thioredoxins, Glutaredoxins, and Peroxiredoxins—Molecular Mechanisms and Health Significance: from Cofactors to Antioxidants to Redox Signaling

(2013) Eva-Maria Hanschmann et al.   ANTIOXIDANTS & REDOX SIGNALING

Selenium Modulates Oxidative Stress-Induced TRPM2 Cation Channel Currents in Transfected Chinese Hamster Ovary Cells

(2013) Mustafa Nazıroğlu et al.   BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY

CHO microRNA engineering is growing up: Recent successes and future challenges

(2013) Vaibhav Jadhav et al.   BIOTECHNOLOGY ADVANCES

Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture

(2013) Neil Templeton et al.   BIOTECHNOLOGY AND BIOENGINEERING

Thiol/disulfide redox states in signaling and sensing

(2013) Young-Mi Go et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Improved antibody production in Chinese hamster ovary cells by ATF4 overexpression

(2013) Ahmad M. Haredy et al.   CYTOTECHNOLOGY

The thioredoxin antioxidant system

(2013) Jun Lu et al.   FREE RADICAL BIOLOGY AND MEDICINE

Comparative analysis of amino acid metabolism and transport in CHO variants with different levels of productivity

(2013) Sarantos Kyriakopoulos et al.   JOURNAL OF BIOTECHNOLOGY

Fenton-like copper redox chemistry revisited: Hydrogen peroxide and superoxide mediation of copper-catalyzed oxidant production

(2013) A. Ninh Pham et al.   JOURNAL OF CATALYSIS

Brown drug substance color investigation in cell culture manufacturing using chemically defined media: A case study

(2013) Jianlin Xu et al.   PROCESS BIOCHEMISTRY

The Influence of Micronutrients in Cell Culture: A Reflection on Viability and Genomic Stability

(2013) Ana Lúcia Vargas Arigony et al.   Biomed Research International

Antioxidative properties of paraoxonase 2 in intestinal epithelial cells

(2012) Louis-Philippe Précourt et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Glutathione peroxidases

(2012) Regina Brigelius-Flohé et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Influence of Trace Elements on Stabilization of Aqueous Solutions of Ascorbic Acid

(2012) Barbara Dolińska et al.   BIOLOGICAL TRACE ELEMENT RESEARCH

LC-MS-based metabolic characterization of high monoclonal antibody-producing Chinese hamster ovary cells

(2012) William Pooi Kat Chong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Controlling trisulfide modification in recombinant monoclonal antibody produced in fed-batch cell culture

(2012) Rashmi Kshirsagar et al.   BIOTECHNOLOGY AND BIOENGINEERING

Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress

(2012) Keith EJ Tyo et al.   BMC BIOLOGY

The unfolded protein response: controlling cell fate decisions under ER stress and beyond

(2012) Claudio Hetz   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enhancing E. coli Tolerance towards Oxidative Stress via Engineering Its Global Regulator cAMP Receptor Protein (CRP)

(2012) Souvik Basak et al.   PLoS One

Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production

(2011) Chian Ju Jong et al.   AMINO ACIDS

Superoxide Dismutases: Role in Redox Signaling, Vascular Function, and Diseases

(2011) Tohru Fukai et al.   ANTIOXIDANTS & REDOX SIGNALING

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Regulation of cellular iron metabolism

(2011) Jian Wang et al.   BIOCHEMICAL JOURNAL

Paraoxonase 2 (PON2) in the mouse central nervous system: A neuroprotective role?

(2011) Gennaro Giordano et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Overexpression of taurine transporter in Chinese hamster ovary cells can enhance cell viability and product yield, while promoting glutamine consumption

(2010) Hisahiro Tabuchi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Role of iron and sodium citrate in animal protein-free CHO cell culture medium on cell growth and monoclonal antibody production

(2010) Yunling Bai et al.   BIOTECHNOLOGY PROGRESS

N-acetylcysteine, reactive oxygen species and beyond

(2010) Shi-Yong Sun   CANCER BIOLOGY & THERAPY

Crosstalk of reactive oxygen species and NF-κB signaling

(2010) Michael J Morgan et al.   CELL RESEARCH

Metabolic Engineering of Saccharomyces cerevisiae for Astaxanthin Production and Oxidative Stress Tolerance

(2009) K. Ukibe et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Artefacts in cell culture: Pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media

(2009) Lee Hua Long et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Generation of superoxide radicals by copper–glutathione complexes: Redox-consequences associated with their interaction with reduced glutathione

(2009) Hernán Speisky et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Scale-up analysis for a CHO cell culture process in large-scale bioreactors

(2009) Zizhuo Xing et al.   BIOTECHNOLOGY AND BIOENGINEERING

Experimental Evidence that Phenylalanine Provokes Oxidative Stress in Hippocampus and Cerebral Cortex of Developing Rats

(2009) Carolina G. Fernandes et al.   CELLULAR AND MOLECULAR NEUROBIOLOGY

Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

(2008) Chaya Mohan et al.   CELL STRESS & CHAPERONES

Overexpression of GADD34 Enhances Production of Recombinant Human Antithrombin III in Chinese Hamster Ovary Cells

(2008) Takeshi Omasa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Effects of N-acetylcysteine amide (NACA), a thiol antioxidant on radiation-induced cytotoxicity in Chinese hamster ovary cells

(2008) Wei Wu et al.   LIFE SCIENCES

Antioxidants reduce endoplasmic reticulum stress and improve protein secretion

(2008) J. D. Malhotra et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression

(2007) Tomoshi Ohya et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effect of Increased Expression of Protein Disulfide Isomerase and Heavy Chain Binding Protein on Antibody Secretion in a Recombinant CHO Cell Line

(2005) Nicole Borth et al.   BIOTECHNOLOGY PROGRESS

Effect of N-Acetylcystein on Butyrate-Treated Chinese Hamster Ovary Cells To Improve the Production of Recombinant Human Interferon-β-1a

(2005) Han Kyu Oh et al.   BIOTECHNOLOGY PROGRESS