Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

Application of 13 C flux analysis to identify high-productivity CHO metabolic phenotypes

(2017) Neil Templeton et al.   METABOLIC ENGINEERING

Amino acids in the cultivation of mammalian cells

(2016) Andrew Salazar et al.   AMINO ACIDS

Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement

(2016) Evan Wells et al.   Biotechnology Journal

KEGG: new perspectives on genomes, pathways, diseases and drugs

(2016) Minoru Kanehisa et al.   NUCLEIC ACIDS RESEARCH

Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement

(2016) Evan Wells et al.   Biotechnology Journal

A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism

(2016) Hooman Hefzi et al.   Cell Systems

Benchmarking of commercially available CHO cell culture media for antibody production

(2015) David Reinhart et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

The use of ‘Omics technology to rationally improve industrial mammalian cell line performance

(2015) Amanda M. Lewis et al.   BIOTECHNOLOGY AND BIOENGINEERING

CHOgenome.org 2.0: Genome resources and website updates

(2015) Benjamin G. Kremkow et al.   Biotechnology Journal

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives

(2015) Jae Seong Lee et al.   Biotechnology Journal

Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness

(2015) Christopher A. Sellick et al.   Biotechnology Journal

High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis

(2015) Camila A. Orellana et al.   JOURNAL OF PROTEOME RESEARCH

KEGG as a reference resource for gene and protein annotation

(2015) Minoru Kanehisa et al.   NUCLEIC ACIDS RESEARCH

The Reactome pathway Knowledgebase

(2015) Antonio Fabregat et al.   NUCLEIC ACIDS RESEARCH

CHOgenome.org 2.0: Genome resources and website updates

(2015) Benjamin G. Kremkow et al.   Biotechnology Journal

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives

(2015) Jae Seong Lee et al.   Biotechnology Journal

Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness

(2015) Christopher A. Sellick et al.   Biotechnology Journal

Multiplicity of Steady States in Glycolysis and Shift of Metabolic State in Cultured Mammalian Cells

(2015) Bhanu Chandra Mulukutla et al.   PLoS One

Amino acid and glucose metabolism in fed-batch CHO cell culture affects antibody production and glycosylation

(2014) Yuzhou Fan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic responses of CHO cells to limitation of key amino acids

(2014) Tiago M. Duarte et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effects of copper on CHO cells: Cellular requirements and product quality considerations

(2014) Inn H. Yuk et al.   BIOTECHNOLOGY PROGRESS

Effects of copper on CHO cells: Insights from gene expression analyses

(2014) Inn H. Yuk et al.   BIOTECHNOLOGY PROGRESS

Enhancement of production of protein biopharmaceuticals by mammalian cell cultures: the metabolomics perspective

(2014) Alan J Dickson   CURRENT OPINION IN BIOTECHNOLOGY

Complete Knockout of the Lactate Dehydrogenase A Gene is Lethal in Pyruvate Dehydrogenase Kinase 1, 2, 3 Down-Regulated CHO Cells

(2014) Shirley S. M. Yip et al.   MOLECULAR BIOTECHNOLOGY

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

(2013) Nuno Carinhas et al.   BIOTECHNOLOGY AND BIOENGINEERING

An 'omics approach towards CHO cell engineering

(2013) Payel Datta et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic analysis of antibody producing CHO cells in fed-batch production

(2013) Jason Dean et al.   BIOTECHNOLOGY AND BIOENGINEERING

Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture

(2013) Neil Templeton et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic flux rewiring in mammalian cell cultures

(2013) Jamey D Young   CURRENT OPINION IN BIOTECHNOLOGY

Advances in improving mammalian cells metabolism for recombinant protein production

(2013) Claudia Altamirano et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

Glutathione synthesis

(2012) Shelly C. Lu   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Glutathione levels in human tumors

(2012) Michael P. Gamcsik et al.   BIOMARKERS

Metabolite profiling of CHO cells with different growth characteristics

(2012) Stefanie Dietmair et al.   BIOTECHNOLOGY AND BIOENGINEERING

LC-MS-based metabolic characterization of high monoclonal antibody-producing Chinese hamster ovary cells

(2012) William Pooi Kat Chong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture

(2012) Suresh Selvarasu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Flux balance analysis of CHO cells before and after a metabolic switch from lactate production to consumption

(2012) Verónica S. Martínez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolomics profiling of extracellular metabolites in CHO-K1 cells cultured in different types of growth media

(2012) Salfarina Ezrina Mohmad-Saberi et al.   CYTOTECHNOLOGY

Multivariate analysis of cell culture bioprocess data—Lactate consumption as process indicator

(2012) Huong Le et al.   JOURNAL OF BIOTECHNOLOGY

On metabolic shift to lactate consumption in fed-batch culture of mammalian cells

(2012) Bhanu Chandra Mulukutla et al.   METABOLIC ENGINEERING

Parallel labeling experiments with [1,2-13C]glucose and [U-13C]glutamine provide new insights into CHO cell metabolism

(2012) Woo Suk Ahn et al.   METABOLIC ENGINEERING

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

(2012) Francesca Zagari et al.   New Biotechnology

HMDB 3.0—The Human Metabolome Database in 2013

(2012) David S. Wishart et al.   NUCLEIC ACIDS RESEARCH

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metabolite profiling of recombinant CHO cells: Designing tailored feeding regimes that enhance recombinant antibody production

(2011) Christopher A. Sellick et al.   BIOTECHNOLOGY AND BIOENGINEERING

Feeding lactate for CHO cell culture processes: Impact on culture metabolism and performance

(2011) Jincai Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Comparative metabolite analysis to understand lactate metabolism shift in Chinese hamster ovary cell culture process

(2011) Jun Luo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic Flux and the Regulation of Mammalian Cell Growth

(2011) Jason W. Locasale et al.   Cell Metabolism

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

(2011) Meixia Zhou et al.   JOURNAL OF BIOTECHNOLOGY

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Optimizing amino acid composition of CHO cell culture media for a fusion protein production

(2011) Zizhuo Xing et al.   PROCESS BIOCHEMISTRY

Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol

(2010) Stefanie Dietmair et al.   ANALYTICAL BIOCHEMISTRY

Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing

(2010) Andreas Berting et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: Overexpression of malate dehydrogenase II

(2010) William P.K. Chong et al.   JOURNAL OF BIOTECHNOLOGY

Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media

(2010) William P.K. Chong et al.   JOURNAL OF BIOTECHNOLOGY

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways

(2010) Bhanu Chandra Mulukutla et al.   TRENDS IN BIOTECHNOLOGY

A single nutrient feed supports both chemically defined NS0 and CHO fed-batch processes: Improved productivity and lactate metabolism

(2009) Ningning Ma et al.   BIOTECHNOLOGY PROGRESS

Applications of genome-scale metabolic reconstructions

(2009) Matthew A Oberhardt et al.   Molecular Systems Biology

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action

(2009) Royston Jefferis   TRENDS IN PHARMACOLOGICAL SCIENCES

Structure, function, and post-translational regulation of the catalytic and modifier subunits of glutamate cysteine ligase

(2008) Christopher C. Franklin et al.   MOLECULAR ASPECTS OF MEDICINE

HMDB: a knowledgebase for the human metabolome

(2008) D. S. Wishart et al.   NUCLEIC ACIDS RESEARCH