The limits to biocatalysis: pushing the envelope

Hot spots for the protein engineering of Baeyer-Villiger monooxygenases

(2018) Kathleen Balke et al.   BIOTECHNOLOGY ADVANCES

The emerging impact of cell-free chemical biosynthesis

(2018) Kristen M Wilding et al.   CURRENT OPINION IN BIOTECHNOLOGY

Evolving biocatalysis to meet bioeconomy challenges and opportunities

(2018) Alessandro Pellis et al.   New Biotechnology

Amine dehydrogenases: efficient biocatalysts for the reductive amination of carbonyl compounds

(2017) Tanja Knaus et al.   GREEN CHEMISTRY

Chemoselective, Enzymatic C–H Bond Amination Catalyzed by a Cytochrome P450 Containing an Ir(Me)-PIX Cofactor

(2017) Paweł Dydio et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highly Diastereo- and Enantioselective Synthesis of Trifluoromethyl-Substituted Cyclopropanes via Myoglobin-Catalyzed Transfer of Trifluoromethylcarbene

(2017) Antonio Tinoco et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway

(2017) Xinna Zhu et al.   METABOLIC ENGINEERING

Whole-cell biocatalysts by design

(2017) Baixue Lin et al.   Microbial Cell Factories

Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

(2017) Christopher K. Prier et al.   Nature Chemistry

The fourth wave of biocatalysis is approaching

(2017) Uwe T. Bornscheuer   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

(2017) Matthew D. Truppo   ACS Medicinal Chemistry Letters

Design of a Pd(0)-CalB CLEA Biohybrid Catalyst and Its Application in a One-Pot Cascade Reaction

(2017) Tamás Görbe et al.   ACS Catalysis

Metrics of Green Chemistry and Sustainability: Past, Present, and Future

(2017) Roger A. Sheldon   ACS Sustainable Chemistry & Engineering

Cell Factory Engineering

(2017) Anne Mathilde Davy et al.   Cell Systems

Gram-Scale Synthesis of Chiral Cyclopropane-Containing Drugs and Drug Precursors with Engineered Myoglobin Catalysts Featuring Complementary Stereoselectivity

(2016) Priyanka Bajaj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A breakthrough in enzyme technology to fight penicillin resistance—industrial application of penicillin amidase

(2016) Klaus Buchholz   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metagenomic mining of glycoside hydrolases from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides ) and the characterization of a multimodular β-1,4-xylanase (GH11)

(2016) Konanani Rashamuse et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

What are the Limitations of Enzymes in Synthetic Organic Chemistry?

(2016) Manfred T. Reetz   CHEMICAL RECORD

Design and engineering of artificial oxygen-activating metalloenzymes

(2016) Flavia Nastri et al.   CHEMICAL SOCIETY REVIEWS

New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes

(2016) Zhoutong Sun et al.   CHEMISTRY-A EUROPEAN JOURNAL

Cover Picture: Asymmetric Catalytic Aza-Diels-Alder/Ring-Closing Cascade Reaction Forming Bicyclic Azaheterocycles by Trienamine Catalysis (Chem. Eur. J. 1/2017)

(2016) Yang Li et al.   CHEMISTRY-A EUROPEAN JOURNAL

Development of a commercial scale process for production of 1,4-butanediol from sugar

(2016) Anthony Burgard et al.   CURRENT OPINION IN BIOTECHNOLOGY

Highly engineered biocatalysts for efficient small molecule pharmaceutical synthesis

(2016) Jim Lalonde   CURRENT OPINION IN BIOTECHNOLOGY

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

(2016) Jochen Wachtmeister et al.   CURRENT OPINION IN BIOTECHNOLOGY

Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

(2016) Elena Fernández-Fueyo et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Abiological catalysis by artificial haem proteins containing noble metals in place of iron

(2016) Hanna M. Key et al.   NATURE

Directed evolution of artificial metalloenzymes for in vivo metathesis

(2016) Markus Jeschek et al.   NATURE

Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines

(2016) Ioannis V. Pavlidis et al.   Nature Chemistry

Directed evolution of cytochrome c for carbon–silicon bond formation: Bringing silicon to life

(2016) S. B. Jennifer Kan et al.   SCIENCE

Biotechnology for Chemical Production: Challenges and Opportunities

(2016) Mark J. Burk et al.   TRENDS IN BIOTECHNOLOGY

Highly Stereoselective Biocatalytic Synthesis of Key Cyclopropane Intermediate to Ticagrelor

(2016) Kari E. Hernandez et al.   ACS Catalysis

Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways

(2016) Scott P. France et al.   ACS Catalysis

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Whole cell biocatalysts: essential workers from Nature to the industry

(2016) Carla C. C. R. de Carvalho   Microbial Biotechnology

Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

(2015) Hans Renata et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Nature-inspired enzymatic cascades to build valuable compounds

(2015) Renata Sigrist et al.   BIOTECHNOLOGY ADVANCES

Redesigning alcohol dehydrogenases/reductases for more efficient biosynthesis of enantiopure isomers

(2015) Rongzhen Zhang et al.   BIOTECHNOLOGY ADVANCES

Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals

(2015) David Jullesson et al.   BIOTECHNOLOGY ADVANCES

Industrial applications of enzyme biocatalysis: Current status and future aspects

(2015) Jung-Min Choi et al.   BIOTECHNOLOGY ADVANCES

Application of Enzyme Coupling Reactions to Shift Thermodynamically Limited Biocatalytic Reactions

(2015) Rohana Abu et al.   ChemCatChem

Imine Reductase-Catalyzed Intermolecular Reductive Amination of Aldehydes and Ketones

(2015) Philipp N. Scheller et al.   ChemCatChem

Artificial concurrent catalytic processes involving enzymes

(2015) Valentin Köhler et al.   CHEMICAL COMMUNICATIONS

Cascade catalysis – strategies and challenges en route to preparative synthetic biology

(2015) Jan Muschiol et al.   CHEMICAL COMMUNICATIONS

Myoglobin-catalyzed intermolecular carbene N–H insertion with arylamine substrates

(2015) Gopeekrishnan Sreenilayam et al.   CHEMICAL COMMUNICATIONS

Recent developments in enzyme promiscuity for carbon–carbon bond-forming reactions

(2015) Yufeng Miao et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The Industrial Age of Biocatalytic Transamination

(2015) Michael Fuchs et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Insights from 20 years of bacterial genome sequencing

(2015) Miriam Land et al.   FUNCTIONAL & INTEGRATIVE GENOMICS

Enzymatic cascade synthesis of (S)-2-hydroxycarboxylic amides and acids: Cascade reactions employing a hydroxynitrile lyase, nitrile-converting enzymes and an amidase

(2015) Fred van Rantwijk et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

(Chemo)enzymatic cascades—Nature's synthetic strategy transferred to the laboratory

(2015) Eduardo García-Junceda et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Chemoenzymatic Dynamic Kinetic Resolution: A Powerful Tool for the Preparation of Enantiomerically Pure Alcohols and Amines

(2015) Oscar Verho et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes To Create New Catalysts

(2015) Christopher K. Prier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Systematic methodology for the development of biocatalytic hydrogen-borrowing cascades: application to the synthesis of chiral α-substituted carboxylic acids from α-substituted α,β-unsaturated aldehydes

(2015) Tanja Knaus et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Development of an Immobilized Ketoreductase for Enzymatic (R)-1-(3,5-Bis(trifluoromethyl)phenyl)ethanol Production

(2015) Hongmei Li et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades

(2015) F. G. Mutti et al.   SCIENCE

Controlling Substrate Specificity and Stereospecificity of Alcohol Dehydrogenases

(2015) Christopher M. Nealon et al.   ACS Catalysis

Toward the Computational Design of Artificial Metalloenzymes: From Protein–Ligand Docking to Multiscale Approaches

(2015) Victor Muñoz Robles et al.   ACS Catalysis

Engineering of Amine Dehydrogenase for Asymmetric Reductive Amination of Ketone by Evolving Rhodococcus Phenylalanine Dehydrogenase

(2015) Li Juan Ye et al.   ACS Catalysis

Enantioselective Enzyme-Catalyzed Aziridination Enabled by Active-Site Evolution of a Cytochrome P450

(2015) Christopher C. Farwell et al.   ACS Central Science

New Generation of Biocatalysts for Organic Synthesis

(2014) Bettina M. Nestl et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Biocompatible Alkene Hydrogenation Merges Organic Synthesis with Microbial Metabolism

(2014) Gopal Sirasani et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Diastereoselective and Enantioselective Olefin Cyclopropanation Using Engineered Myoglobin-Based Catalysts

(2014) Melanie Bordeaux et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzyme Toolbox: Novel Enantiocomplementary Imine Reductases

(2014) Philipp N. Scheller et al.   CHEMBIOCHEM

A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

(2014) K. Engelmark Cassimjee et al.   CHEMICAL COMMUNICATIONS

Enzyme-based inverse opals: a facile and promising platform for fabrication of biocatalysts

(2014) Yanjun Jiang et al.   CHEMICAL COMMUNICATIONS

Magnetic nanomaterials in catalysis: advanced catalysts for magnetic separation and beyond

(2014) Liane M. Rossi et al.   GREEN CHEMISTRY

Combined cross-linked enzyme aggregates (combi-CLEAs) for efficient integration of a ketoreductase and a cofactor regeneration system

(2014) Chenxi Ning et al.   JOURNAL OF BIOTECHNOLOGY

Enantioselective, continuous (R)- and (S)-2-butanol synthesis: Achieving high space-time yields with recombinant E. coli cells in a micro-aqueous, solvent-free reaction system

(2014) Vanessa Erdmann et al.   JOURNAL OF BIOTECHNOLOGY

An integrated biotechnology platform for developing sustainable chemical processes

(2014) Nelson R. Barton et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Chemoenzymatic Dynamic Kinetic Resolution of Primary Amines Using a Recyclable Palladium Nanoparticle Catalyst Together with Lipases

(2014) Karl P. J. Gustafson et al.   JOURNAL OF ORGANIC CHEMISTRY

Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C–H Amination

(2014) Todd K. Hyster et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas

(2014) Oscar Alvizo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biphasic Reaction System Allows for Conversion of Hydrophobic Substrates by Amine Dehydrogenases

(2014) Samantha K. Au et al.   ACS Catalysis

The Evolution of an Amine Dehydrogenase Biocatalyst for the Asymmetric Production of Chiral Amines

(2013) Michael J. Abrahamson et al.   ADVANCED SYNTHESIS & CATALYSIS

Metagenomic mining of feruloyl esterases from termite enteric flora

(2013) Konanani Rashamuse et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Molecular engineering of industrial enzymes: recent advances and future prospects

(2013) Haiquan Yang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells

(2013) Adrie J. J. Straathof   CHEMICAL REVIEWS

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Industrial use of immobilized enzymes

(2013) Robert DiCosimo et al.   CHEMICAL SOCIETY REVIEWS

Whole-cell biocatalysis for selective and productive C–O functional group introduction and modification

(2013) Manfred Schrewe et al.   CHEMICAL SOCIETY REVIEWS

Artificial Multi-Enzyme Networks for the Asymmetric Amination ofsec-Alcohols

(2013) Katharina Tauber et al.   CHEMISTRY-A EUROPEAN JOURNAL

From the first drop to the first truckload: commercialization of microbial processes for renewable chemicals

(2013) Stephen Van Dien   CURRENT OPINION IN BIOTECHNOLOGY

Enzyme catalysed tandem reactions

(2013) Isabel Oroz-Guinea et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

On the development of new biocatalytic processes for practical pharmaceutical synthesis

(2013) Gjalt W Huisman et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

(2013) Manfred T. Reetz   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocatalytic retrosynthesis

(2013) Nicholas J Turner et al.   Nature Chemical Biology

Use of hydrophobic bacterium Rhodococcus rhodochrous NBRC15564 expressed thermophilic alcohol dehydrogenases as whole-cell catalyst in solvent-free organic media

(2013) Aiko Hibino et al.   PROCESS BIOCHEMISTRY

The combi-CLEA approach: enzymatic cascade synthesis of enantiomerically pure (S)-mandelic acid

(2013) Andrzej Chmura et al.   TETRAHEDRON-ASYMMETRY

Recent Developments of Cascade Reactions Involving ω-Transaminases

(2013) Robert C. Simon et al.   ACS Catalysis

Directed evolution: Past, present, and future

(2012) Ryan E. Cobb et al.   AICHE JOURNAL

Development of an Amine Dehydrogenase for Synthesis of Chiral Amines

(2012) Michael J. Abrahamson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Advances in biotechnological production of 1,3-propanediol

(2012) Guneet Kaur et al.   BIOCHEMICAL ENGINEERING JOURNAL

Process technology for multi-enzymatic reaction systems

(2012) Rui Xue et al.   BIORESOURCE TECHNOLOGY

Development of an Immobilized Transaminase Capable of Operating in Organic Solvent

(2012) Matthew D. Truppo et al.   ChemCatChem

Ferritin-supported palladium nanoclusters: selective catalysts for aerobic oxidations in water

(2012) S. Kanbak-Aksu et al.   CHEMICAL COMMUNICATIONS

Protein Engineering of Stereoselective Baeyer-Villiger Monooxygenases

(2012) Zhi-Gang Zhang et al.   CHEMISTRY-A EUROPEAN JOURNAL

Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades

(2012) Jan-Karl Guterl et al.   ChemSusChem

Enzyme-magnetic nanoparticle hybrids: new effective catalysts for the production of high value chemicals

(2012) Humphrey H. P. Yiu et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Synthetic Biology and Metabolic Engineering

(2012) Gregory Stephanopoulos   ACS Synthetic Biology

Multi-Enzymatic Cascade Reactions: Overview and Perspectives

(2011) Emanuele Ricca et al.   ADVANCED SYNTHESIS & CATALYSIS

Industrial biotransformations in the synthesis of building blocks leading to enantiopure drugs

(2011) D. Muñoz Solano et al.   BIORESOURCE TECHNOLOGY

Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates

(2011) Andre Jakoblinnert et al.   CHEMICAL COMMUNICATIONS

New opportunities for biocatalysis: driving the synthesis of chiral chemicals

(2011) Gao-Wei Zheng et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microbial chemical factories: recent advances in pathway engineering for synthesis of value added chemicals

(2011) Himanshu Dhamankar et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Biocatalytic Promiscuity

(2011) Maria Svedendahl Humble et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol

(2011) Harry Yim et al.   Nature Chemical Biology

Dynamic Kinetic Resolution of Amines and Amino Acids by Enzyme-Metal Cocatalysis

(2010) Yunwoong Kim et al.   ChemCatChem

Enzymatic functionalization of carbon–hydrogen bonds

(2010) Jared C. Lewis et al.   CHEMICAL SOCIETY REVIEWS

Practical chiral alcohol manufacture using ketoreductases

(2010) Gjalt W Huisman et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Recent biocatalytic oxidation–reduction cascades

(2010) Joerg H Schrittwieser et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Defining a process operating window for the synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

(2010) Gregory E.R. Gordon et al.   JOURNAL OF BIOTECHNOLOGY

Scale-Up of a Chemo-Biocatalytic Route to (2R,4R)- and (2S,4S)-Monatin

(2010) Amanda L. Rousseau et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

An Integrated Chemo-enzymatic Route for Preparation of β-Thymidine, a Key Intermediate in the Preparation of Antiretrovirals

(2010) Gregory E. R. Gordon et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes

(2010) Pär Tufvesson et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

A novel family VIII carboxylesterase derived from a leachate metagenome library exhibits promiscuous β-lactamase activity on nitrocefin

(2009) Konanani Rashamuse et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Advances in enzyme immobilisation

(2009) Dean Brady et al.   BIOTECHNOLOGY LETTERS

Biocatalysis in development of green pharmaceutical processes

(2009) Junhua Tao et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A green-by-design biocatalytic process for atorvastatin intermediate

(2009) Steven K. Ma et al.   GREEN CHEMISTRY

Efficient Production of Enantiomerically Pure Chiral Amines at Concentrations of 50 g/L Using Transaminases

(2009) Matthew D. Truppo et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Asymmetric Synthesis of Optically Pure Pharmacologically Relevant Amines Employing ω-Transaminases

(2008) Dominik Koszelewski et al.   ADVANCED SYNTHESIS & CATALYSIS

Spherezymes: A novel structured self-immobilisation enzyme technology

(2008) Dean Brady et al.   BMC BIOTECHNOLOGY

Catalytic Promiscuity of Halohydrin Dehalogenase and its Application in Enantioselective Epoxide Ring Opening

(2008) Ghannia Hasnaoui-Dijoux et al.   CHEMBIOCHEM

E factors, green chemistry and catalysis: an odyssey

(2008) Roger A. Sheldon   CHEMICAL COMMUNICATIONS

Biocatalytic promiscuity: the first lipase-catalysed asymmetric aldol reaction

(2008) Chao Li et al.   GREEN CHEMISTRY

New opportunities for biocatalysis: making pharmaceutical processes greener

(2008) John M. Woodley   TRENDS IN BIOTECHNOLOGY

Heterogeneous Raney Nickel and Cobalt Catalysts for Racemization and Dynamic Kinetic Resolution of Amines

(2007) Andrei N. Parvulescu et al.   ADVANCED SYNTHESIS & CATALYSIS