Methods for the directed evolution of proteins

In vivo continuous directed evolution

(2015) Ahmed H Badran et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Flow-Based Enzymatic Ligation by Sortase A

(2014) Rocco L. Policarpo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A high-throughput cellulase screening system based on droplet microfluidics

(2014) Raluca Ostafe et al.   Biomicrofluidics

Accurate design of co-assembling multi-component protein nanomaterials

(2014) Neil P. King et al.   NATURE

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

An orthogonal DNA replication system in yeast

(2014) Arjun Ravikumar et al.   Nature Chemical Biology

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

Directed evolution of a far-red fluorescent rhodopsin

(2014) R. S. McIsaac et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization

(2014) R. Takeuchi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming the specificity of sortase enzymes

(2014) Brent M. Dorr et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes

(2014) J. Shi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computationally designed libraries for rapid enzyme stabilization

(2014) H. J. Wijma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A designed supramolecular protein assembly with in vivo enzymatic activity

(2014) Woon Ju Song et al.   SCIENCE

De novo design of a transmembrane Zn2+-transporting four-helix bundle

(2014) Nathan H. Joh et al.   SCIENCE

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

Engineering V-Type Nerve Agents Detoxifying Enzymes Using Computationally Focused Libraries

(2013) Izhack Cherny et al.   ACS Chemical Biology

Receptor-Directed Chimeric Toxins Created by Sortase-Mediated Protein Fusion

(2013) A. J. McCluskey et al.   MOLECULAR CANCER THERAPEUTICS

Directed evolution of genetic parts and circuits by compartmentalized partnered replication

(2013) Jared W Ellefson et al.   NATURE BIOTECHNOLOGY

Evolution of a designed retro-aldolase leads to complete active site remodeling

(2013) Lars Giger et al.   Nature Chemical Biology

Understanding, predicting and manipulating the genotypic evolution of antibiotic resistance

(2013) Adam C. Palmer et al.   NATURE REVIEWS GENETICS

Rewiring yeast sugar transporter preference through modifying a conserved protein motif

(2013) E. M. Young et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

(2013) L. Yi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

(2013) Zheng Qu et al.   Advanced Healthcare Materials

Directed Evolution of a Model Primordial Enzyme Provides Insights into the Development of the Genetic Code

(2013) Manuel M. Müller et al.   PLoS Genetics

Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiae

(2012) Sun-Mi Lee et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Targeted mutagenesis of the Vibrio fischeri flavin reductase FRase I to improve activation of the anticancer prodrug CB1954

(2012) P.M. Swe et al.   BIOCHEMICAL PHARMACOLOGY

Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells

(2012) Daniel J. Scott et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein Thioester Synthesis Enabled by Sortase

(2012) Jingjing J. Ling et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution of the peroxidase activity of a de novo-designed protein

(2012) S. C. Patel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

A Heritable Recombination System for Synthetic Darwinian Evolution in Yeast

(2012) Dante W. Romanini et al.   ACS Synthetic Biology

Directed Evolution of a Small-Molecule-Triggered Intein with Improved Splicing Properties in Mammalian Cells

(2011) Sun H. Peck et al.   CHEMISTRY & BIOLOGY

Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

(2011) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

Directed Evolution of Sortase A Mutants with Altered Substrate Selectivity Profiles

(2011) Kirill Piotukh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A De Novo Protein Binding Pair By Computational Design and Directed Evolution

(2011) John Karanicolas et al.   MOLECULAR CELL

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

(2011) Rinkoo D Gupta et al.   Nature Chemical Biology

Evolutionary paths to antibiotic resistance under dynamically sustained drug selection

(2011) Erdal Toprak et al.   NATURE GENETICS

Optical recording of action potentials in mammalian neurons using a microbial rhodopsin

(2011) Joel M Kralj et al.   NATURE METHODS

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Directed Evolution of a Protein Container

(2011) B. Worsdorfer et al.   SCIENCE

CyDNA: Synthesis and Replication of Highly Cy-Dye Substituted DNA by an Evolved Polymerase

(2010) Nicola Ramsay et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection

(2010) F. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure-guided reprogramming of serine recombinase DNA sequence specificity

(2010) T. Gaj et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

From wild animals to domestic pets, an evolutionary view of domestication

(2009) C. A. Driscoll et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes

(2008) Stefan Becker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Macromolecular Modeling with Rosetta

(2008) Rhiju Das et al.   Annual Review of Biochemistry

Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli

(2008) S. Atsumi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

High-Throughput Selection for Cellulase Catalysts Using Chemical Complementation

(2008) Pamela Peralta-Yahya et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

Directed enzyme evolution via small and effective neutral drift libraries

(2008) Rinkoo D Gupta et al.   NATURE METHODS

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE