Directed evolution: Past, present, and future

Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

(2012) Yosephine Gumulya et al.   CHEMBIOCHEM

Directed evolution: an evolving and enabling synthetic biology tool

(2012) Ryan E Cobb et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Droplet microfluidics for high-throughput biological assays

(2012) Mira T. Guo et al.   LAB ON A CHIP

Genome-scale promoter engineering by coselection MAGE

(2012) Harris H Wang et al.   NATURE METHODS

Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

(2012) Peter A. Carr et al.   NUCLEIC ACIDS RESEARCH

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Evolutionary Synthetic Biology

(2012) Sergio G. Peisajovich   ACS Synthetic Biology

Multiplexed in Vivo His-Tagging of Enzyme Pathways for in Vitro Single-Pot Multienzyme Catalysis

(2012) Harris H. Wang et al.   ACS Synthetic Biology

Strategy and success for the directed evolution of enzymes

(2011) Paul A Dalby   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineering microbial factories for synthesis of value-added products

(2011) Jing Du et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Lab on a chip technologies for bioenergy and biosustainability research

(2011) Mathias Uhlen et al.   LAB ON A CHIP

A De Novo Protein Binding Pair By Computational Design and Directed Evolution

(2011) John Karanicolas et al.   MOLECULAR CELL

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways

(2011) L. M. Wingler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

An exciting but challenging road ahead for computational enzyme design

(2010) David Baker   PROTEIN SCIENCE

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

Engineering microbial consortia: a new frontier in synthetic biology

(2008) Katie Brenner et al.   TRENDS IN BIOTECHNOLOGY