Chemical proteomics approaches for identifying the cellular targets of natural products

Profiling of Multiple Targets of Artemisinin Activated by Hemin in Cancer Cell Proteome

(2016) Yiqing Zhou et al.   ACS Chemical Biology

Click and release: fluoride cleavable linker for mild bioorthogonal separation

(2016) Elia M. Schneider et al.   CHEMICAL COMMUNICATIONS

Making a Long Journey Short: Alkyne Functionalization of Natural Product Scaffolds

(2016) Johannes Lehmann et al.   CHEMISTRY-A EUROPEAN JOURNAL

Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasitesPlasmodium falciparum3D7

(2016) Hanafy M. Ismail et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In situ Proteomic Profiling of Curcumin Targets in HCT116 Colon Cancer Cell Line

(2016) Jigang Wang et al.   Scientific Reports

Multifunctional Pt(II) Reagents: Covalent Modifications of Pt Complexes Enable Diverse Structural Variation and In-Cell Detection

(2015) Jonathan D. White et al.   ACCOUNTS OF CHEMICAL RESEARCH

Activity-Based Probe for N-Acylethanolamine Acid Amidase

(2015) Elisa Romeo et al.   ACS Chemical Biology

Investigation of Specific Binding Proteins to Photoaffinity Linkers for Efficient Deconvolution of Target Protein

(2015) Jongmin Park et al.   ACS Chemical Biology

Covalent Molecular Probes for Class A G Protein-Coupled Receptors: Advances and Applications

(2015) Dietmar Weichert et al.   ACS Chemical Biology

Quantitative Chemoproteomics for Site-Specific Analysis of Protein Alkylation by 4-Hydroxy-2-Nonenal in Cells

(2015) Jing Yang et al.   ANALYTICAL CHEMISTRY

Multifunctional Reagents for Quantitative Proteome-Wide Analysis of Protein Modification in Human Cells and Dynamic Profiling of Protein Lipidation During Vertebrate Development

(2015) Malgorzata Broncel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Fimbrolide Natural Products Disrupt Bioluminescence of Vibrio By Targeting Autoinducer Biosynthesis and Luciferase Activity

(2015) Weining Zhao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structural, Biochemical, and Computational Studies Reveal the Mechanism of Selective Aldehyde Dehydrogenase 1A1 Inhibition by Cytotoxic Duocarmycin Analogues

(2015) Maximilian F. Koch et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Proteome-Wide Profiling of Targets of Cysteine reactive Small Molecules by Using Ethynyl Benziodoxolone Reagents

(2015) Daniel Abegg et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

ATP Acyl Phosphate Reactivity Reveals Native Conformations of Hsp90 Paralogs and Inhibitor Target Engagement

(2015) Brian E. Nordin et al.   BIOCHEMISTRY

Activity-Based Protein Profiling: Recent Advances in Probe Development and Applications

(2015) Pengyu Yang et al.   CHEMBIOCHEM

Target profiling of zerumbone using a novel cell-permeable clickable probe and quantitative chemical proteomics

(2015) Karunakaran A. Kalesh et al.   CHEMICAL COMMUNICATIONS

Alkynol natural products target ALDH2 in cancer cells by irreversible binding to the active site

(2015) Wolfgang Heydenreuter et al.   CHEMICAL COMMUNICATIONS

Chemoproteomic Evaluation of the Polyacetylene Callyspongynic Acid

(2015) Sabrina Nickel et al.   CHEMISTRY-A EUROPEAN JOURNAL

Covalent protein modification: the current landscape of residue-specific electrophiles

(2015) D Alexander Shannon et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Photoaffinity labeling in target- and binding-site identification

(2015) Ewan Smith et al.   Future Medicinal Chemistry

A Caged Electrophilic Probe for Global Analysis of Cysteine Reactivity in Living Cells

(2015) Masahiro Abo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Global Profiling of Huntingtin-associated protein E (HYPE)-Mediated AMPylation through a Chemical Proteomic Approach

(2015) Malgorzata Broncel et al.   MOLECULAR & CELLULAR PROTEOMICS

Target discovery of acivicin in cancer cells elucidates its mechanism of growth inhibition

(2015) Johannes Kreuzer et al.   Chemical Science

Haem-activated promiscuous targeting of artemisinin in Plasmodium falciparum

(2015) Jigang Wang et al.   Nature Communications

Mapping sites of aspirin-induced acetylations in live cells by quantitative acid-cleavable activity-based protein profiling (QA-ABPP)

(2015) Jigang Wang et al.   Scientific Reports

In situ imaging and proteome profiling indicate andrographolide is a highly promiscuous compound

(2015) Lin Li et al.   Scientific Reports

Clickable Analogue of Cerulenin as Chemical Probe to Explore Protein Palmitoylation

(2014) Baohui Zheng et al.   ACS Chemical Biology

Finding the Right (Bioorthogonal) Chemistry

(2014) David M. Patterson et al.   ACS Chemical Biology

In cell scalaradial interactome profiling using a bio-orthogonal clickable probe

(2014) C. Cassiano et al.   CHEMICAL COMMUNICATIONS

A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins

(2014) Christian Sibbersen et al.   CHEMICAL COMMUNICATIONS

Toll-like Receptors as a Target of Food-derived Anti-inflammatory Compounds

(2014) Takahiro Shibata et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Remodeling Natural Products: Chemistry and Serine Hydrolase Activity of a Rocaglate-Derived β-Lactone

(2014) Neil J. Lajkiewicz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Quantitative Chemical Proteomics Approach to Profile the Specific Cellular Targets of Andrographolide, a Promising Anticancer Agent That Suppresses Tumor Metastasis

(2014) Jigang Wang et al.   MOLECULAR & CELLULAR PROTEOMICS

A road map to evaluate the proteome-wide selectivity of covalent kinase inhibitors

(2014) Bryan R Lanning et al.   Nature Chemical Biology

Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

(2014) Felix Meissner et al.   NATURE IMMUNOLOGY

Seriniquinone, a selective anticancer agent, induces cell death by autophagocytosis, targeting the cancer-protective protein dermcidin

(2014) L. Trzoss et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tracking cancer drugs in living cells by thermal profiling of the proteome

(2014) M. M. Savitski et al.   SCIENCE

Omuralide and Vibralactone: Differences in the Proteasome- β-Lactone-γ-Lactam Binding Scaffold Alter Target Preferences

(2013) Anja List et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Two Faces of Potent Antitumor Duocarmycin-Based Drugs: A Structural Dissection Reveals Disparate Motifs for DNA versus Aldehyde Dehydrogenase 1 Affinity

(2013) Tanja Wirth et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Design and Synthesis of Minimalist Terminal Alkyne-Containing Diazirine Photo-Crosslinkers and Their Incorporation into Kinase Inhibitors for Cell- and Tissue-Based Proteome Profiling

(2013) Zhengqiu Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

(2013) Slava Ziegler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Copper-Assisted Click Reactions for Activity-Based Proteomics: Fine-Tuned Ligands and Refined Conditions Extend the Scope of Application

(2013) Georg C. Rudolf et al.   CHEMBIOCHEM

Species selective diazirine positioning in tag-free photoactive quorum sensing probes

(2013) Luba Dubinsky et al.   CHEMICAL COMMUNICATIONS

Photo-induced covalent cross-linking for the analysis of biomolecular interactions

(2013) George W. Preston et al.   CHEMICAL SOCIETY REVIEWS

Spirohexenolide A Targets Human Macrophage Migration Inhibitory Factor (hMIF)

(2013) Wei-Lun Yu et al.   JOURNAL OF NATURAL PRODUCTS

An Alkyne–Aspirin Chemical Reporter for the Detection of Aspirin-Dependent Protein Modification in Living Cells

(2013) Leslie A. Bateman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Picazoplatin, an Azide-Containing Platinum(II) Derivative for Target Analysis by Click Chemistry

(2013) Jonathan D. White et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases

(2013) Reggy Ekkebus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Targeting Lipid Esterases in Mycobacteria Grown Under Different Physiological Conditions Using Activity-based Profiling with Tetrahydrolipstatin (THL)

(2013) Madhu Sudhan Ravindran et al.   MOLECULAR & CELLULAR PROTEOMICS

Simultaneous structure–activity studies and arming of natural products by C–H amination reveal cellular targets of eupalmerin acetate

(2013) Jing Li et al.   Nature Chemistry

A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles

(2013) Chu Wang et al.   NATURE METHODS

Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells

(2013) Jonathan J Hulce et al.   NATURE METHODS

The CRAPome: a contaminant repository for affinity purification–mass spectrometry data

(2013) Dattatreya Mellacheruvu et al.   NATURE METHODS

Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions

(2013) H. M. Ismail et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay

(2013) D. M. Molina et al.   SCIENCE

Selective Penicillin-Binding Protein Imaging Probes Reveal Substructure in Bacterial Cell Division

(2012) Ozden Kocaoglu et al.   ACS Chemical Biology

Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present

(2012) Marcus Bantscheff et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Activity-Based Probes for Studying the Activity of Flavin-Dependent Oxidases and for the Protein Target Profiling of Monoamine Oxidase Inhibitors

(2012) Joanna M. Krysiak et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Duocarmycin Analogues Target Aldehyde Dehydrogenase 1 in Lung Cancer Cells

(2012) Tanja Wirth et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rugulactone and its Analogues Exert Antibacterial Effects through Multiple Mechanisms Including Inhibition of Thiamine Biosynthesis

(2012) Matthew B. Nodwell et al.   CHEMBIOCHEM

Disulfide- and terminal alkyne-functionalized magnetic silica particles for enrichment of azido glycopeptides

(2012) Sheng Wang et al.   CHEMICAL COMMUNICATIONS

The Antimalarial Natural Product Symplostatin 4 Is a Nanomolar Inhibitor of the Food Vacuole Falcipains

(2012) Sara Christina Stolze et al.   CHEMISTRY & BIOLOGY

How Chemoproteomics Can Enable Drug Discovery and Development

(2012) Raymond E. Moellering et al.   CHEMISTRY & BIOLOGY

Tools for Label-free Peptide Quantification

(2012) Sven Nahnsen et al.   MOLECULAR & CELLULAR PROTEOMICS

A Simple and Effective Cleavable Linker for Chemical Proteomics Applications

(2012) Yinliang Yang et al.   MOLECULAR & CELLULAR PROTEOMICS

Pretubulysin derived probes as novel tools for monitoring the microtubule network via activity-based protein profiling and fluorescence microscopy

(2012) Jürgen Eirich et al.   Molecular BioSystems

Target profiling of 4-hydroxyderricin in S. aureus reveals seryl-tRNA synthetase binding and inhibition by covalent modification

(2012) Oliver A. Battenberg et al.   Molecular BioSystems

Electrophilic natural products and their biological targets

(2012) Malte Gersch et al.   NATURAL PRODUCT REPORTS

Antibiotic activity and target discovery of three-membered natural product-derived heterocycles in pathogenic bacteria

(2012) Maximilian Pitscheider et al.   Chemical Science

Vibralactone as a Tool to Study the Activity and Structure of the ClpP1P2 Complex from Listeria monocytogenes

(2011) Evelyn Zeiler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Diazirine based photoaffinity labeling

(2011) Luba Dubinsky et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe

(2011) Haibin Shi et al.   CHEMICAL COMMUNICATIONS

Unraveling the Protein Targets of Vancomycin in Living S. aureus and E. faecalis Cells

(2011) Jürgen Eirich et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics

(2011) Tamar Geiger et al.   Nature Protocols

Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry

(2011) William P Heal et al.   Nature Protocols

Trends in the exploitation of novel drug targets

(2011) Mathias Rask-Andersen et al.   NATURE REVIEWS DRUG DISCOVERY

Glycosidic Prodrugs of Highly Potent Bifunctional Duocarmycin Derivatives for Selective Treatment of Cancer

(2010) Lutz F. Tietze et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Natural Products and Their Biological Targets: Proteomic and Metabolomic Labeling Strategies

(2010) Thomas Böttcher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Falcarinol is a covalent cannabinoid CB1 receptor antagonist and induces pro-allergic effects in skin

(2010) Marco Leonti et al.   BIOCHEMICAL PHARMACOLOGY

The biological targets of acivicin inspired 3-chloro- and 3-bromodihydroisoxazole scaffolds

(2010) Ronald Orth et al.   CHEMICAL COMMUNICATIONS

Perception and Degradation ofN-Acyl Homoserine Lactone Quorum Sensing Signals by Mammalian and Plant Cells

(2010) Max Teplitski et al.   CHEMICAL REVIEWS

Comparative Analysis of Cleavable Azobenzene-Based Affinity Tags for Bioorthogonal Chemical Proteomics

(2010) Yu-Ying Yang et al.   CHEMISTRY & BIOLOGY

An Optimized Immunoaffinity Fluorescent Method for Natural Product Target Elucidation

(2010) Wei-luen Yu et al.   JOURNAL OF NATURAL PRODUCTS

Showdomycin as a Versatile Chemical Tool for the Detection of Pathogenesis-Associated Enzymes in Bacteria

(2010) Thomas Böttcher et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Natural product mode of action (MOA) studies: a link between natural and synthetic worlds

(2010) James J. La Clair   NATURAL PRODUCT REPORTS

Quantitative reactivity profiling predicts functional cysteines in proteomes

(2010) Eranthie Weerapana et al.   NATURE

Peptidomimetic Antibiotics Target Outer-Membrane Biogenesis in Pseudomonas aeruginosa

(2010) N. Srinivas et al.   SCIENCE

Selective Enrichment of Azide-Containing Peptides from Complex Mixtures

(2009) Merel A. Nessen et al.   JOURNAL OF PROTEOME RESEARCH

Comprehensive Identification of Staurosporine-Binding Kinases in the Hepatocyte Cell Line HepG2 using Capture Compound Mass Spectrometry (CCMS)

(2009) Jenny J. Fischer et al.   JOURNAL OF PROTEOME RESEARCH

A Suite of Activity-Based Probes for Human Cytochrome P450 Enzymes

(2009) Aaron T. Wright et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

β-Lactam Probes As Selective Chemical-Proteomic Tools for the Identification and Functional Characterization of Resistance Associated Enzymes in MRSA

(2009) Isabell Staub et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Robust Fluorescent Detection of Protein Fatty-Acylation with Chemical Reporters

(2009) Guillaume Charron et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Activity-Based Proteome Profiling of Potential Cellular Targets of Orlistat − An FDA-Approved Drug with Anti-Tumor Activities

(2009) Peng-Yu Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Identification of selective inhibitors of uncharacterized enzymes by high-throughput screening with fluorescent activity-based probes

(2009) Daniel A Bachovchin et al.   NATURE BIOTECHNOLOGY

Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics

(2009) Paul J Boersema et al.   Nature Protocols

β-Lactones as Privileged Structures for the Active-Site Labeling of Versatile Bacterial Enzyme Classes

(2008) Thomas Böttcher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Activity-Based Protein Profiling: From Enzyme Chemistry to Proteomic Chemistry

(2008) Benjamin F. Cravatt et al.   Annual Review of Biochemistry

Identification of Protein Targets of 4-Hydroxynonenal Using Click Chemistry for ex Vivo Biotinylation of Azido and Alkynyl Derivatives

(2008) Andrew Vila et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Activity-based probes as a tool for functional proteomic analysis of proteases

(2008) Marko Fonović et al.   Expert Review of Proteomics

β-Lactams as Selective Chemical Probes for the in Vivo Labeling of Bacterial Enzymes Involved in Cell Wall Biosynthesis, Antibiotic Resistance, and Virulence

(2008) Isabell Staub et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Disparate proteome reactivity profiles of carbon electrophiles

(2008) Eranthie Weerapana et al.   Nature Chemical Biology