Advanced in vitro models: Microglia in action

Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids

(2022) Bilal Cakir et al.   Nature Communications

PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids

(2022) Nobuhiko Shiraki et al.   Stem Cell Reports

Microglia and Neurodevelopmental Disorders

(2022) John R. Lukens et al.   Annual Review of Neuroscience

Brain-on-a-chip: Recent advances in design and techniques for microfluidic models of the brain in health and disease

(2022) Leyla Amirifar et al.   BIOMATERIALS

Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality

(2022) Sonia Sabate‐Soler et al.   GLIA

Generation of vascularized brain organoids to study neurovascular interactions

(2022) Xin-Yao Sun et al.   eLife

Minimizing the Ex Vivo Confounds of Cell-Isolation Techniques on Transcriptomic and Translatomic Profiles of Purified Microglia

(2022) Sarah R. Ocañas et al.   eNeuro

Cholesterol and matrisome pathways dysregulated in astrocytes and microglia

(2022) Julia TCW et al.   CELL

Lipid accumulation induced by APOE4 impairs microglial surveillance of neuronal-network activity

(2022) Matheus B. Victor et al.   Cell Stem Cell

Type-I-interferon signaling drives microglial dysfunction and senescence in human iPSC models of Down syndrome and Alzheimer’s disease

(2022) Mengmeng Jin et al.   Cell Stem Cell

A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states

(2022) Nina M. Dräger et al.   NATURE NEUROSCIENCE

Getting the right cells

(2022) Bilal Cakir et al.   eLife

Microglia and Central Nervous System–Associated Macrophages—From Origin to Disease Modulation

(2021) Marco Prinz et al.   Annual Review of Immunology

Regional specification and complementation with non-neuroectodermal cells in human brain organoids

(2021) Yoshiaki Tanaka et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer’s disease

(2021) Sudha R. Guttikonda et al.   NATURE NEUROSCIENCE

Barcoded viral tracing of single-cell interactions in central nervous system inflammation

(2021) Iain C. Clark et al.   SCIENCE

Rapid and Efficient Generation of Myelinating Human Oligodendrocytes in Organoids

(2021) Mohammed R. Shaker et al.   Frontiers in Cellular Neuroscience

Efficient conversion of human induced pluripotent stem cells into microglia by defined transcription factors

(2021) Shih-Wei Chen et al.   Stem Cell Reports

Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

(2021) Galina Popova et al.   Cell Stem Cell

Current tools to interrogate microglial biology

(2021) Anaelle Aurelie Dumas et al.   NEURON

Developing human pluripotent stem cell-based cerebral organoids with a controllable microglia ratio for modeling brain development and pathology

(2021) Ranjie Xu et al.   Stem Cell Reports

Dissecting the complexities of Alzheimer disease with in vitro models of the human brain

(2021) Joel W. Blanchard et al.   Nature Reviews Neurology

Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids

(2021) Ilkka Fagerlund et al.   Cells

Functional and Metabolic Characterization of Microglia Culture in a Defined Medium

(2020) Alejandro Montilla et al.   Frontiers in Cellular Neuroscience

Synthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain

(2020) Yoshiaki Tanaka et al.   Cell Reports

A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation

(2020) Noah Goshi et al.   Journal of Neuroinflammation

A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture

(2020) Alexandra Grubman et al.   Stem Cell Reports

Novel Hexb-based tools for studying microglia in the CNS

(2020) Takahiro Masuda et al.   NATURE IMMUNOLOGY

Deconstructing and reconstructing the human brain with regionally specified brain organoids

(2020) Yangfei Xiang et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

To Kill a Microglia: A Case for CSF1R Inhibitors

(2020) Kim N. Green et al.   TRENDS IN IMMUNOLOGY

Neurodevelopmental Disorders: From Genetics to Functional Pathways

(2020) Ilaria Parenti et al.   TRENDS IN NEUROSCIENCES

SARS-CoV-2 infects the brain choroid plexus and disrupts the blood-CSF-barrier in human brain organoids

(2020) Laura Pellegrini et al.   Cell Stem Cell

Construction of a 3D brain extracellular matrix model to study the interaction between microglia and T cells in co‐culture

(2020) Marie Frühauf et al.   EUROPEAN JOURNAL OF NEUROSCIENCE

Upgrading the Physiological Relevance of Human Brain Organoids

(2020) Ashley Del Dosso et al.   NEURON

Neuron-Glia Interactions in Neurodevelopmental Disorders

(2020) Yoo Sung Kim et al.   Cells

Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

(2020) Yuki Miura et al.   NATURE BIOTECHNOLOGY

Generation of pure monocultures of human microglia-like cells from induced pluripotent stem cells

(2020) Poulomi Banerjee et al.   Stem Cell Research

Organotypic Brain Slice Culture Microglia Exhibit Molecular Similarity to Acutely-Isolated Adult Microglia and Provide a Platform to Study Neuroinflammation

(2020) Alex R. D. Delbridge et al.   Frontiers in Cellular Neuroscience

Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning

(2019) Carl M. Sellgren et al.   NATURE NEUROSCIENCE

hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

(2019) Yangfei Xiang et al.   Cell Stem Cell

Brain organoids: advances, applications and challenges

(2019) Xuyu Qian et al.   DEVELOPMENT

CD22 blockade restores homeostatic microglial phagocytosis in ageing brains

(2019) John V. Pluvinage et al.   NATURE

Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins

(2019) Hyosung Kim et al.   Stem Cell Reports

Targeting microglia in brain disorders

(2019) Josef Priller et al.   SCIENCE

Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells

(2019) Liqing Song et al.   Scientific Reports

Murine iPSC-derived microglia and macrophage cell culture models recapitulate distinct phenotypical and functional properties of classical and alternative neuro-immune polarisation

(2019) Alessandra Quarta et al.   BRAIN BEHAVIOR AND IMMUNITY

Microglia Biology: One Century of Evolving Concepts

(2019) Marco Prinz et al.   CELL

Engineering of human brain organoids with a functional vascular-like system

(2019) Bilal Cakir et al.   NATURE METHODS

Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids

(2019) Julie Bejoy et al.   Stem Cells International

PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia

(2019) Henna Konttinen et al.   Stem Cell Reports

Re-evaluating microglia expression profiles using RiboTag and cell isolation strategies

(2018) Zhana Haimon et al.   NATURE IMMUNOLOGY

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

(2018) Yuan-Ta Lin et al.   NEURON

Generation of human vascularized brain organoids

(2018) Missy T. Pham et al.   NEUROREPORT

Human induced pluripotent stem cell-derived glial cells and neural progenitors display divergent responses to Zika and dengue infections

(2018) Julien Muffat et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia

(2018) Philip W. Brownjohn et al.   Stem Cell Reports

Sorting cells alters their redox state and cellular metabolome

(2018) Elizabeth M. Llufrio et al.   Redox Biology

Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells

(2018) Takenori Ogura et al.   DEVELOPMENT

Induction of myelinating oligodendrocytes in human cortical spheroids

(2018) Mayur Madhavan et al.   NATURE METHODS

Microglia in neurodegeneration

(2018) Suzanne Hickman et al.   NATURE NEUROSCIENCE

An Overview of in vitro Methods to Study Microglia

(2018) Raissa Timmerman et al.   Frontiers in Cellular Neuroscience

Bidirectional Microglia–Neuron Communication in Health and Disease

(2018) Zsuzsanna Szepesi et al.   Frontiers in Cellular Neuroscience

Development and validation of a simplified method to generate human microglia from pluripotent stem cells

(2018) Amanda McQuade et al.   Molecular Neurodegeneration

Microglia innately develop within cerebral organoids

(2018) Paul R. Ormel et al.   Nature Communications

Microglia Increase Inflammatory Responses in iPSC-Derived Human BrainSpheres

(2018) Celina Monteiro Abreu et al.   Frontiers in Microbiology

Organotypic cultures of cerebellar slices as a model to investigate demyelinating disorders

(2017) Frédéric Doussau et al.   Expert Opinion on Drug Discovery

Myeloid Cells in the Central Nervous System

(2017) Jasmin Herz et al.   IMMUNITY

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

(2017) Thais F Galatro et al.   NATURE NEUROSCIENCE

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells

(2017) Hetal Pandya et al.   NATURE NEUROSCIENCE

The use of brain organoids to investigate neural development and disease

(2017) Elizabeth Di Lullo et al.   NATURE REVIEWS NEUROSCIENCE

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases

(2017) Edsel M. Abud et al.   NEURON

Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells

(2017) Steven A. Sloan et al.   NEURON

Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures

(2017) Christopher J. Bohlen et al.   NEURON

An environment-dependent transcriptional network specifies human microglia identity

(2017) David Gosselin et al.   SCIENCE

Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine

(2017) Alexander P. Haring et al.   Stem Cell Reviews and Reports

Earliest accumulation of β-amyloid occurs within the default-mode network and concurrently affects brain connectivity

(2017) Sebastian Palmqvist et al.   Nature Communications

Coupled Proliferation and Apoptosis Maintain the Rapid Turnover of Microglia in the Adult Brain

(2017) Katharine Askew et al.   Cell Reports

Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine

(2017) Alexander P. Haring et al.   Stem Cell Reviews and Reports

Directed Differentiation of Human Pluripotent Stem Cells to Microglia

(2017) Panagiotis Douvaras et al.   Stem Cell Reports

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

(2017) Walther Haenseler et al.   Stem Cell Reports

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Stem Cell Models of Human Brain Development

(2016) Iva Kelava et al.   Cell Stem Cell

The force awakens: insights into the origin and formation of microglia

(2016) Tuan Leng Tay et al.   CURRENT OPINION IN NEUROBIOLOGY

Transcriptome‐based profiling of yolk sac‐derived macrophages reveals a role for Irf8 in macrophage maturation

(2016) Nora Hagemeyer et al.   EMBO JOURNAL

Characterizing primary human microglia: A comparative study with myeloid subsets and culture models

(2016) J. Melief et al.   GLIA

Transcriptome sequencing reveals that LPS-triggered transcriptional responses in established microglia BV2 cell lines are poorly representative of primary microglia

(2016) Amitabh Das et al.   Journal of Neuroinflammation

Microglia across the lifespan: from origin to function in brain development, plasticity and cognition

(2016) Tuan Leng Tay et al.   JOURNAL OF PHYSIOLOGY-LONDON

The promises and challenges of human brain organoids as models of neuropsychiatric disease

(2016) Giorgia Quadrato et al.   NATURE MEDICINE

Efficient derivation of microglia-like cells from human pluripotent stem cells

(2016) Julien Muffat et al.   NATURE MEDICINE

A polarizing question: do M1 and M2 microglia exist?

(2016) Richard M Ransohoff   NATURE NEUROSCIENCE

New tools for studying microglia in the mouse and human CNS

(2016) Mariko L. Bennett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microglia development follows a stepwise program to regulate brain homeostasis

(2016) O. Matcovitch-Natan et al.   SCIENCE

Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells

(2016) Chikafumi Ozone et al.   Nature Communications

Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

(2015) Jin Xu et al.   DEVELOPMENTAL CELL

Mouse models rarely mimic the transcriptome of human neurodegenerative diseases: A systematic bioinformatics-based critique of preclinical models

(2015) Terry C. Burns et al.   EUROPEAN JOURNAL OF PHARMACOLOGY

Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

(2015) Anca M Paşca et al.   NATURE METHODS

Human pluripotent stem cell-derived neural constructs for predicting neural toxicity

(2015) Michael P. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Origin of Microglia: Current Concepts and Past Controversies

(2015) Florent Ginhoux et al.   Cold Spring Harbor Perspectives in Biology

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

(2015) Hideya Sakaguchi et al.   Nature Communications

Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

(2015) Keiko Muguruma et al.   Cell Reports

Nitric Oxide Prevents Aortic Neointimal Hyperplasia by Controlling Macrophage Polarization

(2014) Begoña Lavin et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

CellNet: Network Biology Applied to Stem Cell Engineering

(2014) Patrick Cahan et al.   CELL

An organotypic brain slice preparation from adult patients with temporal lobe epilepsy

(2014) Emmanuel Eugène et al.   JOURNAL OF NEUROSCIENCE METHODS

Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors

(2014) Elisa Gomez Perdiguero et al.   NATURE

Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior

(2014) Yang Zhan et al.   NATURE NEUROSCIENCE

Colony-Stimulating Factor 1 Receptor Signaling Is Necessary for Microglia Viability, Unmasking a Microglia Progenitor Cell in the Adult Brain

(2014) Monica R.P. Elmore et al.   NEURON

The human side of microglia

(2014) Amy M. Smith et al.   TRENDS IN NEUROSCIENCES

Dendritic spine dysgenesis in Rett syndrome

(2014) Xin Xu et al.   Frontiers in Neuroanatomy

Tissue-Resident Macrophages Self-Maintain Locally throughout Adult Life with Minimal Contribution from Circulating Monocytes

(2013) Daigo Hashimoto et al.   IMMUNITY

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent pathways

(2013) Katrin Kierdorf et al.   NATURE NEUROSCIENCE

Identification of a unique TGF-β–dependent molecular and functional signature in microglia

(2013) Oleg Butovsky et al.   NATURE NEUROSCIENCE

The microglial sensome revealed by direct RNA sequencing

(2013) Suzanne E Hickman et al.   NATURE NEUROSCIENCE

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex

(2013) T. Kadoshima et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microfluidic Chemotaxis Platform for Differentiating the Roles of Soluble and Bound Amyloid-β on Microglial Accumulation

(2013) Hansang Cho et al.   Scientific Reports

Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors

(2012) Dong Wook Han et al.   Cell Stem Cell

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Efficient isolation of live microglia with preserved phenotypes from adult mouse brain

(2012) Maria Nikodemova et al.   Journal of Neuroinflammation

Toll/Interleukin-1 Receptor Domain-Containing Adapter Inducing Interferon-  Mediates Microglial Phagocytosis of Degenerating Axons

(2012) S. Hosmane et al.   JOURNAL OF NEUROSCIENCE

A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells

(2012) C. Schulz et al.   SCIENCE

Direct Lineage Conversion of Terminally Differentiated Hepatocytes to Functional Neurons

(2011) Samuele Marro et al.   Cell Stem Cell

In vitro modeling of central nervous system myelination and remyelination

(2011) Andrew A. Jarjour et al.   GLIA

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Infiltrating monocytes trigger EAE progression, but do not contribute to the resident microglia pool

(2011) Bahareh Ajami et al.   NATURE NEUROSCIENCE

Physiology of Microglia

(2011) Helmut Kettenmann et al.   PHYSIOLOGICAL REVIEWS

Synaptic Pruning by Microglia Is Necessary for Normal Brain Development

(2011) R. C. Paolicelli et al.   SCIENCE

The role of microglia in amyloid clearance from the AD brain

(2010) C. Y. Daniel Lee et al.   JOURNAL OF NEURAL TRANSMISSION

A simple magnetic separation method for high-yield isolation of pure primary microglia

(2010) Richard Gordon et al.   JOURNAL OF NEUROSCIENCE METHODS

Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages

(2010) F. Ginhoux et al.   SCIENCE

Substantial early, but nonprogressive neuronal loss in multiple sclerosis (ms) spinal cord

(2009) Lucas Schirmer et al.   ANNALS OF NEUROLOGY

Microglial precursors derived from mouse embryonic stem cells

(2009) Isabella Napoli et al.   GLIA

The Parkinson chimera

(2009) A. J. Lees   NEUROLOGY

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures

(2008) Ryan J. Horvath et al.   JOURNAL OF NEUROCHEMISTRY

Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation

(2008) T. Wataya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA