Brain organoids: advances, applications and challenges

Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation

(2018) Ikuo K. Suzuki et al.   CELL

Human-Specific NOTCH2NL Genes Affect Notch Signaling and Cortical Neurogenesis

(2018) Ian T. Fiddes et al.   CELL

Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses

(2018) Uthra Rajamani et al.   Cell Stem Cell

hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate

(2018) M. Zeeshan Ozair et al.   Cell Stem Cell

A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex

(2018) Suijuan Zhong et al.   NATURE

An in vivo model of functional and vascularized human brain organoids

(2018) Abed AlFatah Mansour et al.   NATURE BIOTECHNOLOGY

Common schizophrenia alleles are enriched in mutation-intolerant genes and in regions under strong background selection

(2018) Antonio F. Pardiñas et al.   NATURE GENETICS

Genetically engineered cerebral organoids model brain tumor formation

(2018) Shan Bian et al.   NATURE METHODS

Human brain organoids on a chip reveal the physics of folding

(2018) Eyal Karzbrun et al.   Nature Physics

Generation of human brain region–specific organoids using a miniaturized spinning bioreactor

(2018) Xuyu Qian et al.   Nature Protocols

Generation of human vascularized brain organoids

(2018) Missy T. Pham et al.   NEUROREPORT

High-resolution comparative analysis of great ape genomes

(2018) Zev N. Kronenberg et al.   SCIENCE

Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling

(2018) Goodwell Nzou et al.   Scientific Reports

Human Neurospheroid Arrays for In Vitro Studies of Alzheimer’s Disease

(2018) Mehdi Jorfi et al.   Scientific Reports

Glioblastoma Model Using Human Cerebral Organoids

(2018) Junko Ogawa et al.   Cell Reports

Exploring the Complexity of Cortical Development Using Single-Cell Transcriptomics

(2018) Hyobin Jeong et al.   Frontiers in Neuroscience

Applications of human brain organoids to clinical problems

(2018) H. Isaac Chen et al.   DEVELOPMENTAL DYNAMICS

Modeling amyloid beta and tau pathology in human cerebral organoids

(2018) Cesar Gonzalez et al.   MOLECULAR PSYCHIATRY

Induction of myelinating oligodendrocytes in human cortical spheroids

(2018) Mayur Madhavan et al.   NATURE METHODS

Exploring landscapes of brain morphogenesis with organoids

(2018) Denis Jabaudon et al.   DEVELOPMENT

Reliability of human cortical organoid generation

(2018) Se-Jin Yoon et al.   NATURE METHODS

Blood–brain barrier-on-a-chip: Microphysiological systems that capture the complexity of the blood–central nervous system interface

(2017) Duc TT Phan et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

How does Zika virus cause microcephaly?

(2017) Zhexing Wen et al.   GENES & DEVELOPMENT

Assembly of functionally integrated human forebrain spheroids

(2017) Fikri Birey et al.   NATURE

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Guided self-organization and cortical plate formation in human brain organoids

(2017) Madeline A Lancaster et al.   NATURE BIOTECHNOLOGY

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Fused cerebral organoids model interactions between brain regions

(2017) Joshua A Bagley et al.   NATURE METHODS

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases

(2017) Edsel M. Abud et al.   NEURON

Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells

(2017) Steven A. Sloan et al.   NEURON

DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis

(2017) Fei Ye et al.   NEURON

Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex

(2017) Tomasz J. Nowakowski et al.   SCIENCE

A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity

(2017) David Pamies   ALTEX-Alternatives to Animal Experimentation

Dynamic behaviour of human neuroepithelial cells in the developing forebrain

(2017) Lakshmi Subramanian et al.   Nature Communications

Stem cell models of Alzheimer’s disease: progress and challenges

(2017) Charles Arber et al.   Alzheimers Research & Therapy

Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection

(2017) Momoko Watanabe et al.   Cell Reports

An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome

(2017) Vira Iefremova et al.   Cell Reports

Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease

(2017) Rui Li et al.   Protein & Cell

Characterizing the Pattern of Anomalies in Congenital Zika Syndrome for Pediatric Clinicians

(2017) Cynthia A. Moore et al.   JAMA Pediatrics

Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells

(2017) Anna S. Monzel et al.   Stem Cell Reports

A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo

(2016) Christopher G. Hubert et al.   CANCER RESEARCH

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Neural stem cells attacked by Zika virus

(2016) Ha Nam Nguyen et al.   CELL RESEARCH

Advances in Zika Virus Research: Stem Cell Models, Challenges, and Opportunities

(2016) Guo-li Ming et al.   Cell Stem Cell

2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size

(2016) Tomoki Otani et al.   Cell Stem Cell

Midbrain-like Organoids from Human Pluripotent Stem Cells Contain Functional Dopaminergic and Neuromelanin-Producing Neurons

(2016) Junghyun Jo et al.   Cell Stem Cell

Zika Virus Depletes Neural Progenitors in Human Cerebral Organoids through Activation of the Innate Immune Receptor TLR3

(2016) Jason Dang et al.   Cell Stem Cell

DMRT5 Together with DMRT3 Directly Controls Hippocampus Development and Neocortical Area Map Formation

(2016) Sarah De Clercq et al.   CEREBRAL CORTEX

CPAP promotes timely cilium disassembly to maintain neural progenitor pool

(2016) Elke Gabriel et al.   EMBO JOURNAL

Comprehensive cellular-resolution atlas of the adult human brain

(2016) Song-Lin Ding et al.   JOURNAL OF COMPARATIVE NEUROLOGY

Induced pluripotent stem cells in Parkinson's disease: scientific and clinical challenges

(2016) Bin Xiao et al.   JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY

Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study

(2016) Guilherme Calvet et al.   LANCET INFECTIOUS DISEASES

Modeling of Autism Using Organoid Technology

(2016) Hwan Choi et al.   MOLECULAR NEUROBIOLOGY

The Brazilian Zika virus strain causes birth defects in experimental models

(2016) Fernanda R. Cugola et al.   NATURE

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

(2016) Miao Xu et al.   NATURE MEDICINE

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

(2016) Michael J Workman et al.   NATURE MEDICINE

Transformation of the Radial Glia Scaffold Demarcates Two Stages of Human Cerebral Cortex Development

(2016) Tomasz J. Nowakowski et al.   NEURON

Zika Virus and Birth Defects — Reviewing the Evidence for Causality

(2016) Sonja A. Rasmussen et al.   NEW ENGLAND JOURNAL OF MEDICINE

Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

(2016) Feiran Zhang et al.   NUCLEIC ACIDS RESEARCH

Comment on "Cortical folding scales universally with surface area and thickness, not number of neurons"

(2016) E. Lewitus et al.   SCIENCE

Zika virus impairs growth in human neurospheres and brain organoids

(2016) P. P. Garcez et al.   SCIENCE

Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells

(2016) Chikafumi Ozone et al.   Nature Communications

Cerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain

(2016) Chongyuan Luo et al.   Cell Reports

Generating Late-Onset Human iPSC-Based Disease Models by Inducing Neuronal Age-Related Phenotypes through Telomerase Manipulation

(2016) Elsa Vera et al.   Cell Reports

Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

(2016) Felipe Mora-Bermúdez et al.   eLife

Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate Alzheimer’s Disease Phenotypes

(2016) Waseem K. Raja et al.   PLoS One

FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders

(2015) Jessica Mariani et al.   CELL

Molecular Identity of Human Outer Radial Glia during Cortical Development

(2015) Alex A. Pollen et al.   CELL

Programming and Reprogramming Cellular Age in the Era of Induced Pluripotency

(2015) Lorenz Studer et al.   Cell Stem Cell

Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

(2015) Anca M Paşca et al.   NATURE METHODS

Postmitotic regulation of sensory area patterning in the mammalian neocortex by Lhx2

(2015) Andreas Zembrzycki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

(2015) J. Gray Camp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cortical folding scales universally with surface area and thickness, not number of neurons

(2015) B. Mota et al.   SCIENCE

Human-specific gene ARHGAP11B promotes basal progenitor amplification and neocortex expansion

(2015) M. Florio et al.   SCIENCE

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

(2015) Hideya Sakaguchi et al.   Nature Communications

Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

(2015) Keiko Muguruma et al.   Cell Reports

The cortical hem regulates the size and patterning of neocortex

(2014) Giuliana Caronia-Brown et al.   DEVELOPMENT

A three-dimensional human neural cell culture model of Alzheimer’s disease

(2014) Se Hoon Choi et al.   NATURE

Generation of cerebral organoids from human pluripotent stem cells

(2014) Madeline A Lancaster et al.   Nature Protocols

Patches of Disorganization in the Neocortex of Children with Autism

(2014) Rich Stoner et al.   NEW ENGLAND JOURNAL OF MEDICINE

Evolutionarily Dynamic Alternative Splicing of GPR56 Regulates Regional Cerebral Cortical Patterning

(2014) B.-I. Bae et al.   SCIENCE

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells

(2014) Masato Nakagawa et al.   Scientific Reports

Diversity of Cortical Interneurons in Primates: The Role of the Dorsal Proliferative Niche

(2014) Nevena V. Radonjić et al.   Cell Reports

3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells

(2014) Andrea Meinhardt et al.   Stem Cell Reports

Trnp1 Regulates Expansion and Folding of the Mammalian Cerebral Cortex by Control of Radial Glial Fate

(2013) Ronny Stahl et al.   CELL

Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging

(2013) Justine D. Miller et al.   Cell Stem Cell

Next-Generation Regenerative Medicine: Organogenesis from Stem Cells in 3D Culture

(2013) Yoshiki Sasai   Cell Stem Cell

Conical expansion of the outer subventricular zone and the role of neocortical folding in evolution and development

(2013) Eric Lewitus et al.   Frontiers in Human Neuroscience

Mechanical forces in cerebral cortical folding: A review of measurements and models

(2013) P.V. Bayly et al.   Journal of the Mechanical Behavior of Biomedical Materials

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Non-epithelial stem cells and cortical interneuron production in the human ganglionic eminences

(2013) David V Hansen et al.   NATURE NEUROSCIENCE

Subcortical origins of human and monkey neocortical interneurons

(2013) Tong Ma et al.   NATURE NEUROSCIENCE

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map

(2013) G. E. Elsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex

(2013) T. Kadoshima et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of cortical folding during evolution and ontogeny

(2013) Karl Zilles et al.   TRENDS IN NEUROSCIENCES

Large-Scale Cellular-Resolution Gene Profiling in Human Neocortex Reveals Species-Specific Molecular Signatures

(2012) Hongkui Zeng et al.   CELL

Development and Evolution of the Human Neocortex

(2011) Jan H. Lui et al.   CELL

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Dorsal Radial Glial Cells Have the Potential to Generate Cortical Interneurons in Human But Not in Mouse Brain

(2011) X. Yu et al.   JOURNAL OF NEUROSCIENCE

Subregional Specification of Embryonic Stem Cell-Derived Ventral Telencephalic Tissues by Timed and Combinatory Treatment with Extrinsic Signals

(2011) T. Danjo et al.   JOURNAL OF NEUROSCIENCE

The Corticofugal Neuron-Associated Genes ROBO1, SRGAP1, and CTIP2 Exhibit an Anterior to Posterior Gradient of Expression in Early Fetal Human Neocortex Development

(2010) Bui Kar Ip et al.   CEREBRAL CORTEX

Multiple Origins of Human Neocortical Interneurons Are Supported by Distinct Expression of Transcription Factors

(2010) Igor Jakovcevski et al.   CEREBRAL CORTEX

Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression

(2010) Takashi Saito et al.   CEREBRAL CORTEX

Neurogenic radial glia in the outer subventricular zone of human neocortex

(2010) David V. Hansen et al.   NATURE

Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo

(2010) Lodovica Borghese et al.   STEM CELLS

Culturing thick brain slices: An interstitial 3D microperfusion system for enhanced viability

(2009) Komal Rambani et al.   JOURNAL OF NEUROSCIENCE METHODS

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Evolution of the neocortex: a perspective from developmental biology

(2009) Pasko Rakic   NATURE REVIEWS NEUROSCIENCE

Decision by division: making cortical maps

(2009) Pasko Rakic et al.   TRENDS IN NEUROSCIENCES

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Origins of Cortical GABAergic Neurons in the Cynomolgus Monkey

(2008) Z. Petanjek et al.   CEREBRAL CORTEX

Development of the human cerebral cortex: Boulder Committee revisited

(2008) Irina Bystron et al.   NATURE REVIEWS NEUROSCIENCE