Rethinking organoid technology through bioengineering

Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids

(2020) Susanne C. van den Brink et al.   NATURE

Human blood vessel organoids as a model of diabetic vasculopathy

(2019) Reiner A. Wimmer et al.   NATURE

Flow-enhanced vascularization and maturation of kidney organoids in vitro

(2019) Kimberly A. Homan et al.   NATURE METHODS

Accelerating maturation of kidney organoids

(2019) Susana M. Chuva de Sousa Lopes   NATURE MATERIALS

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells

(2019) Elena Garreta et al.   NATURE MATERIALS

A single-cell molecular map of mouse gastrulation and early organogenesis

(2019) Blanca Pijuan-Sala et al.   NATURE

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models

(2019) Stefano Vianello et al.   DEVELOPMENTAL CELL

The emergent landscape of the mouse gut endoderm at single-cell resolution

(2019) Sonja Nowotschin et al.   NATURE

Polarizing brain organoids

(2019) Yuki Miura et al.   NATURE BIOTECHNOLOGY

Specification of positional identity in forebrain organoids

(2019) Gustav Y. Cederquist et al.   NATURE BIOTECHNOLOGY

3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts

(2019) Nadav Noor et al.   Advanced Science

Mechanochemical Feedback Loops in Development and Disease

(2019) Edouard Hannezo et al.   CELL

Design Approaches for Generating Organ Constructs

(2019) Yun Xia et al.   Cell Stem Cell

Exploring single cells in space and time during tissue development, homeostasis and regeneration

(2019) Urs Mayr et al.   DEVELOPMENT

A periodic table of cell types

(2019) Bo Xia et al.   DEVELOPMENT

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

(2019) Andrea Manfrin et al.   NATURE METHODS

Genetic induction and mechanochemical propagation of a morphogenetic wave

(2019) Anaïs Bailles et al.   NATURE

Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary

(2019) Hiroyuki Koike et al.   NATURE

Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform

(2019) Kevin Achberger et al.   eLife

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels

(2019) Mark A. Skylar-Scott et al.   Science Advances

Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms

(2018) Thomas A. Forbes et al.   AMERICAN JOURNAL OF HUMAN GENETICS

3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes

(2018) Marco Campisi et al.   BIOMATERIALS

High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

(2018) Stefan M. Czerniecki et al.   Cell Stem Cell

An in vivo model of functional and vascularized human brain organoids

(2018) Abed AlFatah Mansour et al.   NATURE BIOTECHNOLOGY

Genetically engineered cerebral organoids model brain tumor formation

(2018) Shan Bian et al.   NATURE METHODS

Human brain organoids on a chip reveal the physics of folding

(2018) Eyal Karzbrun et al.   Nature Physics

Glioblastoma Model Using Human Cerebral Organoids

(2018) Junko Ogawa et al.   Cell Reports

Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo

(2018) Cathelijne W. van den Berg et al.   Stem Cell Reports

A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells

(2018) Aneta Przepiorski et al.   Stem Cell Reports

Human stomach-on-a-chip with luminal flow and peristaltic-like motility

(2018) Kang Kug Lee et al.   LAB ON A CHIP

Self-organization of a human organizer by combined Wnt and Nodal signalling

(2018) I. Martyn et al.   NATURE

Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells

(2018) Mark F Sabbagh et al.   eLife

Reproducibility of Molecular Phenotypes after Long-Term Differentiation to Human iPSC-Derived Neurons: A Multi-Site Omics Study

(2018) Viola Volpato et al.   Stem Cell Reports

Active superelasticity in three-dimensional epithelia of controlled shape

(2018) Ernest Latorre et al.   NATURE

Evaluation of variability in human kidney organoids

(2018) Belinda Phipson et al.   NATURE METHODS

A 3D microvascular network model to study the impact of hypoxia on the extravasation potential of breast cell lines

(2018) Jiho Song et al.   Scientific Reports

Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons

(2018) Tatsuya Osaki et al.   Science Advances

Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis

(2018) S. Okuda et al.   Science Advances

Assembly of functionally integrated human forebrain spheroids

(2017) Fikri Birey et al.   NATURE

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Guided self-organization and cortical plate formation in human brain organoids

(2017) Madeline A Lancaster et al.   NATURE BIOTECHNOLOGY

Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells

(2017) Karl R Koehler et al.   NATURE BIOTECHNOLOGY

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Quantifying forces in cell biology

(2017) Pere Roca-Cusachs et al.   NATURE CELL BIOLOGY

Vertex models: from cell mechanics to tissue morphogenesis

(2017) Silvanus Alt et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Human tissues in a dish: The research and ethical implications of organoid technology

(2017) Annelien L. Bredenoord et al.   SCIENCE

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

(2017) Takanori Takebe et al.   Cell Reports

Functional coupling of human pancreatic islets and liver spheroids on-a-chip: Towards a novel human ex vivo type 2 diabetes model

(2017) Sophie Bauer et al.   Scientific Reports

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

(2016) Michael J Workman et al.   NATURE MEDICINE

TissueMiner: A multiscale analysis toolkit to quantify how cellular processes create tissue dynamics

(2016) Raphaël Etournay et al.   eLife

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

(2015) Takanori Takebe et al.   Cell Stem Cell

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2015) Minoru Takasato et al.   NATURE

Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

(2015) Anca M Paşca et al.   NATURE METHODS

Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform

(2015) Sebastien G. M. Uzel et al.   Small

Self-organizing human cardiac microchambers mediated by geometric confinement

(2015) Zhen Ma et al.   Nature Communications

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

(2015) Hideya Sakaguchi et al.   Nature Communications

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

(2015) Benjamin S. Freedman et al.   Nature Communications

In vitro generation of human pluripotent stem cell derived lung organoids

(2015) Briana R Dye et al.   eLife

KeyGenes, a Tool to Probe Tissue Differentiation Using a Human Fetal Transcriptional Atlas

(2015) Matthias S. Roost et al.   Stem Cell Reports

Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury

(2014) Mandy B. Esch et al.   LAB ON A CHIP

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells

(2014) Aryeh Warmflash et al.   NATURE METHODS

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells

(2014) Andrea Meinhardt et al.   Stem Cell Reports

Mesenchymal stem cell durotaxis depends on substrate stiffness gradient strength

(2013) Ludovic G. Vincent et al.   Biotechnology Journal

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Quantifying cell-generated mechanical forces within living embryonic tissues

(2013) Otger Campàs et al.   NATURE METHODS

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Stiffening hydrogels to probe short- and long-term cellular responses to dynamic mechanics

(2012) Murat Guvendiren et al.   Nature Communications

Extracellular matrix and cytoskeletal dynamics during branching morphogenesis

(2012) Hye Young Kim et al.   Organogenesis

Photoreversible Patterning of Biomolecules within Click-Based Hydrogels

(2011) Cole A. DeForest et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Measurement of mechanical tractions exerted by cells in three-dimensional matrices

(2010) Wesley R Legant et al.   NATURE METHODS

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies

(2008) Derk ten Berge et al.   Cell Stem Cell

Pattern formation in the vertebrate neural tube: a sonic hedgehog morphogen-regulated transcriptional network

(2008) E. Dessaud et al.   DEVELOPMENT