Radical SAM Enzymes in the Biosynthesis of Ribosomally Synthesized and Post-translationally Modified Peptides (RiPPs)

Mechanistic elucidation of the mycofactocin-biosynthetic radicalS-adenosylmethionine protein, MftC

(2017) Bulat Khaliullin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Insights into the catalysis of a lysine-tryptophan bond in bacterial peptides by a SPASM domain radical S -adenosylmethionine (SAM) peptide cyclase

(2017) Alhosna Benjdia et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Efficient methylation of C2 in l-tryptophan by the cobalamin-dependent radicalS-adenosylmethionine methylase TsrM requires an unmodified N1 amine

(2017) Anthony J. Blaszczyk et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Reconstitution and Substrate Specificity of the Radical S-Adenosyl-methionine Thiazole C-Methyltransferase in Thiomuracin Biosynthesis

(2017) Nilkamal Mahanta et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural Insights into Thioether Bond Formation in the Biosynthesis of Sactipeptides

(2017) Tyler L. Grove et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis

(2017) Alhosna Benjdia et al.   Nature Chemistry

DNA Repair by the Radical SAM Enzyme Spore Photoproduct Lyase: From Biochemistry to Structural Investigations

(2017) Olivier Berteau et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY

Structures of the peptide-modifying radical SAM enzyme SuiB elucidate the basis of substrate recognition

(2017) Katherine M. Davis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of Sactipeptides in Escherichia coli: Probing the Substrate Promiscuity of Subtilosin A Biosynthesis

(2016) Paul M. Himes et al.   ACS Chemical Biology

Mechanistic Insights into the Radical S -adenosyl-l -methionine Enzyme NosL From a Substrate Analogue and the Shunt Products

(2016) Xinjian Ji et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Sulfatases and radical SAM enzymes: emerging themes in glycosaminoglycan metabolism and the human microbiota

(2016) A. Benjdia et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The Radical S-Adenosyl-l-methionine Enzyme MftC Catalyzes an Oxidative Decarboxylation of the C-Terminus of the MftA Peptide

(2016) Nathan A. Bruender et al.   BIOCHEMISTRY

SkfB Abstracts a Hydrogen Atom from Cα on SkfA To Initiate Thioether Cross-Link Formation

(2016) Nathan A. Bruender et al.   BIOCHEMISTRY

Emerging Diversity of the Cobalamin-Dependent Methyltransferases Involving Radical-Based Mechanisms

(2016) Wei Ding et al.   CHEMBIOCHEM

Thioether bond formation by SPASM domain radical SAM enzymes: Cα H-atom abstraction in subtilosin A biosynthesis

(2016) Alhosna Benjdia et al.   CHEMICAL COMMUNICATIONS

Mechanistic insights into class B radical-S-adenosylmethionine methylases: ubiquitous tailoring enzymes in natural product biosynthesis

(2016) Shanshan Zhou et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Mycofactocin biosynthesis: modification of the peptide MftA by the radical S-adenosylmethionine protein MftC

(2016) Bulat Khaliullin et al.   FEBS LETTERS

Demonstration That the RadicalS-Adenosylmethionine (SAM) Enzyme PqqE Catalyzesde NovoCarbon-Carbon Cross-linking within a Peptide Substrate PqqA in the Presence of the Peptide Chaperone PqqD

(2016) Ian Barr et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The B12-Radical SAM Enzyme PoyC Catalyzes Valine Cβ-Methylation during Polytheonamide Biosynthesis

(2016) Aubérie Parent et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tryptophan Lyase (NosL): A Cornucopia of 5′-Deoxyadenosyl Radical Mediated Transformations

(2016) Dhananjay M. Bhandari et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Spectroscopic and Electrochemical Characterization of the Iron–Sulfur and Cobalamin Cofactors of TsrM, an Unusual Radical S-Adenosylmethionine Methylase

(2016) Anthony J. Blaszczyk et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Carbon extension in peptidylnucleoside biosynthesis by radical SAM enzymes

(2016) Edward A Lilla et al.   Nature Chemical Biology

Carbon–sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE

(2016) Roman Rohac et al.   Nature Chemistry

Structural basis for tRNA modification by Elp3 from Dehalococcoides mccartyi

(2016) Sebastian Glatt et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Fine-tuning of a radical-based reaction by radical S-adenosyl-L-methionine tryptophan lyase

(2016) G. Sicoli et al.   SCIENCE

The radical mechanism of biological methane synthesis by methyl-coenzyme M reductase

(2016) T. Wongnate et al.   SCIENCE

Structural basis for tRNA modification by Elp3 from Dehalococcoides mccartyi

(2016) Sebastian Glatt et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Catalytic Promiscuity of the Radical S-adenosyl-L-methionine Enzyme NosL

(2016) Wei Ding et al.   Frontiers in Chemistry

Substrate-Tuned Catalysis of the RadicalS-Adenosyl-L-Methionine Enzyme NosL Involved in Nosiheptide Biosynthesis

(2015) Xinjian Ji et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Tryptophan Lyase (NosL): Mechanistic Insights from Substrate Analogues and Mutagenesis

(2015) Dhananjay M. Bhandari et al.   BIOCHEMISTRY

The RadicalS-Adenosyl-l-methionine Enzyme QhpD Catalyzes Sequential Formation of Intra-protein Sulfur-to-Methylene Carbon Thioether Bonds

(2015) Tadashi Nakai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A prevalent peptide-binding domain guides ribosomal natural product biosynthesis

(2015) Brandon J Burkhart et al.   Nature Chemical Biology

Structure and biosynthesis of a macrocyclic peptide containing an unprecedented lysine-to-tryptophan crosslink

(2015) Kelsey R. Schramma et al.   Nature Chemistry

Consecutive radicalS-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis

(2015) Daniel R. Marous et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The thiostrepton A tryptophan methyltransferase TsrM catalyses a cob(II)alamin-dependent methyl transfer reaction

(2015) Alhosna Benjdia et al.   Nature Communications

RadicalS-Adenosyl Methionine Epimerases: Regioselective Introduction of DiverseD-Amino Acid Patterns into Peptide Natural Products

(2014) Brandon I. Morinaka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Crystal Structure of Tryptophan Lyase (NosL): Evidence for Radical Formation at the Amino Group of Tryptophan

(2014) Yvain Nicolet et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase

(2014) Alhosna Benjdia et al.   CHEMICAL COMMUNICATIONS

Radical S-Adenosylmethionine Enzymes

(2014) Joan B. Broderick et al.   CHEMICAL REVIEWS

SPASM and Twitch Domains inS-Adenosylmethionine (SAM) Radical Enzymes

(2014) Tsehai A. J. Grell et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterization of a Radical S-Adenosyl-l-methionine Epimerase, NeoN, in the Last Step of Neomycin B Biosynthesis

(2014) Fumitaka Kudo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

C–H Methylation of Heteroarenes Inspired by Radical SAM Methyl Transferase

(2014) Jinghan Gui et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An environmental bacterial taxon with a large and distinct metabolic repertoire

(2014) Micheal C. Wilson et al.   NATURE

Substrate control in stereoselective lanthionine biosynthesis

(2014) Weixin Tang et al.   Nature Chemistry

Two [4Fe-4S] Clusters Containing Radical SAM Enzyme SkfB Catalyze Thioether Bond Formation during the Maturation of the Sporulation Killing Factor

(2013) Leif Flühe et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

GenK-Catalyzed C-6′ Methylation in the Biosynthesis of Gentamicin: Isolation and Characterization of a Cobalamin-Dependent Radical SAM Enzyme

(2013) Hak Joong Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification

(2013) P. J. Goldman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Insights into Quinaldic Acid Moiety Formation in Thiostrepton Biosynthesis Facilitating Fluorinated Thiopeptide Generation

(2012) Lian Duan et al.   CHEMISTRY & BIOLOGY

Synthetic Biotechnology to Study and Engineer Ribosomal Bottromycin Biosynthesis

(2012) Liujie Huo et al.   CHEMISTRY & BIOLOGY

DNA photolyases and SP lyase: structure and mechanism of light-dependent and independent DNA lyases

(2012) Alhosna Benjdia   CURRENT OPINION IN STRUCTURAL BIOLOGY

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature

(2012) Paul G. Arnison et al.   NATURAL PRODUCT REPORTS

Visualizing molecular juggling within a B12-dependent methyltransferase complex

(2012) Yan Kung et al.   NATURE

Thiostrepton tryptophan methyltransferase expands the chemistry of radical SAM enzymes

(2012) Stéphane Pierre et al.   Nature Chemical Biology

The radical SAM enzyme AlbA catalyzes thioether bond formation in subtilosin A

(2012) Leif Flühe et al.   Nature Chemical Biology

Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme

(2012) Alhosna Benjdia et al.   NUCLEIC ACIDS RESEARCH

Metagenome Mining Reveals Polytheonamides as Posttranslationally Modified Ribosomal Peptides

(2012) M. F. Freeman et al.   SCIENCE

Radical-Mediated Enzymatic Methylation: A Tale of Two SAMS

(2011) Qi Zhang et al.   ACCOUNTS OF CHEMICAL RESEARCH

The 3D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks

(2011) Clarissa S. Sit et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In Vitro Phosphinate Methylation by PhpK fromKitasatospora phosalacinea

(2011) Williard J. Werner et al.   BIOCHEMISTRY

Biological Systems Discovery In Silico: Radical S-Adenosylmethionine Protein Families and Their Target Peptides for Posttranslational Modification

(2011) D. H. Haft et al.   JOURNAL OF BACTERIOLOGY

Sulfatases and a RadicalS-Adenosyl-l-methionine (AdoMet) Enzyme Are Key for Mucosal Foraging and Fitness of the Prominent Human Gut Symbiont,Bacteroides thetaiotaomicron

(2011) Alhosna Benjdia et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Deficit of tRNALys modification by Cdkal1 causes the development of type 2 diabetes in mice

(2011) Fan-Yan Wei et al.   JOURNAL OF CLINICAL INVESTIGATION

Radical-mediated enzymatic carbon chain fragmentation-recombination

(2011) Qi Zhang et al.   Nature Chemical Biology

Organocatalytic strategies for the asymmetric functionalization of indoles

(2010) Giuseppe Bartoli et al.   CHEMICAL SOCIETY REVIEWS

Enzymatic functionalization of carbon–hydrogen bonds

(2010) Jared C. Lewis et al.   CHEMICAL SOCIETY REVIEWS

Anaerobic sulfatase-maturating enzyme - A mechanistic link with glycyl radical-activating enzymes?

(2010) Alhosna Benjdia et al.   FEBS Journal

Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis

(2010) W.-T. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thuricin CD, a posttranslationally modified bacteriocin with a narrow spectrum of activity against Clostridium difficile

(2010) M. C. Rea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Structural and Biochemical Foundations of Thiamin Biosynthesis

(2009) Christopher T. Jurgenson et al.   Annual Review of Biochemistry

Pyrroloquinoline Quinone Biogenesis: Demonstration That PqqE fromKlebsiella pneumoniaeIs a RadicalS-Adenosyl-l-methionine Enzyme

(2009) Stephen R. Wecksler et al.   BIOCHEMISTRY

Thiopeptide Biosynthesis Featuring Ribosomally Synthesized Precursor Peptides and Conserved Posttranslational Modifications

(2009) Rijing Liao et al.   CHEMISTRY & BIOLOGY

Catalytic Activity of the Anaerobic Tyrosine Lyase Required for Thiamine Biosynthesis in Escherichia coli

(2009) Martin R. Challand et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanistic Investigations of Anaerobic Sulfatase-Maturating Enzyme: Direct CβH-Atom Abstraction Catalyzed by a Radical AdoMet Enzyme

(2009) Alhosna Benjdia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Thiostrepton Biosynthesis: Prototype for a New Family of Bacteriocins

(2009) Wendy L. Kelly et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In Vitro Characterization of AtsB, a Radical SAM Formylglycine-Generating Enzyme That Contains Three [4Fe-4S] Clusters†

(2008) Tyler L. Grove et al.   BIOCHEMISTRY

The Radical SAM Superfamily

(2008) Perry A. Frey et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

DNA Repair and Free Radicals, New Insights into the Mechanism of Spore Photoproduct Lyase Revealed by Single Amino Acid Substitution

(2008) Alexia Chandor-Proust et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Anaerobic Sulfatase-maturating Enzymes, First Dual Substrate RadicalS-Adenosylmethionine Enzymes

(2008) Alhosna Benjdia et al.   JOURNAL OF BIOLOGICAL CHEMISTRY