Structural insights into the catalysis and regulation of E3 ubiquitin ligases

Functional role of TRIM E3 ligase oligomerization and regulation of catalytic activity

(2016) Marios G Koliopoulos et al.   EMBO JOURNAL

Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation

(2016) Bernhard C. Lechtenberg et al.   NATURE

Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis

(2015) A. Kumar et al.   EMBO JOURNAL

A Ubl/ubiquitin switch in the activation of Parkin

(2015) V. Sauve et al.   EMBO JOURNAL

Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation

(2015) A. Kazlauskaite et al.   EMBO REPORTS

Itch WW Domains Inhibit Its E3 Ubiquitin Ligase Activity by Blocking E2-E3 Ligase Trans-thiolation

(2015) Christopher Riling et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Activation of a Primed RING E3-E2–Ubiquitin Complex by Non-Covalent Ubiquitin

(2015) Lori Buetow et al.   MOLECULAR CELL

Atomic structure of the APC/C and its mechanism of protein ubiquitination

(2015) Leifu Chang et al.   NATURE

Mechanism of phospho-ubiquitin-induced PARKIN activation

(2015) Tobias Wauer et al.   NATURE

Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation

(2015) Daniel Rösner et al.   Nature Protocols

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains

(2015) Emma Branigan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Secondary ubiquitin-RING docking enhances Arkadia and Ark2C E3 ligase activity

(2015) Joshua D Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy

(2015) Alban Ordureau et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex

(2015) Nicholas G. Brown et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains

(2015) Emma Branigan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Secondary ubiquitin-RING docking enhances Arkadia and Ark2C E3 ligase activity

(2015) Joshua D Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RBR E3 ubiquitin ligases: new structures, new insights, new questions

(2014) Donald E. Spratt et al.   BIOCHEMICAL JOURNAL

Parkin is activated by PINK1-dependent phosphorylation of ubiquitin at Ser65

(2014) Agne Kazlauskaite et al.   BIOCHEMICAL JOURNAL

Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8

(2014) Daniel C. Scott et al.   CELL

Insights into the anaphase-promoting complex: a molecular machine that regulates mitosis

(2014) Leifu Chang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RBR E3-ligases at work

(2014) J. J. Smit et al.   EMBO REPORTS

PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity

(2014) Lesley A. Kane et al.   JOURNAL OF CELL BIOLOGY

Ubiquitin Chain Elongation Requires E3-Dependent Tracking of the Emerging Conjugate

(2014) Aileen Kelly et al.   MOLECULAR CELL

Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly

(2014) Nicholas G. Brown et al.   MOLECULAR CELL

Quantitative Proteomics Reveal a Feedforward Mechanism for Mitochondrial PARKIN Translocation and Ubiquitin Chain Synthesis

(2014) Alban Ordureau et al.   MOLECULAR CELL

Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome

(2014) Robert K. McGinty et al.   NATURE

Ubiquitin is phosphorylated by PINK1 to activate parkin

(2014) Fumika Koyano et al.   NATURE

Molecular architecture and mechanism of the anaphase-promoting complex

(2014) Leifu Chang et al.   NATURE

Ubiquitination in disease pathogenesis and treatment

(2014) Doris Popovic et al.   NATURE MEDICINE

Internal motions prime cIAP1 for rapid activation

(2014) Aaron H Phillips et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural and Functional Framework for the Autoinhibition of Nedd4-Family Ubiquitin Ligases

(2014) Sara Mari et al.   STRUCTURE

PINK1-Mediated Phosphorylation of Parkin Boosts Parkin Activity in Drosophila

(2014) Kahori Shiba-Fukushima et al.   PLoS Genetics

Activation of UbcH5c∼Ub Is the Result of a Shift in Interdomain Motions of the Conjugate Bound to U-Box E3 Ligase E4B

(2013) Sarah E. Soss et al.   BIOCHEMISTRY

Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine

(2013) Ranabir Das et al.   EMBO JOURNAL

Structure of the human Parkin ligase domain in an autoinhibited state

(2013) Tobias Wauer et al.   EMBO JOURNAL

Building and remodelling Cullin-RING E3 ubiquitin ligases

(2013) John R Lydeard et al.   EMBO REPORTS

E6AP/UBE3A Ubiquitin Ligase Harbors Two E2∼ubiquitin Binding Sites

(2013) Virginia P. Ronchi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Auto-ubiquitination of Mdm2 Enhances Its Substrate Ubiquitin Ligase Activity

(2013) Ruchira S. Ranaweera et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Structurally Unique E2-Binding Domain Activates Ubiquitination by the ERAD E2, Ubc7p, through Multiple Mechanisms

(2013) Meredith B. Metzger et al.   MOLECULAR CELL

Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization

(2013) Shireen A. Sarraf et al.   NATURE

Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP

(2013) Benjamin Stieglitz et al.   NATURE

Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex

(2013) Weiping Wang et al.   NATURE CELL BIOLOGY

A conserved asparagine has a structural role in ubiquitin-conjugating enzymes

(2013) Christopher E Berndsen et al.   Nature Chemical Biology

Electron microscopy structure of human APC/CCDH1–EMI1 reveals multimodal mechanism of E3 ligase shutdown

(2013) Jeremiah J Frye et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming

(2013) Elena Maspero et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Essentiality of a non-RING element in priming donor ubiquitin for catalysis by a monomeric E3

(2013) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the HHARI Catalytic Domain Shows Glimpses of a HECT E3 Ligase

(2013) Donald E. Spratt et al.   PLoS One

Structure of Parkin Reveals Mechanisms for Ubiquitin Ligase Activation

(2013) J.-F. Trempe et al.   SCIENCE

Structure of HHARI, a RING-IBR-RING Ubiquitin Ligase: Autoinhibition of an Ariadne-Family E3 and Insights into Ligation Mechanism

(2013) David M. Duda et al.   STRUCTURE

A molecular explanation for the recessive nature of parkin-linked Parkinson’s disease

(2013) Donald E. Spratt et al.   Nature Communications

Structure and function of Parkin E3 ubiquitin ligase reveals aspects of RING and HECT ligases

(2013) B.E. Riley et al.   Nature Communications

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

(2013) Hari B Kamadurai et al.   eLife

Ubiquitin-Binding Proteins: Decoders of Ubiquitin-Mediated Cellular Functions

(2012) Koraljka Husnjak et al.   Annual Review of Biochemistry

The Ubiquitin Code

(2012) David Komander et al.   Annual Review of Biochemistry

Structural Insights into the Conformation and Oligomerization of E2∼Ubiquitin Conjugates

(2012) Richard C. Page et al.   BIOCHEMISTRY

The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension

(2012) Judith J Smit et al.   EMBO JOURNAL

LUBAC synthesizes linear ubiquitin chains via a thioester intermediate

(2012) Benjamin Stieglitz et al.   EMBO REPORTS

The role of ubiquitylation in receptor endocytosis and endosomal sorting

(2012) K. Haglund et al.   JOURNAL OF CELL SCIENCE

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

Targeting IAP proteins for therapeutic intervention in cancer

(2012) Simone Fulda et al.   NATURE REVIEWS DRUG DISCOVERY

Atypical ubiquitylation — the unexplored world of polyubiquitin beyond Lys48 and Lys63 linkages

(2012) Yogesh Kulathu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

BIRC7–E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural Basis for the Versatile Interactions of Smad7 with Regulator WW Domains in TGF-β Pathways

(2012) Eric Aragón et al.   STRUCTURE

PINK1-mediated phosphorylation of the Parkin ubiquitin-like domain primes mitochondrial translocation of Parkin and regulates mitophagy

(2012) Kahori Shiba-Fukushima et al.   Scientific Reports

PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65

(2012) C. Kondapalli et al.   Open Biology

Expanded Click Conjugation of Recombinant Proteins with Ubiquitin-Like Modifiers Reveals Altered Substrate Preference of SUMO2-Modified Ubc9

(2011) Stefanie Sommer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate

(2011) Jonathan N. Pruneda et al.   BIOCHEMISTRY

The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2

(2011) Katherine E. Wickliffe et al.   CELL

Generation of a Mono-ubiquitinated PCNA Mimic by Click Chemistry

(2011) Silvia Eger et al.   CHEMBIOCHEM

Autoregulation of Parkin activity through its ubiquitin-like domain

(2011) Viduth K Chaugule et al.   EMBO JOURNAL

Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation

(2011) Elena Maspero et al.   EMBO REPORTS

Structure and function of a HECT domain ubiquitin-binding site

(2011) Hyung Cheol Kim et al.   EMBO REPORTS

Smac Mimetics Activate the E3 Ligase Activity of cIAP1 Protein by Promoting RING Domain Dimerization

(2011) Rebecca Feltham et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CARD-Mediated Autoinhibition of cIAP1's E3 Ligase Activity Suppresses Cell Proliferation and Migration

(2011) Juanita Lopez et al.   MOLECULAR CELL

Essential Role for Ubiquitin-Ubiquitin-Conjugating Enzyme Interaction in Ubiquitin Discharge from Cdc34 to Substrate

(2011) Anjanabha Saha et al.   MOLECULAR CELL

Cdh1 Regulates Osteoblast Function through an APC/C-Independent Modulation of Smurf1

(2011) Lixin Wan et al.   MOLECULAR CELL

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids

(2011) Dawn M. Wenzel et al.   NATURE

A RING E3–substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases

(2011) Matthew F Calabrese et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanism of ubiquitylation by dimeric RING ligase RNF4

(2011) Anna Plechanovová et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6

(2011) R. G. Hibbert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Autoinhibition and phosphorylation-induced activation mechanisms of human cancer and autoimmune disease-related E3 protein Cbl-b

(2011) Y. Kobashigawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antagonists Induce a Conformational Change in cIAP1 That Promotes Autoubiquitination

(2011) E. C. Dueber et al.   SCIENCE

Calcium Activates Nedd4 E3 Ubiquitin Ligases by Releasing the C2 Domain-mediated Auto-inhibition

(2010) Jian Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis

(2010) Junjun Chen et al.   Nature Chemical Biology

Ubiquitin signalling in DNA replication and repair

(2010) Helle D. Ulrich et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Molecular Basis for the Association of Human E4B U Box Ubiquitin Ligase with E2-Conjugating Enzymes UbcH5c and Ubc4

(2010) Robert C. Benirschke et al.   STRUCTURE

Crystal Structure of UbcH5b∼Ubiquitin Intermediate: Insight into the Formation of the Self-Assembled E2∼Ub Conjugates

(2010) Eri Sakata et al.   STRUCTURE

RING Domain E3 Ubiquitin Ligases

(2009) Raymond J. Deshaies et al.   Annual Review of Biochemistry

Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome

(2009) Daniel Finley   Annual Review of Biochemistry

Control of the activity of WW-HECT domain E3 ubiquitin ligases by NDFIP proteins

(2009) Thomas Mund et al.   EMBO REPORTS

Regulation of the RSP5 Ubiquitin Ligase by an Intrinsic Ubiquitin-binding Site

(2009) Michael E. French et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nonproteolytic Functions of Ubiquitin in Cell Signaling

(2009) Zhijian J. Chen et al.   MOLECULAR CELL

Insights into Ubiquitin Transfer Cascades from a Structure of a UbcH5B∼Ubiquitin-HECTNEDD4L Complex

(2009) Hari B. Kamadurai et al.   MOLECULAR CELL

Allosteric Activation of E2-RING Finger-Mediated Ubiquitylation by a Structurally Defined Specific E2-Binding Region of gp78

(2009) Ranabir Das et al.   MOLECULAR CELL

Building ubiquitin chains: E2 enzymes at work

(2009) Yihong Ye et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Physiological functions of the HECT family of ubiquitin ligases

(2009) Daniela Rotin et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

E2 interaction and dimerization in the crystal structure of TRAF6

(2009) Qian Yin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Large-scale detection of ubiquitination substrates using cell extracts and protein microarrays

(2009) Yifat Merbl et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis of UV DNA-Damage Recognition by the DDB1–DDB2 Complex

(2008) Andrea Scrima et al.   CELL

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation

(2008) David M. Duda et al.   CELL

Mechanism of Ubiquitin-Chain Formation by the Human Anaphase-Promoting Complex

(2008) Lingyan Jin et al.   CELL

Structures of the cIAP2 RING Domain Reveal Conformational Changes Associated with Ubiquitin-conjugating Enzyme (E2) Recruitment

(2008) Peter D. Mace et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Parkin is recruited selectively to impaired mitochondria and promotes their autophagy

(2008) Derek Narendra et al.   JOURNAL OF CELL BIOLOGY

Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation

(2008) Robert K. McGinty et al.   NATURE

Genome-Wide and Functional Annotation of Human E3 Ubiquitin Ligases Identifies MULAN, a Mitochondrial E3 that Regulates the Organelle's Dynamics and Signaling

(2008) Wei Li et al.   PLoS One