A conserved asparagine has a structural role in ubiquitin-conjugating enzymes

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

BIRC7–E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

REFMAC5 for the refinement of macromolecular crystal structures

(2011) Garib N. Murshudov et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins

(2011) Christopher E. Berndsen et al.   ANALYTICAL BIOCHEMISTRY

Essential Role for Ubiquitin-Ubiquitin-Conjugating Enzyme Interaction in Ubiquitin Discharge from Cdc34 to Substrate

(2011) Anjanabha Saha et al.   MOLECULAR CELL

A New Generation of Crystallographic Validation Tools for the Protein Data Bank

(2011) Randy J. Read et al.   STRUCTURE

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

E2s: structurally economical and functionally replete

(2010) Dawn M. Wenzel et al.   BIOCHEMICAL JOURNAL

Crystal Structure of UbcH5b∼Ubiquitin Intermediate: Insight into the Formation of the Self-Assembled E2∼Ub Conjugates

(2010) Eri Sakata et al.   STRUCTURE

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

(2009) Huanting Liu et al.   BMC BIOTECHNOLOGY

Synthesis of Free and Proliferating Cell Nuclear Antigen-bound Polyubiquitin Chains by the RING E3 Ubiquitin Ligase Rad5

(2009) Candice M. Carlile et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Insights into Ubiquitin Transfer Cascades from a Structure of a UbcH5B∼Ubiquitin-HECTNEDD4L Complex

(2009) Hari B. Kamadurai et al.   MOLECULAR CELL