Multi-scale cellular imaging of DNA double strand break repair

Live-cell tracking of γ-H2AX kinetics reveals the distinct modes of ATM and DNA-PK in the immediate response to DNA damage

(2023) Watanya Trakarnphornsombat et al.   JOURNAL OF CELL SCIENCE

Systematic analysis of the molecular and biophysical properties of key DNA damage response factors

(2023) Joshua R Heyza et al.   eLife

Live cell single-molecule imaging to study DNA repair in human cells

(2023) Joshua R. Heyza et al.   DNA REPAIR

Super-Resolution Microscopy for Structural Cell Biology

(2022) Sheng Liu et al.   Annual Review of Biophysics

Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging

(2022) Jyothi Mahadevan et al.   iScience

Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination

(2021) Donna R. Whelan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single molecule microscopy reveals key physical features of repair foci in living cells

(2021) Judith Miné-Hattab et al.   eLife

Fully automated counting of DNA damage foci in tumor cell culture: A matter of cell separation

(2021) S. Köcher et al.   DNA REPAIR

A simple microscopy setup for visualizing cellular responses to DNA damage at particle accelerator facilities

(2021) Haibin Qian et al.   Scientific Reports

Role of BRCA2 DNA-binding and C-terminal domain in its mobility and conformation in DNA repair

(2021) Maarten W Paul et al.   eLife

A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress

(2021) Eric Moeglin et al.   Cancers

Assessing kinetics and recruitment of DNA repair factors using high content screens

(2021) Barbara Martinez-Pastor et al.   Cell Reports

Physical observables to determine the nature of membrane-less cellular sub-compartments

(2021) Mathias L Heltberg et al.   eLife

DNA Double Strand Break Repair Pathways in Response to Different Types of Ionizing Radiation

(2021) Gerarda van de Kamp et al.   Frontiers in Genetics

FocAn: automated 3D analysis of DNA repair foci in image stacks acquired by confocal fluorescence microscopy

(2020) Simon Memmel et al.   BMC BIOINFORMATICS

Evaluation of machine learning models for automatic detection of DNA double strand breaks after irradiation using a γH2AX foci assay

(2020) Tim Hohmann et al.   PLoS One

DNA repair by Rad52 liquid droplets

(2020) Roxanne Oshidari et al.   Nature Communications

Inferring quantity and qualities of superimposed reaction rates from single molecule survival time distributions

(2020) Matthias Reisser et al.   Scientific Reports

Genome Editing Fidelity in the Context of DNA Sequence and Chromatin Structure

(2020) Lyuba Chechik et al.   Frontiers in Cell and Developmental Biology

Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination

(2020) J. Brooks Crickard et al.   CELL

Very fast CRISPR on demand

(2020) Yang Liu et al.   SCIENCE

Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophase

(2020) Johan A. Slotman et al.   PLoS Genetics

Deep-Learning-Assisted Assessment of DNA Damage Based on Foci Images and Its Application in High-Content Screening of Lead Compounds

(2020) Xuechun Chen et al.   ANALYTICAL CHEMISTRY

Comparison of High- and Low-LET Radiation-Induced DNA Double-Strand Break Processing in Living Cells

(2020) Stefan J. Roobol et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The molecular basis and disease relevance of non-homologous DNA end joining

(2020) Bailin Zhao et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Live cell monitoring of double strand breaks in S. cerevisiae

(2019) David P. Waterman et al.   PLoS Genetics

Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0

(2019) Venkatakaushik Voleti et al.   NATURE METHODS

Endogenous Fluorescence Tagging by CRISPR

(2019) Hassan Bukhari et al.   TRENDS IN CELL BIOLOGY

Stabilization of chromatin topology safeguards genome integrity

(2019) Fena Ochs et al.   NATURE

Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells

(2019) Mehdi Banan   JOURNAL OF BIOTECHNOLOGY

DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity

(2018) Rajat Gupta et al.   CELL

Live Dynamics of 53BP1 Foci Following Simultaneous Induction of Clustered and Dispersed DNA Damage in U2OS Cells

(2018) Alice Sollazzo et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

(2018) Sascha Conic et al.   JOURNAL OF CELL BIOLOGY

Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks

(2018) Eva K. Brinkman et al.   MOLECULAR CELL

Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing

(2018) Birgit Koch et al.   Nature Protocols

Single-molecule localization microscopy reveals molecular transactions during RAD51 filament assembly at cellular DNA damage sites

(2018) Kalina T Haas et al.   NUCLEIC ACIDS RESEARCH

Robust model-based analysis of single-particle tracking experiments with Spot-On

(2018) Anders S Hansen et al.   eLife

Preserving replication fork integrity and competence via the homologous recombination pathway

(2018) Anissia Ait Saada et al.   DNA REPAIR

Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks

(2018) Donna R. Whelan et al.   Nature Communications

Content-aware image restoration: pushing the limits of fluorescence microscopy

(2018) Martin Weigert et al.   NATURE METHODS

AutoFoci, an automated high-throughput foci detection approach for analyzing low-dose DNA double-strand break repair

(2018) Nicor Lengert et al.   Scientific Reports

Genome Instability and γH2AX

(2017) Anastasios Georgoulis et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

(2017) François Aymard et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Meta-analysis of DNA double-strand break response kinetics

(2017) Jakub A. Kochan et al.   NUCLEIC ACIDS RESEARCH

Architectural plasticity of human BRCA2–RAD51 complexes in DNA break repair

(2017) Humberto Sánchez et al.   NUCLEIC ACIDS RESEARCH

Identification of the elementary structural units of the DNA damage response

(2017) Francesco Natale et al.   Nature Communications

Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

(2017) François Aymard et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CTCF and cohesin regulate chromatin loop stability with distinct dynamics

(2017) Anders S Hansen et al.   eLife

Interplay between Fanconi anemia and homologous recombination pathways in genome integrity

(2016) Johanna Michl et al.   EMBO JOURNAL

Temporal and Spatial Uncoupling of DNA Double Strand Break Repair Pathways within Mammalian Heterochromatin

(2016) Katerina Tsouroula et al.   MOLECULAR CELL

Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes

(2016) Francisco Balzarotti et al.   SCIENCE

Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

(2016) Nils Gustafsson et al.   Nature Communications

FoCo: a simple and robust quantification algorithm of nuclear foci

(2015) Anastasiya Lapytsko et al.   BMC BIOINFORMATICS

53BP1 and the LINC Complex Promote Microtubule-Dependent DSB Mobility and DNA Repair

(2015) Francisca Lottersberger et al.   CELL

A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity

(2015) Evert-Jan Uringa et al.   DNA REPAIR

DNA damage foci: Meaning and significance

(2015) Kai Rothkamm et al.   ENVIRONMENTAL AND MOLECULAR MUTAGENESIS

Imaging Live-Cell Dynamics and Structure at the Single-Molecule Level

(2015) Zhe Liu et al.   MOLECULAR CELL

Inducible in vivo genome editing with CRISPR-Cas9

(2015) Lukas E Dow et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

A general method to improve fluorophores for live-cell and single-molecule microscopy

(2015) Jonathan B Grimm et al.   NATURE METHODS

Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution

(2015) Katharina Schücker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Small Rad51 and Dmc1 Complexes Often Co-occupy Both Ends of a Meiotic DNA Double Strand Break

(2015) M. Scott Brown et al.   PLoS Genetics

Generation of an alpaca-derived nanobody recognizing γ-H2AX

(2015) Malini Rajan et al.   FEBS Open Bio

Green fluorescent protein fused to the C terminus of RAD51 specifically interferes with secondary DNA binding by the RAD51-ssDNA complex

(2015) Wataru Kobayashi et al.   GENES & GENETIC SYSTEMS

Differential binding kinetics of replication protein A during replication and the pre- and post-incision steps of nucleotide excision repair

(2014) Audrey M. Gourdin et al.   DNA REPAIR

BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells

(2014) Marcel Reuter et al.   JOURNAL OF CELL BIOLOGY

Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

(2014) Robert Mahen et al.   MOLECULAR BIOLOGY OF THE CELL

FindFoci: A Focus Detection Algorithm with Automated Parameter Training That Closely Matches Human Assignments, Reduces Human Inconsistencies and Increases Speed of Analysis

(2014) Alex D. Herbert et al.   PLoS One

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

(2014) Bi-Chang Chen et al.   SCIENCE

Mediators of Homologous DNA Pairing

(2014) A. Zelensky et al.   Cold Spring Harbor Perspectives in Biology

Single-molecule tracking in live cells reveals distinct target-search strategies of transcription factors in the nucleus

(2014) Ignacio Izeddin et al.   eLife

3D imaging of Sox2 enhancer clusters in embryonic stem cells

(2014) Zhe Liu et al.   eLife

A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair

(2013) Sébastien Britton et al.   JOURNAL OF CELL BIOLOGY

Monitoring Homology Search during DNA Double-Strand Break Repair In Vivo

(2013) Jörg Renkawitz et al.   MOLECULAR CELL

RPA Coordinates DNA End Resection and Prevents Formation of DNA Hairpins

(2013) Huan Chen et al.   MOLECULAR CELL

Double strand break repair functions of histone H2AX

(2013) Ralph Scully et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

The transience of transient overexpression

(2013) Toby J Gibson et al.   NATURE METHODS

Double-strand break repair: 53BP1 comes into focus

(2013) Stephanie Panier et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Role of 53BP1 oligomerization in regulating double-strand break repair

(2013) F. Lottersberger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

DNA interstrand crosslinks induce a potent replication block followed by formation and repair of double strand breaks in intact mammalian cells

(2012) Daniel Vare et al.   DNA REPAIR

The in vivo dynamic interplay of MDC1 and 53BP1 at DNA damage-induced nuclear foci

(2012) Myth T.S. Mok et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair

(2012) J. R. Chapman et al.   JOURNAL OF CELL SCIENCE

Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution

(2012) A. Loschberger et al.   JOURNAL OF CELL SCIENCE

Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

(2012) Sara Abrahamsson et al.   NATURE METHODS

The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis

(2012) M.-T. Kurzbauer et al.   PLANT CELL

Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications

(2011) S. E. Polo et al.   GENES & DEVELOPMENT

ATP-dependent and independent functions of Rad54 in genome maintenance

(2011) Sheba Agarwal et al.   JOURNAL OF CELL BIOLOGY

Automated analysis of time-lapse fluorescence microscopy images: from live cell images to intracellular foci

(2010) O. Dzyubachyk et al.   BIOINFORMATICS

ATM-Dependent Chromatin Changes Silence Transcription In cis to DNA Double-Strand Breaks

(2010) Niraj M. Shanbhag et al.   CELL

Deciphering the chromatin landscape induced around DNA double strand breaks

(2010) Laurent Massip et al.   CELL CYCLE

Alkylation DNA damage in combination with PARP inhibition results in formation of S-phase-dependent double-strand breaks

(2010) Michelle L. Heacock et al.   DNA REPAIR

Assembly and function of DNA double-strand break repair foci in mammalian cells

(2010) Simon Bekker-Jensen et al.   DNA REPAIR

High-resolution profiling of γH2AX around DNA double strand breaks in the mammalian genome

(2010) Jason S Iacovoni et al.   EMBO JOURNAL

Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA

(2010) Jie Liu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

(2009) Carel van Oven et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation

(2009) Sophie Badie et al.   JOURNAL OF CELL BIOLOGY

Specific local induction of DNA strand breaks by infrared multi-photon absorption

(2009) D. Träutlein et al.   NUCLEIC ACIDS RESEARCH

Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells

(2009) Xiangduo Kong et al.   NUCLEIC ACIDS RESEARCH

Crosstalk between histone modifications during the DNA damage response

(2009) Haico van Attikum et al.   TRENDS IN CELL BIOLOGY

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility

(2008) Nadya Dimitrova et al.   NATURE

Induction of linear tracks of DNA double-strand breaks by α-particle irradiation of cells

(2008) Jan Stap et al.   NATURE METHODS

Highly inclined thin illumination enables clear single-molecule imaging in cells

(2008) Makio Tokunaga et al.   NATURE METHODS