Assessing kinetics and recruitment of DNA repair factors using high content screens

A Snapshot on the Cis Chromatin Response to DNA Double-Strand Breaks

(2019) Thomas Clouaire et al.   TRENDS IN GENETICS

DNA double-strand break repair-pathway choice in somatic mammalian cells

(2019) Ralph Scully et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage

(2019) Rebecca Smith et al.   NUCLEIC ACIDS RESEARCH

Protein Dynamics in Complex DNA Lesions

(2018) Radoslav Aleksandrov et al.   MOLECULAR CELL

PARP-1–dependent recruitment of cold-inducible RNA-binding protein promotes double-strand break repair and genome stability

(2018) Jung-Kuei Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CellProfiler 3.0: Next-generation image processing for biology

(2018) Claire McQuin et al.   PLOS BIOLOGY

53BP1 cooperation with the REV7–shieldin complex underpins DNA structure-specific NHEJ

(2018) Hind Ghezraoui et al.   NATURE

The shieldin complex mediates 53BP1-dependent DNA repair

(2018) Sylvie M. Noordermeer et al.   NATURE

53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in

(2018) Zachary Mirman et al.   NATURE

The poly(ADP-ribose)-dependent chromatin remodeler Alc1 induces local chromatin relaxation upon DNA damage

(2016) Hafida Sellou et al.   MOLECULAR BIOLOGY OF THE CELL

PARP1 Links CHD2-Mediated Chromatin Expansion and H3.3 Deposition to DNA Repair by Non-homologous End-Joining

(2016) Martijn S. Luijsterburg et al.   MOLECULAR CELL

The TIP60 Complex Regulates Bivalent Chromatin Recognition by 53BP1 through Direct H4K20me Binding and H2AK15 Acetylation

(2016) Karine Jacquet et al.   MOLECULAR CELL

Cells and Stripes: A novel quantitative photo-manipulation technique

(2016) Martin Mistrik et al.   Scientific Reports

Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination

(2015) Fade Gong et al.   GENES & DEVELOPMENT

Poly(ADP-ribosyl)ation-dependent Transient Chromatin Decondensation and Histone Displacement following Laser Microirradiation

(2015) Hilmar Strickfaden et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors

(2015) Lior Izhar et al.   Cell Reports

DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair

(2014) Ismail Abdou et al.   NUCLEIC ACIDS RESEARCH

Activation of DNA Damage Response Signaling by Condensed Chromatin

(2014) Rebecca C. Burgess et al.   Cell Reports

To spread or not to spread—chromatin modifications in response to DNA damage

(2013) Matthias Altmeyer et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

A Cell Cycle-Dependent Regulatory Circuit Composed of 53BP1-RIF1 and BRCA1-CtIP Controls DNA Repair Pathway Choice

(2013) Cristina Escribano-Díaz et al.   MOLECULAR CELL

Proteome-wide Identification of Poly(ADP-Ribosyl)ation Targets in Different Genotoxic Stress Responses

(2013) Stephanie Jungmichel et al.   MOLECULAR CELL

SIRT6 Recruits SNF2H to DNA Break Sites, Preventing Genomic Instability through Chromatin Remodeling

(2013) Debra Toiber et al.   MOLECULAR CELL

53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark

(2013) Amélie Fradet-Turcotte et al.   NATURE

The bromodomain protein Brd4 insulates chromatin from DNA damage signalling

(2013) Scott R. Floyd et al.   NATURE

Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination

(2013) Jiangbo Tang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Reading, writing, and repair: the role of ubiquitin and the ubiquitin-like proteins in DNA damage signaling and repair

(2013) Jordan B. Pinder et al.   Frontiers in Genetics

RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites

(2012) Frédérick A Mallette et al.   EMBO JOURNAL

Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response

(2012) Petra Beli et al.   MOLECULAR CELL

Histone H2A.Z Controls a Critical Chromatin Remodeling Step Required for DNA Double-Strand Break Repair

(2012) Ye Xu et al.   MOLECULAR CELL

A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response

(2012) Britt Adamson et al.   NATURE CELL BIOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

PHF20 is an effector protein of p53 double lysine methylation that stabilizes and activates p53

(2012) Gaofeng Cui et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Loss of the Methyl Lysine Effector Protein PHF20 Impacts the Expression of Genes Regulated by the Lysine Acetyltransferase MOF

(2011) Aimee I. Badeaux et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A public genome-scale lentiviral expression library of human ORFs

(2011) Xiaoping Yang et al.   NATURE METHODS

The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks

(2011) Klara Acs et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A cell-based screen identifies ATR inhibitors with synthetic lethal properties for cancer-associated mutations

(2011) Luis I Toledo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

53BP1 Inhibits Homologous Recombination in Brca1-Deficient Cells by Blocking Resection of DNA Breaks

(2010) Samuel F. Bunting et al.   CELL

High resolution imaging of changes in the structure and spatial organization of chromatin, γ-H2A.X and the MRN complex within etoposide-induced DNA repair foci

(2010) Graham Dellaire et al.   CELL CYCLE

Assembly and function of DNA double-strand break repair foci in mammalian cells

(2010) Simon Bekker-Jensen et al.   DNA REPAIR

53BP1 regulates DNA resection and the choice between classical and alternative end joining during class switch recombination

(2010) Anne Bothmer et al.   JOURNAL OF EXPERIMENTAL MEDICINE

The DNA Damage Response: Making It Safe to Play with Knives

(2010) Alberto Ciccia et al.   MOLECULAR CELL

A chromatin localization screen reveals poly (ADP ribose)-regulated recruitment of the repressive polycomb and NuRD complexes to sites of DNA damage

(2010) D. M. Chou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNF168 Binds and Amplifies Ubiquitin Conjugates on Damaged Chromosomes to Allow Accumulation of Repair Proteins

(2009) Carsten Doil et al.   CELL

The RIDDLE Syndrome Protein Mediates a Ubiquitin-Dependent Signaling Cascade at Sites of DNA Damage

(2009) Grant S. Stewart et al.   CELL

MRG15 Is a Novel PALB2-interacting Factor Involved in Homologous Recombination

(2009) Shirley M.-H. Sy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Subunit Composition and Substrate Specificity of a MOF-containing Histone Acetyltransferase Distinct from the Male-specific Lethal (MSL) Complex

(2009) Yong Cai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identification of genes associated with non-small-cell lung cancer promotion and progression

(2009) Jasna Bankovic et al.   LUNG CANCER

CtIP-BRCA1 modulates the choice of DNA double-strand-break repair pathway throughout the cell cycle

(2009) Maximina H. Yun et al.   NATURE

The DNA-damage response in human biology and disease

(2009) Stephen P. Jackson et al.   NATURE

Alternative-NHEJ Is a Mechanistically Distinct Pathway of Mammalian Chromosome Break Repair

(2008) Nicole Bennardo et al.   PLoS Genetics