Illuminating GPCR signaling mechanisms by NMR spectroscopy with stable-isotope labeled receptors

Filling of a water-free void explains the allosteric regulation of the β1-adrenergic receptor by cholesterol

(2022) Layara Akemi Abiko et al.   Nature Chemistry

Conformational selection guides β-arrestin recruitment at a biased G protein–coupled receptor

(2022) Andrew B. Kleist et al.   SCIENCE

Advances in the study of GPCRs by 19F NMR

(2021) Louis-Philippe Picard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Molecular insights into the biased signaling mechanism of the μ-opioid receptor

(2021) Xiaojing Cong et al.   MOLECULAR CELL

Chemical Synthesis of a Full-Length G-Protein-Coupled Receptor β2-Adrenergic Receptor with Defined Modification Patterns at the C-Terminus

(2021) Yulei Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural equilibrium underlying ligand-dependent activation of β2-adrenoreceptor

(2020) Shunsuke Imai et al.   Nature Chemical Biology

Probing the correlation between ligand efficacy and conformational diversity at the α1A-adrenoreceptor reveals allosteric coupling of its microswitches

(2020) Feng-Jie Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Conserved Proline Hinge Mediates Helix Dynamics and Activation of Rhodopsin

(2020) Andreyah L. Pope et al.   STRUCTURE

A high-resolution description of β1-adrenergic receptor functional dynamics and allosteric coupling from backbone NMR

(2020) Anne Grahl et al.   Nature Communications

Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR

(2020) Takuya Mizumura et al.   Science Advances

Beyond structure: emerging approaches to study GPCR dynamics

(2020) Anastasiia Gusach et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Probing the Conformational States of Neurotensin Receptor 1 Variants by NMR Site‐Directed Methyl Labeling

(2020) Inguna Goba et al.   CHEMBIOCHEM

Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches

(2020) Andrew J. Y. Jones et al.   MOLECULES

Analysis of β2AR-Gs and β2AR-Gi complex formation by NMR spectroscopy

(2020) Xiuyan Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy

(2020) Philip Rößler et al.   MOLECULES

A2A Adenosine Receptor Partial Agonism Related to Structural Rearrangements in an Activation Microswitch

(2020) Matthew T. Eddy et al.   STRUCTURE

Conformational Complexity and Dynamics in a Muscarinic Receptor Revealed by NMR Spectroscopy

(2019) Jun Xu et al.   MOLECULAR CELL

Optimization and 13 CH 3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies

(2018) Fabian Bumbak et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A 2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   CELL

Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

(2018) Yutaka Kofuku et al.   JOURNAL OF BIOMOLECULAR NMR

Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR

(2018) Yutaro Shiraishi et al.   Nature Communications

On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies

(2018) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures

(2018) Ichio Shimada et al.   NATURE REVIEWS DRUG DISCOVERY

Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor

(2017) Andras S. Solt et al.   Nature Communications

Ligand modulation of sidechain dynamics in a wild-type human GPCR

(2017) Lindsay D Clark et al.   eLife

GPCR Dynamics: Structures in Motion

(2016) Naomi R. Latorraca et al.   CHEMICAL REVIEWS

Functional Modulation of a G Protein-Coupled Receptor Conformational Landscape in a Lipid Bilayer

(2016) Marina Casiraghi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

(2016) Giovanni Luchetti et al.   eLife

Identification of a Conformational Equilibrium That Determines the Efficacy and Functional Selectivity of the μ-Opioid Receptor

(2015) Junya Okude et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and1H Detection

(2015) Yongchao Su et al.   Annual Review of Biochemistry

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts

(2015) Christian Opitz et al.   JOURNAL OF BIOMOLECULAR NMR

Propagation of conformational changes during μ-opioid receptor activation

(2015) Rémy Sounier et al.   NATURE

Characterization of the Simultaneous Decay Kinetics of Metarhodopsin States II and III in Rhodopsin by Solution-State NMR Spectroscopy

(2014) Jochen Stehle et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Two Classes of Cholesterol Binding Sites for the β 2 AR Revealed by Thermostability and NMR

(2014) Deborah L. Gater et al.   BIOPHYSICAL JOURNAL

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs

(2013) Tatiana Didenko et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin

(2013) T. Zhuang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure-Function of the G Protein–Coupled Receptor Superfamily

(2012) Vsevolod Katritch et al.   Annual Review of Pharmacology and Toxicology

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

(2010) Michael P. Bokoch et al.   NATURE

Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation

(2009) Shivani Ahuja et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR

(2008) A. B. Barnes et al.   APPLIED MAGNETIC RESONANCE