On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A 2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   CELL

Production of isotope-labeled proteins in insect cells for NMR

(2018) Bastian Franke et al.   JOURNAL OF BIOMOLECULAR NMR

Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system

(2018) Rika Suzuki et al.   JOURNAL OF BIOMOLECULAR NMR

Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

(2018) Yutaka Kofuku et al.   JOURNAL OF BIOMOLECULAR NMR

An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins

(2017) Chih-Ta Henry Chien et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

GPCRdb in 2018: adding GPCR structure models and ligands

(2017) Gáspár Pándy-Szekeres et al.   NUCLEIC ACIDS RESEARCH

Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor

(2017) Andras S. Solt et al.   Nature Communications

Ligand modulation of sidechain dynamics in a wild-type human GPCR

(2017) Lindsay D Clark et al.   eLife

Sparse 13C labelling for solid-state NMR studies of P. pastoris expressed eukaryotic seven-transmembrane proteins

(2016) Jing Liu et al.   JOURNAL OF BIOMOLECULAR NMR

Functional Modulation of a G Protein-Coupled Receptor Conformational Landscape in a Lipid Bilayer

(2016) Marina Casiraghi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A mechanism of viral immune evasion revealed by cryo-EM analysis of the TAP transporter

(2016) Michael L. Oldham et al.   NATURE

Crystal structure of the human sterol transporter ABCG5/ABCG8

(2016) Jyh-Yeuan Lee et al.   NATURE

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

High-resolution crystal structure of the human CB1 cannabinoid receptor

(2016) Zhenhua Shao et al.   NATURE

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation

(2016) Dean P. Staus et al.   NATURE

Conductance of P2X4purinergic receptor is determined by conformational equilibrium in the transmembrane region

(2016) Yuichi Minato et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

β 2 -Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop

(2016) Matthew T. Eddy et al.   STRUCTURE

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

Pichia pastoris as an expression host for membrane protein structural biology

(2015) Bernadette Byrne   CURRENT OPINION IN STRUCTURAL BIOLOGY

An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts

(2015) Christian Opitz et al.   JOURNAL OF BIOMOLECULAR NMR

Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

(2015) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

Structure of the voltage-gated two-pore channel TPC1 from Arabidopsis thaliana

(2015) Jiangtao Guo et al.   NATURE

Propagation of conformational changes during μ-opioid receptor activation

(2015) Rémy Sounier et al.   NATURE

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Crystal structure of the human OX2 orexin receptor bound to the insomnia drug suvorexant

(2014) Jie Yin et al.   NATURE

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Perdeuteration and methyl-selective 1H, 13C-labeling by using a Kluyveromyces lactis expression system

(2013) Mayumi Miyazawa-Onami et al.   JOURNAL OF BIOMOLECULAR NMR

Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra

(2013) Sanaz Emami et al.   JOURNAL OF BIOMOLECULAR NMR

Can too many copies spoil the broth?

(2013) Rochelle Aw et al.   Microbial Cell Factories

Structure of the chemokine receptor CXCR1 in phospholipid bilayers

(2012) Sang Ho Park et al.   NATURE

G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody

(2012) Tomoya Hino et al.   NATURE

Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans

(2012) Mi Sun Jin et al.   NATURE

Crystal Structure of the Calcium Release-Activated Calcium Channel Orai

(2012) X. Hou et al.   SCIENCE

Biased Signaling Pathways in  2-Adrenergic Receptor Characterized by 19F-NMR

(2012) J. J. Liu et al.   SCIENCE

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

Crystal Structure of the Mammalian GIRK2 K+ Channel and Gating Regulation by G Proteins, PIP2, and Sodium

(2011) Matthew R. Whorton et al.   CELL

Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment

(2011) Ying Fan et al.   JOURNAL OF BIOMOLECULAR NMR

Overexpression of a homogeneous oligosaccharide with 13C labeling by genetically engineered yeast strain

(2011) Yukiko Kamiya et al.   JOURNAL OF BIOMOLECULAR NMR

A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility

(2011) Alvar D. Gossert et al.   JOURNAL OF BIOMOLECULAR NMR

Structure of the human histamine H1 receptor complex with doxepin

(2011) Tatsuro Shimamura et al.   NATURE

Uniform stable-isotope labeling in mammalian cells: formulation of a cost-effective culture medium

(2010) Tatiana A. Egorova-Zachernyuk et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

(2010) Michael P. Bokoch et al.   NATURE

Highly conserved tyrosine stabilizes the active state of rhodopsin

(2010) J. A. Goncalves et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR

(2010) T. L. Religa et al.   SCIENCE

Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

(2009) Takami Yurugi-Kobayashi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Mapping the human membrane proteome: a majority of the human membrane proteins can be classified according to function and evolutionary origin

(2009) Markus Almén et al.   BMC BIOLOGY

Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation

(2009) Shivani Ahuja et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Recent advances in the application of solution NMR spectroscopy to multi-span integral membrane proteins

(2009) Hak Jun Kim et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Solution Structure of the Integral Human Membrane Protein VDAC-1 in Detergent Micelles

(2008) S. Hiller et al.   SCIENCE