GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy

Dynamic G Protein-Coupled Receptor Signaling Probed by Solution NMR Spectroscopy

(2020) Sho Hee Park et al.   BIOCHEMISTRY

Structural equilibrium underlying ligand-dependent activation of β2-adrenoreceptor

(2020) Shunsuke Imai et al.   Nature Chemical Biology

Conformational plasticity of ligand-bound and ternary GPCR complexes studied by 19F NMR of the β1-adrenergic receptor

(2020) J. Niclas Frei et al.   Nature Communications

Impact of GPCR Structures on Drug Discovery

(2020) Miles Congreve et al.   CELL

A high-resolution description of β1-adrenergic receptor functional dynamics and allosteric coupling from backbone NMR

(2020) Anne Grahl et al.   Nature Communications

Dynamics of Bacteriorhodopsin in the Dark‐Adapted State from Solution NMR

(2020) Laurens Kooijman et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enabling NMR studies of high molecular weight systems without the need for deuteration: The XL‐ALSOFAST experiment with delayed decoupling

(2020) Philip Rößler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Conformational Equilibrium of the Neuropeptide Y2 Receptor in Bilayer Membranes

(2020) Ulrike Krug et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Analysis of β2AR-Gs and β2AR-Gi complex formation by NMR spectroscopy

(2020) Xiuyan Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Angiotensin Analogs with Divergent Bias Stabilize Distinct Receptor Conformations

(2019) Laura M. Wingler et al.   CELL

An online resource for GPCR structure determination and analysis

(2019) Christian Munk et al.   NATURE METHODS

NMR analysis of GPCR conformational landscapes and dynamics

(2019) Marina Casiraghi et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs

(2019) Timo W.M. De Groof et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Nanobodies detecting and modulating GPCRs outside in and inside out

(2019) Raimond Heukers et al.   CURRENT OPINION IN CELL BIOLOGY

Molecular basis for high-affinity agonist binding in GPCRs

(2019) Tony Warne et al.   SCIENCE

The role of NMR spectroscopy in mapping the conformational landscape of GPCRs

(2019) Mark J Bostock et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

High pressure shifts the β1-adrenergic receptor to the active conformation in the absence of G protein

(2019) Layara Akemi Abiko et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs

(2019) Laurens Kooijman et al.   JOURNAL OF BIOMOLECULAR NMR

Cleaning up stalled ribosome-translocon complexes with ufmylation

(2019) Adele Xu et al.   CELL RESEARCH

Mini G protein probes for active G protein–coupled receptors (GPCRs) in live cells

(2018) Qingwen Wan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Production of isotope-labeled proteins in insect cells for NMR

(2018) Bastian Franke et al.   JOURNAL OF BIOMOLECULAR NMR

Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR

(2018) Yutaro Shiraishi et al.   Nature Communications

Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein

(2018) Javier García-Nafría et al.   eLife

Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go

(2018) Javier García-Nafría et al.   NATURE

Crystal structure of rhodopsin in complex with a mini-Go sheds light on the principles of G protein selectivity

(2018) Ching-Ju Tsai et al.   Science Advances

GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures

(2018) Ichio Shimada et al.   NATURE REVIEWS DRUG DISCOVERY

Trends in GPCR drug discovery: new agents, targets and indications

(2017) Alexander S. Hauser et al.   NATURE REVIEWS DRUG DISCOVERY

Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor

(2017) Andras S. Solt et al.   Nature Communications

Automated evaluation of quaternary structures from protein crystal structures

(2017) Jose M. Duarte et al.   Acta Crystallographica A-Foundation and Advances

Mini-G proteins: Novel tools for studying GPCRs in their active conformation

(2017) Rony Nehmé et al.   PLoS One

Structure of the adenosine A2A receptor bound to an engineered G protein

(2016) Byron Carpenter et al.   NATURE

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation

(2016) Dean P. Staus et al.   NATURE

Engineering a minimal G protein to facilitate crystallisation of G protein-coupled receptors in their active conformation

(2016) Byron Carpenter et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

(2015) Agnieszka Sitarska et al.   JOURNAL OF BIOMOLECULAR NMR

Stabilization of G protein-coupled receptors by point mutations

(2015) Franziska M. Heydenreich et al.   Frontiers in Pharmacology

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody

(2013) Aaron M. Ring et al.   NATURE

A crystal clear solution for determining G-protein-coupled receptor structures

(2012) Christopher G. Tate   TRENDS IN BIOCHEMICAL SCIENCES

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

Nanobody stabilization of G protein-coupled receptor conformational states

(2011) Jan Steyaert et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineering an Ultra-Thermostable β1-Adrenoceptor

(2011) Jennifer L. Miller et al.   JOURNAL OF MOLECULAR BIOLOGY

The structural basis for agonist and partial agonist action on a β1-adrenergic receptor

(2011) Tony Warne et al.   NATURE

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Solution-State NMR Spectroscopy of a Seven-Helix Transmembrane Protein Receptor: Backbone Assignment, Secondary Structure, and Dynamics

(2008) Antoine Gautier et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structure of a β1-adrenergic G-protein-coupled receptor

(2008) Tony Warne et al.   NATURE

Conformational thermostabilization of the  1-adrenergic receptor in a detergent-resistant form

(2008) M. J. Serrano-Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA