Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A 2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   CELL

Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR

(2018) Yutaro Shiraishi et al.   Nature Communications

Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor

(2017) Andras S. Solt et al.   Nature Communications

Ligand modulation of sidechain dynamics in a wild-type human GPCR

(2017) Lindsay D Clark et al.   eLife

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

Conductance of P2X4purinergic receptor is determined by conformational equilibrium in the transmembrane region

(2016) Yuichi Minato et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

β 2 -Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop

(2016) Matthew T. Eddy et al.   STRUCTURE

Successful Strategies to Determine High-Resolution Structures of GPCRs

(2016) Jin Xiang et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

In-Membrane Chemical Modification (IMCM) for Site-Specific Chromophore Labeling of GPCRs

(2015) Lukas Sušac et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Identification of a Conformational Equilibrium That Determines the Efficacy and Functional Selectivity of the μ-Opioid Receptor

(2015) Junya Okude et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

(2015) Agnieszka Sitarska et al.   JOURNAL OF BIOMOLECULAR NMR

An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts

(2015) Christian Opitz et al.   JOURNAL OF BIOMOLECULAR NMR

Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

(2015) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

Propagation of conformational changes during μ-opioid receptor activation

(2015) Rémy Sounier et al.   NATURE

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The role of protein dynamics in GPCR function: insights from the β2AR and rhodopsin

(2014) Aashish Manglik et al.   CURRENT OPINION IN CELL BIOLOGY

β2-Adrenergic Receptor Activation by Agonists Studied with19F NMR Spectroscopy

(2013) Reto Horst et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Perdeuteration and methyl-selective 1H, 13C-labeling by using a Kluyveromyces lactis expression system

(2013) Mayumi Miyazawa-Onami et al.   JOURNAL OF BIOMOLECULAR NMR

Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids

(2013) Yohei Miyanoiri et al.   JOURNAL OF BIOMOLECULAR NMR

The Role of Ligands on the Equilibria Between Functional States of a G Protein-Coupled Receptor

(2013) Tae Hun Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells

(2012) Satoshi Kubo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Functional Equilibrium of the KcsA Structure Revealed by NMR

(2012) Shunsuke Imai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Role of Detergents in Conformational Exchange of a G Protein-coupled Receptor

(2012) Ka Young Chung et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An Economical Method for Production of 2H,13CH3-Threonine for Solution NMR Studies of Large Protein Complexes: Application to the 670 kDa Proteasome

(2012) Algirdas Velyvis et al.   PLoS One

Biased Signaling Pathways in  2-Adrenergic Receptor Characterized by 19F-NMR

(2012) J. J. Liu et al.   SCIENCE

Efficacy of the β2-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

(2012) Yutaka Kofuku et al.   Nature Communications

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

Trends in the exploitation of novel drug targets

(2011) Mathias Rask-Andersen et al.   NATURE REVIEWS DRUG DISCOVERY

Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

(2010) Pierre Gans et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A simple strategy for 13C,1H labeling at the Ile-γ2 methyl position in highly deuterated proteins

(2010) Amy M. Ruschak et al.   JOURNAL OF BIOMOLECULAR NMR

Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

(2010) Michael P. Bokoch et al.   NATURE

An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein

(2009) Isabel Ayala et al.   JOURNAL OF BIOMOLECULAR NMR