Advances in the study of GPCRs by 19F NMR

Delineating the conformational landscape of the adenosine A2A receptor during G protein coupling

(2021) Shuya Kate Huang et al.   CELL

Conformational plasticity of ligand-bound and ternary GPCR complexes studied by 19F NMR of the β1-adrenergic receptor

(2020) J. Niclas Frei et al.   Nature Communications

Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR

(2020) Takuya Mizumura et al.   Science Advances

The precious fluorine on the ring: fluorine NMR for biological systems

(2020) Andras Boeszoermenyi et al.   JOURNAL OF BIOMOLECULAR NMR

Use of paramagnetic 19F NMR to monitor domain movement in a glutamate transporter homolog

(2020) Yun Huang et al.   Nature Chemical Biology

Probing the Conformational States of Neurotensin Receptor 1 Variants by NMR Site‐Directed Methyl Labeling

(2020) Inguna Goba et al.   CHEMBIOCHEM

Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics

(2019) Andras Boeszoermenyi et al.   NATURE METHODS

Detergent- and phospholipid-based reconstitution systems have differential effects on constitutive activity of G protein–coupled receptors

(2019) Dean P Staus et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

2-Fluorotyrosine is a valuable but understudied amino acid for protein-observed 19F NMR

(2019) Peter D. Ycas et al.   JOURNAL OF BIOMOLECULAR NMR

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A 2A Adenosine Receptor

(2018) Matthew T. Eddy et al.   CELL

Mixed Fluorotryptophan Substitutions at the Same Residue Expand the Versatility of 19 F Protein NMR Spectroscopy

(2018) Calem Kenward et al.   CHEMISTRY-A EUROPEAN JOURNAL

Expression, Purification and Characterization of the Human Cannabinoid 1 Receptor

(2018) Srikrishnan Mallipeddi et al.   Scientific Reports

A2A adenosine receptor functional states characterized by 19F-NMR

(2018) Lukas Sušac et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Application of geometric approximation to the CPMG experiment: Two- and three-site exchange

(2017) Fa-An Chao et al.   JOURNAL OF MAGNETIC RESONANCE

Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids

(2017) Jordan K. Villa et al.   MOLECULES

Biophysical and functional characterization of the human olfactory receptor OR1A1 expressed in a mammalian inducible cell line

(2017) Christine Belloir et al.   PROTEIN EXPRESSION AND PURIFICATION

The role of dimer asymmetry and protomer dynamics in enzyme catalysis

(2017) Tae Hun Kim et al.   SCIENCE

Ligand modulation of sidechain dynamics in a wild-type human GPCR

(2017) Lindsay D Clark et al.   eLife

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

(2016) Ralf-Bernhardt Rues et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Geometric Approximation: A New Computational Approach To Characterize Protein Dynamics from NMR Adiabatic Relaxation Dispersion Experiments

(2016) Fa-An Chao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Prediction of 19F NMR Chemical Shifts in Labeled Proteins: Computational Protocol and Case Study

(2016) William C. Isley et al.   MOLECULAR PHARMACEUTICS

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

Covalently circularized nanodiscs for studying membrane proteins and viral entry

(2016) Mahmoud L Nasr et al.   NATURE METHODS

β 2 -Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop

(2016) Matthew T. Eddy et al.   STRUCTURE

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

A comparison of chemical shift sensitivity of trifluoromethyl tags: optimizing resolution in 19F NMR studies of proteins

(2015) Libin Ye et al.   JOURNAL OF BIOMOLECULAR NMR

Protein-Observed Fluorine NMR: A Bioorthogonal Approach for Small Molecule Discovery

(2015) Keith E. Arntson et al.   JOURNAL OF MEDICINAL CHEMISTRY

Fluorinated Aromatic Amino Acids Are Sensitive 19F NMR Probes for Bromodomain-Ligand Interactions

(2014) Neeraj K. Mishra et al.   ACS Chemical Biology

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

(2014) Ralf-Bernhardt Rues et al.   BIOLOGICAL CHEMISTRY

Expression, stabilization and purification of membrane proteins via diverse protein synthesis systems and detergents involving cell-free associated with self-assembly peptide surfactants

(2014) Xuan Zheng et al.   BIOTECHNOLOGY ADVANCES

The Role of Ligands on the Equilibria Between Functional States of a G Protein-Coupled Receptor

(2013) Tae Hun Kim et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers

(2013) A. Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Membrane phospholipid bilayer as a determinant of monoacylglycerol lipase kinetic profile and conformational repertoire

(2013) Mahmoud L. Nasr et al.   PROTEIN SCIENCE

Studying “Invisible” Excited Protein States in Slow Exchange with a Major State Conformation

(2012) Pramodh Vallurupalli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biased Signaling Pathways in  2-Adrenergic Receptor Characterized by 19F-NMR

(2012) J. J. Liu et al.   SCIENCE

Organic fluorine compounds: a great opportunity for enhanced materials properties

(2011) Ricarda Berger et al.   CHEMICAL SOCIETY REVIEWS

Two distinct conformations of helix 6 observed in antagonist-bound structures of a  1-adrenergic receptor

(2011) R. Moukhametzianov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Current applications of 19F NMR to studies of protein structure and dynamics

(2011) Julianne L. Kitevski-LeBlanc et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Optimizing 19F NMR protein spectroscopy by fractional biosynthetic labeling

(2010) Julianne L. Kitevski-LeBlanc et al.   JOURNAL OF BIOMOLECULAR NMR

19F NMR chemical shift prediction with fluorine fingerprint descriptor

(2010) Anna Vulpetti et al.   JOURNAL OF FLUORINE CHEMISTRY

In vivoIncorporation of Unnatural Amino Acids to Probe Structure, Dynamics, and Ligand Binding in a Large Protein by Nuclear Magnetic Resonance Spectroscopy

(2008) Susan E. Cellitti et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Heterologous GPCR Expression: A Bottleneck to Obtaining Crystal Structures

(2007) Emily C. McCusker et al.   BIOTECHNOLOGY PROGRESS