Structural and functional analysis of the promiscuous AcrB and AdeB efflux pumps suggests different drug binding mechanisms

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Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time

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Interactions of a Bacterial RND Transporter with a Transmembrane Small Protein in a Lipid Environment

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Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex

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Holo-like and Druggable Protein Conformations from Enhanced Sampling of Binding Pocket Volume and Shape

(2019) Andrea Basciu et al.   Journal of Chemical Information and Modeling

Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump

(2019) Chih-Chia Su et al.   mBio

Structures of the wild-type MexAB–OprM tripartite pump reveal its complex formation and drug efflux mechanism

(2019) Kenta Tsutsumi et al.   Nature Communications

AcrB: a mean, keen, drug efflux machine

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Multidrug efflux pumps: structure, function and regulation

(2018) Dijun Du et al.   NATURE REVIEWS MICROBIOLOGY

Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition

(2018) Martijn Zwama et al.   Nature Communications

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The hydrophobic trap—the Achilles heel of RND efflux pumps

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Synergy between Active Efflux and Outer Membrane Diffusion Defines Rules of Antibiotic Permeation into Gram-Negative Bacteria

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An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump

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Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives

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The assembly and disassembly of the AcrAB-TolC three-component multidrug efflux pump

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Properties of AdeABC and AdeIJK Efflux Systems of Acinetobacter baumannii Compared with Those of the AcrAB-TolC System of Escherichia coli

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Crystal Structure of the Neisseria gonorrhoeae MtrD Inner Membrane Multidrug Efflux Pump

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Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB

(2014) Thomas Eicher et al.   eLife

How good are my data and what is the resolution?

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RND-Type Efflux Pumps in Multidrug-Resistant Clinical Isolates of Acinetobacter baumannii: Major Role for AdeABC Overexpression and AdeRS Mutations

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Drug Uptake Pathways of Multidrug Transporter AcrB Studied by Molecular Simulations and Site-Directed Mutagenesis Experiments

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OmpA Is the Principal Nonspecific Slow Porin of Acinetobacter baumannii

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Multidrug binding properties of the AcrB efflux pump characterized by molecular dynamics simulations

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Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop

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Effect of the F610A Mutation on Substrate Extrusion in the AcrB Transporter: Explanation and Rationale by Molecular Dynamics Simulations

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Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

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XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

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Overexpression of Resistance-Nodulation-Cell Division Pump AdeFGH Confers Multidrug Resistance in Acinetobacter baumannii

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Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport

(2010) Feng Long et al.   NATURE

MolProbity: all-atom structure validation for macromolecular crystallography

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Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB

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Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

(2008) Irith Wiegand et al.   Nature Protocols