AcrB: a mean, keen, drug efflux machine

Crystal Structures of Membrane Transporter MmpL3, an Anti-TB Drug Target

(2019) Bing Zhang et al.   CELL

The rise and spread of mcr plasmid-mediated polymyxin resistance

(2019) Sue C. Nang et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Definitions and guidelines for research on antibiotic persistence

(2019) Nathalie Q. Balaban et al.   NATURE REVIEWS MICROBIOLOGY

MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine

(2019) Chih-Chia Su et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer

(2019) Sophie Nolivos et al.   SCIENCE

Permeability barriers of Gram-negative pathogens

(2019) Helen I. Zgurskaya et al.   Annals of the New York Academy of Sciences

Structures of the wild-type MexAB–OprM tripartite pump reveal its complex formation and drug efflux mechanism

(2019) Kenta Tsutsumi et al.   Nature Communications

Water-mediated interactions enable smooth substrate transport in a bacterial efflux pump

(2018) Attilio Vittorio Vargiu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

New insights into the structural and functional involvement of the gate loop in AcrB export activity

(2018) Abdessamad Ababou   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A New Critical Conformational Determinant of Multidrug Efflux by an MFS Transporter

(2018) Elia Zomot et al.   JOURNAL OF MOLECULAR BIOLOGY

Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis

(2018) Evelina Tacconelli et al.   LANCET INFECTIOUS DISEASES

Multidrug efflux pumps: structure, function and regulation

(2018) Dijun Du et al.   NATURE REVIEWS MICROBIOLOGY

Structure and mechanism of bacterial tripartite efflux pumps

(2018) Arthur Neuberger et al.   RESEARCH IN MICROBIOLOGY

RND transporters in the living world

(2018) Hiroshi Nikaido   RESEARCH IN MICROBIOLOGY

Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition

(2018) Martijn Zwama et al.   Nature Communications

Molecular Determinants of the Promiscuity of MexB and MexY Multidrug Transporters of Pseudomonas aeruginosa

(2018) Venkata K. Ramaswamy et al.   Frontiers in Microbiology

Structures of human Patched and its complex with native palmitoylated sonic hedgehog

(2018) Xiaofeng Qi et al.   NATURE

Structure of the EmrE multidrug transporter and its use for inhibitor peptide design

(2018) Victor Ovchinnikov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex

(2018) Xiaofeng Qi et al.   SCIENCE

Identification and characterization of carbapenem binding sites within the RND-transporter AcrB

(2018) Alessio Atzori et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Structure and activity of lipid bilayer within a membrane-protein transporter

(2018) Weihua Qiu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Constant pH Molecular Dynamics Reveals How Proton Release Drives the Conformational Transition of a Transmembrane Efflux Pump

(2017) Zhi Yue et al.   Journal of Chemical Theory and Computation

Switch Loop Flexibility Affects Substrate Transport of the AcrB Efflux Pump

(2017) Reinke T. Müller et al.   JOURNAL OF MOLECULAR BIOLOGY

The age of the hominin fossils from Jebel Irhoud, Morocco, and the origins of the Middle Stone Age

(2017) Daniel Richter et al.   NATURE

Evidence for early life in Earth’s oldest hydrothermal vent precipitates

(2017) Matthew S. Dodd et al.   NATURE

Crystal structures of theBurkholderia multivoranshopanoid transporter HpnN

(2017) Nitin Kumar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

3.3 Å structure of Niemann–Pick C1 protein reveals insights into the function of the C-terminal luminal domain in cholesterol transport

(2017) Xiaochun Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP

(2017) Anne Nöll et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures and transport dynamics of a Campylobacter jejuni multidrug efflux pump

(2017) Chih-Chia Su et al.   Nature Communications

Structural basis for xenobiotic extrusion by eukaryotic MATE transporter

(2017) Hirotake Miyauchi et al.   Nature Communications

Dynamics of Intact MexAB-OprM Efflux Pump: Focusing on the MexA-OprM Interface

(2017) Cesar A. López et al.   Scientific Reports

An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump

(2017) Zhao Wang et al.   eLife

Structure of the MacAB–TolC ABC-type tripartite multidrug efflux pump

(2017) Anthony W. P. Fitzpatrick et al.   Nature Microbiology

Molecular Rationale behind the Differential Substrate Specificity of Bacterial RND Multi-Drug Transporters

(2017) Venkata Krishnan Ramaswamy et al.   Scientific Reports

Insights into the Inhibitory Mechanism of D13-9001 to the Multidrug Transporter AcrB through Molecular Dynamics Simulations

(2016) Zhicheng Zuo et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells

(2016) Yingying Pu et al.   MOLECULAR CELL

Mechanistic diversity in ATP-binding cassette (ABC) transporters

(2016) Kaspar P Locher   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Antibiotic treatment enhances the genome-wide mutation rate of target cells

(2016) Hongan Long et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives

(2016) Hanno Sjuts et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protonation-dependent conformational dynamics of the multidrug transporter EmrE

(2016) Reza Dastvan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pseudoatomic Structure of the Tripartite Multidrug Efflux Pump AcrAB-TolC Reveals the Intermeshing Cogwheel-like Interaction between AcrA and TolC

(2016) Hyeongseop Jeong et al.   STRUCTURE

Transport of lipophilic carboxylates is mediated by transmembrane helix 2 in multidrug transporter AcrB

(2016) Christine Oswald et al.   Nature Communications

Tripartite assembly of RND multidrug efflux pumps

(2016) Laetitia Daury et al.   Nature Communications

A diverse intrinsic antibiotic resistome from a cave bacterium

(2016) Andrew C. Pawlowski et al.   Nature Communications

Mechanistic diversity in ATP-binding cassette (ABC) transporters

(2016) Kaspar P Locher   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Prehistory of Antibiotic Resistance

(2016) Julie Perry et al.   Cold Spring Harbor Perspectives in Medicine

Editorial: Bad Bugs in the XXIst Century: Resistance Mediated by Multi-Drug Efflux Pumps in Gram-Negative Bacteria

(2016) Attilio V. Vargiu et al.   Frontiers in Microbiology

Structures of Gate Loop Variants of the AcrB Drug Efflux Pump Bound by Erythromycin Substrate

(2016) Abdessamad Ababou et al.   PLoS One

Mechanisms of drug resistance: quinolone resistance

(2015) David C. Hooper et al.   Annals of the New York Academy of Sciences

A Fluorescent Microplate Assay Quantifies Bacterial Efflux and Demonstrates Two Distinct Compound Binding Sites in AcrB

(2015) Ramkumar Iyer et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Plasmid-Mediated OqxAB Is an Important Mechanism for Nitrofurantoin Resistance in Escherichia coli

(2015) Pak-Leung Ho et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

The assembly and disassembly of the AcrAB-TolC three-component multidrug efflux pump

(2015) Reinke Tobias Müller et al.   BIOLOGICAL CHEMISTRY

Substrate-bound structure of the E. coli multidrug resistance transporter MdfA

(2015) Jie Heng et al.   CELL RESEARCH

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

(2015) Xian-Zhi Li et al.   CLINICAL MICROBIOLOGY REVIEWS

AcrB-AcrA Fusion Proteins That Act as Multidrug Efflux Transporters

(2015) Katsuhiko Hayashi et al.   JOURNAL OF BACTERIOLOGY

Structure of the Tripartite Multidrug Efflux Pump AcrAB-TolC Suggests an Alternative Assembly Mode

(2015) Jin-Sik Kim et al.   MOLECULES AND CELLS

A new antibiotic kills pathogens without detectable resistance

(2015) Losee L. Ling et al.   NATURE

Global trends in antimicrobial use in food animals

(2015) Thomas P. Van Boeckel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Homologs of the Acinetobacter baumannii AceI Transporter Represent a New Family of Bacterial Multidrug Efflux Systems

(2015) Karl A. Hassan et al.   mBio

In vitro transport activity of the fully assembled MexAB-OprM efflux pump from Pseudomonas aeruginosa

(2015) Alice Verchère et al.   Nature Communications

RND-type drug efflux pumps from Gram-negative bacteria: molecular mechanism and inhibition

(2015) Henrietta Venter et al.   Frontiers in Microbiology

The ins and outs of RND efflux pumps in Escherichia coli

(2015) João Anes et al.   Frontiers in Microbiology

Structural basis of RND-type multidrug exporters

(2015) Akihito Yamaguchi et al.   Frontiers in Microbiology

Switch-Loop Flexibility Affects Transport of Large Drugs by the Promiscuous AcrB Multidrug Efflux Transporter

(2014) Hi-jea Cha et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Molecular Mechanism of MBX2319 Inhibition of Escherichia coli AcrB Multidrug Efflux Pump and Comparison with Other Inhibitors

(2014) Attilio V. Vargiu et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Expression of homologous RND efflux pump genes is dependent upon AcrB expression: implications for efflux and virulence inhibitor design

(2014) Jessica M. A. Blair et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

β-Lactam Selectivity of Multidrug Transporters AcrB and AcrD Resides in the Proximal Binding Pocket

(2014) Naoki Kobayashi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of the AcrAB–TolC multidrug efflux pump

(2014) Dijun Du et al.   NATURE

Molecular mechanisms of antibiotic resistance

(2014) Jessica M. A. Blair et al.   NATURE REVIEWS MICROBIOLOGY

Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB

(2014) Thomas Eicher et al.   eLife

Protoporphyrin (PPIX) efflux by the MacAB-TolC pump inEscherichia coli

(2014) Evelyne Turlin et al.   MicrobiologyOpen

Characterization of a Novel Pyranopyridine Inhibitor of the AcrAB Efflux Pump of Escherichia coli

(2013) Timothy J. Opperman et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Role of a Conserved Residue R780 in Escherichia coli Multidrug Transporter AcrB

(2013) Linliang Yu et al.   BIOCHEMISTRY

Some Ligands Enhance the Efflux of Other Ligands by the Escherichia coli Multidrug Pump AcrB

(2013) Alfred D. Kinana et al.   BIOCHEMISTRY

MacA, a Periplasmic Membrane Fusion Protein of the Macrolide Transporter MacAB-TolC, Binds Lipopolysaccharide Core Specifically and with High Affinity

(2013) S. Lu et al.   JOURNAL OF BACTERIOLOGY

Structural basis for the inhibition of bacterial multidrug exporters

(2013) Ryosuke Nakashima et al.   NATURE

Identifying producers of antibacterial compounds by screening for antibiotic resistance

(2013) Maulik N Thaker et al.   NATURE BIOTECHNOLOGY

Ribosome-targeting antibiotics and mechanisms of bacterial resistance

(2013) Daniel N. Wilson   NATURE REVIEWS MICROBIOLOGY

Structures of a Na+-coupled, substrate-bound MATE multidrug transporter

(2013) M. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MOLECULAR DYNAMICS COMPUTER SIMULATIONS OF MULTIDRUG RND EFFLUX PUMPS

(2013) Paolo Ruggerone et al.   Computational and Structural Biotechnology Journal

Different Functions of MdtB and MdtC Subunits in the Heterotrimeric Efflux Transporter MdtB2C Complex of Escherichia coli

(2012) Hong-Suk Kim et al.   BIOCHEMISTRY

Porter domain opening and closing motions in the multi-drug efflux transporter AcrB

(2012) Nadine Fischer et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Selective Protein Synthesis by Ribosomes with a Drug-Obstructed Exit Tunnel

(2012) Krishna Kannan et al.   CELL

Adaptive and Mutational Resistance: Role of Porins and Efflux Pumps in Drug Resistance

(2012) L. Fernandez et al.   CLINICAL MICROBIOLOGY REVIEWS

Identification of Transport Proteins Involved in Free Fatty Acid Efflux in Escherichia coli

(2012) R. M. Lennen et al.   JOURNAL OF BACTERIOLOGY

New Substrates on the Block: Clinically Relevant Resistances for EmrE and Homologues

(2012) I. Nasie et al.   JOURNAL OF BACTERIOLOGY

Assembly and Channel Opening of Outer Membrane Protein in Tripartite Drug Efflux Pumps of Gram-negative Bacteria

(2012) Yongbin Xu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Multidrug binding properties of the AcrB efflux pump characterized by molecular dynamics simulations

(2012) A. V. Vargiu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistance

(2012) E. C. Hobbs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop

(2012) T. Eicher et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Shared Antibiotic Resistome of Soil Bacteria and Human Pathogens

(2012) K. J. Forsberg et al.   SCIENCE

Undecided membrane proteins insert in random topologies. Up, down and sideways: it does not really matter

(2012) Shimon Schuldiner   TRENDS IN BIOCHEMICAL SCIENCES

Exploiting bacterial DNA gyrase as a drug target: current state and perspectives

(2011) Frédéric Collin et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Analysis of AcrB and AcrB/DARPin ligand complexes by LILBID MS

(2011) Lorenz Brandstätter et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Folding of AcrB Subunit Precedes Trimerization

(2011) Wei Lu et al.   JOURNAL OF MOLECULAR BIOLOGY

Functionality Mapping on Internal Surfaces of Multidrug Transporter AcrB Based on Molecular Theory of Solvation: Implications for Drug Efflux Pathway

(2011) Takashi Imai et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Effect of the F610A Mutation on Substrate Extrusion in the AcrB Transporter: Explanation and Rationale by Molecular Dynamics Simulations

(2011) Attilio V. Vargiu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli

(2011) Chih-Chia Su et al.   NATURE

Structure and function of a membrane component SecDF that enhances protein export

(2011) Tomoya Tsukazaki et al.   NATURE

Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

(2011) Ryosuke Nakashima et al.   NATURE

Antiparallel EmrE exports drugs by exchanging between asymmetric structures

(2011) Emma A. Morrison et al.   NATURE

Antibiotic resistance is ancient

(2011) Vanessa M. D’Costa et al.   NATURE

Evolutionary paths to antibiotic resistance under dynamically sustained drug selection

(2011) Erdal Toprak et al.   NATURE GENETICS

AcrB Trimer Stability and Efflux Activity, Insight from Mutagenesis Studies

(2011) Linliang Yu et al.   PLoS One

Structures of sequential open states in a symmetrical opening transition of the TolC exit duct

(2011) Xue-Yuan Pei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Electrical Spiking in Escherichia coli Probed with a Fluorescent Voltage-Indicating Protein

(2011) Joel M. Kralj et al.   SCIENCE

Mechanism and Function of the Outer Membrane Channel TolC in Multidrug Resistance and Physiology of Enterobacteria

(2011) Helen I. Zgurskaya et al.   Frontiers in Microbiology

Optimized Nile Red Efflux Assay of AcrAB-TolC Multidrug Efflux System Shows Competition between Substrates

(2010) J. A. Bohnert et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Kinetic Parameters of Efflux of Penicillins by the Multidrug Efflux Transporter AcrAB-TolC of Escherichia coli

(2010) S. P. Lim et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Phenotypic Landscape of a Bacterial Cell

(2010) Robert J. Nichols et al.   CELL

Asymmetric ATP Hydrolysis Cycle of the Heterodimeric Multidrug ABC Transport Complex TmrAB fromThermus thermophilus

(2010) Ariane Zutz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dissection of β-barrel outer membrane protein assembly pathways through characterizing BamA POTRA 1 mutants of Escherichia coli

(2010) Drew Bennion et al.   MOLECULAR MICROBIOLOGY

Structure of a cation-bound multidrug and toxic compound extrusion transporter

(2010) Xiao He et al.   NATURE

How antibiotics kill bacteria: from targets to networks

(2010) Michael A. Kohanski et al.   NATURE REVIEWS MICROBIOLOGY

Antibiotic resistance and its cost: is it possible to reverse resistance?

(2010) Dan I. Andersson et al.   NATURE REVIEWS MICROBIOLOGY

Call of the wild: antibiotic resistance genes in natural environments

(2010) Heather K. Allen et al.   NATURE REVIEWS MICROBIOLOGY

Mechanism of recognition of compounds of diverse structures by the multidrug efflux pump AcrB of Escherichia coli

(2010) Y. Takatsuka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

oqxAB Encoding a Multidrug Efflux Pump in Human Clinical Isolates of Enterobacteriaceae

(2009) H. B. Kim et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Crucial Role of Asp408 in the Proton Translocation Pathway of Multidrug Transporter AcrB: Evidence from Site-Directed Mutagenesis and Carbodiimide Labeling

(2009) Markus A. Seeger et al.   BIOCHEMISTRY

Drug transport mechanism of the AcrB efflux pump

(2009) Klaas M. Pos   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Biopython: freely available Python tools for computational molecular biology and bioinformatics

(2009) P. J. A. Cock et al.   BIOINFORMATICS

The natural history of antibiotics

(2009) Jon Clardy et al.   CURRENT BIOLOGY

Hopanoids Play a Role in Membrane Integrity and pH Homeostasis in Rhodopseudomonas palustris TIE-1

(2009) P. V. Welander et al.   JOURNAL OF BACTERIOLOGY

Multidrug Efflux Pump MdtBC of Escherichia coli Is Active Only as a B2C Heterotrimer

(2009) H.-S. Kim et al.   JOURNAL OF BACTERIOLOGY

A coordinated network of transporters with overlapping specificities provides a robust survival strategy

(2009) N. Tal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The assembled structure of a complete tripartite bacterial multidrug efflux pump

(2009) M. F. Symmons et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kinetic behavior of the major multidrug efflux pump AcrB of Escherichia coli

(2009) K. Nagano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Directed Mutagenesis Reveals Amino Acid Residues in the Escherichia coli RND Efflux Pump AcrB That Confer Macrolide Resistance

(2008) C. Wehmeier et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Multidrug efflux transporters in the MATE family

(2008) Teruo Kuroda et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

The AcrB Efflux Pump: Conformational Cycling and Peristalsis Lead to Multidrug Resistance

(2008) Markus Seeger et al.   CURRENT DRUG TARGETS

Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB

(2008) J. A. Bohnert et al.   JOURNAL OF BACTERIOLOGY

Covalently Linked Trimer of the AcrB Multidrug Efflux Pump Provides Support for the Functional Rotating Mechanism

(2008) Y. Takatsuka et al.   JOURNAL OF BACTERIOLOGY

MacAB Is Involved in the Secretion of Escherichia coli Heat-Stable Enterotoxin II

(2008) H. Yamanaka et al.   JOURNAL OF BACTERIOLOGY

Assembly and Channel Opening in a Bacterial Drug Efflux Machine

(2008) Vassiliy N. Bavro et al.   MOLECULAR CELL

Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB

(2008) Markus A Seeger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Bacteria Subsisting on Antibiotics

(2008) G. Dantas et al.   SCIENCE