The Application of Brain Organoid Technology in Stroke Research: Challenges and Prospects

Modeling of Hypoxic Brain Injury through 3D Human Neural Organoids

(2021) Min Soo Kim et al.   Cells

Cerebral organoids transplantation improves neurological motor function in rat brain injury

(2020) Zhi Wang et al.   CNS Neuroscience & Therapeutics

Modeling and Targeting Alzheimer’s Disease With Organoids

(2020) Angelos Papaspyropoulos et al.   Frontiers in Pharmacology

In vitro modeling of the neurovascular unit: advances in the field

(2020) Aditya Bhalerao et al.   Fluids and Barriers of the CNS

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

(2020) Goodwell Nzou et al.   Scientific Reports

Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids

(2020) Takahiro Kitahara et al.   Stem Cell Reports

Clinical Trials of Stem Cell Therapy for Cerebral Ischemic Stroke

(2020) Masahito Kawabori et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Human cerebral organoids establish subcortical projections in the mouse brain after transplantation

(2020) Xin Dong et al.   MOLECULAR PSYCHIATRY

Generation of Functional Human 3D Cortico-Motor Assembloids

(2020) Jimena Andersen et al.   CELL

Vascular Smooth Muscle Cell Derived from IPS Cell of Moyamoya Disease - Comparative Characterization with Endothelial Cell Transcriptome

(2020) Kikutaro Tokairin et al.   Journal of Stroke & Cerebrovascular Diseases

Generation of an integration-free induced pluripotent stem cell line (UNC001-A) from blood of a healthy individual

(2020) Sarahi G. Molina et al.   Stem Cell Research

Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells

(2019) Chen Ling et al.   Protein & Cell

Large animals in neurointerventional research: A systematic review on models, techniques and their application in endovascular procedures for stroke, aneurysms and vascular malformations

(2019) Andrea M Herrmann et al.   JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Human 3D cellular model of hypoxic brain injury of prematurity

(2019) Anca M. Pașca et al.   NATURE MEDICINE

Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids

(2019) Erin M. Boisvert et al.   Cell Death & Disease

Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins

(2019) Hyosung Kim et al.   Stem Cell Reports

Analysis of Synapses in Cerebral Organoids

(2019) Abraam M. Yakoub et al.   CELL TRANSPLANTATION

Distinct Vulnerability and Resilience of Human Neuroprogenitor Subtypes in Cerebral Organoid Model of Prenatal Hypoxic Injury

(2019) Nicolas Daviaud et al.   Frontiers in Cellular Neuroscience

Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues

(2019) Liqing Song et al.   Scientific Reports

Engineering of human brain organoids with a functional vascular-like system

(2019) Bilal Cakir et al.   NATURE METHODS

Stem Cells as an Emerging Paradigm in Stroke 4

(2019) Johannes Boltze et al.   STROKE

Organotypic Neurovascular Models: Past Results and Future Directions

(2019) Daniel A. Balikov et al.   TRENDS IN MOLECULAR MEDICINE

Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells

(2019) Philipp Wörsdörfer et al.   Scientific Reports

Patient-Specific iPSC Model of a Genetic Vascular Dementia Syndrome Reveals Failure of Mural Cells to Stabilize Capillary Structures

(2019) Joseph Kelleher et al.   Stem Cell Reports

Blood vessel formation in cerebral organoids formed from human embryonic stem cells

(2019) Onju Ham et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Modelling heme-mediated brain injury associated with cerebral malaria in human brain cortical organoids

(2019) Adriana Harbuzariu et al.   Scientific Reports

Cerebral Organoids Repair Ischemic Stroke Brain Injury

(2019) Shu-Na Wang et al.   Translational Stroke Research

3D bioprinting of tissues and organs for regenerative medicine

(2018) Sanjairaj Vijayavenkataraman et al.   ADVANCED DRUG DELIVERY REVIEWS

Heart Disease and Stroke Statistics—2018 Update: A Report From the American Heart Association

(2018) Emelia J. Benjamin et al.   CIRCULATION

Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo

(2018) Reem Basuodan et al.   JOURNAL OF ANATOMY

An in vivo model of functional and vascularized human brain organoids

(2018) Abed AlFatah Mansour et al.   NATURE BIOTECHNOLOGY

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

(2018) Yuan-Ta Lin et al.   NEURON

Generation of human vascularized brain organoids

(2018) Missy T. Pham et al.   NEUROREPORT

Intrathecal Injection of Allogenic Bone Marrow-Derived Mesenchymal Stromal Cells in Treatment of Patients with Severe Ischemic Stroke: Study Protocol for a Randomized Controlled Observer-Blinded Trial

(2018) Lingna Deng et al.   Translational Stroke Research

Applications of human brain organoids to clinical problems

(2018) H. Isaac Chen et al.   DEVELOPMENTAL DYNAMICS

Generation and post-injury integration of human spinal cord neural stem cells

(2018) Hiromi Kumamaru et al.   NATURE METHODS

Generation and assembly of human brain region–specific three-dimensional cultures

(2018) Steven A. Sloan et al.   Nature Protocols

Human Cortical Neuron Generation Using Cell Reprogramming: A Review of Recent Advances

(2018) Amy McCaughey-Chapman et al.   STEM CELLS AND DEVELOPMENT

Engrafted parenchymal brain macrophages differ from microglia in transcriptome, chromatin landscape and response to challenge

(2018) Anat Shemer et al.   Nature Communications

Microglia innately develop within cerebral organoids

(2018) Paul R. Ormel et al.   Nature Communications

Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation

(2017) Sara Nolbrant et al.   Nature Protocols

The use of brain organoids to investigate neural development and disease

(2017) Elizabeth Di Lullo et al.   NATURE REVIEWS NEUROSCIENCE

Magnetic resonance imaging-three-dimensional printing technology fabricates customized scaffolds for brain tissue engineering

(2017) Ming-liang Zhao et al.   Neural Regeneration Research

Pathogenic mechanisms following ischemic stroke

(2017) Seyed Esmaeil Khoshnam et al.   NEUROLOGICAL SCIENCES

Modeling Physiological Events in 2D vs. 3D Cell Culture

(2017) Kayla Duval et al.   PHYSIOLOGY

Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke

(2017) Ling Wei et al.   PROGRESS IN NEUROBIOLOGY

Disease Modeling in Stem Cell-Derived 3D Organoid Systems

(2017) Devanjali Dutta et al.   TRENDS IN MOLECULAR MEDICINE

Blood-brain-barrier spheroids as an in vitro screening platform for brain-penetrating agents

(2017) Choi-Fong Cho et al.   Nature Communications

Novel Regenerative Therapies Based on Regionally Induced Multipotent Stem Cells in Post-Stroke Brains: Their Origin, Characterization, and Perspective

(2017) Toshinori Takagi et al.   Translational Stroke Research

CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in cerebral organoids derived from iPS cells

(2017) Ping Wang et al.   Molecular Autism

Three-Dimensional Organoid System Transplantation Technologies in Future Treatment of Central Nervous System Diseases

(2017) NaiLi Wei et al.   Stem Cells International

Directed Differentiation of Human Pluripotent Stem Cells to Microglia

(2017) Panagiotis Douvaras et al.   Stem Cell Reports

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Zika Virus Depletes Neural Progenitors in Human Cerebral Organoids through Activation of the Innate Immune Receptor TLR3

(2016) Jason Dang et al.   Cell Stem Cell

Dishing out mini-brains: Current progress and future prospects in brain organoid research

(2016) Iva Kelava et al.   DEVELOPMENTAL BIOLOGY

Neuroprotection in acute stroke: targeting excitotoxicity, oxidative and nitrosative stress, and inflammation

(2016) Ángel Chamorro et al.   LANCET NEUROLOGY

The Brazilian Zika virus strain causes birth defects in experimental models

(2016) Fernanda R. Cugola et al.   NATURE

An iPSC-derived vascular model of Marfan syndrome identifies key mediators of smooth muscle cell death

(2016) Alessandra Granata et al.   NATURE GENETICS

Efficient derivation of microglia-like cells from human pluripotent stem cells

(2016) Julien Muffat et al.   NATURE MEDICINE

CYP3A5 Mediates Effects of Cocaine on Human Neocorticogenesis: Studies using an In Vitro 3D Self-Organized hPSC Model with a Single Cortex-Like Unit

(2016) Chun-Ting Lee et al.   NEUROPSYCHOPHARMACOLOGY

Three-dimensional bioprinting of thick vascularized tissues

(2016) David B. Kolesky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Zika virus impairs growth in human neurospheres and brain organoids

(2016) P. P. Garcez et al.   SCIENCE

Modeling Ischemic Stroke In Vitro: Status Quo and Future Perspectives

(2016) Paul M. Holloway et al.   STROKE

Development of 3D Microvascular Networks Within Gelatin Hydrogels Using Thermoresponsive Sacrificial Microfibers

(2016) Jung Bok Lee et al.   Advanced Healthcare Materials

Generating Late-Onset Human iPSC-Based Disease Models by Inducing Neuronal Age-Related Phenotypes through Telomerase Manipulation

(2016) Elsa Vera et al.   Cell Reports

Human iPSC for Therapeutic Approaches to the Nervous System: Present and Future Applications

(2016) Maria Giuseppina Cefalo et al.   Stem Cells International

Organoids and the genetically encoded self-assembly of embryonic stem cells

(2015) David A. Turner et al.   BIOESSAYS

Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regeneration

(2015) Wenjie Zhang et al.   BIOMATERIALS

FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders

(2015) Jessica Mariani et al.   CELL

It Takes a Village: Constructing the Neurogenic Niche

(2015) Christopher S. Bjornsson et al.   DEVELOPMENTAL CELL

Simultaneous PET/Mri in Stroke: A Case Series

(2015) Peter Werner et al.   JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Spatiotemporal PET Imaging of Dynamic Metabolic Changes After Therapeutic Approaches of Induced Pluripotent Stem Cells, Neuronal Stem Cells, and a Chinese Patent Medicine in Stroke

(2015) H. Zhang et al.   JOURNAL OF NUCLEAR MEDICINE

Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

(2015) J. Gray Camp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human pluripotent stem cell-derived neural constructs for predicting neural toxicity

(2015) Michael P. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

NAMPT as a Therapeutic Target against Stroke

(2015) Pei Wang et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors

(2015) Reuven Edri et al.   Nature Communications

Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells

(2015) Zhong-Wei Du et al.   Nature Communications

Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices

(2015) Nikolce Gjorevski et al.   Scientific Reports

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Generation of cerebral organoids from human pluripotent stem cells

(2014) Madeline A Lancaster et al.   Nature Protocols

CORTECON: A Temporal Transcriptome Analysis of In Vitro Human Cerebral Cortex Development from Human Embryonic Stem Cells

(2014) Joyce van de Leemput et al.   NEURON

Bioengineered functional brain-like cortical tissue

(2014) M. D. Tang-Schomer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

iPSC Transplantation Increases Regeneration and Functional Recovery After Ischemic Stroke in Neonatal Rats

(2014) Monica J. Chau et al.   STEM CELLS

The Pivotal Role of Vascularization in Tissue Engineering

(2013) François A. Auger et al.   Annual Review of Biomedical Engineering

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Pyramidal Neurons Derived from Human Pluripotent Stem Cells Integrate Efficiently into Mouse Brain Circuits In Vivo

(2013) Ira Espuny-Camacho et al.   NEURON

Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling

(2013) Lucy A. Crompton et al.   Stem Cell Research

Bioreactors to influence stem cell fate: Augmentation of mesenchymal stem cell signaling pathways via dynamic culture systems

(2012) Andrew B. Yeatts et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Tracking Stem Cells for Cellular Therapy in Stroke

(2012) Nathan C. Manley et al.   CURRENT PHARMACEUTICAL DESIGN

Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010

(2012) Rafael Lozano et al.   LANCET

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

(2012) Jordan S. Miller et al.   NATURE MATERIALS

Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses

(2012) Yichen Shi et al.   NATURE NEUROSCIENCE

Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks

(2012) Yichen Shi et al.   Nature Protocols

Direct Comparisons of the Morphology, Migration, Cell Adhesions, and Actin Cytoskeleton of Fibroblasts in Four Different Three-Dimensional Extracellular Matrices

(2010) Kirsi M. Hakkinen et al.   TISSUE ENGINEERING PART A

A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis

(2009) Emma East et al.   Journal of Tissue Engineering and Regenerative Medicine

Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling

(2009) Stuart M Chambers et al.   NATURE BIOTECHNOLOGY

Long-Term, Stable Differentiation of Human Embryonic Stem Cell-Derived Neural Precursors Grafted into the Adult Mammalian Neostriatum

(2009) Igor Nasonkin et al.   STEM CELLS

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

(2008) K. Okita et al.   SCIENCE