CRISPR/Cas Technology in Pig-to-Human Xenotransplantation Research

DNA repair protein RAD51 enhances the CRISPR/Cas9-mediated knock-in efficiency in brain neurons

(2020) Taiga Kurihara et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

OffScan: a universal and fast CRISPR off-target sites detection tool

(2020) Yingbo Cui et al.   BMC GENOMICS

Production of ULBP1-KO pigs with human CD55 expression using CRISPR technology

(2020) Agnieszka Nowak-Terpiłowska et al.   JOURNAL OF APPLIED ANIMAL RESEARCH

Generation of GGTA1-/-β2M-/-CIITA-/- Pigs Using CRISPR/Cas9 Technology to Alleviate Xenogeneic Immune Reactions

(2020) Rui Fu et al.   TRANSPLANTATION

CRISPR-Cas12a: Functional overview and applications

(2020) Bijoya Paul et al.   Biomedical Journal

CRISPR system: Discovery, development and off-target detection

(2020) Shengmiao Chen et al.   CELLULAR SIGNALLING

Livestock Gene Editing by One-step Embryo Manipulation

(2020) Sergio Navarro-Serna et al.   JOURNAL OF EQUINE VETERINARY SCIENCE

Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation

(2020) Natalia Ryczek et al.   Genes

Application of Genetically Engineered Pigs in Biomedical Research

(2020) Magdalena Hryhorowicz et al.   Genes

Ethical considerations in xenotransplantation: a review

(2020) Nezerith Cengiz et al.   Current Opinion in Organ Transplantation

Prime editing for functional repair in patient-derived disease models

(2020) Imre F. Schene et al.   Nature Communications

Extensive germline genome engineering in pigs

(2020) Yanan Yue et al.   Nature Biomedical Engineering

Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos

(2020) Michael V. Zuccaro et al.   CELL

Kidney xenotransplantation: Recent progress in preclinical research

(2020) Xiao-Hua Yu et al.   CLINICA CHIMICA ACTA

Engineering of CRISPR-Cas12b for human genome editing

(2019) Jonathan Strecker et al.   Nature Communications

Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9

(2019) Becky Xu Hua Fu et al.   Nature Microbiology

Long-Term Survival of Pig-to-Rhesus Macaque Renal Xenografts is Dependent on CD4 T cell Depletion

(2019) S C Kim et al.   AMERICAN JOURNAL OF TRANSPLANTATION

31 Day Xeno Lung Recipient Survival - Progress towards the Clinic

(2019) L. Burdorf et al.   JOURNAL OF HEART AND LUNG TRANSPLANTATION

Third WHO Global Consultation on Regulatory Requirements for Xenotransplantation Clinical Trials, Changsha, Hunan, China December 12–14, 2018

(2019) Wayne J. Hawthorne et al.   XENOTRANSPLANTATION

Evaluation and Reduction of CRISPR Off-Target Cleavage Events

(2019) Christopher A. Vakulskas et al.   Nucleic Acid Therapeutics

Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

(2019) Holly A. Rees et al.   Nature Communications

Dead Cas Systems: Types, Principles, and Applications

(2019) Sergey Brezgin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Characterization of porcine endogenous retrovirus particles released by the CRISPR/Cas9 inactivated cell line PK15 clone 15

(2019) Antonia W. Godehardt et al.   XENOTRANSPLANTATION

Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2

(2019) Konrad Fischer et al.   XENOTRANSPLANTATION

Generation of pRSAD2 gene knock-in pig via CRISPR/Cas9 technology

(2019) Zicong Xie et al.   ANTIVIRAL RESEARCH

Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines

(2019) Zheng-wei ZHOU et al.   Journal of Integrative Agriculture

Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH

(2018) Runjie Zhang et al.   Acta Biomaterialia

Coagulation factor XII in thrombosis and inflammation

(2018) Coen Maas et al.   BLOOD

Thrombus Formation and Propagation in the Onset of Cardiovascular Events

(2018) Yujiro Asada et al.   Journal of Atherosclerosis and Thrombosis

Tissue Factor at the crossroad of coagulation and cell signaling

(2018) Hortensia Zelaya et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Generation of GTKO Diannan Miniature Pig Expressing Human Complementary Regulator Proteins hCD55 and hCD59 via T2A Peptide-Based Bicistronic Vectors and SCNT

(2018) Fengjuan Liu et al.   MOLECULAR BIOTECHNOLOGY

CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9

(2018) Gaurav Dugar et al.   MOLECULAR CELL

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

Why was PERV not transmitted during preclinical and clinical xenotransplantation trials and after inoculation of animals?

(2018) Joachim Denner   Retrovirology

B4GALNT2 and xenotransplantation: A newly appreciated xenogeneic antigen

(2018) Guerard Byrne et al.   XENOTRANSPLANTATION

The production of UL16-binding protein 1 targeted pigs using CRISPR technology

(2018) Zeyland Joanna et al.   3 Biotech

The production of UL16-binding protein 1 targeted pigs using CRISPR technology

(2018) Zeyland Joanna et al.   3 Biotech

Quantitative assessment of HR and NHEJ activities via CRISPR/Cas9-induced oligodeoxynucleotide-mediated DSB repair

(2018) Jie Du et al.   DNA REPAIR

Porcine endogenous retroviruses: Quantification of the copy number in cell lines, pig breeds, and organs

(2018) Uwe Fiebig et al.   XENOTRANSPLANTATION

Xenoantigen Deletion and Chemical Immunosuppression Can Prolong Renal Xenograft Survival

(2018) Andrew B. Adams et al.   ANNALS OF SURGERY

Mosaicism in CRISPR/Cas9-mediated genome editing

(2018) Maryam Mehravar et al.   DEVELOPMENTAL BIOLOGY

Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles into Porcine Fibroblast

(2018) Magdalena Hryhorowicz et al.   MOLECULAR BIOTECHNOLOGY

Consistent success in life-supporting porcine cardiac xenotransplantation

(2018) Matthias Längin et al.   NATURE

Cardiac xenografts show reduced survival in the absence of transgenic human thrombomodulin expression in donor pigs

(2018) Avneesh K. Singh et al.   XENOTRANSPLANTATION

Minimal PAM specificity of a highly similar SpCas9 ortholog

(2018) Pranam Chatterjee et al.   Science Advances

Nonhomologous DNA end-joining for repair of DNA double-strand breaks

(2017) Nicholas R. Pannunzio et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning

(2017) Hanchao GAO et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Genetically Modified Pigs as Organ Donors for Xenotransplantation

(2017) Magdalena Hryhorowicz et al.   MOLECULAR BIOTECHNOLOGY

Non-homologous DNA end joining and alternative pathways to double-strand break repair

(2017) Howard H. Y. Chang et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

(2017) Dong Niu et al.   SCIENCE

Humoral Reactivity of Renal Transplant-Waitlisted Patients to Cells From GGTA1/CMAH/B4GalNT2, and SLA Class I Knockout Pigs

(2017) Gregory R. Martens et al.   TRANSPLANTATION

Eliminating Xenoantigen Expression on Swine RBC

(2017) Zheng-Yu Wang et al.   TRANSPLANTATION

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood

(2017) Gisella Puga Yung et al.   XENOTRANSPLANTATION

Targeted insertion of an anti-CD2 monoclonal antibody transgene into the GGTA1 locus in pigs using FokI-dCas9

(2017) Mark B. Nottle et al.   Scientific Reports

The CRISPR-Cas9 system in Neisseria spp.

(2017) Yan Zhang   Pathogens and Disease

The Role of Costimulation Blockade in Solid Organ and Islet Xenotransplantation

(2017) Kannan P. Samy et al.   Journal of Immunology Research

Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model

(2017) Junfang Zhang et al.   Scientific Reports

Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

(2016) Chin-Kai Chuang et al.   ANIMAL BIOTECHNOLOGY

Transplantology: Challenges for Today

(2016) Maria Boratyńska et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

DNA polymerase θ (POLQ), double-strand break repair, and cancer

(2016) Richard D. Wood et al.   DNA REPAIR

The fate of human platelets exposed to porcine renal endothelium: a single-pass model of platelet uptake in domestic and genetically modified porcine organs

(2016) James R. Butler et al.   JOURNAL OF SURGICAL RESEARCH

Contact system revisited: an interface between inflammation, coagulation, and innate immunity

(2016) A. T. Long et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Immunological methods for the detection of porcine lymphotropic herpesviruses (PLHV)

(2016) Elena Plotzki et al.   JOURNAL OF VIROLOGICAL METHODS

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

(2016) Maximilian Müller et al.   MOLECULAR THERAPY

Silencing porcine genes significantly reduces human-anti-pig cytotoxicity profiles: an alternative to direct complement regulation

(2016) James R. Butler et al.   TRANSGENIC RESEARCH

Silencing Porcine CMAH and GGTA1 Genes Significantly Reduces Xenogeneic Consumption of Human Platelets by Porcine Livers

(2016) James Russell Butler et al.   TRANSPLANTATION

Silencing the porcine iGb3s gene does not affect Galα3Gal levels or measures of anticipated pig-to-human and pig-to-primate acute rejection

(2016) James R. Butler et al.   XENOTRANSPLANTATION

Efficient production of biallelicGGTA1knockout pigsbycytoplasmic microinjection of CRISPR/Cas9 into zygotes

(2016) Bjoern Petersen et al.   XENOTRANSPLANTATION

Extended microbiological characterization of Göttingen minipigs: porcine cytomegalovirus and other viruses

(2016) Vladimir A. Morozov et al.   XENOTRANSPLANTATION

Chimeric 2C10R4 anti-CD40 antibody therapy is critical for long-term survival of GTKO.hCD46.hTBM pig-to-primate cardiac xenograft

(2016) Muhammad M. Mohiuddin et al.   Nature Communications

Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing

(2016) Konrad Fischer et al.   Scientific Reports

CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

(2016) Ting-Wei Will Chiang et al.   Scientific Reports

Efficient generation of B2m-null pigs via injection of zygote with TALENs

(2016) Yong Wang et al.   Scientific Reports

Recent advances in understanding xenotransplantation: implications for the clinic

(2015) David KC Cooper et al.   Expert Review of Clinical Immunology

The role of genetically engineered pigs in xenotransplantation research

(2015) David KC Cooper et al.   JOURNAL OF PATHOLOGY

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

(2015) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

(2015) L. Yang et al.   SCIENCE

The immunological barriers to xenotransplantation

(2015) M. Vadori et al.   TISSUE ANTIGENS

The Role of Natural Killer Cells in Humoral Rejection

(2015) Thomas Resch et al.   TRANSPLANTATION

Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes

(2015) Jose L. Estrada et al.   XENOTRANSPLANTATION

Immunobiological barriers to xenotransplantation

(2015) David K.C. Cooper et al.   International Journal of Surgery

A brief history of clinical xenotransplantation

(2015) David K.C. Cooper et al.   International Journal of Surgery

Creating Class I MHC–Null Pigs Using Guide RNA and the Cas9 Endonuclease

(2014) Luz M. Reyes et al.   JOURNAL OF IMMUNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage

(2014) Regina Cencic et al.   PLoS One

Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

(2014) L. Davis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection

(2014) Ping Li et al.   XENOTRANSPLANTATION

hCTLA4-Ig transgene expression in keratocytes modulates rejection of corneal xenografts in a pig to non-human primate anterior lamellar keratoplasty model

(2014) Bertrand Vabres et al.   XENOTRANSPLANTATION

Erythrocytes from GGTA1/CMAH knockout pigs: implications for xenotransfusion and testing in non-human primates

(2014) Zheng-Yu Wang et al.   XENOTRANSPLANTATION

Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen

(2014) Guerard W. Byrne et al.   XENOTRANSPLANTATION

Modernizing the Nonhomologous End-Joining Repertoire: Alternative and Classical NHEJ Share the Stage

(2013) Ludovic Deriano et al.   Annual Review of Genetics

Controlling Natural Killer Cell Responses: Integration of Signals for Activation and Inhibition

(2013) Eric O. Long et al.   Annual Review of Immunology

Human dominant-negative class II transactivator transgenic pigs - effect on the human anti-pig T-cell immune response and immune status

(2013) Hidetaka Hara et al.   IMMUNOLOGY

Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts

(2013) L. Scobie et al.   JOURNAL OF IMMUNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

(2013) Tautvydas Karvelis et al.   RNA Biology

The Potential Role of Genetically-Modified Pig Mesenchymal Stromal Cells in Xenotransplantation

(2013) Jiang Li et al.   Stem Cell Reviews and Reports

New Concepts of Immune Modulation in Xenotransplantation

(2013) Vikas Satyananda et al.   TRANSPLANTATION

Hyperacute rejection in the xenogenic transplanted rat liver is triggered by the complement system only in the presence of leukocytes and free radical species

(2013) Ba Thanh-Truc Ngo et al.   XENOTRANSPLANTATION

Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition

(2013) Hayato Iwase et al.   XENOTRANSPLANTATION

Transgenic swine: Expression of human CD39 protects against myocardial injury

(2012) Debra G. Wheeler et al.   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

ASGR1 expressed by porcine enriched liver sinusoidal endothelial cells mediates human platelet phagocytosis in vitro

(2011) Leela L. Paris et al.   XENOTRANSPLANTATION

Coagulation, platelet activation and thrombosis in xenotransplantation

(2010) Moritz Schmelzle et al.   Current Opinion in Organ Transplantation

Embryonic and adult stem cell therapy

(2010) Anne C. Brignier et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Atorvastatin or transgenic expression of TFPI inhibits coagulation initiated by anti-nonGal IgG binding to porcine aortic endothelial cells

(2010) C. C. LIN et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Alternative end-joining is suppressed by the canonical NHEJ component Xrcc4–ligase IV during chromosomal translocation formation

(2010) Deniz Simsek et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Potential value of human thrombomodulin and DAF expression for coagulation control in pig-to-human xenotransplantation

(2010) Yuko Miwa et al.   XENOTRANSPLANTATION

Deficiency of N-glycolylneuraminic acid and Galα1-3Galβ1-4GlcNAc epitopes in xenogeneic cells attenuates cytotoxicity of human natural antibodies

(2010) Nabin B. Basnet et al.   XENOTRANSPLANTATION

Development of a Severe von Willebrand Factor/ADAMTS13 Dysbalance During Orthotopic Liver Transplantation

(2009) I. T. A. Pereboom et al.   AMERICAN JOURNAL OF TRANSPLANTATION

Complement activation and coagulation in xenotransplantation

(2009) Peter J Cowan et al.   IMMUNOLOGY AND CELL BIOLOGY

Production and characterization of transgenic pigs expressing porcine CTLA4-Ig

(2009) Carol J. Phelps et al.   XENOTRANSPLANTATION

Current status of xenotransplantation and prospects for clinical application

(2009) Richard N. Pierson et al.   XENOTRANSPLANTATION

Pig Thrombomodulin Binds Human Thrombin but Is a Poor Cofactor for Activation of Human Protein C and TAFI

(2008) J. C. Roussel et al.   AMERICAN JOURNAL OF TRANSPLANTATION

Mechanisms of Thrombus Formation

(2008) Bruce Furie et al.   NEW ENGLAND JOURNAL OF MEDICINE

Coagulation dysregulation as a barrier to xenotransplantation in the primate

(2008) Chih Che Lin et al.   TRANSPLANT IMMUNOLOGY

The Galα1,3Galβ1,4GlcNAc-R (α-Gal) epitope: A carbohydrate of unique evolution and clinical relevance

(2007) Bruce A. Macher et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS