Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation

Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9

(2019) Becky Xu Hua Fu et al.   Nature Microbiology

Highly Efficient and Marker-free Genome Editing of Human Pluripotent Stem Cells by CRISPR-Cas9 RNP and AAV6 Donor-Mediated Homologous Recombination

(2019) Renata M. Martin et al.   Cell Stem Cell

Evaluation and Reduction of CRISPR Off-Target Cleavage Events

(2019) Christopher A. Vakulskas et al.   Nucleic Acid Therapeutics

Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production

(2019) Hui Yang et al.   BIOTECHNOLOGY LETTERS

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing

(2019) Pin Lyu et al.   NUCLEIC ACIDS RESEARCH

CRISPR/Cas9-Mediated Knock-Out of KrasG12D Mutated Pancreatic Cancer Cell Lines

(2019) Eva Lentsch et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2

(2019) Konrad Fischer et al.   XENOTRANSPLANTATION

OUP accepted manuscript

(2018)   BIOINFORMATICS

Bringing Home The Bacon: Update on The State of Kidney Xenotransplantation

(2018) David K.C. Cooper et al.   BLOOD PURIFICATION

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

B4GALNT2 and xenotransplantation: A newly appreciated xenogeneic antigen

(2018) Guerard Byrne et al.   XENOTRANSPLANTATION

A Survey of Validation Strategies for CRISPR-Cas9 Editing

(2018) Monica F. Sentmanat et al.   Scientific Reports

Review of CRISPR/Cas9 sgRNA Design Tools

(2018) Yingbo Cui et al.   Interdisciplinary Sciences-Computational Life Sciences

Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

(2018) Benjamin Steyer et al.   Stem Cell Reports

Quantitative assessment of HR and NHEJ activities via CRISPR/Cas9-induced oligodeoxynucleotide-mediated DSB repair

(2018) Jie Du et al.   DNA REPAIR

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

(2017) Shengdar Q Tsai et al.   NATURE METHODS

Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence

(2017) Ting Zheng et al.   Scientific Reports

Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

(2016) Chin-Kai Chuang et al.   ANIMAL BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

The pathobiology of pig-to-primate xenotransplantation: a historical review

(2016) David K.C. Cooper et al.   XENOTRANSPLANTATION

“Off-Spotter”: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs

(2015) Venetia Pliatsika et al.   Biology Direct

Recent advances in understanding xenotransplantation: implications for the clinic

(2015) David KC Cooper et al.   Expert Review of Clinical Immunology

Fast and sensitive detection of indels induced by precise gene targeting

(2015) Zhang Yang et al.   NUCLEIC ACIDS RESEARCH

The immunological barriers to xenotransplantation

(2015) M. Vadori et al.   TISSUE ANTIGENS

Reduced human platelet uptake by pig livers deficient in the asialoglycoprotein receptor 1 protein

(2015) Leela L. Paris et al.   XENOTRANSPLANTATION

Immunobiological barriers to xenotransplantation

(2015) David K.C. Cooper et al.   International Journal of Surgery

A brief history of clinical xenotransplantation

(2015) David K.C. Cooper et al.   International Journal of Surgery

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

CRISPRseek: A Bioconductor Package to Identify Target-Specific Guide RNAs for CRISPR-Cas9 Genome-Editing Systems

(2014) Lihua J. Zhu et al.   PLoS One

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection

(2014) Ping Li et al.   XENOTRANSPLANTATION

Erythrocytes from GGTA1/CMAH knockout pigs: implications for xenotransfusion and testing in non-human primates

(2014) Zheng-Yu Wang et al.   XENOTRANSPLANTATION

Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen

(2014) Guerard W. Byrne et al.   XENOTRANSPLANTATION

Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts

(2013) L. Scobie et al.   JOURNAL OF IMMUNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Coagulation dysregulation as a barrier to xenotransplantation in the primate

(2008) Chih Che Lin et al.   TRANSPLANT IMMUNOLOGY