The Ku complex: recent advances and emerging roles outside of non-homologous end-joining

The roles of RNA in DNA double-strand break repair

(2020) Aldo S. Bader et al.   BRITISH JOURNAL OF CANCER

DNA–dependent protein kinase in telomere maintenance and protection

(2020) Jiangdong Sui et al.   CELLULAR & MOLECULAR BIOLOGY LETTERS

DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis

(2020) Zhengping Shao et al.   NATURE

DNA-dependent protein kinase promotes DNA end processing by MRN and CtIP

(2020) Rajashree A. Deshpande et al.   Science Advances

Analysis of RNA binding properties of human Ku protein reveals its interactions with 7SK snRNA and protein components of 7SK snRNP complex

(2020) Olga Shadrina et al.   BIOCHIMIE

Nocodazole-induced expression and phosphorylation of anillin and other mitotic proteins is decreased in DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-deficient cells and rescued by inhibition of the Anaphase Promoting Complex/Cyclosome (APC/C) with proTAME but not apcin.

(2020) Pauline Douglas et al.   MOLECULAR AND CELLULAR BIOLOGY

Dimers of DNA-PK create a stage for DNA double-strand break repair

(2020) Amanda K. Chaplin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanism of efficient double-strand break repair by a long non-coding RNA

(2020) Roopa Thapar et al.   NUCLEIC ACIDS RESEARCH

Uncovering DNA-PKcs ancient phylogeny, unique sequence motifs and insights for human disease

(2020) James P. Lees-Miller et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Plugged into the Ku-DNA hub: The NHEJ network

(2019) Philippe Frit et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Telomeres and telomerase: three decades of progress

(2019) Jerry W. Shay et al.   NATURE REVIEWS GENETICS

R-Loops as Cellular Regulators and Genomic Threats

(2019) Madzia P. Crossley et al.   MOLECULAR CELL

Anti-Ku syndrome with elevated CK and anti-Ku syndrome with anti-dsDNA are two distinct entities with different outcomes

(2019) Lionel Spielmann et al.   ANNALS OF THE RHEUMATIC DISEASES

DNA double-strand break repair-pathway choice in somatic mammalian cells

(2019) Ralph Scully et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes

(2019) Anna Nenarokova et al.   mBio

Ku80 gene knockdown by the CRISPR/Cas9 technique affects the biological functions of human thyroid carcinoma cells

(2019) Yali Fan et al.   ONCOLOGY REPORTS

Overlap of systemic lupus erythematosus and myositis is rare in anti-Ku antibody-positive patients

(2019) Mariko Ogawa-Momohara et al.   ANNALS OF THE RHEUMATIC DISEASES

Ligand binding characteristics of the Ku80 von Willebrand domain

(2019) Kyungmin Kim et al.   DNA REPAIR

Structural Insights into Yeast Telomerase Recruitment to Telomeres

(2018) Hongwen Chen et al.   CELL

Telomeres and aging

(2018) Jerry W Shay   CURRENT OPINION IN CELL BIOLOGY

The role of DNA-PK in aging and energy metabolism

(2018) Jay H. Chung   FEBS Journal

MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining

(2018) Putzer J. Hung et al.   MOLECULAR CELL

Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair

(2018) Wei-Ting Lu et al.   Nature Communications

RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage

(2018) Agnese Cristini et al.   Cell Reports

Targeting RNA in mammalian systems with small molecules

(2018) Anita Donlic et al.   Wiley Interdisciplinary Reviews-RNA

Human proteins that interact with RNA/DNA hybrids

(2018) Isabel X. Wang et al.   GENOME RESEARCH

XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining

(2018) Clement Nemoz et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mapping the Ku Interactome Using Proximity-Dependent Biotin Identification in Human Cells

(2018) Sanna Abbasi et al.   JOURNAL OF PROTEOME RESEARCH

Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligase

(2018) Sudipta Bhattacharyya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA-PK Is Targeted by Multiple Vaccinia Virus Proteins to Inhibit DNA Sensing

(2018) Simon R. Scutts et al.   Cell Reports

Ku70 Senses HTLV-1 DNA and Modulates HTLV-1 Replication

(2017) Jie Wang et al.   JOURNAL OF IMMUNOLOGY

DNA-Damage Response RNA-Binding Proteins (DDRBPs): Perspectives from a New Class of Proteins and Their RNA Targets

(2017) Martin Dutertre et al.   JOURNAL OF MOLECULAR BIOLOGY

ATM, ATR, and DNA-PK: The Trinity at the Heart of the DNA Damage Response

(2017) Andrew N. Blackford et al.   MOLECULAR CELL

Cryo-EM structure of the DNA-PK holoenzyme

(2017) Humayun Sharif et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA-PKcs structure suggests an allosteric mechanism modulating DNA double-strand break repair

(2017) Bancinyane L. Sibanda et al.   SCIENCE

STING is an essential mediator of the Ku70-mediated production of IFN-λ1 in response to exogenous DNA

(2017) Hongyan Sui et al.   Science Signaling

The end-joining factor Ku acts in the end-resection of double strand break-free arrested replication forks

(2017) Ana Teixeira-Silva et al.   Nature Communications

STING is an essential mediator of the Ku70-mediated production of IFN-λ1 in response to exogenous DNA

(2017) Hongyan Sui et al.   Science Signaling

Cloning of canine Ku80 and its localization and accumulation at DNA damage sites

(2017) Manabu Koike et al.   FEBS Open Bio

The Role of the Core Non-Homologous End Joining Factors in Carcinogenesis and Cancer

(2017) et al.   Cancers

Consider the workhorse: Nonhomologous end-joining in budding yeast

(2016) Charlene H. Emerson et al.   Biochemistry and Cell Biology

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

(2016) Corina Ohle et al.   CELL

End-processing nucleases and phosphodiesterases: An elite supporting cast for the non-homologous end joining pathway of DNA double-strand break repair

(2016) Vijay Menon et al.   DNA REPAIR

A novel small molecule inhibitor of the DNA repair protein Ku70/80

(2016) Eric Weterings et al.   DNA REPAIR

A novel cytoprotective function for the DNA repair protein Ku in regulating p53 mRNA translation and function

(2016) Assala Lamaa et al.   EMBO REPORTS

Single-specificity anti-Ku antibodies in an international cohort of 2140 systemic sclerosis subjects

(2016) S. Hoa et al.   MEDICINE

Microhomology-mediated end joining is the principal mediator of double-strand break repair during mitochondrial DNA lesions

(2016) Satish Kumar Tadi et al.   MOLECULAR BIOLOGY OF THE CELL

Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA

(2016) Ineke Brouwer et al.   NATURE

Non-coding RNAs as drug targets

(2016) Masayuki Matsui et al.   NATURE REVIEWS DRUG DISCOVERY

Regulation of DNA double-strand break repair by ubiquitin and ubiquitin-like modifiers

(2016) Petra Schwertman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer

(2016) Youyou Zhang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phosphorylation of Ku70 subunit by cell cycle kinases modulates the replication related function of Ku heterodimer

(2016) Soumita Mukherjee et al.   NUCLEIC ACIDS RESEARCH

EAF2 regulates DNA repair through Ku70/Ku80 in the prostate

(2016) J Ai et al.   ONCOGENE

The Ku-binding motif is a conserved module for recruitment and stimulation of non-homologous end-joining proteins

(2016) Gabrielle J. Grundy et al.   Nature Communications

Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer

(2016) Youyou Zhang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ku70 Serine 155 mediates Aurora B inhibition and activation of the DNA damage response

(2016) Victoria L. Fell et al.   Scientific Reports

PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF

(2016) Satish K. Tadi et al.   Cell Reports

Cytoplasm-Translocated Ku70/80 Complex Sensing of HBV DNA Induces Hepatitis-Associated Chemokine Secretion

(2016) Young Li et al.   Frontiers in Immunology

A cross-sectional study of autoantibody profiles in the Waikato systemic sclerosis cohort, New Zealand

(2015) Winston S. J. Chang et al.   CLINICAL RHEUMATOLOGY

Cross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification

(2015) Matthew M. Makowski et al.   MOLECULAR & CELLULAR PROTEOMICS

Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86

(2015) Kevin W. Trotter et al.   MOLECULAR AND CELLULAR BIOLOGY

The Ku heterodimer: Function in DNA repair and beyond

(2015) Victoria L. Fell et al.   MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH

R loops: new modulators of genome dynamics and function

(2015) José M. Santos-Pereira et al.   NATURE REVIEWS GENETICS

Fungal Ku prevents permanent cell cycle arrest by suppressing DNA damage signaling at telomeres

(2015) Carmen de Sena-Tomás et al.   NUCLEIC ACIDS RESEARCH

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase

(2015) Kyung-Jong Lee et al.   NUCLEIC ACIDS RESEARCH

SIRT1 downregulation enhances chemosensitivity and survival of adult T-cell leukemia-lymphoma cells by reducing DNA double-strand repair

(2015) LIANG LI et al.   ONCOLOGY REPORTS

The DNA-dependent protein kinase: A multifunctional protein kinase with roles in DNA double strand break repair and mitosis

(2015) Nicholas Jette et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Gene essentiality and synthetic lethality in haploid human cells

(2015) V. A. Blomen et al.   SCIENCE

Transcriptional elongation requires DNA break-induced signalling

(2015) Heeyoun Bunch et al.   Nature Communications

A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines

(2015) Julien Bouley et al.   Oncotarget

Ku80 cooperates with CBP to promote COX-2 expression and tumor growth

(2015) Yao Xiao et al.   Oncotarget

Neddylation Promotes Ubiquitylation and Release of Ku from DNA-Damage Sites

(2015) Jessica S. Brown et al.   Cell Reports

A beginning of the end: new insights into the functional organization of telomeres

(2015) Ashley M Wood et al.   Nucleus

Interpretation of an Extended Autoantibody Profile in a Well-Characterized Australian Systemic Sclerosis (Scleroderma) Cohort Using Principal Components Analysis

(2015) K. A. Patterson et al.   Arthritis & Rheumatology

A beginning of the end: new insights into the functional organization of telomeres

(2015) Ashley M Wood et al.   Nucleus

Ku Stabilizes Replication Forks in the Absence of Brc1

(2015) Arancha Sánchez et al.   PLoS One

Loss of expression of the double strand break repair protein ATM is associated with worse prognosis in colorectal cancer and loss of Ku70 expression is associated with CIN

(2015) Andrew D Beggs et al.   Oncotarget

RNA binding protein RBM14 promotes radio-resistance in glioblastoma by regulating DNA repair and cell differentiation

(2015) Ming Yuan et al.   Oncotarget

Deacetylation of Ku70 regulates ionizing-radiation induced DNA damage responses in human cells

(2014) Ahmed Al-Emam et al.   BMC GENOMICS

Non-homologous end joining: Emerging themes and unanswered questions

(2014) Sarvan Kumar Radhakrishnan et al.   DNA REPAIR

The clinical impact of deficiency in DNA non-homologous end-joining

(2014) Lisa Woodbine et al.   DNA REPAIR

Regulation of Ku-DNA Association by Yku70 C-terminal Tail and SUMO Modification

(2014) Lisa E. Hang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal

(2014) Sébastien Britton et al.   NUCLEIC ACIDS RESEARCH

SMAR1 coordinates HDAC6-induced deacetylation of Ku70 and dictates cell fate upon irradiation

(2014) N Chaudhary et al.   Cell Death & Disease

CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori

(2014) Sanyuan Ma et al.   Scientific Reports

Modernizing the Nonhomologous End-Joining Repertoire: Alternative and Classical NHEJ Share the Stage

(2013) Ludovic Deriano et al.   Annual Review of Genetics

Inhibition of Ku70 acetylation by INHAT subunit SET/TAF-Iβ regulates Ku70-mediated DNA damage response

(2013) Kee-Beom Kim et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Pseudogenes as regulators of biological function

(2013) Ryan C. Pink et al.   Essays in Biochemistry

The N-terminal Region of the DNA-dependent Protein Kinase Catalytic Subunit Is Required for Its DNA Double-stranded Break-mediated Activation

(2013) Anthony J. Davis et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identification and Functional Characterization of a Ku-binding Motif in Aprataxin Polynucleotide Kinase/Phosphatase-like Factor (APLF)

(2013) Purnata Shirodkar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Telomeres and age-related disease: how telomere biology informs clinical paradigms

(2013) Mary Armanios   JOURNAL OF CLINICAL INVESTIGATION

Impact of the Ku Complex on HIV-1 Expression and Latency

(2013) Gwenola Manic et al.   PLoS One

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates

(2013) E. J. Bartlett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Mechanism for the Inhibition of DNA-PK-Mediated DNA Sensing by a Virus

(2013) Nicholas E. Peters et al.   PLoS Pathogens

Ku Protein Levels, Localization and Association to Replication Origins in Different Stages of Breast Tumor Progression

(2013) Khalil Abdelbaqi et al.   Journal of Cancer

Mutually Exclusive Binding of Telomerase RNA and DNA by Ku Alters Telomerase Recruitment Model

(2012) Jennifer S. Pfingsten et al.   CELL

Rapid Determination of Multiple Linear Kinase Substrate Motifs by Mass Spectrometry

(2012) Arminja N. Kettenbach et al.   CHEMISTRY & BIOLOGY

APLF promotes the assembly and activity of non-homologous end joining protein complexes

(2012) Gabrielle J Grundy et al.   EMBO JOURNAL

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

Ku80 is highly expressed in lung adenocarcinoma and promotes cisplatin resistance

(2012) Qingshan Ma et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

DNA Ends Alter the Molecular Composition and Localization of Ku Multicomponent Complexes

(2012) Guillaume Adelmant et al.   MOLECULAR & CELLULAR PROTEOMICS

CREB-Binding Protein Regulates Ku70 Acetylation in Response to Ionization Radiation in Neuroblastoma

(2012) C. Subramanian et al.   MOLECULAR CANCER RESEARCH

Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population

(2012) A. H. L. Low et al.   RHEUMATOLOGY

Large-Scale Screening for Targeted Knockouts in the Caenorhabditis elegans Genome

(2012)   G3-Genes Genomes Genetics

DNA-PK is a DNA sensor for IRF-3-dependent innate immunity

(2012) Brian J Ferguson et al.   eLife

Distinctive autoantibody profile in Mexican Mestizo systemic sclerosis patients

(2011) Tatiana S. Rodriguez-Reyna et al.   AUTOIMMUNITY

Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression

(2011) Sabina Pucci et al.   CELL CYCLE

KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair

(2011) Manabu Koike et al.   EXPERIMENTAL CELL RESEARCH

Destroying the ring: Freeing DNA from Ku with ubiquitin

(2011) Lisa Postow   FEBS LETTERS

Cutting Edge: Ku70 Is a Novel Cytosolic DNA Sensor That Induces Type III Rather Than Type I IFN

(2011) X. Zhang et al.   JOURNAL OF IMMUNOLOGY

Putative binding modes of Ku70-SAP domain with double strand DNA: a molecular modeling study

(2011) Shaowen Hu et al.   JOURNAL OF MOLECULAR MODELING

Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation

(2011) Henna M. Moore et al.   MOLECULAR & CELLULAR PROTEOMICS

Ku Regulates Signaling to DNA Damage Response Pathways through the Ku70 von Willebrand A Domain

(2011) V. L. Fell et al.   MOLECULAR AND CELLULAR BIOLOGY

Functional Interplay of the Mre11 Nuclease and Ku in the Response to Replication-Associated DNA Damage

(2011) S. S. Foster et al.   MOLECULAR AND CELLULAR BIOLOGY

Recapitulation of premature ageing with iPSCs from Hutchinson–Gilford progeria syndrome

(2011) Guang-Hui Liu et al.   NATURE

A phospho-proteomic screen identifies substrates of the checkpoint kinase Chk1

(2011) Melanie Blasius et al.   GENOME BIOLOGY

Anti-Ku antibodies: Clinical, genetic and diagnostic insights

(2010) C. Belizna et al.   AUTOIMMUNITY REVIEWS

DNA double-strand break repair in Caenorhabditis elegans

(2010) Bennie B. L. G. Lemmens et al.   CHROMOSOMA

A structural model for regulation of NHEJ by DNA-PKcs autophosphorylation

(2010) Tracey A. Dobbs et al.   DNA REPAIR

The MRN complex in double-strand break repair and telomere maintenance

(2010) Brandon J. Lamarche et al.   FEBS LETTERS

Adaptive immunity

(2010) Francisco A. Bonilla et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Expression of TRF1, TRF2, TIN2, TERT, KU70, and BRCA1 proteins is associated with telomere shortening and may contribute to multistage carcinogenesis of gastric cancer

(2010) Hua Hu et al.   JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

Hdm2 is a ubiquitin ligase of Ku70-Akt promotes cell survival by inhibiting Hdm2-dependent Ku70 destabilization

(2009) V Gama et al.   CELL DEATH AND DIFFERENTIATION

Efficient Gene Replacements inToxoplasma gondiiStrains Deficient for Nonhomologous End Joining

(2009) Barbara A. Fox et al.   EUKARYOTIC CELL

Fission yeast Ku protein is required for recovery from DNA replication stress

(2009) Tomoichiro Miyoshi et al.   GENES TO CELLS

Ku and DNA-dependent Protein Kinase Dynamic Conformations and Assembly Regulate DNA Binding and the Initial Non-homologous End Joining Complex

(2009) Michal Hammel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA-Dependent Protein Kinase (DNA-PK)-Dependent Cisplatin-Induced Loss of Nucleolar Facilitator of Chromatin Transcription (FACT) and Regulation of Cisplatin Sensitivity by DNA-PK and FACT

(2009) J. Dejmek et al.   MOLECULAR CANCER RESEARCH

The DNA-damage response in human biology and disease

(2009) Stephen P. Jackson et al.   NATURE

Ku's essential role in keeping telomeres intact

(2009) S. M. Indiviglio et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ku86 represses lethal telomere deletion events in human somatic cells

(2009) Y. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA-Dependent Conformational Changes in the Ku Heterodimer†

(2008) Jason A. Lehman et al.   BIOCHEMISTRY

Mutations to Ku reveal differences in human somatic cell lines

(2008) Kazi R. Fattah et al.   DNA REPAIR

TLC1 RNA nucleo-cytoplasmic trafficking links telomerase biogenesis to its recruitment to telomeres

(2008) Franck Gallardo et al.   EMBO JOURNAL

Ku80 removal from DNA through double strand break–induced ubiquitylation

(2008) Lisa Postow et al.   JOURNAL OF CELL BIOLOGY

Ku is involved in cell growth, DNA replication and G1-S transition

(2008) E. Rampakakis et al.   JOURNAL OF CELL SCIENCE

Prevalence and clinical associations of anti-Ku antibodies in systemic autoimmune diseases

(2008) I Cavazzana et al.   LUPUS

The Ku80 Carboxy Terminus Stimulates Joining and Artemis-Mediated Processing of DNA Ends

(2008) E. Weterings et al.   MOLECULAR AND CELLULAR BIOLOGY

Evidence for an inositol hexakisphosphate-dependent role for Ku in mammalian nonhomologous end joining that is independent of its role in the DNA-dependent protein kinase

(2008) Joyce C.Y. Cheung et al.   NUCLEIC ACIDS RESEARCH

Evidence for Ku70/Ku80 association with full-length RAG1

(2008) Prafulla Raval et al.   NUCLEIC ACIDS RESEARCH

Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells

(2008) F. J. Fattah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Solution structures and DNA binding properties of the N-terminal SAP domains of SUMO E3 ligases fromSaccharomyces cerevisiaeandOryza sativa

(2008) Rintaro Suzuki et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Identification of CDK2 substrates in human cell lysates

(2008) Yong Chi et al.   GENOME BIOLOGY

Accumulation of Ku80 proteins at DNA double-strand breaks in living cells

(2007) Manabu Koike et al.   EXPERIMENTAL CELL RESEARCH