Three-Dimensional Cell Cultures in Drug Discovery and Development

3D Bioprinting for Tissue and Organ Fabrication

(2016) Yu Shrike Zhang et al.   ANNALS OF BIOMEDICAL ENGINEERING

3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney

(2016) Yong Xu et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Testing chemotherapy efficacy in HER2 negative breast cancer using patient-derived spheroids

(2016) Kathrin Halfter et al.   Journal of Translational Medicine

High-throughput imaging: Focusing in on drug discovery in 3D

(2016) Linfeng Li et al.   METHODS

Analysis of Gene Expression in 3D Spheroids Highlights a Survival Role for ASS1 in Mesothelioma

(2016) Dario Barbone et al.   PLoS One

Bioprinting towards Physiologically Relevant Tissue Models for Pharmaceutics

(2016) Weijie Peng et al.   TRENDS IN BIOTECHNOLOGY

Spheroid Culture of Mesenchymal Stem Cells

(2016) Zoe Cesarz et al.   Stem Cells International

Matched-Comparative Modeling of Normal and Diseased Human Airway Responses Using a Microengineered Breathing Lung Chip

(2016) Kambez H. Benam et al.   Cell Systems

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Organoids and the genetically encoded self-assembly of embryonic stem cells

(2015) David A. Turner et al.   BIOESSAYS

Modeling mouse and human development using organoid cultures

(2015) M. Huch et al.   DEVELOPMENT

PTEN deletion potentiates invasion of colorectal cancer spheroidal cells through 3D Matrigel

(2015) Siddarth Chandrasekaran et al.   Integrative Biology

Three-Dimensional Cell Culture-Based Screening Identifies the Anthelmintic Drug Nitazoxanide as a Candidate for Treatment of Colorectal Cancer

(2015) W. Senkowski et al.   MOLECULAR CANCER THERAPEUTICS

The boom in mini stomachs, brains, breasts, kidneys and more

(2015) Cassandra Willyard   NATURE

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2015) Minoru Takasato et al.   NATURE

Organs-on-chips at the frontiers of drug discovery

(2015) Eric W. Esch et al.   NATURE REVIEWS DRUG DISCOVERY

Human pluripotent stem cell-derived neural constructs for predicting neural toxicity

(2015) Michael P. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Beyond 3D culture models of cancer

(2015) Kandice Tanner et al.   Science Translational Medicine

Compaction, Fusion, and Functional Activation of Three-Dimensional Human Mesenchymal Stem Cell Aggregate

(2015) Ang-Chen Tsai et al.   TISSUE ENGINEERING PART A

Quantitative high throughput screening using a primary human three-dimensional organotypic culture predicts in vivo efficacy

(2015) Hilary A. Kenny et al.   Nature Communications

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

(2015) Benjamin S. Freedman et al.   Nature Communications

Adult Lung Spheroid Cells Contain Progenitor Cells and Mediate Regeneration in Rodents With Bleomycin-Induced Pulmonary Fibrosis

(2015) Eric Henry et al.   Stem Cells Translational Medicine

Concise Review: Manufacturing of Pancreatic Endoderm Cells for Clinical Trials in Type 1 Diabetes

(2015) Thomas C. Schulz   Stem Cells Translational Medicine

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

Evidence for differential viral oncolytic efficacy in an in vitro model of epithelial ovarian cancer metastasis

(2015) Jessica G Tong et al.   Molecular Therapy-Oncolytics

Label-Free Single Cell Kinetics of the Invasion of Spheroidal Colon Cancer Cells through 3D Matrigel

(2014) Nicole K. Febles et al.   ANALYTICAL CHEMISTRY

Three-dimensional models of cancer for pharmacology and cancer cell biology: Capturing tumor complexity in vitro/ex vivo

(2014) John A. Hickman et al.   Biotechnology Journal

Lung Stem Cell Differentiation in Mice Directed by Endothelial Cells via a BMP4-NFATc1-Thrombospondin-1 Axis

(2014) Joo-Hyeon Lee et al.   CELL

Label-Free Cell Phenotypic Drug Discovery

(2014) Ye Fang   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions

(2014) Carsten Wenzel et al.   EXPERIMENTAL CELL RESEARCH

Clinical development success rates for investigational drugs

(2014) Michael Hay et al.   NATURE BIOTECHNOLOGY

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

3D bioprinting of tissues and organs

(2014) Sean V Murphy et al.   NATURE BIOTECHNOLOGY

An organized and functional thymus generated from FOXN1-reprogrammed fibroblasts

(2014) Nicholas Bredenkamp et al.   NATURE CELL BIOLOGY

Materials as stem cell regulators

(2014) William L. Murphy et al.   NATURE MATERIALS

Three-dimensional organotypic culture: experimental models of mammalian biology and disease

(2014) Eliah R. Shamir et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cell Invasion in the Spheroid Sprouting Assay: A Spatial Organisation Analysis Adaptable to Cell Behaviour

(2014) Silvia Blacher et al.   PLoS One

Expansion of Human Mesenchymal Stromal Cells from Fresh Bone Marrow in a 3D Scaffold-Based System under Direct Perfusion

(2014) Adam Papadimitropoulos et al.   PLoS One

Three-dimensionally printed biological machines powered by skeletal muscle

(2014) C. Cvetkovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability

(2013) Thomas Billiet et al.   BIOMATERIALS

Organ-on-a-chip technology and microfluidic whole-body models for pharmacokinetic drug toxicity screening

(2013) Jong Bum Lee et al.   Biotechnology Journal

Differentiated Troy+ Chief Cells Act as Reserve Stem Cells to Generate All Lineages of the Stomach Epithelium

(2013) Daniel E. Stange et al.   CELL

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

(2013) Robert P. Fordham et al.   Cell Stem Cell

Redefining the In Vivo Origin of Metanephric Nephron Progenitors Enables Generation of Complex Kidney Structures from Pluripotent Stem Cells

(2013) Atsuhiro Taguchi et al.   Cell Stem Cell

The mesenchymal stromal cells dilemma—does a negative phase III trial of random donor mesenchymal stromal cells in steroid-resistant graft-versus-host disease represent a death knell or a bump in the road?

(2013) Jacques Galipeau   CYTOTHERAPY

Artificial three-dimensional niches deconstruct pancreas development in vitro

(2013) C. Greggio et al.   DEVELOPMENT

Human Primary Intestinal Epithelial Cells as an ImprovedIn VitroModel for Cryptosporidium parvum Infection

(2013) Alejandro Castellanos-Gonzalez et al.   INFECTION AND IMMUNITY

TTC7A mutations disrupt intestinal epithelial apicobasal polarity

(2013) Amélie E. Bigorgne et al.   JOURNAL OF CLINICAL INVESTIGATION

Scaling and systems biology for integrating multiple organs-on-a-chip

(2013) John P. Wikswo et al.   LAB ON A CHIP

Accelerating drug discovery via organs-on-chips

(2013) Chung Yu Chan et al.   LAB ON A CHIP

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Determining target engagement in living systems

(2013) Gabriel M Simon et al.   Nature Chemical Biology

Metre-long cell-laden microfibres exhibit tissue morphologies and functions

(2013) Hiroaki Onoe et al.   NATURE MATERIALS

A functional CFTR assay using primary cystic fibrosis intestinal organoids

(2013) Johanna F Dekkers et al.   NATURE MEDICINE

Genetic reconstitution of tumorigenesis in primary intestinal cells

(2013) K. Onuma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size

(2013) Qiu Li Loh et al.   TISSUE ENGINEERING PART B-REVIEWS

3D Organotypic Cultures of Human HepaRG Cells: A Tool for In Vitro Toxicity Studies

(2013) Patrina Gunness et al.   TOXICOLOGICAL SCIENCES

Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

(2012) Maria Vinci et al.   BMC BIOLOGY

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Organotypic liver culture models: Meeting current challenges in toxicity testing

(2012) Edward L. LeCluyse et al.   CRITICAL REVIEWS IN TOXICOLOGY

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Loss of cancer drug activity in colon cancer HCT-116 cells during spheroid formation in a new 3-D spheroid cell culture system

(2012) Henning Karlsson et al.   EXPERIMENTAL CELL RESEARCH

Screening therapeutic EMT blocking agents in a three-dimensional microenvironment

(2012) Amir R. Aref et al.   Integrative Biology

Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy

(2012) Geeta Mehta et al.   JOURNAL OF CONTROLLED RELEASE

Characterization of drug permeability in Caco-2 monolayers by mass spectrometry on a membrane-based microfluidic device

(2012) Dan Gao et al.   LAB ON A CHIP

Generation of functional thyroid from embryonic stem cells

(2012) Francesco Antonica et al.   NATURE

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

(2012) Jordan S. Miller et al.   NATURE MATERIALS

Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell

(2012) Shiro Yui et al.   NATURE MEDICINE

Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture

(2012) Jiri Kalabis et al.   Nature Protocols

Printing and Prototyping of Tissues and Scaffolds

(2012) B. Derby   SCIENCE

Improving Risk Assessment

(2012) Donna M. Dambach et al.   Science Translational Medicine

Evaluation of seven drug metabolisms and clearances by cryopreserved human primary hepatocytes cultivated in microfluidic biochips

(2012) Régis Baudoin et al.   XENOBIOTICA

Stem Cell-Derived Human Intestinal Organoids as an Infection Model for Rotaviruses

(2012) S. R. Finkbeiner et al.   mBio

The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation

(2011) Yukie Yoshii et al.   BIOMATERIALS

Hallmarks of Cancer: The Next Generation

(2011) Douglas Hanahan et al.   CELL

Scaffold-free culture of mesenchymal stem cell spheroids in suspension preserves multilineage potential

(2011) Priya R. Baraniak et al.   CELL AND TISSUE RESEARCH

Distinct Types of Tumor-Initiating Cells Form Human Colon Cancer Tumors and Metastases

(2011) Sebastian M. Dieter et al.   Cell Stem Cell

Stem and progenitor cell-based therapy in ischaemic heart disease: promise, uncertainties, and challenges

(2011) Jörn Tongers et al.   EUROPEAN HEART JOURNAL

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

(2011) Sang Bok Kim et al.   LAB ON A CHIP

How were new medicines discovered?

(2011) David C. Swinney et al.   NATURE REVIEWS DRUG DISCOVERY

Trends in the exploitation of novel drug targets

(2011) Mathias Rask-Andersen et al.   NATURE REVIEWS DRUG DISCOVERY

The productivity crisis in pharmaceutical R&D

(2011) Fabio Pammolli et al.   NATURE REVIEWS DRUG DISCOVERY

From 3D cell culture to organs-on-chips

(2011) Dongeun Huh et al.   TRENDS IN CELL BIOLOGY

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Changes in the expression pattern of mesenchymal and pluripotent markers in human adipose-derived stem cells

(2010) Eulsoon Park et al.   CELL BIOLOGY INTERNATIONAL

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

(2010) Nick Barker et al.   Cell Stem Cell

Towards organ printing: engineering an intra-organ branched vascular tree

(2010) Richard P Visconti et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

A novel concept for scaffold-free vessel tissue engineering: Self-assembly of microtissue building blocks

(2010) Jens M. Kelm et al.   JOURNAL OF BIOTECHNOLOGY

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Cancer stem cells from colorectal cancer-derived cell lines

(2010) Trevor M. Yeung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Endothelial cell traction and ECM density influence both capillary morphogenesis and maintenance in 3-D

(2009) Ekaterina Kniazeva et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Evaluation of a microfluidic based cell culture platform with primary human hepatocytes for the prediction of hepatic clearance in human

(2009) P. Chao et al.   BIOCHEMICAL PHARMACOLOGY

Scaffold-free vascular tissue engineering using bioprinting

(2009) Cyrille Norotte et al.   BIOMATERIALS

Organ printing: Tissue spheroids as building blocks

(2009) Vladimir Mironov et al.   BIOMATERIALS

Hydrogels as extracellular matrix mimics for 3D cell culture

(2009) Mark W. Tibbitt et al.   BIOTECHNOLOGY AND BIOENGINEERING

Human adipose-derived mesenchymal stem cells in vitro: evaluation of an optimal expansion medium preserving stemness

(2009) Patrick C. Baer et al.   CYTOTHERAPY

Perfused multiwell plate for 3D liver tissue engineering

(2009) Karel Domansky et al.   LAB ON A CHIP

Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds

(2009) Yan Feng et al.   NATURE REVIEWS DRUG DISCOVERY

Microfluidic Endothelium for Studying the Intravascular Adhesion of Metastatic Breast Cancer Cells

(2009) Jonathan W. Song et al.   PLoS One

Physiological function and transplantation of scaffold-free and vascularized human cardiac muscle tissue

(2009) K. R. Stevens et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Human Breast Cell Model of Preinvasive to Invasive Transition

(2008) A. Rizki et al.   CANCER RESEARCH

Compact spheroid formation by ovarian cancer cells is associated with contractile behavior and an invasive phenotype

(2008) Katharine L. Sodek et al.   INTERNATIONAL JOURNAL OF CANCER

Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells

(2008) Danielle S. W. Benoit et al.   NATURE MATERIALS

Emergence of Patterned Stem Cell Differentiation Within Multicellular Structures

(2008) Sami Alom Ruiz et al.   STEM CELLS

Chondrogenic Differentiation of Human Mesenchymal Stem Cells on Oriented Nanofibrous Scaffolds: Engineering the Superficial Zone of Articular Cartilage

(2008) Joel K. Wise et al.   TISSUE ENGINEERING PART A