Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation

Insights into protein structure, stability and function from saturation mutagenesis

(2018) Kritika Gupta et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A natively paired antibody library yields drug leads with higher sensitivity and specificity than a randomly paired antibody library

(2018) Adam S. Adler et al.   mAbs

Antibodies to watch in 2018

(2018) Hélène Kaplon et al.   mAbs

The Increasingly Human and Profitable Monoclonal Antibody Market

(2018) António L. Grilo et al.   TRENDS IN BIOTECHNOLOGY

Landscape Phage: Evolution from Phage Display to Nanobiotechnology

(2018) Valery Petrenko   Viruses-Basel

Basics of Antibody Phage Display Technology

(2018) Line Ledsgaard et al.   Toxins

Repertoire Analysis of Antibody CDR-H3 Loops Suggests Affinity Maturation Does Not Typically Result in Rigidification

(2018) Jeliazko R. Jeliazkov et al.   Frontiers in Immunology

VDJServer: A Cloud-Based Analysis Portal and Data Commons for Immune Repertoire Sequences and Rearrangements

(2018) Scott Christley et al.   Frontiers in Immunology

Next-Generation Sequencing of Antibody Display Repertoires

(2018) Romain Rouet et al.   Frontiers in Immunology

Overview of Antibody Drug Delivery

(2018) Sahar Awwad et al.   Pharmaceutics

Cassette hybridization for vector assembly application in antibody chain shuffling

(2018) Jing Yi Lai et al.   BIOTECHNIQUES

Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen

(2017) Anizah Rahumatullah et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A high-throughput platform for population reformatting and mammalian expression of phage display libraries to enable functional screening as full-length IgG

(2017) Xiaodong Xiao et al.   mAbs

In silico methods for design of biological therapeutics

(2017) Ankit Roy et al.   METHODS

Designing Human Antibodies by Phage Display

(2017) André Frenzel et al.   TRANSFUSION MEDICINE AND HEMOTHERAPY

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

(2017) Randall A. Hughes et al.   Cold Spring Harbor Perspectives in Biology

Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis

(2017) Kathryn E. Tiller et al.   Frontiers in Immunology

Host-directed therapies for infectious diseases: current status, recent progress, and future prospects

(2016) Alimuddin Zumla et al.   LANCET INFECTIOUS DISEASES

Phage display-derived human antibodies in clinical development and therapy

(2016) André Frenzel et al.   mAbs

Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates

(2016) Kendrick B. Turner et al.   PLoS One

Generation of a naïve human single chain variable fragment (scFv) library for the identification of monoclonal scFv against Salmonella Typhi Hemolysin E antigen

(2016) Bee Nar Lim et al.   TOXICON

Genome-Editing Technologies: Principles and Applications

(2016) Thomas Gaj et al.   Cold Spring Harbor Perspectives in Biology

Isolation of Anti-Ricin Protective Antibodies Exhibiting High Affinity from Immunized Non-Human Primates

(2016) Tal Noy-Porat et al.   Toxins

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

(2016) Hasnain Hussain et al.   Biomed Research International

MDC-Analyzer: A novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites

(2016) Lixia Tang et al.   BIOTECHNIQUES

A truncated and dimeric format of an Affibody library on bacteria enables FACS-mediated isolation of amyloid-beta aggregation inhibitors with subnanomolar affinity

(2015) Hanna Lindberg et al.   Biotechnology Journal

Generation and analysis of the improved human HAL9/10 antibody phage display libraries

(2015) Jonas Kügler et al.   BMC BIOTECHNOLOGY

Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step

(2015) Renhua Huang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rapid Fine Conformational Epitope Mapping Using Comprehensive Mutagenesis and Deep Sequencing

(2015) Caitlin A. Kowalsky et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Design and construction of small perturbation mutagenesis libraries for antibody affinity maturation using massive microchip-synthesized oligonucleotides

(2015) Man Xu et al.   JOURNAL OF BIOTECHNOLOGY

Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library

(2015) Anizah Rahumatullah et al.   MOLECULAR IMMUNOLOGY

Massively parallel single-amino-acid mutagenesis

(2015) Jacob O Kitzman et al.   NATURE METHODS

Oligonucleotide-directed mutagenesis for precision gene editing

(2015) Noel J. Sauer et al.   PLANT BIOTECHNOLOGY JOURNAL

Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

(2015) Dongmei Hu et al.   PLoS One

Overlapping hotspots in CDRs are critical sites for V region diversification

(2015) Lirong Wei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A fully human scFv phage display library for rapid antibody fragment reformatting

(2015) Keyu Li et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Error-prone pcr-based mutagenesis strategy for rapidly generating high-yield influenza vaccine candidates

(2015) Jianqiang Ye et al.   VIROLOGY

Economical analysis of saturation mutagenesis experiments

(2015) Carlos G. Acevedo-Rocha et al.   Scientific Reports

Longitudinal analysis of the peripheral B cell repertoire reveals unique effects of immunization with a new influenza virus strain

(2015) Bernardo Cortina-Ceballos et al.   Genome Medicine

A truncated and dimeric format of an Affibody library on bacteria enables FACS-mediated isolation of amyloid-beta aggregation inhibitors with subnanomolar affinity

(2015) Hanna Lindberg et al.   Biotechnology Journal

VDJSeq-Solver: In Silico V(D)J Recombination Detection Tool

(2015) Giulia Paciello et al.   PLoS One

Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy

(2014) Aizhi Zhao et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Nucleotide Insertions and Deletions Complement Point Mutations to Massively Expand the Diversity Created by Somatic Hypermutation of Antibodies

(2014) Peter M. Bowers et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Antibody Phage Display: Technique and Applications

(2014) Christoph M. Hammers et al.   JOURNAL OF INVESTIGATIVE DERMATOLOGY

Restricted Diversity of Antigen Binding Residues of Antibodies Revealed by Computational Alanine Scanning of 227 Antibody–Antigen Complexes

(2014) Gautier Robin et al.   JOURNAL OF MOLECULAR BIOLOGY

Mining human antibody repertoires

(2014) Roger R. Beerli et al.   mAbs

The therapeutic monoclonal antibody market

(2014) Dawn M Ecker et al.   mAbs

Affinity maturation of a novel antagonistic human monoclonal antibody with a long VHCDR3 targeting the Class A GPCR formyl-peptide receptor 1

(2014) Julie A Douthwaite et al.   mAbs

Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays

(2014) Björn Forsström et al.   MOLECULAR & CELLULAR PROTEOMICS

The promise and challenge of high-throughput sequencing of the antibody repertoire

(2014) George Georgiou et al.   NATURE BIOTECHNOLOGY

Large-scale de novo DNA synthesis: technologies and applications

(2014) Sriram Kosuri et al.   NATURE METHODS

A Highly Functional Synthetic Phage Display Library Containing over 40 Billion Human Antibody Clones

(2014) Marcel Weber et al.   PLoS One

Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection

(2014) K. Halemano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ProxiMAX randomization: a new technology for non-degenerate saturation mutagenesis of contiguous codons

(2013) Mohammed Ashraf et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Directed evolution of nucleotide-based libraries using lambda exonuclease

(2013) Bee Nar Lim et al.   BIOTECHNIQUES

Polishing the craft of genetic diversity creation in directed evolution

(2013) Kang Lan Tee et al.   BIOTECHNOLOGY ADVANCES

To get what we aim for - progress in diversity generation methods

(2013) Anna J. Ruff et al.   FEBS Journal

Stability engineering of the human antibody repertoire

(2013) Romain Rouet et al.   FEBS LETTERS

Regulation of AID, the B-cell genome mutator

(2013) C. Keim et al.   GENES & DEVELOPMENT

Discovery of diverse and functional antibodies from large human repertoire antibody libraries

(2013) Lauren J. Schwimmer et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The influence of antibody fragment format on phage display based affinity maturation of IgG

(2013) Miriam Steinwand et al.   mAbs

A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties

(2013) Thomas Tiller et al.   mAbs

AAscan, PCRdesign and MutantChecker: A Suite of Programs for Primer Design and Sequence Analysis for High-Throughput Scanning Mutagenesis

(2013) Dawei Sun et al.   PLoS One

Cancer Immunotherapy

(2013) J. Couzin-Frankel   SCIENCE

The Application of Next Generation Sequencing to the Understanding of Antibody Repertoires

(2013) Pascale Mathonet et al.   Frontiers in Immunology

scFv Antibody: Principles and Clinical Application

(2012) Zuhaida Asra Ahmad et al.   Clinical & Developmental Immunology

Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens

(2012) Nicola Clementi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Antibody Phage Display Libraries: Contributions to Oncology

(2012) Carmela Dantas-Barbosa et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Phagemid Vectors for Phage Display: Properties, Characteristics and Construction

(2012) Huan Qi et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular engineering of antibodies for therapeutic and diagnostic purposes

(2012) Frédéric Ducancel et al.   mAbs

Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries

(2012) Renhua Huang et al.   METHODS

‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules

(2012) Hwangbeom Kim et al.   NUCLEIC ACIDS RESEARCH

Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification

(2012) Tuomas Huovinen et al.   PLoS One

High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

(2012) Audrey D. McConnell et al.   PLoS One

PFunkel: Efficient, Expansive, User-Defined Mutagenesis

(2012) Elad Firnberg et al.   PLoS One

Construction of a rationally designed antibody platform for sequencing-assisted selection

(2012) H. B. Larman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

(2012) Sabrina Kille et al.   ACS Synthetic Biology

Monoclonal antibodies: magic bullets with a hefty price tag

(2012) A. F. Shaughnessy   BMJ-British Medical Journal

Phage display—A powerful technique for immunotherapy

(2012) Justyna Bazan et al.   Human Vaccines & Immunotherapeutics

Phage display screening without repetitious selection rounds

(2011) Peter A.C. ’t Hoen et al.   ANALYTICAL BIOCHEMISTRY

When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis

(2011) Yuval Nov   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

In vitro methods for peptide display and their applications

(2011) C. G. Ullman et al.   Briefings in Functional Genomics

Identification of Candidate IgG Biomarkers for Alzheimer's Disease via Combinatorial Library Screening

(2011) M. Muralidhar Reddy et al.   CELL

Molecular considerations for development of phage antibody libraries

(2011) Mohammad Reza Tohidkia et al.   JOURNAL OF DRUG TARGETING

HuCAL PLATINUM, a Synthetic Fab Library Optimized for Sequence Diversity and Superior Performance in Mammalian Expression Systems

(2011) Josef Prassler et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel synthetic naïve human antibody library allows the isolation of antibodies against a new epitope of oncofetal fibronectin

(2011) Alessandra Villa et al.   mAbs

DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods

(2011) Thomas Lindner et al.   MOLECULES

High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation

(2011) Dirk Ponsel et al.   MOLECULES

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Rapid construction and characterization of synthetic antibody libraries without DNA amplification

(2010) Xin Ge et al.   BIOTECHNOLOGY AND BIOENGINEERING

Random Mutagenesis Methods for In Vitro Directed Enzyme Evolution

(2010) Nikolaos Labrou   CURRENT PROTEIN & PEPTIDE SCIENCE

Altering the spectrum of immunoglobulin V gene somatic hypermutation by modifying the active site of AID

(2010) Meng Wang et al.   JOURNAL OF EXPERIMENTAL MEDICINE

High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

(2010) Mark Matzas et al.   NATURE BIOTECHNOLOGY

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

(2010) Sriram Kosuri et al.   NATURE BIOTECHNOLOGY

Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

(2010) John Cooper et al.   PLoS One

Error-prone PCR of a fungal xylanase for improvement of its alkaline and thermal stability

(2009) Dawn Elizabeth Stephens et al.   FEMS MICROBIOLOGY LETTERS

The Protein-Protein Interface Evolution Acts in a Similar Way to Antibody Affinity Maturation

(2009) Bohua Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Construction of a large synthetic human scFv library with six diversified CDRs and high functional diversity

(2009) Hye Young Yang et al.   MOLECULES AND CELLS

Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire

(2009) J. Glanville et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A novel solid phase technology for high-throughput gene synthesis

(2008) Jan Van den Brulle et al.   BIOTECHNIQUES

The Human Combinatorial Antibody Library HuCAL GOLD Combines Diversification of All Six CDRs According to the Natural Immune System with a Novel Display Method for Efficient Selection of High-Affinity Antibodies

(2008) Christine Rothe et al.   JOURNAL OF MOLECULAR BIOLOGY

Construction of a Large Phage-Displayed Human Antibody Domain Library with a Scaffold Based On a Newly Identified Highly Soluble, Stable Heavy Chain Variable Domain

(2008) Weizao Chen et al.   JOURNAL OF MOLECULAR BIOLOGY

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

(2008) A. E. Firth et al.   NUCLEIC ACIDS RESEARCH

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution

(2008) Aurelio Hidalgo et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Modelling the human immune response: performance of a 1011 human antibody repertoire against a broad panel of therapeutically relevant antigens

(2008) C. Lloyd et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Modifying Antibody Specificity by Chain Shuffling of VH / VLbetween Antibodies with Related Specificities

(2008) P. A. Christensen et al.   SCANDINAVIAN JOURNAL OF IMMUNOLOGY

Detection of Functionally Important Regions in “Hypothetical Proteins” of Known Structure

(2008) Guy Nimrod et al.   STRUCTURE