Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step

In vivo elimination of parental clones in general and site-directed mutagenesis

(2015) Erika G. Holland et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

Recombinant renewable polyclonal antibodies

(2014) Fortunato Ferrara et al.   mAbs

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Somatic Mutations of the Immunoglobulin Framework Are Generally Required for Broad and Potent HIV-1 Neutralization

(2013) Florian Klein et al.   CELL

AXM mutagenesis: An efficient means for the production of libraries for directed evolution of proteins

(2013) Erika G. Holland et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Deep sequencing of phage display libraries to support antibody discovery

(2013) Ulla Ravn et al.   METHODS

Designed Ankyrin Repeat Proteins (DARPins) as Novel Isoform-Specific Intracellular Inhibitors of c-Jun N-Terminal Kinases

(2012) Petra Parizek et al.   ACS Chemical Biology

Somatic mutagenesis in autoimmunity

(2012) Thiago Detanico et al.   AUTOIMMUNITY

scFv Antibody: Principles and Clinical Application

(2012) Zuhaida Asra Ahmad et al.   Clinical & Developmental Immunology

Use of micro-emulsion technology for the directed evolution of antibodies

(2012) Diane L. Buhr et al.   METHODS

Synthetic antibodies: Concepts, potential and practical considerations

(2012) S. Miersch et al.   METHODS

Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries

(2012) Renhua Huang et al.   METHODS

Protein selection using yeast surface display

(2012) Nimish Gera et al.   METHODS

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification

(2012) Tuomas Huovinen et al.   PLoS One

Targeting the SH2-Kinase Interface in Bcr-Abl Inhibits Leukemogenesis

(2011) Florian Grebien et al.   CELL

DARPins and other repeat protein scaffolds: advances in engineering and applications

(2011) Ykelien L Boersma et al.   CURRENT OPINION IN BIOTECHNOLOGY

Advantages of mRNA display selections over other selection techniques for investigation of protein–protein interactions

(2011) Hui Wang et al.   Expert Review of Proteomics

Limits for Antibody Affinity Maturation and Repertoire Diversification in Hypervaccinated Humans

(2011) Tine Rugh Poulsen et al.   JOURNAL OF IMMUNOLOGY

DARPins Recognizing the Tumor-Associated Antigen EpCAM Selected by Phage and Ribosome Display and Engineered for Multivalency

(2011) Nikolas Stefan et al.   JOURNAL OF MOLECULAR BIOLOGY

Teaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 Scaffold

(2011) Akiko Koide et al.   JOURNAL OF MOLECULAR BIOLOGY

Biotechnological applications of recombinant single-domain antibody fragments

(2011) Ario de Marco   Microbial Cell Factories

Phage Display: Selecting Straws Instead of a Needle from a Haystack

(2011) Miha Vodnik et al.   MOLECULES

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Isolation of monobodies that bind specifically to the SH3 domain of the Fyn tyrosine protein kinase

(2011) Renhua Huang et al.   New Biotechnology

Antibody fragments

(2010) Aaron L. Nelson   mAbs

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

(2010) John Wojcik et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution

(2010) Christian Zahnd et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Adnectins: engineered target-binding protein therapeutics

(2010) D. Lipovsek   PROTEIN ENGINEERING DESIGN & SELECTION

A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

(2010) Dorothy Beckett et al.   PROTEIN SCIENCE

FN3: a new protein scaffold reaches the clinic

(2009) Laird Bloom et al.   DRUG DISCOVERY TODAY

mRNA Display Selection of a High-Affinity, Modification-Specific Phospho-IκBα-Binding Fibronectin

(2008) C. Anders Olson et al.   ACS Chemical Biology

A Decade of Yeast Surface Display Technology: Where Are We Now?

(2008) Eric Shusta et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Alternative binding proteins: Anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities

(2008) Arne Skerra   FEBS Journal

The Intrinsic Contributions of Tyrosine, Serine, Glycine and Arginine to the Affinity and Specificity of Antibodies

(2008) Sara Birtalan et al.   JOURNAL OF MOLECULAR BIOLOGY

Picomolar Affinity Fibronectin Domains Engineered Utilizing Loop Length Diversity, Recursive Mutagenesis, and Loop Shuffling

(2008) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY