MDC-Analyzer: A novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites

ProxiMAX randomization: a new technology for non-degenerate saturation mutagenesis of contiguous codons

(2013) Mohammed Ashraf et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library construction

(2013) Lixia Tang et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Strategies for the discovery and engineering of enzymes for biocatalysis

(2013) Timo Davids et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Use of ‘small but smart’ libraries to enhance the enantioselectivity of an esterase fromBacillus stearothermophilustowards tetrahydrofuran-3-yl acetate

(2013) Alberto Nobili et al.   FEBS Journal

Optimal codon randomization via mathematical programming

(2013) Yuval Nov et al.   JOURNAL OF THEORETICAL BIOLOGY

Key Residues for Controlling Enantioselectivity of Halohydrin Dehalogenase from Arthrobacter sp. Strain AD2, Revealed by Structure-Guided Directed Evolution

(2012) Lixia Tang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis

(2012) Philip A. Romero et al.   ACS Synthetic Biology

Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

(2012) Sabrina Kille et al.   ACS Synthetic Biology

Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli

(2011) Jen C. Pai et al.   ANALYTICAL BIOCHEMISTRY

Natural Diversity to Guide Focused Directed Evolution

(2010) Helge Jochens et al.   CHEMBIOCHEM

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination

(2010) Pete Heinzelman et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

HotSpot Wizard: a web server for identification of hot spots in protein engineering

(2009) A. Pavelka et al.   NUCLEIC ACIDS RESEARCH

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

(2008) Joaquin Sanchis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method

(2007) Wen-Chi Tseng et al.   ANALYTICAL BIOCHEMISTRY