Biochemical mechanisms of vertebrate hedgehog signaling

A conserved molecular switch in Class F receptors regulates receptor activation and pathway selection

(2019) Shane C. Wright et al.   Nature Communications

Misactivation of Hedgehog signaling causes inherited and sporadic cancers

(2019) David R. Raleigh et al.   JOURNAL OF CLINICAL INVESTIGATION

NPC intracellular cholesterol transporter 1 (NPC1)-mediated cholesterol export from lysosomes

(2019) Suzanne R. Pfeffer   JOURNAL OF BIOLOGICAL CHEMISTRY

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates

(2018) Eamon FX Byrne et al.   CURRENT OPINION IN CELL BIOLOGY

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   DEVELOPMENTAL CELL

A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies

(2018) David K. Breslow et al.   NATURE GENETICS

Morphogen gradient reconstitution reveals Hedgehog pathway design principles

(2018) Pulin Li et al.   SCIENCE

Structural basis for the recognition of Sonic Hedgehog by human Patched1

(2018) Xin Gong et al.   SCIENCE

G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   Science Signaling

Transverse distribution of plasma membrane bilayer cholesterol: Picking sides

(2018) Theodore L. Steck et al.   TRAFFIC

Basal Suppression of the Sonic Hedgehog Pathway by the G-Protein-Coupled Receptor Gpr161 Restricts Medulloblastoma Pathogenesis

(2018) Issei S. Shimada et al.   Cell Reports

G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog

(2018) Ganesh V. Pusapati et al.   Science Signaling

Structural Basis of Smoothened Activation in Hedgehog Signaling

(2018) Pengxiang Huang et al.   CELL

Structures of human Patched and its complex with native palmitoylated sonic hedgehog

(2018) Xiaofeng Qi et al.   NATURE

Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex

(2018) Xiaofeng Qi et al.   SCIENCE

Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched

(2018) Yunxiao Zhang et al.   CELL

LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription

(2018) Amar N. Mirza et al.   CELL

From Drosophila segmentation to human cancer therapy

(2018) Philip W. Ingham   DEVELOPMENT

Cilia-Associated Oxysterols Activate Smoothened

(2018) David R. Raleigh et al.   MOLECULAR CELL

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

(2018) George Hedger et al.   STRUCTURE

Cholesterol Modification of Smoothened Is Required for Hedgehog Signaling

(2017) Xu Xiao et al.   MOLECULAR CELL

Crystal structures of theBurkholderia multivoranshopanoid transporter HpnN

(2017) Nitin Kumar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid, direct activity assays for Smoothened reveal Hedgehog pathway regulation by membrane cholesterol and extracellular sodium

(2017) Benjamin R. Myers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand

(2017) Xianjun Zhang et al.   Nature Communications

Cellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling

(2016) Pengxiang Huang et al.   CELL

Hedgehog signalling

(2016) Raymond Teck Ho Lee et al.   DEVELOPMENT

An essential role for Grk2 in Hedgehog signalling downstream of Smoothened

(2016) Zhonghua Zhao et al.   EMBO REPORTS

Smoothened determines β-arrestin–mediated removal of the G protein–coupled receptor Gpr161 from the primary cilium

(2016) Kasturi Pal et al.   JOURNAL OF CELL BIOLOGY

Structural basis of Smoothened regulation by its extracellular domains

(2016) Eamon F. X. Byrne et al.   NATURE

Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics

(2016) Bryn S. Moore et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog

(2016) Hanna Tukachinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Primary Cilia and Mammalian Hedgehog Signaling

(2016) Fiona Bangs et al.   Cold Spring Harbor Perspectives in Biology

Bimodal antagonism of PKA signalling by ARHGAP36

(2016) Rebecca L. Eccles et al.   Nature Communications

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

(2016) Giovanni Luchetti et al.   eLife

Proteomics of Primary Cilia by Proximity Labeling

(2015) David U. Mick et al.   DEVELOPMENTAL CELL

Reduced cholesterol levels impair Smoothened activation in Smith–Lemli–Opitz syndrome

(2015) Robert Blassberg et al.   HUMAN MOLECULAR GENETICS

Secreted HHIP1 interacts with heparan sulfate and regulates Hedgehog ligand localization and function

(2015) Alexander M. Holtz et al.   JOURNAL OF CELL BIOLOGY

Ciliary adenylyl cyclases control the Hedgehog pathway

(2015) L. Vuolo et al.   JOURNAL OF CELL SCIENCE

Inactivation of a Gα s –PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis

(2015) Ramiro Iglesias-Bartolome et al.   NATURE CELL BIOLOGY

Regulation of the oncoprotein Smoothened by small molecules

(2015) Hayley J Sharpe et al.   Nature Chemical Biology

An In Vivo Chemical Genetic Screen Identifies Phosphodiesterase 4 as a Pharmacological Target for Hedgehog Signaling Inhibition

(2015) Charles H. Williams et al.   Cell Reports

Phosphodiesterase 4D acts downstream of Neuropilin to control Hedgehog signal transduction and the growth of medulloblastoma

(2015) Xuecai Ge et al.   eLife

EFCAB7 and IQCE Regulate Hedgehog Signaling by Tethering the EVC-EVC2 Complex to the Base of Primary Cilia

(2014) Ganesh V. Pusapati et al.   DEVELOPMENTAL CELL

The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment

(2014) Mu He et al.   NATURE CELL BIOLOGY

The G protein α subunit Gαs is a tumor suppressor in Sonic hedgehog−driven medulloblastoma

(2014) Xuelian He et al.   NATURE MEDICINE

Arhgap36-dependent activation of Gli transcription factors

(2014) P. G. Rack et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for Smoothened receptor modulation and chemoresistance to anticancer drugs

(2014) Chong Wang et al.   Nature Communications

Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling

(2014) Pawel Niewiadomski et al.   Cell Reports

The Ciliary G-Protein-Coupled Receptor Gpr161 Negatively Regulates the Sonic Hedgehog Pathway via cAMP Signaling

(2013) Saikat Mukhopadhyay et al.   CELL

Interaction of PACAP with Sonic hedgehog reveals complex regulation of the Hedgehog pathway by PKA

(2013) Pawel Niewiadomski et al.   CELLULAR SIGNALLING

Hedgehog Pathway Modulation by Multiple Lipid Binding Sites on the Smoothened Effector of Signal Response

(2013) Benjamin R. Myers et al.   DEVELOPMENTAL CELL

GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas

(2013) Scott X. Atwood et al.   NATURE

Structure of the human smoothened receptor bound to an antitumour agent

(2013) Chong Wang et al.   NATURE

Specialized filopodia direct long-range transport of SHH during vertebrate tissue patterning

(2013) Timothy A. Sanders et al.   NATURE

Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia

(2013) Marcus Bischoff et al.   NATURE CELL BIOLOGY

Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling

(2013) Daniel Nedelcu et al.   Nature Chemical Biology

Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification

(2013) Jean B Regard et al.   NATURE MEDICINE

The mechanisms of Hedgehog signalling and its roles in development and disease

(2013) James Briscoe et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural insights into proteoglycan-shaped Hedgehog signaling

(2013) D. M. Whalen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural insights into the role of the Smoothened cysteine-rich domain in Hedgehog signalling

(2013) Rajashree Rana et al.   Nature Communications

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling

(2013) Sigrid Nachtergaele et al.   eLife

Mutations in Multidomain Protein MEGF8 Identify a Carpenter Syndrome Subtype Associated with Defective Lateralization

(2012) Stephen R.F. Twigg et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Hedgehog Partial Agonism Drives Warburg-like Metabolism in Muscle and Brown Fat

(2012) Raffaele Teperino et al.   CELL

Distinct Phosphorylations on Kinesin Costal-2 Mediate Differential Hedgehog Signaling Strength

(2012) Nadia Ranieri et al.   DEVELOPMENTAL CELL

A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary Cilia

(2012) Karolin V. Dorn et al.   DEVELOPMENTAL CELL

Scube/You activity mediates release of dually lipid-modified Hedgehog signal in soluble form

(2012) A. Creanga et al.   GENES & DEVELOPMENT

Oxysterols are allosteric activators of the oncoprotein Smoothened

(2012) Sigrid Nachtergaele et al.   Nature Chemical Biology

Structural Basis of Wnt Recognition by Frizzled

(2012) C. Y. Janda et al.   SCIENCE

Dispatched and Scube Mediate the Efficient Secretion of the Cholesterol-Modified Hedgehog Ligand

(2012) Hanna Tukachinsky et al.   Cell Reports

Gli Proteins in Development and Disease

(2011) Chi-chung Hui et al.   Annual Review of Cell and Developmental Biology

Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube

(2011) M. Tuson et al.   DEVELOPMENT

Boc and Gas1 Each Form Distinct Shh Receptor Complexes with Ptch1 and Are Required for Shh-Mediated Cell Proliferation

(2011) Luisa Izzi et al.   DEVELOPMENTAL CELL

Overlapping Roles and Collective Requirement for the Coreceptors GAS1, CDO, and BOC in SHH Pathway Function

(2011) Benjamin L. Allen et al.   DEVELOPMENTAL CELL

A Three-part Signal Governs Differential Processing of Gli1 and Gli3 Proteins by the Proteasome

(2011) Erin K. Schrader et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition

(2011) Francesc R Garcia-Gonzalo et al.   NATURE GENETICS

The Hedgehog Receptor Patched Is Involved in Cholesterol Transport

(2011) Michel Bidet et al.   PLoS One

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

(2011) Y. H. Belgacem et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sequential Phosphorylation of Smoothened Transduces Graded Hedgehog Signaling

(2011) Y. Su et al.   Science Signaling

Sonic Hedgehog Dependent Phosphorylation by CK1α and GRK2 Is Required for Ciliary Accumulation and Activation of Smoothened

(2011) Yongbin Chen et al.   PLOS BIOLOGY

Small Molecule Inhibition of GDC-0449 Refractory Smoothened Mutants and Downstream Mechanisms of Drug Resistance

(2010) Gerrit J. P. Dijkgraaf et al.   CANCER RESEARCH

Hedgehog proteins activate pro-angiogenic responses in endothelial cells through non-canonical signaling pathways

(2010) Pilar Chinchilla et al.   CELL CYCLE

The ciliary membrane

(2010) Rajat Rohatgi et al.   CURRENT OPINION IN CELL BIOLOGY

The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins

(2010) E. W. Humke et al.   GENES & DEVELOPMENT

G protein-coupled receptor kinase 2 promotes high-level Hedgehog signaling by regulating the active state of Smo through kinase-dependent and kinase-independent mechanisms in Drosophila

(2010) Y. Chen et al.   GENES & DEVELOPMENT

All Mammalian Hedgehog Proteins Interact with Cell Adhesion Molecule, Down-regulated by Oncogenes (CDO) and Brother of CDO (BOC) in a Conserved Manner

(2010) Jennifer M. Kavran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu–Gli protein complexes

(2010) Hanna Tukachinsky et al.   JOURNAL OF CELL BIOLOGY

Evaluating Smoothened as a G-protein-coupled receptor for Hedgehog signalling

(2010) Katie L. Ayers et al.   TRENDS IN CELL BIOLOGY

Structural Ties between Cholesterol Transport and Morphogen Signaling

(2009) J. Fernando Bazan et al.   CELL

Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved

(2009) M.-H. Chen et al.   GENES & DEVELOPMENT

Sonic-hedgehog-mediated proliferation requires the localization of PKA to the cilium base

(2009) M. Barzi et al.   JOURNAL OF CELL SCIENCE

Unlimited multistability in multisite phosphorylation systems

(2009) Matthew Thomson et al.   NATURE

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP

(2009) Benjamin Bishop et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The structure of SHH in complex with HHIP reveals a recognition role for the Shh pseudo active site in signaling

(2009) Ivan Bosanac et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Sonic Hedgehog Guides Axons through a Noncanonical, Src-Family-Kinase-Dependent Signaling Pathway

(2009) Patricia T. Yam et al.   NEURON

Massively parallel sequencing identifies the gene Megf8 with ENU-induced mutation causing heterotaxy

(2009) Z. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gli2 trafficking links Hedgehog-dependent activation of Smoothened in the primary cilium to transcriptional activation in the nucleus

(2009) J. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hedgehog signal transduction by Smoothened: Pharmacologic evidence for a 2-step activation process

(2009) R. Rohatgi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Hedgehog Signaling Pathway: Where Did It Come From?

(2009) George Hausmann et al.   PLOS BIOLOGY

The decoupling of Smoothened from Gαi proteins has little effect on Gli3 protein processing and Hedgehog-regulated chick neural tube patterning

(2008) Wee-Chuang Low et al.   DEVELOPMENTAL BIOLOGY

Intraflagellar transport, cilia, and mammalian Hedgehog signaling: Analysis in mouse embryonic fibroblasts

(2008) Polloneal Jymmiel R. Ocbina et al.   DEVELOPMENTAL DYNAMICS

Hhat Is a Palmitoylacyltransferase with Specificity forN-Palmitoylation of Sonic Hedgehog

(2008) John A. Buglino et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Smoothened Signaling in Vertebrates Is Facilitated by a G Protein-coupled Receptor Kinase

(2008) Melanie Philipp et al.   MOLECULAR BIOLOGY OF THE CELL

The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla

(2008) Jason S. McLellan et al.   NATURE