Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV

(2017) Xiaowu Li et al.   JOURNAL OF MOLECULAR BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

(2017) Maryam Khoshouei et al.   Nature Communications

Using the Volta phase plate with defocus for cryo-EM single particle analysis

(2017) Radostin Danev et al.   eLife

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

A Primer to Single-Particle Cryo-Electron Microscopy

(2015) Yifan Cheng et al.   CELL

Alignment of cryo-EM movies of individual particles by optimization of image translations

(2015) John L. Rubinstein et al.   JOURNAL OF STRUCTURAL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy

(2015) Benjamin A Barad et al.   NATURE METHODS

Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

(2015) Timothy Grant et al.   eLife

Near-atomic resolution reconstructions using a mid-range electron microscope operated at 200kV

(2014) Melody G. Campbell et al.   JOURNAL OF STRUCTURAL BIOLOGY

Ultrastable gold substrates for electron cryomicroscopy

(2014) C. J. Russo et al.   SCIENCE

Choice of operating voltage for a transmission electron microscope

(2014) R.F. Egerton   ULTRAMICROSCOPY

Beam-induced motion correction for sub-megadalton cryo-EM particles

(2014) Sjors HW Scheres   eLife

One number does not fit all: Mapping local variations in resolution in cryo-EM reconstructions

(2013) Giovanni Cardone et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy

(2013) Shaoxia Chen et al.   ULTRAMICROSCOPY

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

Outcome of the First Electron Microscopy Validation Task Force Meeting

(2012) Richard Henderson et al.   STRUCTURE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM

(2010) Robert M. Glaeser et al.   JOURNAL OF STRUCTURAL BIOLOGY

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Appion: An integrated, database-driven pipeline to facilitate EM image processing

(2009) Gabriel C. Lander et al.   JOURNAL OF STRUCTURAL BIOLOGY

DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy

(2009) N.R. Voss et al.   JOURNAL OF STRUCTURAL BIOLOGY

Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes

(2008) Robert A Grassucci et al.   Nature Protocols