While the revolution will not be crystallized, biochemistry reigns supreme

The Current Revolution in Cryo-EM

(2016) Edward H. Egelman   BIOPHYSICAL JOURNAL

Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating

(2016) Doreen Matthies et al.   CELL

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

A Nonoligomerizing Mutant Form of Helicobacter pylori VacA Allows Structural Analysis of the p33 Domain

(2016) Christian González-Rivera et al.   INFECTION AND IMMUNITY

Continuous changes in structure mapped by manifold embedding of single-particle data in cryo-EM

(2016) Joachim Frank et al.   METHODS

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution

(2016) Thi Hoang Duong Nguyen et al.   NATURE

Cryo-EM structure of the spliceosome immediately after branching

(2016) Wojciech P. Galej et al.   NATURE

Structure of transcribing mammalian RNA polymerase II

(2016) Carrie Bernecky et al.   NATURE

Near-atomic resolution visualization of human transcription promoter opening

(2016) Yuan He et al.   NATURE

Transcription initiation complex structures elucidate DNA opening

(2016) C. Plaschka et al.   NATURE

Structure of promoter-bound TFIID and model of human pre-initiation complex assembly

(2016) Robert K. Louder et al.   NATURE

Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution

(2016) Julian von der Ecken et al.   NATURE

Molecular architecture of the complete COG tethering complex

(2016) Jun Yong Ha et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Near-atomic cryo-EM structure of PRC1 bound to the microtubule

(2016) Elizabeth H. Kellogg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for integration of GluD receptors within synaptic organizer complexes

(2016) Jonathan Elegheert et al.   SCIENCE

Molecular architecture of the human U4/U6.U5 tri-snRNP

(2016) D. E. Agafonov et al.   SCIENCE

The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis

(2016) R. Wan et al.   SCIENCE

Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hsp90 traps and stabilizes an unfolded kinase

(2016) K. A. Verba et al.   SCIENCE

Structure of a yeast catalytic step I spliceosome at 3.4 A resolution

(2016) R. Wan et al.   SCIENCE

Structure of a yeast activated spliceosome at 3.5 A resolution

(2016) C. Yan et al.   SCIENCE

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

(2016) S. Banerjee et al.   SCIENCE

Topology and structure of an engineered human cohesin complex bound to Pds5B

(2016) Michael T. Hons et al.   Nature Communications

RNA polymerase I–Rrn3 complex at 4.8 Å resolution

(2016) Christoph Engel et al.   Nature Communications

Structure of the initiation-competent RNA polymerase I and its implication for transcription

(2016) Michael Pilsl et al.   Nature Communications

Molecular architecture of the complete COG tethering complex

(2016) Jun Yong Ha et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

2.8-Å Cryo-EM Structure of the Large Ribosomal Subunit from the Eukaryotic Parasite Leishmania

(2016) Moran Shalev-Benami et al.   Cell Reports

Overview and future of single particle electron cryomicroscopy

(2015) Richard Henderson   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Cryo-Electron Microscopy and the Amazing Race to Atomic Resolution

(2015) Elad Binshtein et al.   BIOCHEMISTRY

A Primer to Single-Particle Cryo-Electron Microscopy

(2015) Yifan Cheng et al.   CELL

Single-Particle Cryo-EM at Crystallographic Resolution

(2015) Yifan Cheng   CELL

Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex

(2015) N. Rigo et al.   EMBO JOURNAL

Directly reconstructing principal components of heterogeneous particles from cryo-EM images

(2015) Hemant D. Tagare et al.   JOURNAL OF STRUCTURAL BIOLOGY

Protein domain mapping by internal labeling and single particle electron microscopy

(2015) Claudio Ciferri et al.   JOURNAL OF STRUCTURAL BIOLOGY

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

(2015) Thi Hoang Duong Nguyen et al.   NATURE

Structure of the E. coli ribosome–EF-Tu complex at

(2015) Niels Fischer et al.   NATURE

Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase

(2015) Jianhua Zhao et al.   NATURE

The revolution will not be crystallized: a new method sweeps through structural biology

(2015) Ewen Callaway   NATURE

Cryo-electron microscopy structure of the Slo2.2 Na+-activated K+ channel

(2015) Richard K. Hite et al.   NATURE

Atomic structure of anthrax protective antigen pore elucidates toxin translocation

(2015) Jiansen Jiang et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

(2015) Ashwin Chari et al.   NATURE METHODS

Structural basis of pre-mRNA splicing

(2015) Jing Hang et al.   SCIENCE

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

2.2 A resolution cryo-EM structure of  -galactosidase in complex with a cell-permeant inhibitor

(2015) A. Bartesaghi et al.   SCIENCE

The structure of the dynactin complex and its interaction with dynein

(2015) L. Urnavicius et al.   SCIENCE

How cryo-EM is revolutionizing structural biology

(2015) Xiao-chen Bai et al.   TRENDS IN BIOCHEMICAL SCIENCES

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

(2015) Melody G Campbell et al.   eLife

Principles of cryo-EM single-particle image processing

(2015) Fred J. Sigworth   Microscopy

Sample preparation of biological macromolecular assemblies for the determination of high-resolution structures by cryo-electron microscopy

(2015) Holger Stark et al.   Microscopy

Generalized single-particle cryo-EM – a historical perspective

(2015) Joachim Frank   Microscopy

Architecture of the Nitric-oxide Synthase Holoenzyme Reveals Large Conformational Changes and a Calmodulin-driven Release of the FMN Domain

(2014) Adam L. Yokom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of a mammalian ryanodine receptor

(2014) Ran Zalk et al.   NATURE

Three-dimensional structure of human γ-secretase

(2014) Peilong Lu et al.   NATURE

Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution

(2014) Zhen Yan et al.   NATURE

Structure of the F-actin–tropomyosin complex

(2014) Julian von der Ecken et al.   NATURE

Architecture and conformational switch mechanism of the ryanodine receptor

(2014) Rouslan G. Efremov et al.   NATURE

Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex

(2014) Sulochanadevi Baskaran et al.   eLife

High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation

(2014) Zhiguo Shang et al.   eLife

Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

(2014) Matteo Allegretti et al.   eLife

Likelihood-based classification of cryo-EM images using FREALIGN

(2013) Dmitry Lyumkis et al.   JOURNAL OF STRUCTURAL BIOLOGY

Xmipp 3.0: An improved software suite for image processing in electron microscopy

(2013) J.M. de la Rosa-Trevín et al.   JOURNAL OF STRUCTURAL BIOLOGY

TRPV1 structures in distinct conformations reveal activation mechanisms

(2013) Erhu Cao et al.   NATURE

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Finding trimeric HIV-1 envelope glycoproteins in random noise

(2013) M. van Heel   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Avoiding the pitfalls of single particle cryo-electron microscopy: Einstein from noise

(2013) R. Henderson   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular assembly of botulinum neurotoxin progenitor complexes

(2013) D. A. Benefield et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Analysis of the Oligomeric States of Helicobacter pylori VacA Toxin

(2012) Melissa G. Chambers et al.   JOURNAL OF MOLECULAR BIOLOGY

SIMPLE: Software for ab initio reconstruction of heterogeneous single-particles

(2012) Dominika Elmlund et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps

(2012) Qiu Wang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex

(2012) E. Wolf et al.   RNA

DOLORS: Versatile Strategy for Internal Labeling and Domain Localization in Electron Microscopy

(2012) Pick-Wei Lau et al.   STRUCTURE

Iterative Stable Alignment and Clustering of 2D Transmission Electron Microscope Images

(2012) Zhengfan Yang et al.   STRUCTURE

Molecular architecture of human polycomb repressive complex 2

(2012) Claudio Ciferri et al.   eLife

Cryo-EM-the first thirty years

(2011) J. DUBOCHET   JOURNAL OF MICROSCOPY

Tilt-Pair Analysis of Images from a Range of Different Specimens in Single-Particle Electron Cryomicroscopy

(2011) Richard Henderson et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure and nucleosome interaction of the yeast NuA4 and Piccolo–NuA4 histone acetyltransferase complexes

(2011) Johnathan R Chittuluru et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

3D Mapping of the SPRY2 Domain of Ryanodine Receptor 1 by Single-Particle Cryo-EM

(2011) Alex Perálvarez-Marín et al.   PLoS One

Structural flexibility of the G s  -helical domain in the  2-adrenoceptor Gs complex

(2011) G. H. Westfield et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identifying Conformational States of Macromolecules by Eigen-Analysis of Resampled Cryo-EM Images

(2011) Pawel A. Penczek et al.   STRUCTURE

Possibilities of subunit localization with fluorescent protein tags and electron microscopy examplified by a cyanobacterial NDH-1 study

(2010) Mariam Birungi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Do main location within the cystic fibrosis transmembrane conductance regulator protein investigated by electron microscopy and gold labelling

(2010) Liang Zhang et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label

(2010) Dirk Flemming et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Molecular architecture of the TRAPPII complex and implications for vesicle tethering

(2010) Calvin K Yip et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural organization of the functional domains of Clostridium difficile toxins A and B

(2010) R. N. Pruitt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

(2010) J. R. A. Hutchins et al.   SCIENCE

Topology and Organization of the Salmonella typhimurium Type III Secretion Needle Complex Components

(2010) Oliver Schraidt et al.   PLoS Pathogens

Exon, intron and splice site locations in the spliceosomal B complex

(2009) Elmar Wolf et al.   EMBO JOURNAL

Localization and orientation of the γ-Tubulin Small Complex components using protein tags as labels for single particle EM

(2009) Rebeca M. Choy et al.   JOURNAL OF STRUCTURAL BIOLOGY

Radiation damage effects at four specimen temperatures from 4 to 100K

(2009) Benjamin E. Bammes et al.   JOURNAL OF STRUCTURAL BIOLOGY

SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs

(2009) Tanvir R Shaikh et al.   Nature Protocols

A protein-based EM label for RNA identifies the location of exons in spliceosomes

(2008) Eric A Alcid et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Saccharomyces cerevisiae septins: Supramolecular organization of heterooligomers and the mechanism of filament assembly

(2008) A. Bertin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Actin filament labels for localizing protein components in large complexes viewed by electron microscopy

(2008) M. E. Stroupe et al.   RNA