CRISPR-mediated acceleration of wheat improvement: advances and perspectives

Large-scale genome editing in plants: approaches, applications, and future perspectives

(2023) Tianzhen Liu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase

(2023) Huawei Tong et al.   NATURE BIOTECHNOLOGY

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

(2023) Lei Yang et al.   NATURE BIOTECHNOLOGY

An elite γ‐gliadin allele improves end‐use quality in wheat

(2023) Dan Liu et al.   NEW PHYTOLOGIST

CRISPR /Cas9‐targeted mutagenesis of TaDCL4 , TaDCL5 and TaRDR6 induces male sterility in common wheat

(2023) Rongzhi Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR /Cas9‐guided knockout of eIF4E improves Wheat yellow mosaic virus resistance without yield penalty

(2023) Jinhong Kan et al.   PLANT BIOTECHNOLOGY JOURNAL

Dosage differences in 12-OXOPHYTODIENOATE REDUCTASE genes modulate wheat root growth

(2023) Gilad Gabay et al.   Nature Communications

CRISPR/Cas9 editing of wheat Ppd-1 gene homoeologs alters spike architecture and grain morphometric traits

(2023) Aliya Errum et al.   FUNCTIONAL & INTEGRATIVE GENOMICS

DIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by de‐phosphorylating TaSnRK1.1 in wheat

(2023) Jingyi Wang et al.   Journal of Integrative Plant Biology

Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat

(2023) Long Song et al.   NATURE

Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases

(2023) George D. Lampe et al.   NATURE BIOTECHNOLOGY

Precise integration of large DNA sequences in plant genomes using PrimeRoot editors

(2023) Chao Sun et al.   NATURE BIOTECHNOLOGY

Efficient protein tagging and cis-regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants

(2023) Jitesh Kumar et al.   PLANT CELL

An efficient transformation method for genome editing of elite bread wheat cultivars

(2023) Akshaya K. Biswal et al.   Frontiers in Plant Science

Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

(2023) Cheng Chang et al.   Genes

Genetic modification of Gγ subunit AT1 enhances salt-alkali tolerance in main graminaceous crops

(2023) Wenjing Sun et al.   National Science Review

Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance

(2023) Chen Zhang et al.   Journal of Integrative Plant Biology

Alternative splicing of TaHSFA6e modulates heat shock protein–mediated translational regulation in response to heat stress in wheat

(2023) Jingjing Wen et al.   NEW PHYTOLOGIST

Grain yield improvement by genome editing of TaARF12 that decoupled peduncle and rachis development trajectories via differential regulation of gibberellin signalling in wheat

(2023) Xingchen Kong et al.   PLANT BIOTECHNOLOGY JOURNAL

Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors

(2023) Ao Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Thermosensitive SUMOylation of TaHsfA1 defines a dynamic ON/OFF molecular switch for the heat stress response in wheat

(2023) Haoran Wang et al.   PLANT CELL

Genome-edited powdery mildew resistance in wheat without growth penalties

(2022) Shengnan Li et al.   NATURE

Simultaneous editing of host factor gene TaPDIL5‐1 homoeoalleles confers wheat yellow mosaic virus resistance in hexaploid wheat

(2022) Jinhong Kan et al.   NEW PHYTOLOGIST

A single nucleotide deletion in the third exon of FT‐D1 increases the spikelet number and delays heading date in wheat ( Triticum aestivum L.)

(2022) Zhaoyan Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

An engineered prime editor with enhanced editing efficiency in plants

(2022) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Development and optimization of a Barley stripe mosaic virus ‐mediated gene editing system to improve Fusarium head blight resistance in wheat

(2022) Hui Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

Breeding future crops to feed the world through de novo domestication

(2022) Hong Yu et al.   Nature Communications

The future of gene-edited crops in China

(2022) Jian-Kang Zhu   National Science Review

Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi

(2022) Ning Wang et al.   CELL

When domestication bottleneck meets weed

(2022) Yanqing Sun et al.   Molecular Plant

Histone acetyltransferase TaHAG1 interacts with TaNACL to promote heat stress tolerance in wheat

(2022) Jingchen Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed promoter and gene editing in wheat using a virus‐based guide RNA delivery system

(2022) Wei Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Centromeres: From chromosome biology to biotechnology applications and synthetic genomes in plants

(2022) Jingwei Zhou et al.   PLANT BIOTECHNOLOGY JOURNAL

Dispersed emergence and protracted domestication of polyploid wheat uncovered by mosaic ancestral haploblock inference

(2022) Zihao Wang et al.   Nature Communications

Developments and prospects for doubled haploid wheat

(2022) Serik Eliby et al.   BIOTECHNOLOGY ADVANCES

Genome edited wheat- current advances for the second green revolution

(2022) Muhammad Jawad Akbar Awan et al.   BIOTECHNOLOGY ADVANCES

Genetic control of grain appearance quality in rice

(2022) Dongsheng Zhao et al.   BIOTECHNOLOGY ADVANCES

Base editing‐mediated targeted evolution of ACCase for herbicide‐resistant rice mutants

(2022) Hongzhi Wang et al.   Journal of Integrative Plant Biology

Simultaneous editing of three homoeologs of TaCIPK14 confers broad‐spectrum resistance to stripe rust in wheat

(2022) Fuxin He et al.   PLANT BIOTECHNOLOGY JOURNAL

Wheat genomic study for genetic improvement of traits in China

(2022) Jun Xiao et al.   Science China-Life Sciences

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

(2022) Naglaa A. Abdallah et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Public perception of plant gene technologies worldwide in the light of food security

(2022) Woźniak-Gientka Ewa et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

The Power and Perils of De Novo Domestication Using Genome Editing

(2022) Madelaine E. Bartlett et al.   Annual Review of Plant Biology

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

(2022) Matthew T. N. Yarnall et al.   NATURE BIOTECHNOLOGY

Antagonistic control of seed dormancy in rice by two bHLH transcription factors

(2022) Fan Xu et al.   NATURE GENETICS

CRISPR /Cas9‐mediated restoration of Tamyb10 to create pre‐harvest sprouting‐resistant red wheat

(2022) Yiwang Zhu et al.   PLANT BIOTECHNOLOGY JOURNAL

Precise in planta genome editing via homology‐directed repair in wheat

(2022) Weifeng Luo et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR ‐induced miRNA156 ‐recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat

(2022) Ajay Gupta et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome‐wide association study reveals structural chromosome variations with phenotypic effects in wheat ( Triticum aestivum L.)

(2022) Jiajia Zhao et al.   PLANT JOURNAL

Genome engineering for crop improvement and future agriculture

(2021) Caixia Gao   CELL

A route to de novo domestication of wild allotetraploid rice

(2021) Hong Yu et al.   CELL

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

(2021) Sarah Raffan et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing in plants with MAD7 nuclease

(2021) Qiupeng Lin et al.   Journal of Genetics and Genomics

Pyramiding favorable alleles in an elite wheat variety in one generation by CRISPR/Cas9-mediated multiplex gene editing

(2021) Jinman Luo et al.   Molecular Plant

FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat

(2021) Dejie Du et al.   NEW PHYTOLOGIST

Genome editing reagent delivery in plants

(2021) Rishikesh Ghogare et al.   TRANSGENIC RESEARCH

Genetic manipulations of TaARE1 boost nitrogen utilization and grain yield in wheat

(2021) Meng Guo et al.   Journal of Genetics and Genomics

Large chromosomal segment deletions by CRISPR/LbCpf1‐mediated multiplex gene editing in soybean

(2021) Kaixuan Duan et al.   Journal of Integrative Plant Biology

Development of an efficient plant dual cytosine and adenine editor

(2021) Rongfang Xu et al.   Journal of Integrative Plant Biology

Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9

(2021) Jiahui Zhang et al.   Journal of Integrative Plant Biology

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

(2021) Tingdong Li et al.   Molecular Plant

CRISPR‐Cereal: a guide RNA design tool integrating regulome and genomic variation for wheat, maize and rice

(2021) Chao He et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9‐mediated genome editing for wheat grain quality improvement

(2021) Shujuan Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

(2021) Wei Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing

(2021) Fengti Qiu et al.   Science China-Life Sciences

CRISPR/Cas9 mediated disruption of Inositol Pentakisphosphate 2- Kinase 1 (TaIPK1) reduces phytic acid and improves iron and zinc accumulation in wheat grains

(2021) Saira Ibrahim et al.   Journal of Advanced Research

Shaping polyploid wheat for success: Origins, domestication, and the genetic improvement of agronomic traits

(2021) Jie Liu et al.   Journal of Integrative Plant Biology

WheatOmics: A platform combining multiple omics data to accelerate functional genomics studies in wheat

(2021) Shengwei Ma et al.   Molecular Plant

Precise genomic deletions using paired prime editing

(2021) Junhong Choi et al.   NATURE BIOTECHNOLOGY

Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing

(2021) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize

(2020) Haijun Liu et al.   PLANT CELL

CRISPR/Cas9 mediated disruption of TaNP1 genes results in complete male sterility in bread wheat

(2020) Jian Li et al.   Journal of Genetics and Genomics

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton ( G.hirsutum ) plants

(2020) Qiongqiong Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification of an elite wheat-rye T1RS·1BL translocation line conferring high resistance to powdery mildew and stripe rust

(2020) Guohao Han et al.   PLANT DISEASE

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9

(2020) Shengxing Wang et al.   NATURE BIOTECHNOLOGY

Glycosylase base editors enable C-to-A and C-to-G base changes

(2020) Dongdong Zhao et al.   NATURE BIOTECHNOLOGY

Developing a novel artificial rice germplasm for dinitroaniline herbicide resistance by base editing of OsTubA2

(2020) Lang Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Roadmap for Accelerated Domestication of an Emerging Perennial Grain Crop

(2020) Lee DeHaan et al.   TRENDS IN PLANT SCIENCE

CRISPR/Cas9 editing of wheat TaQ genes alters spike morphogenesis and grain threshability

(2020) Huiyun Liu et al.   Journal of Genetics and Genomics

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

(2020) Juan M. Debernardi et al.   NATURE BIOTECHNOLOGY

Fertility recovery of wheat male sterility controlled by Ms2 using CRISPR/Cas9

(2020) Huali Tang et al.   PLANT BIOTECHNOLOGY JOURNAL

Origin and adaptation to high altitude of Tibetan semi-wild wheat

(2020) Weilong Guo et al.   Nature Communications

A reporter for noninvasively monitoring gene expression and plant transformation

(2020) Yubing He et al.   Horticulture Research

Efficient multiplex genome editing by CRISPR/Cas9 in common wheat

(2020) Jihu Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9

(2020) Jingying Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Review: Revealing the genetic mechanisms of pre-harvest sprouting in hexaploid wheat (Triticum aestivum L.)

(2019) Justin M. Vetch et al.   PLANT SCIENCE

CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production

(2019) Anzu Okada et al.   PLANT BIOTECHNOLOGY JOURNAL

Development of an Agrobacterium -delivered CRISPR/Cas9 system for wheat genome editing

(2019) Zhengzhi Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

(2019) Kunling Chen et al.   Annual Review of Plant Biology

A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat

(2019) Zhenqi Su et al.   NATURE GENETICS

Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat

(2019) Chenxu Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene editing of the wheat homologs of TONNEAU 1‐recruiting motif encoding gene affects grain shape and weight in wheat

(2019) Wei Wang et al.   PLANT JOURNAL

RNA-guided DNA insertion with CRISPR-associated transposases

(2019) Jonathan Strecker et al.   SCIENCE

Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat

(2019) Fumitaka Abe et al.   Cell Reports

Generation of a multiplex mutagenesis population via pooled CRISPR ‐Cas9 in soya bean

(2019) Mengyan Bai et al.   PLANT BIOTECHNOLOGY JOURNAL

WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat

(2019) Dustin Cram et al.   BMC PLANT BIOLOGY

Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system

(2019) Huiyun Liu et al.   JOURNAL OF EXPERIMENTAL BOTANY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Recent developments and applications of genetic transformation and genome editing technologies in wheat

(2019) Ke Wang et al.   THEORETICAL AND APPLIED GENETICS

Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

(2018) Keith Lowe et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Zinc finger nuclease-mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum ) using a DNA repair template

(2018) Yidong Ran et al.   PLANT BIOTECHNOLOGY JOURNAL

Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits

(2018) Yi Zhang et al.   PLANT JOURNAL

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.)

(2018) Manjit Singh et al.   PLANT MOLECULAR BIOLOGY

True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template

(2018) Sylvia de Pater et al.   Scientific Reports

Magnesium Increases Homoeologous Crossover Frequency During Meiosis in ZIP4 (Ph1 Gene) Mutant Wheat-Wild Relative Hybrids

(2018) María-Dolores Rey et al.   Frontiers in Plant Science

Shifting the limits in wheat research and breeding using a fully annotated reference genome

(2018) et al.   SCIENCE

Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat

(2018) Shujuan Zhang et al.   BMC PLANT BIOLOGY

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Domestication of wild tomato is accelerated by genome editing

(2018) Tingdong Li et al.   NATURE BIOTECHNOLOGY

De novo domestication of wild tomato using genome editing

(2018) Agustin Zsögön et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 genome editing in wheat

(2017) Dongjin Kim et al.   FUNCTIONAL & INTEGRATIVE GENOMICS

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

(2017) Xiangbing Meng et al.   Molecular Plant

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

(2017) Susana Sánchez-León et al.   PLANT BIOTECHNOLOGY JOURNAL

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

(2017) Javier Gil-Humanes et al.   PLANT JOURNAL

Current and future editing reagent delivery systems for plant genome editing

(2017) Yidong Ran et al.   Science China-Life Sciences

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

CRISPR-edited crops free to enter market, skip regulation

(2016) Emily Waltz   NATURE BIOTECHNOLOGY

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

(2016) Shengdar Q. Tsai et al.   NATURE REVIEWS GENETICS

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

An Effective Strategy for Reliably Isolating Heritable and Cas9 -Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing

(2016) Xiuhua Gao et al.   PLANT PHYSIOLOGY

Alanine aminotransferase controls seed dormancy in barley

(2016) Kazuhiro Sato et al.   Nature Communications

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Engineered minichromosomes in plants

(2015) James A. Birchler   CHROMOSOME RESEARCH

Genomics as the key to unlocking the polyploid potential of wheat

(2015) Philippa Borrill et al.   NEW PHYTOLOGIST

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Genome editing in rice and wheat using the CRISPR/Cas system

(2014) Qiwei Shan et al.   Nature Protocols

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice

(2012) Ayako Nishizawa-Yokoi et al.   NEW PHYTOLOGIST

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Evolution in Action: Plants Resistant to Herbicides

(2010) Stephen B. Powles et al.   Annual Review of Plant Biology

Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance

(2009) Stefano Pavan et al.   MOLECULAR BREEDING

Regulation of DNA repair throughout the cell cycle

(2008) Dana Branzei et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Marker-assisted selection: an approach for precision plant breeding in the twenty-first century

(2007) B. C.Y Collard et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES