Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

(2021) Yingxiao Zhang et al.   Nature Communications

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

Precise gene replacement in rice by RNA transcript-templated homologous recombination

(2019) Shaoya Li et al.   NATURE BIOTECHNOLOGY

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

Cas9-NG greatly expands the targeting scope of genome-editing toolkit by recognizing NG and other atypical PAMs in rice

(2019) Bin Ren et al.   Molecular Plant

Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

(2019) Aimee A. Malzahn et al.   BMC BIOLOGY

Plant genome editing using xCas9 with expanded PAM compatibility

(2019) Jingying Li et al.   Journal of Genetics and Genomics

Assessment of Cas12a‐mediated gene editing efficiency in plants

(2019) Joan Miquel Bernabé‐Orts et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene editing of the wheat homologs of TONNEAU 1‐recruiting motif encoding gene affects grain shape and weight in wheat

(2019) Wei Wang et al.   PLANT JOURNAL

Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system

(2019) Huiyun Liu et al.   JOURNAL OF EXPERIMENTAL BOTANY

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

(2019) Patrick Schindele et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR‐Cas12a enables efficient biallelic gene targeting in rice

(2019) Shaoya Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice

(2019) Dongchang Zeng et al.   PLANT BIOTECHNOLOGY JOURNAL

Synthesis-dependent repair of Cpf1-induced double strand DNA breaks enables targeted gene replacement in rice

(2018) Shaoya Li et al.   JOURNAL OF EXPERIMENTAL BOTANY

Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

(2018) Mugui Wang et al.   Journal of Integrative Plant Biology

A Novel QTL q TGW3 Encodes the GSK3/SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice

(2018) Zejun Hu et al.   Molecular Plant

Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites

(2018) Zhaohui Zhong et al.   Molecular Plant

Expanding the Scope of CRISPR/Cpf1-Mediated Genome Editing in Rice

(2018) Shaoya Li et al.   Molecular Plant

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Shifting the limits in wheat research and breeding using a fully annotated reference genome

(2018) et al.   SCIENCE

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Gene editing and mutagenesis reveal inter-cultivar differences and additivity in the contribution of TaGW2 homoeologues to grain size and weight in wheat

(2018) Wei Wang et al.   THEORETICAL AND APPLIED GENETICS

Targeted mutagenesis in rice using CRISPR-Cpf1 system

(2017) Xixun Hu et al.   Journal of Genetics and Genomics

Natural Variation in the Promoter of GSE5 Contributes to Grain Size Diversity in Rice

(2017) Penggen Duan et al.   Molecular Plant

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

(2017) Mugui Wang et al.   Molecular Plant

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells

(2017) Mengjun Tu et al.   NUCLEIC ACIDS RESEARCH

Generation of targeted mutant rice using a CRISPR-Cpf1 system

(2017) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice

(2017) Xiaojia Yin et al.   PLANT CELL REPORTS

CRISPR/Cpf1-mediated DNA-free plant genome editing

(2017) Hyeran Kim et al.   Nature Communications

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases

(2017) Matthew B. Begemann et al.   Scientific Reports

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

(2016) Daesik Kim et al.   NATURE BIOTECHNOLOGY

Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida

(2016) Akira Endo et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Draft genome of the wheat A-genome progenitor Triticum urartu

(2013) Hong-Qing Ling et al.   NATURE

An-1 Encodes a Basic Helix-Loop-Helix Protein That Regulates Awn Development, Grain Size, and Grain Number in Rice

(2013) J. Luo et al.   PLANT CELL

Identification of Wheat Gene Sr35 That Confers Resistance to Ug99 Stem Rust Race Group

(2013) C. Saintenac et al.   SCIENCE

Control of grain size, shape and quality by OsSPL16 in rice

(2012) Shaokui Wang et al.   NATURE GENETICS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Linking differential domain functions of the GS3 protein to natural variation of grain size in rice

(2010) H. Mao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA