Large-scale genome editing in plants: approaches, applications, and future perspectives

Inference of CRISPR Edits from Sanger Trace Data

(2022) David Conant et al.   CRISPR Journal

Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size

(2022) Xiaoguang Song et al.   NATURE BIOTECHNOLOGY

Efficient C‐to‐G editing in rice using an optimized base editor

(2022) Yifu Tian et al.   PLANT BIOTECHNOLOGY JOURNAL

Dissecting Plant Gene Functions Using CRISPR Toolsets for Crop Improvement

(2022) Rui-Xiang Zhang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

High‐throughput genome editing in rice with a virus‐based surrogate system

(2022) Yifu Tian et al.   Journal of Integrative Plant Biology

PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants

(2021) Sebastian M. Siegner et al.   BMC BIOINFORMATICS

Genome engineering for crop improvement and future agriculture

(2021) Caixia Gao   CELL

Conserved pleiotropy of an ancient plant homeobox gene uncovered by cis-regulatory dissection

(2021) Anat Hendelman et al.   CELL

High-efficiency and multiplex adenine base editing in plants using new TadA variants

(2021) Daqi Yan et al.   Molecular Plant

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

(2021) Jonathan Y. Hsu et al.   Nature Communications

High-efficiency prime editing with optimized, paired pegRNAs in plants

(2021) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

(2021) Tingdong Li et al.   Molecular Plant

Improved plant cytosine base editors with high editing activity, purity, and specificity

(2021) Qiurong Ren et al.   PLANT BIOTECHNOLOGY JOURNAL

Five OsS40 Family Members Are Identified as Senescence-Related Genes in Rice by Reverse Genetics Approach

(2021) Habiba et al.   Frontiers in Plant Science

A FLASH pipeline for arrayed CRISPR library construction and the gene function discovery of rice receptor-like kinases

(2021) Kaiyuan Chen et al.   Molecular Plant

Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms

(2020) Yongjie Kuang et al.   Molecular Plant

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize

(2020) Haijun Liu et al.   PLANT CELL

Creating novel Wx alleles with fine‐tuned amylose levels and improved grain quality in rice by promoter editing using CRISPR/Cas9 system

(2020) Lichun Huang et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9

(2020) Shengxing Wang et al.   NATURE BIOTECHNOLOGY

Targeted, efficient sequence insertion and replacement in rice

(2020) Yuming Lu et al.   NATURE BIOTECHNOLOGY

Quantitative regulation of Waxy expression by CRISPR/Cas9‐based promoter and 5'UTR‐intron editing improves grain quality in rice

(2020) Dongchang Zeng et al.   PLANT BIOTECHNOLOGY JOURNAL

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

(2020) Juan M. Debernardi et al.   NATURE BIOTECHNOLOGY

Potato virus X Vector-Mediated DNA-Free genome editing in plants

(2020) Hirotaka Ariga et al.   PLANT AND CELL PHYSIOLOGY

A web tool for the design of prime-editing guide RNAs

(2020) Ryan D. Chow et al.   Nature Biomedical Engineering

Rice Protein Tagging Project: A Call for International Collaborations on Genome-wide In-Locus Tagging of Rice Proteins

(2020) Yuming Lu et al.   Molecular Plant

CRISPResso2 provides accurate and rapid genome editing sequence analysis

(2019) Kendell Clement et al.   NATURE BIOTECHNOLOGY

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

Accurate analysis of genuine CRISPR editing events with ampliCan

(2019) Kornel Labun et al.   GENOME RESEARCH

Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal genomic studies

(2019) Qiang Gao et al.   NEW PHYTOLOGIST

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Generation of a multiplex mutagenesis population via pooled CRISPR ‐Cas9 in soya bean

(2019) Mengyan Bai et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant gene editing through de novo induction of meristems

(2019) Michael F. Maher et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

(2019) Hakim Manghwar et al.   TRENDS IN PLANT SCIENCE

CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

(2018) Jean-Paul Concordet et al.   NUCLEIC ACIDS RESEARCH

Pea early-browning virus -mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis

(2018) Zahir Ali et al.   VIRUS RESEARCH

Web-based design and analysis tools for CRISPR base editing

(2018) Gue-Ho Hwang et al.   BMC BIOINFORMATICS

Development of Beet necrotic yellow vein virus -based vectors for multiple-gene expression and guide RNA delivery in plant genome editing

(2018) Ning Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems

(2018) Qing Liu et al.   Science China-Life Sciences

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

(2017) Hao Liu et al.   Molecular Plant

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

(2017) Xiangbing Meng et al.   Molecular Plant

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

(2017) Thomas B. Jacobs et al.   PLANT PHYSIOLOGY

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map

(2017) Ian Smith et al.   PLOS BIOLOGY

DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations

(2015) Weizhi Liu et al.   Molecular Plant

High-throughput functional genomics using CRISPR–Cas9

(2015) Ophir Shalem et al.   NATURE REVIEWS GENETICS