Structure and mechanism in non-homologous end joining

Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining

(2023) Christopher J. Buehl et al.   MOLECULAR CELL

Pol X DNA polymerases contribute to NHEJ flexibility

(2023) Michael R. Lieber   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Human DNA-dependent protein kinase activation mechanism

(2023) Shikang Liang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cryo-EM visualization of DNA-PKcs structural intermediates in NHEJ

(2023) Siyu Chen et al.   Science Advances

PAXX binding to the NHEJ machinery explains functional redundancy with XLF

(2023) Murielle Seif-El-Dahan et al.   Science Advances

Structural analysis of the basal state of the Artemis:DNA-PKcs complex

(2022) Go Watanabe et al.   NUCLEIC ACIDS RESEARCH

The importance of DNAPKcs for blunt DNA end joining is magnified when XLF is weakened

(2022) Metztli Cisneros-Aguirre et al.   Nature Communications

Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF

(2022) Ivan Corbeski et al.   Science Advances

Polλ promotes microhomology-mediated end-joining

(2022) Gurushankar Chandramouly et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining

(2022) Sara De Bragança et al.   Cell Reports

Structure of an activated DNA-PK and its implications for NHEJ

(2021) Xuemin Chen et al.   MOLECULAR CELL

Repair of DNA Double-Strand Breaks by the Nonhomologous End Joining Pathway

(2021) Benjamin M. Stinson et al.   Annual Review of Biochemistry

SAP domain forms a flexible part of DNA aperture in Ku70/80

(2021) Aleš Hnízda et al.   FEBS Journal

FUS-dependent liquid–liquid phase separation is important for DNA repair initiation

(2021) Brunno R. Levone et al.   JOURNAL OF CELL BIOLOGY

DNA damage—how and why we age?

(2021) Matt Yousefzadeh et al.   eLife

The Multifaceted Roles of Ku70/80

(2021) Sayma Zahid et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Structural basis of long-range to short-range synaptic transition in NHEJ

(2021) Siyu Chen et al.   NATURE

Long noncoding RNA NIHCOLE promotes ligation efficiency of DNA double-strand breaks in hepatocellular carcinoma

(2021) Juan P. Unfried et al.   CANCER RESEARCH

Cryo-EM of NHEJ supercomplexes provides insights into DNA repair

(2021) Amanda K. Chaplin et al.   MOLECULAR CELL

Cellular functions of the protein kinase ATM and their relevance to human disease

(2021) Ji-Hoon Lee et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural and mechanistic insights into the Artemis endonuclease and strategies for its inhibition

(2021) Yuliana Yosaatmadja et al.   NUCLEIC ACIDS RESEARCH

Nonhomologous end joining: new accessory factors fine tune the machinery

(2021) Dipayan Ghosh et al.   TRENDS IN GENETICS

ATM’s Role in the Repair of DNA Double-Strand Breaks

(2021) Atsushi Shibata et al.   Genes

To Join or Not to Join: Decision Points Along the Pathway to Double-Strand Break Repair vs. Chromosome End Protection

(2021) Stephanie M. Ackerson et al.   Frontiers in Cell and Developmental Biology

Reconsidering pathway choice: a sequential model of mammalian DNA double-strand break pathway decisions

(2021) Tanya T Paull   CURRENT OPINION IN GENETICS & DEVELOPMENT

Autophosphorylation transforms DNA-PK from protecting to processing DNA ends

(2021) Lan Liu et al.   MOLECULAR CELL

Polymerase μ in non-homologous DNA end joining: importance of the order of arrival at a double-strand break in a purified system

(2020) Bailin Zhao et al.   NUCLEIC ACIDS RESEARCH

Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge

(2020) Hisashi Tatebe et al.   Nature Communications

ATM, ATR and DNA-PKcs kinases—the lessons from the mouse models: inhibition ≠ deletion

(2020) Demis Menolfi et al.   Cell and Bioscience

Roles for the DNA-PK complex and 53BP1 in protecting ends from resection during DNA double-strand break repair

(2020) Atsushi Shibata et al.   JOURNAL OF RADIATION RESEARCH

The molecular basis and disease relevance of non-homologous DNA end joining

(2020) Bailin Zhao et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Dimers of DNA-PK create a stage for DNA double-strand break repair

(2020) Amanda K. Chaplin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Uncovering DNA-PKcs ancient phylogeny, unique sequence motifs and insights for human disease

(2020) James P. Lees-Miller et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break

(2020) Andrea M. Kaminski et al.   Nature Communications

DNA-PKcs: A Multi-Faceted Player in DNA Damage Response

(2020) Xiaoqiao Yue et al.   Frontiers in Genetics

PARP-1 Activation Directs FUS to DNA Damage Sites to Form PARG-Reversible Compartments Enriched in Damaged DNA

(2019) Anastasia S. Singatulina et al.   Cell Reports

Nej1 Interacts with Mre11 to Regulate Tethering and Dna2 Binding at DNA Double-Strand Breaks

(2019) Aditya Mojumdar et al.   Cell Reports

The essential elements for the noncovalent association of two DNA ends during NHEJ synapsis

(2019) Bailin Zhao et al.   Nature Communications

Ligand binding characteristics of the Ku80 von Willebrand domain

(2019) Kyungmin Kim et al.   DNA REPAIR

A Mechanism to Minimize Errors during Non-homologous End Joining

(2019) Benjamin M. Stinson et al.   MOLECULAR CELL

Repair of DNA double-strand breaks by mammalian alternative end-joining pathways

(2018) Annahita Sallmyr et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Homologous recombination and the repair of DNA double-strand breaks

(2018) William Douglass Wright et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dissection of DNA double-strand-break repair using novel single-molecule forceps

(2018) Jing L. Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structures of DNA-bound human ligase IV catalytic core reveal insights into substrate binding and catalysis

(2018) Andrea M. Kaminski et al.   Nature Communications

Dissection of DNA double-strand-break repair using novel single-molecule forceps

(2018) Jing L. Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining

(2018) Clement Nemoz et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-particle cryo-EM—How did it get here and where will it go

(2018) Yifan Cheng   SCIENCE

Autoinhibition of the Nuclease ARTEMIS Is Mediated by a Physical Interaction between Its Catalytic and C-terminal Domains

(2017) Doris Niewolik et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and functional characterization of the PNKP–XRCC4–LigIV DNA repair complex

(2017) R. Daniel Aceytuno et al.   NUCLEIC ACIDS RESEARCH

Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu

(2017) Andrea F. Moon et al.   NUCLEIC ACIDS RESEARCH

Cryo-EM structure of the DNA-PK holoenzyme

(2017) Humayun Sharif et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of closed and open conformations of dimeric human ATM

(2017) Domagoj Baretić et al.   Science Advances

Structure of the human dimeric ATM kinase

(2016) Wilson C. Y. Lau et al.   CELL CYCLE

An Intrinsically Disordered APLF Links Ku, DNA-PKcs, and XRCC4-DNA Ligase IV in an Extended Flexible Non-homologous End Joining Complex

(2016) Michal Hammel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Two-Stage Synapsis of DNA Ends during Non-homologous End Joining

(2016) Thomas G.W. Graham et al.   MOLECULAR CELL

VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break Repair

(2016) Johannes van den Boom et al.   MOLECULAR CELL

Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA

(2016) Ineke Brouwer et al.   NATURE

Bridging of double-stranded breaks by the nonhomologous end-joining ligation complex is modulated by DNA end chemistry

(2016) Dylan A. Reid et al.   NUCLEIC ACIDS RESEARCH

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF

(2016) Satish K. Tadi et al.   Cell Reports

Functions of PARylation in DNA Damage Repair Pathways

(2016) Huiting Wei et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair

(2015) A Craxton et al.   CELL DEATH AND DIFFERENTIATION

Versatility in phospho-dependent molecular recognition of the XRCC1 and XRCC4 DNA-damage scaffolds by aprataxin-family FHA domains

(2015) Amy L. Cherry et al.   DNA REPAIR

Unifying the DNA End-processing Roles of the Artemis Nuclease

(2015) Howard H. Y. Chang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair

(2015) T. Ochi et al.   SCIENCE

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway

(2015) Mengtan Xing et al.   Nature Communications

Neddylation Promotes Ubiquitylation and Release of Ku from DNA-Damage Sites

(2015) Jessica S. Brown et al.   Cell Reports

Sources of DNA Double-Strand Breaks and Models of Recombinational DNA Repair

(2014) A. Mehta et al.   Cold Spring Harbor Perspectives in Biology

Modernizing the Nonhomologous End-Joining Repertoire: Alternative and Classical NHEJ Share the Stage

(2013) Ludovic Deriano et al.   Annual Review of Genetics

TRF2 Interaction with Ku Heterotetramerization Interface Gives Insight into c-NHEJ Prevention at Human Telomeres

(2013) Albert Ribes-Zamora et al.   Cell Reports

TDP2–Dependent Non-Homologous End-Joining Protects against Topoisomerase II–Induced DNA Breaks and Genome Instability in Cells and In Vivo

(2013) Fernando Gómez-Herreros et al.   PLoS Genetics

The dynamics of Ku70/80 and DNA-PKcs at DSBs induced by ionizing radiation is dependent on the complexity of damage

(2012) Pamela Reynolds et al.   NUCLEIC ACIDS RESEARCH

Functional significance of the interaction with Ku in DNA double-strand break recognition of XLF

(2011) Ken-ichi Yano et al.   FEBS LETTERS

XRCC4 Protein Interactions with XRCC4-like Factor (XLF) Create an Extended Grooved Scaffold for DNA Ligation and Double Strand Break Repair

(2011) Michal Hammel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA Repair Factor APLF Is a Histone Chaperone

(2011) Pawan Vinod Mehrotra et al.   MOLECULAR CELL

Phosphorylation of polynucleotide kinase/ phosphatase by DNA-dependent protein kinase and ataxia-telangiectasia mutated regulates its association with sites of DNA damage

(2011) Angela E. Zolner et al.   NUCLEIC ACIDS RESEARCH

Structural characterization of filaments formed by human Xrcc4-Cernunnos/XLF complex involved in nonhomologous DNA end-joining

(2011) V. Ropars et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates

(2011) N. Coquelle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Release of Ku and MRN from DNA Ends by Mre11 Nuclease Activity and Ctp1 Is Required for Homologous Recombination Repair of Double-Strand Breaks

(2011) Petra Langerak et al.   PLoS Genetics

Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks

(2010) Anthony J. Davis et al.   CELL CYCLE

DNA-PKcs regulates a single-stranded DNA endonuclease activity of Artemis

(2010) Jiafeng Gu et al.   DNA REPAIR

Focus on histone variant H2AX: To be or not to be

(2010) Jingsong Yuan et al.   FEBS LETTERS

A chromatin localization screen reveals poly (ADP ribose)-regulated recruitment of the repressive polycomb and NuRD complexes to sites of DNA damage

(2010) D. M. Chou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinct kinetics of human DNA ligases I, IIIα, IIIβ, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair

(2009) Xi Chen et al.   DNA REPAIR

Artemis Regulates Cell Cycle Recovery from the S Phase Checkpoint by Promoting Degradation of Cyclin E

(2009) Haiyong Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and Functional Interaction between the Human DNA Repair Proteins DNA Ligase IV and XRCC4

(2009) P.-Y. Wu et al.   MOLECULAR AND CELLULAR BIOLOGY

Crystal structure of DNA-PKcs reveals a large open-ring cradle comprised of HEAT repeats

(2009) Bancinyane L. Sibanda et al.   NATURE

Role of mammalian Mre11 in classical and alternative nonhomologous end joining

(2009) Anyong Xie et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Werner Protein Cooperates with the XRCC4-DNA Ligase IV Complex in End-Processing†

(2008) Rika Kusumoto et al.   BIOCHEMISTRY

A comparison of BRCT domains involved in nonhomologous end-joining: Introducing the solution structure of the BRCT domain of polymerase lambda

(2008) Geoffrey A. Mueller et al.   DNA REPAIR

XLF-Cernunnos promotes DNA ligase IV–XRCC4 re-adenylation following ligation

(2008) Enriqueta Riballo et al.   NUCLEIC ACIDS RESEARCH