Cryo-EM of NHEJ supercomplexes provides insights into DNA repair

Structure of an activated DNA-PK and its implications for NHEJ

(2021) Xuemin Chen et al.   MOLECULAR CELL

The active DNA-PK holoenzyme occupies a tensed state in a staggered synaptic complex

(2021) Morgan Hepburn et al.   STRUCTURE

Structural basis of long-range to short-range synaptic transition in NHEJ

(2021) Siyu Chen et al.   NATURE

3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM

(2021) Ali Punjani et al.   JOURNAL OF STRUCTURAL BIOLOGY

Activation of DNA-PK by hairpinned DNA ends reveals a stepwise mechanism of kinase activation

(2020) Katheryn Meek   NUCLEIC ACIDS RESEARCH

Dimers of DNA-PK create a stage for DNA double-strand break repair

(2020) Amanda K. Chaplin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

XLF acts as a flexible connector during non-homologous end joining

(2020) Sean M Carney et al.   eLife

Namdinator – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

(2019) Rune Thomas Kidmose et al.   IUCrJ

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Dissection of DNA double-strand-break repair using novel single-molecule forceps

(2018) Jing L. Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dissection of DNA double-strand-break repair using novel single-molecule forceps

(2018) Jing L. Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

A single XLF dimer bridges DNA ends during nonhomologous end joining

(2018) Thomas G. W. Graham et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining

(2018) Clement Nemoz et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Building Proteins in a Day: Efficient 3D Molecular Structure Estimation with Electron Cryomicroscopy

(2017) Ali Punjani et al.   IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Cryo-EM structure of the DNA-PK holoenzyme

(2017) Humayun Sharif et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Restoration of ATM Expression in DNA-PKcs–Deficient Cells Inhibits Signal End Joining

(2016) Jessica A. Neal et al.   JOURNAL OF IMMUNOLOGY

Two-Stage Synapsis of DNA Ends during Non-homologous End Joining

(2016) Thomas G.W. Graham et al.   MOLECULAR CELL

Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA

(2016) Ineke Brouwer et al.   NATURE

The Ku-binding motif is a conserved module for recruitment and stimulation of non-homologous end-joining proteins

(2016) Gabrielle J. Grundy et al.   Nature Communications

Non-homologous end-joining partners in a helical dance: structural studies of XLF–XRCC4 interactions

(2012) Qian Wu et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

XRCC4 and XLF form long helical protein filaments suitable for DNA end protection and alignment to facilitate DNA double strand break repair

(2012) Brandi L. Mahaney et al.   Biochemistry and Cell Biology

XRCC4 Protein Interactions with XRCC4-like Factor (XLF) Create an Extended Grooved Scaffold for DNA Ligation and Double Strand Break Repair

(2011) Michal Hammel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural characterization of filaments formed by human Xrcc4-Cernunnos/XLF complex involved in nonhomologous DNA end-joining

(2011) V. Ropars et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Coordination of DNA–PK Activation and Nuclease Processing of DNA Termini in NHEJ

(2010) Katherine S Pawelczak et al.   ANTIOXIDANTS & REDOX SIGNALING

Delineation of the Xrcc4-interacting Region in the Globular Head Domain of Cernunnos/XLF

(2010) Laurent Malivert et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Biochemically Defined System for Coding Joint Formation in V(D)J Recombination

(2008) Haihui Lu et al.   MOLECULAR CELL

A mechanism for DNA-PK activation requiring unique contributions from each strand of a DNA terminus and implications for microhomology-mediated nonhomologous DNA end joining

(2008) Katherine S. Pawelczak et al.   NUCLEIC ACIDS RESEARCH